Ángela María Candreva, Paola L Smaldini, Ana Cauerhff, Silvana Petruccelli, Guillermo H Docena
Food allergy is on the rise, and preventive/therapeutic procedures are needed. We explored a preventive protocol for milk allergy with the oral administration of a Gly-m-Bd-30K soy-derived peptide that contains cross-reactive epitopes with bovine caseins. B/T-cross-reactive epitopes were mapped using milk-specific human sera and monoclonal antibodies on overlapping and recombinant peptides of Gly-m-Bd-30K by SPOT and cell proliferation assays. Bioinformatics tools were used to characterize epitopes on the 3D-modelled molecule, and to predict the binding to HLA alleles...
December 26, 2020: Food Chemistry
Anna Maria Ogrodowczyk, Ivan Dimitrov, Barbara Wróblewska
The main food-origin antigens that the infant's body is in contact with are cow's milk proteins (CMP). Still, CMP are one of the main sources of beneficial biologically active peptides that play a role in treatment of non-communicable diseases. Safe methods to quickly predict the sensitizing potential of food proteins among their range of health-promoting properties are essential. The aim of study was to adapt an integrated approach combining several in silico ( IS ) studies and in vitro ( IV ) assays to screen the multifunctionality of CMP-derived peptides...
January 14, 2021: Foods (Basel, Switzerland)
Sebastián Castro, Carlos Velasco Suárez, Ana Vieites, Ignacio Bergadá, Hamilton Cassinelli
The rickets is a disease that affects the differentiation and mineralization of the growth cartilage, as an ultimate consequence of a balance loss in calcium and phosphate levels. Vitamin D deficiency is the most common cause of the rickets (nutritional rickets). Its clinical manifestation during the first years of life involves long bones epiphysis in a more severe way. We report an 8-month-old infant who was diagnosed with cow´s milk protein allergy and suffered from multiple fractures while receiving elemental formula as part of his treatment...
February 2021: Archivos Argentinos de Pediatría
Carlett Ramirez-Farias, Geraldine E Baggs, Barbara J Marriage
BACKGROUND: The purpose of this study was to evaluate the growth, tolerance and compliance effects of an extensively hydrolyzed formula with added 2'-FL in an intended use population of infants. METHODS: A non-randomized, single-group, multicenter study was conducted. Infants (0-60 days of age) with suspected food protein allergy, persistent feeding intolerance, or presenting conditions where an extensively hydrolyzed formula (eHF) was deemed appropriate were enrolled in a 2-month feeding trial...
January 9, 2021: Nutrients
Mark Smits, Irene Nooijen, Frank Redegeld, Aard de Jong, Thuy-My Le, André Knulst, Geert Houben, Kitty Verhoeckx
SCOPE: No accepted and validated methods are currently available which can accurately predict protein allergenicity. In this study, the role of digestion and transport on protein allergenicity was investigated METHODS AND RESULTS: Peanut allergens (Ara h 1, 2, 3, and 6) and a milk allergen (β-lactoglobulin) were transported across pig intestinal epithelium using the InTESTine™ model and afterwards basophil activation was measured to assess the (remaining) functional properties. Additionally, allergens were digested by pepsin prior to epithelial transport and their allergenicity was assessed in a human mast cell activation assay...
January 12, 2021: Molecular Nutrition & Food Research
Erick M Toro-Monjaraz, Gabriela Fonseca-Camarillo, Flora Zárate-Mondragón, Ericka Montijo-Barrios, José Cadena-León, David Avelar-Rodríguez, Jaime Ramírez-Mayans, Roberto Cervantes-Bustamante, Jesús K Yamamoto-Furusho
BACKGROUND: Cow's milk protein allergy (CMPA) is the most prevalent food allergy in children, and its pathogenesis remains poorly understood. It has been shown that the combination of genetic predisposition, perinatal factors, and intestinal imbalance of the immune response mediated by cytokines may play an essential role in CMPA pathogenesis. AIM: To characterize the gene expression of Th1, Th2, and Th17 cytokines in the duodenum and rectum in patients with CMPA...
January 12, 2021: Digestive Diseases and Sciences
Amanda L Cox, Philippe A Eigenmann, Scott H Sicherer
The diagnosis and management of food allergy is complicated by an abundance of homologous, cross-reactive proteins in edible foods and aeroallergens. This results in patients having allergic sensitization (positive tests) to many biologically related foods. However, many are sensitized to foods without exhibiting clinical reactivity. Although molecular diagnostics have improved our ability to identify clinically relevant cross-reactivity, the optimal approach to patients requires an understanding of the epidemiology of clinically relevant cross-reactivity, as well as the food-specific (degree of homology, protein stability, abundance) and patient-specific factors (immune response, augmentation factors) that determine clinical relevance...
