Viviana Escudero, Isidro Abreu, Manuel Tejada-Jiménez, Elena Rosa-Núñez, Julia Quintana, Rosa Isabel Prieto, Camille Larue, Jiangqi Wen, Julie Villanova, Kirankumar S Mysore, José M Argüello, Hiram Castillo-Michel, Juan Imperial, Manuel González-Guerrero
Iron is an essential cofactor for symbiotic nitrogen fixation, required by many of the enzymes involved, including signal transduction proteins, O2 homeostasis systems, and nitrogenase itself. Consequently, host plants have developed a transport network to deliver essential iron to nitrogen-fixing nodule cells. Ferroportin family members in model legume Medicago truncatula were identified and their expression was determined. Yeast complementation assays, immunolocalization, characterization of a tnt1 insertional mutant line, and synchrotron-based X-ray fluorescence assays were carried out in the nodule-specific M...
May 4, 2020: New Phytologist