keyword
https://read.qxmd.com/read/34319629/visual-typing-detection-of-brucellosis-with-a-lateral-flow-immunoassay-based-on-coloured-latex-microspheres
#1
Feng Shi, Yan Tang, Zhi-Hua Xu, Yi-Xiao Sun, Ming-Ze Ma, Chuang-Fu Chen
AIMS: Treatment and preventive control strategies for Brucella melitensis (B. melitensis) and Brucella abortus (B. abortus) infection differ. A lateral flow immunoassay (LFIA) for the rapid typing and detection of brucellosis by using polychromatic dye-doped latex microspheres (LMs) as a labelling material was developed. METHODS AND RESULTS: This LFIA utilize a double-antigen sandwich method in which the BP26 protein is used as the diagnostic antigen to detect brucellosis infection and the OMP31 protein is used as the identified antigen to distinguish bovine and sheep brucellosis...
July 28, 2021: Journal of Applied Microbiology
https://read.qxmd.com/read/34307536/presumptive-identification-of-smooth-brucella-strain-antibodies-in-canines
#2
Alyssa B Helms, Orsolya Balogh, Rebecca Franklin-Guild, Kevin Lahmers, Clayton C Caswell, Julie T Cecere
Brucellosis is a zoonotic disease caused by a Gram-negative coccobacillus. There are four Brucella strains of zoonotic importance in our domestic species, subdivided by their culture phenotypes: Brucella abortus ( B. abortus ), B. melitensis, B. suis (smooth strains) and B. canis (rough strain). Dogs can serve as hosts for all four of the zoonotic strains; however, routine serologic testing in dogs has been limited to the identification of B. canis antibodies. The aim of our study was to identify smooth Brucella strain antibodies in canines...
2021: Frontiers in Veterinary Science
https://read.qxmd.com/read/34305935/mucosal-vaccination-primes-nk-cell-dependent-development-of-cd8-t-cells-against-pulmonary-brucella-infection
#3
Ella Bhagyaraj, Hongbin Wang, Xinghong Yang, Carol Hoffman, Ali Akgul, Zakia I Goodwin, David W Pascual
Past studies with the live, double-mutant  B. abortus  (znBAZ) strain resulted in nearly complete protection of mice against pulmonary challenge with wild-type (wt) Brucella via a dominant CD8+ T cell response. To understand the contribution innate immune cells in priming CD8+ T cell responses, mice were nasally dosed with wt B. abortus , smooth vaccine strain 19 (S19), or znBAZ, and examined for innate immune cell activation. Flow cytometric analysis revealed that znBAZ, but not wt B. abortus nor S19 infection, induces up to a 5-fold increase in the frequency of IFN-γ-producing NK cells in mouse lungs...
2021: Frontiers in Immunology
https://read.qxmd.com/read/34290993/registered-influenza-viral-vector-based-brucella-abortus-vaccine-for-cattle-in-kazakhstan-age-wise-safety-and-efficacy-studies
#4
Sholpan Ryskeldinova, Nadezhda Zinina, Zhailaubay Kydyrbayev, Bolat Yespembetov, Yerken Kozhamkulov, Dulat Inkarbekov, Nurika Assanzhanova, Aigerim Mailybayeva, Dina Bugybayeva, Makhpal Sarmykova, Berik Khairullin, Kairat Tabynov, Aitbay Bulashev, Batyrbek Aitzhanov, Khairulla Abeuov, Abylay Sansyzbay, Tlektes Yespolov, Gourapura J Renukaradhya, Steven Olsen, Angel Oñate, Kaissar Tabynov
A novel influenza viral vector based Brucella abortus vaccine (Flu-BA) was introduced for use in cattle in Kazakhstan in 2019. In this study, the safety and efficacy of the vaccine was evaluated in male and female cattle at different ages, and during pregnancy as a part of its registration process. Our data demonstrated that the Flu-BA vaccine was safe after prime or booster vaccination in calves (5-7 months old male and female), heifers (15-17 months old) and cows (6-7 years old) and was not abortogenic in pregnant animals...
2021: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/34287457/determination-of-antimicrobial-and-antimutagenic-properties-of-some-schiff-bases
#5
Hatice Ogutcu, Seher Meral, Selcuk Ceker, Aysen Alaman Agar, Guleray Agar
In this study, we aimed to investigate for the first time antimicrobial and antimutagenic activities new two Schiff bases, obtained from a primary amine (p-toluidine, o-toluidine) and an aldehyde (Helicin). Synthesized compounds characterized with elemental analysis, fourier transform infrared spectroscopy, ultraviolet-visible spectrophotometry. 1H-13C nuclear magnetic resonance spectroscopy. Antimutagenic activity was evaluated by micronuclei assay. Antimicrobial activity of Schiff bases have been demonstrated against pathogenic four Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermis, Micrococcus luteus, Bacillus cereus) and four Gram-negative bacteria (Pseudumonas aeroginosa, Salmonella typhi H, Brucella abortus, Escherichia coli) and two yeasts (Candida albicans and Saccharomyces cerevisiae)...
