Tanner Stokes, Haoning Howard Cen, Philipp Kapranov, Iain J Gallagher, Andrew A Pitsillides, Claude-Henry Volmar, William E Kraus, James D Johnson, Stuart M Phillips, Claes Wahlestedt, James A Timmons
Sequencing the human genome empowers translational medicine, facilitating transcriptome-wide molecular diagnosis, pathway biology, and drug repositioning. Initially, microarrays are used to study the bulk transcriptome; but now short-read RNA sequencing (RNA-seq) predominates. Positioned as a superior technology, that makes the discovery of novel transcripts routine, most RNA-seq analyses are in fact modeled on the known transcriptome. Limitations of the RNA-seq methodology have emerged, while the design of, and the analysis strategies applied to, arrays have matured...
June 2023: Advanced genetics