January 2021: Journal of Allergy and Clinical Immunology in Practice
Nazar Hussain, Ran Li, Timo M Takala, Muhammad Tariq, Arsalan H Zaidi, Per E J Saris
Lacticaseibacillus rhamnosus GG (LGG) is the most studied probiotic bacterium in the world. It is used as a probiotic supplement in many foods, including various dairy products. However, LGG grows poorly in milk, as it neither metabolizes the main milk carbohydrate lactose, nor degrades the major milk protein casein effectively. In this study, we made L. rhamnosus GG lactose and protease positive by conjugation with the dairy Lactococcus lactis strain NCDO 712 carrying the lactose-protease plasmid pLP712. A lactose hydrolyzing transconjugant colony was obtained on agar containing lactose as the sole source of carbohydrates...
January 8, 2021: Applied and Environmental Microbiology
E Lee, E H Barnes, S Mehr, D E Campbell
BACKGROUND: Food protein-induced enterocolitis syndrome (FPIES) is a non-IgE-mediated food allergic disorder with a well-characterised phenotype, but limited understanding of factors associated with food cross-reactivity, severity and tolerance. METHODS: A retrospective cohort study spanning 20 years on children with acute FPIES from a single paediatric tertiary centre in New South Wales, Australia focusing on identifying food trigger co-associations and factors associated with reaction severity, multiple trigger FPIES and/or tolerance was performed...
January 7, 2021: Pediatric Allergy and Immunology
Ana Muñoz-Urribarri, Aderbal Sabrá, Selmá Sabrá, Yovani M Condorhuamán
This study presents an anamnesis-based questionnaire as a diagnostic tool for cow's milk protein allergy (CMPA) in children. We applied 24 dichotomous yes/no questions to 51 cases diagnosed by oral challenge and 31 controls. All patients were recruited at the pediatric gastroenterologist outpatient practice. CMPA patients presented with a family history of atopy/autoimmunity, Caesarean delivery, use and/or change of formulas, use of antacids/antibiotics in the first 6 months of life, an overly clean caregiver, multisystem clinical presentation, and the absence of seasonal symptoms...
December 23, 2020: Journal of Pediatric Gastroenterology and Nutrition
Maurizio Mennini, Stefania Arasi, Alessandro Giovanni Fiocchi
PURPOSE OF REVIEW: To perform a nonsystematic review of the literature on the role of breastfeeding as primary prevention tool for allergic diseases. RECENT FINDINGS: Human milk contains vast amounts of biologically active components that have a significant impact on the development of the gut microbiota. Exclusively breastfed infants show a different microbiota, with a predominance of Bifidobacterium species in their intestines.The mechanisms underlying the antiallergic effects of human milk are most probably complex, as human milk contains not only nutritional substances but also functional molecules including polysaccharides, cytokines, proteins, and other components which can produce an epigenetic modulation of the innate and adaptive immune responses of the infant in very early life...
December 30, 2020: Current Opinion in Allergy and Clinical Immunology
Hisako Yagi, Koichiro Sato, Naoya Arakawa, Takaharu Inoue, Yutaka Nishida, Satoshi Yamada, Takashi Ishige, Yoshiyuki Yamada, Hirokazu Arakawa, Takumi Takizawa
The lymphocyte stimulation test (LST) facilitates the diagnosis of non-IgE-mediated gastrointestinal food allergies (non-IgE-GI-FAs). However, LSTs require large volumes of blood and prolonged culture durations. Recently, we found that IL2RA mRNA expression in peripheral blood mononuclear cells (PBMCs) of patients with non-IgE-GI-FAs increased after a 24 h stimulation with milk proteins. We designated this gene expression test as the instant peripheral blood allergen stimulation test (iPAST). In this study, we investigated whether other activated T cell-associated genes are superior to IL2RA in the iPAST for the supplementary diagnosis of non-IgE-GI-FAs...
December 2020: Yale Journal of Biology and Medicine
Kehong Chen, Atsushi Murao, Adnan Arif, Satoshi Takizawa, Hui Jin, Jianxin Jiang, Monowar Aziz, Ping Wang
Phagocytic clearance of apoptotic cells by the macrophages (efferocytosis) is impaired in sepsis, but its mechanism is poorly understood. Extracellular cold-inducible RNA-binding protein (eCIRP) is a novel damage-associated molecular pattern that fuels inflammation. We identify that eCIRP-induced neutrophil extracellular traps (NETs) impair efferocytosis through a novel mechanism. Coculture of macrophages and apoptotic thymocytes in the presence of recombinant murine CIRP (rmCIRP)-induced NETs significantly inhibited efferocytosis...
