Shuxian Li, Yabin Lu, Shanshan Yang, Caiying Wang, Jing Yang, Xin Huang, Guohui Chen, Yongheng Shao, Maolin Li, Haoyuan Yu, Yuguang Fu, Guangliang Liu
Respiratory coronaviruses (RCoVs) significantly threaten human health, necessitating the development of an ex vivo respiratory culture system for investigating RCoVs infection. Here, we successfully generated a porcine precision-cut lung slices (PCLSs) culture system, containing all resident lung cell types in their natural arrangement. Next, this culture system was inoculated with a porcine respiratory coronavirus (PRCV), exhibiting clinical features akin to humans who were infected by SARS-CoV-2. The results demonstrated that PRCV efficiently infected and replicated within PCLSs, targeting ciliated cells in the bronchioles, terminal bronchioles, respiratory bronchioles, and pulmonary alveoli...
May 16, 2024: AMB Express