Philippe J Eugster, Jonathan Maurer, Céline Vocat, Karim Abid, Maurice Matter, Grégoire Wuerzner, Roman Trepp, Stefan Fischli, Christoph Henzen, Walter Kolb, Stefan Bilz, Sarah Sigrist, Felix Beuschlein, Svenja Nölting, Astrid Reul, Ina Schütze, Scott A Hubers, Nancy J Brown, Eric Grouzmann
Neuropeptide Y (NPY1-36) is a vasoconstrictor peptide co-secreted with catecholamines by sympathetic nerves, the adrenal medulla, and neoplasms such as pheochromocytomas and paragangliomas (PPGLs). It is produced by the intracellular cleavage of proNPY and metabolized into multiple fragments with distinct biological activities. NPY immunoassays for PPGL have a diagnostic sensitivity ranging from 33 to 100%, depending on the antibody used. We have validated a multiplex micro-UHPLC-MS/MS assay for the specific and sensitive quantification of proNPY, NPY1-39, NPY1-37, NPY1-36, NPY2-36, NPY3-36, NPY1-35, NPY3-35, and the C-flanking peptide of NPY (CPON) (collectively termed NPYs), and determined the NPYs reference intervals and concentrations in 32 PPGL patients before, during, and after surgery...
September 1, 2022: Clinica Chimica Acta; International Journal of Clinical Chemistry