December 30, 2020: Journal of Immunology
Ludmila Kalčáková, Matej Pospiech, Bohuslava Tremlová, Zdeňka Javůrková, Irina Chernukha
To increase production efficiency of meat products, milk protein additives are often used. Despite a number of advantages, use of dairy ingredients involves a certain risk, namely the allergenic potential of milk proteins. A number of methods have been developed to detect milk-origin raw materials in foodstuffs, including immunological reference methods. This study presents newly developed immunohistochemical (IHC) methods for casein detection in meat products. Casein was successfully detected directly in meat products where sensitivity was determined at 1...
December 24, 2020: Foods (Basel, Switzerland)
Michaela M Hansen, Susanne P Nissen, Susanne Halken, Arne Høst
BACKGROUND: Previous studies have investigated the natural course of cow's milk allergy (CMA) and development of atopic diseases into adolescence. Studies with long term follow-up into adulthood are lacking. The aim of this study was to investigate 1) the natural course of CMA in a 1-year birth cohort of Danish children from birth until 15 and 26 years of age and 2) the development of atopic diseases in a group of children with CMA (group A) compared to a random sample of 276 children from the same birth cohort (group B)...
December 22, 2020: Pediatric Allergy and Immunology
Goutham Vansarla, Anders P Håkansson, Caroline Bergenfelz
HAMLET is a protein-lipid complex with a specific and broad bactericidal and tumoricidal activity, that lacks cytotoxic activity against healthy cells. In this study, we show that HAMLET also has general immune-stimulatory effects on primary human monocyte-derived dendritic cells and macrophages (Mo-DC and Mo-M) and murine RAW264.7 macrophages. HAMLET, but not its components alpha-lactalbumin or oleic acid, induces mature CD14low/- CD83+ Mo-DC and M1-like CD14+ CD86++ Mo-M surface phenotypes. Concomitantly, inflammatory mediators, including IL-2, IL-6, IL-10, IL-12 and MIP-1α, were released in the supernatant of HAMLET-stimulated cells, indicating a mainly pro-inflammatory phenotype...
December 21, 2020: European Journal of Immunology
Elvira Verduci, Elisabetta Di Profio, Lucia Cerrato, Giulia Nuzzi, Luca Riva, Giulia Vizzari, Enza D'Auria, Maria Lorella Giannì, Gianvincenzo Zuccotti, Diego G Peroni
Soybean ( Glycine max ) is a species of legume native to East Asia and used in childhood diet for over 2,000 years in the East. Soy protein formulas have been available for almost a century. Nowadays, the increase in cow's milk allergy and vegetarian dietary preferences are driving consumers toward cow's milk alternatives. In this paper, we reviewed the nutritional composition of soy-based infant formula and discussed their possible use in pediatric age, mainly focusing on prevention and treatment of cow's milk allergy...
2020: Frontiers in Pediatrics
Zaid Siddique, Ryan Thibodeau, Abtin Jafroodifar, Ravikumar Hanumaiah
Hepatic portal venous gas (HPVG) is a rare imaging finding in infants and usually indicative of a severe disease process such as necrotizing enterocolitis, bowel ischemia, or bowel wall rupture / infarction. The diagnosis of HPVG may have serious implications such as parenteral nutrition, antibiotics and even surgery. In this case, we present an 8-week-old male with a history of prematurity presenting with HPVG, later concluded to be caused by milk protein allergy. Milk protein allergy is a rare cause of HPVG, but it should be recognized due to its benignity and potential prevention of unnecessary testing and interventions...
February 2021: Radiology Case Reports
Jing Wang, Ying Zhang, Hong Li, Guoqing Chen, Yuqiong Zou, Kathe Rin
β-lactoglobulin (β-Lg) allergy in dietary milk seriously affects the use of high-quality milk protein in infants. In order to solve this problem, the expression of miRNA and Th17 cells in milk β-Lg allergic reaction of children's diet was studied. METHOD: female BALB/c mice aged 5-6 weeks were selected as the subjects and randomly divided into blank group and β-Lg sensitized group, with 10 mice in each group. On the 1st, 7th and 14th day, the mice in the β-Lg sensitized group were intraperitoneally injected with allergen (Freund's adjuvant + β-Lg)...
December 2020: Saudi Journal of Biological Sciences
Dimuthu Bogahawaththa, Todor Vasiljevic
This research communication relates to the hypothesis that the consumption of raw or unprocessed cow's milk contributes to lowered prevalence of allergies. Thermal pasteurization of bovine milk can result in denaturation of minor whey proteins and loss of their bioactivity. Denaturation of bovine serum albumin (BSA), immunoglobulin G (IgG) and lactoferrin (LF) in skim milk was studied under different temperature (72, 75 or 78°C) and time (0-300 s) combinations. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) results revealed that denaturation of all 3 proteins occurred at 72°C and progressed with increase in temperature and holding time...
November 2020: Journal of Dairy Research
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