2021: Anais da Academia Brasileira de Ciências
https://read.qxmd.com/read/34283853/seroprevalence-of-brucellosis-and-q-fever-infections-amongst-pastoralists-and-their-cattle-herds-in-sokoto-state-nigeria
#6
Simeon Cadmus, Samson Polycarp Salam, Hezekiah Kehinde Adesokan, Kelechi Akporube, Fiyinfoluwa Ola-Daniel, Emmanuel Jolaoluwa Awosanya
Brucellosis and Q fever are neglected zoonoses of global health importance, with unknown true prevalence in occupationally vulnerable settings, partly due to misdiagnosis for other febrile conditions and poor access to primary health care. We examined the seroprevalence of these diseases and associated factors amongst pastoralists and their cattle in Sokoto State, a hub of cattle and pastoral populations in Nigeria. Serum samples randomly collected from 137 pastoralists and 366 cattle from 27 herds in three selected Local Government Areas (LGAs) in the state were analysed for antibodies to Brucella abortus using Rose Bengal Plate Test (RBT) and competitive Enzyme-Linked Immunosorbent Assay (cELISA) as well as antibodies to Coxiella burnetti using indirect ELISA...
2021: PloS One
https://read.qxmd.com/read/34260490/an-adolescent-with-neurobrucellosis-caused-by-brucella-abortus-cattle-vaccine-strain-rb51
#7
Adriana Sarmiento Clemente, Megan H Amerson-Brown, Catherine E Foster
We present the case of an 18-year-old female with a 1-month history of fever, headache, and double vision, whose examination revealed papilledema and cranial nerve VI palsy. Blood cultures grew Brucella abortus cattle vaccine strain RB51, which is inherently resistant to rifampin. We discuss the management of the first known case of neurobrucellosis by this strain.
July 13, 2021: Pediatric Infectious Disease Journal
https://read.qxmd.com/read/34245824/immune-metabolic-receptor-gpr84-surrogate-and-endogenous-agonists-6-oau-and-lauric-acid-alter-brucella-abortus-544-infection-in-both-in-vitro-and-in-vivo-systems
#8
Alisha Wehdnesday Bernardo Reyes, Heejin Kim, Tran Xuan Ngoc Huy, Son Hai Vu, Trang Thi Nguyen, Chang Keun Kang, Wongi Min, Hu Jang Lee, John Hwa Lee, Suk Kim
Brucella abortus, one of the most important members of the genus Brucella responsible for human disease, is an intracellular pathogen capable of avoiding or interfering components of the host immune responses that are critical for its virulence. GPR84, on the other hand, is a seven-transmembrane GPCR involved in the inflammatory response and its induced expression was associated with B. abortus infection of RAW264.7 cells. Here we examined the effects of the reported GPR84 surrogate and endogenous agonists, namely 6-n-octylaminouracil (6-OAU) and lauric acid (LU), respectively in the progression of B...
July 7, 2021: Microbial Pathogenesis
https://read.qxmd.com/read/34245235/genotyping-of-brucella-strains-isolated-from-humans-and-cattle-of-different-geographical-regions-of-pakistan-using-mlva-15
#9
Raheela Akhtar, Muhammad Muddassir Ali, Asad Ullah, Abdul Muttalib, Kashan Mehboob, Arif Ullah, Nasim Ahmad, Tahir Zahoor Chohan
BACKGROUND: Prevalence of brucellosis and MLVA genotyping in animals and humans, isolated from different regions of Pakistan was performed. Animals having history of brucellosis from the field and local farms of Bannu, Mardan, Peshawar, Swat, Lahore and Islamabad were selected for blood collection. Humans that work with them were also selected for sampling in this study. Total of 600 samples were taken from cattle and humans and subjected to Rose Bengal plate Test (RBPT) for the initial screening of positive samples...
July 10, 2021: Veterinary Medicine and Science
https://read.qxmd.com/read/34212019/a-retrospective-survey-of-the-abortion-outbreak-event-caused-by-brucellosis-at-a-blue-fox-breeding-farm-in-heilongjiang-province-china
#10
Yulong Zhou, Ye Meng, Yachao Ren, Zhiguo Liu, Zhenjun Li
Brucellosis is a common zoonosis in China, resulting in abortion in animals. Outbreaks of abortion in blue foxes caused by Brucella infection have rarely been reported. In the present study, 3-5 mL blood samples collected from the femoral veins of 10 abortuses of blue foxes were assessed by RBPT (Rose Bengal plate test) and SAT (serum tube agglutination test) to preliminarily investigate the source of infection for the clustering of abortion events at a blue fox farm in Heilongjiang Province. Screening experiments showed that all 10 blood samples were positive in the RBPT, while only eight blood samples out of the 10 were positive in the SAT...
2021: Frontiers in Veterinary Science
https://read.qxmd.com/read/34211345/molecular-detection-of-zoonotic-pathogens-in-the-blood-and-tissues-of-camels-camelus-dromedarius-in-central-desert-of-iran
#11
Aliasghar Bahari, Sakineh Azami, Ali Goudarztalejerdi, Saeid Karimi, Saber Esmaeili, Bruno B Chomel, Alireza Sazmand
Dromedary camels ( Camelus dromedarius ) play a major economic role in many countries in Africa and Asia. Although they are resistant to harsh environmental conditions, they are susceptible to a wide range of zoonotic agents. This study aimed to provide an overview on the prevalence of selected zoonotic pathogens in blood and tissues of camels in central Iran. Blood, liver, portal lymph node, and brain were collected from 100 apparently healthy camels at a slaughterhouse in Qom city to assess the presence of DNA of Brucella spp...
June 2021: Yale Journal of Biology and Medicine
https://read.qxmd.com/read/34208761/whole-genome-sequencing-for-tracing-the-genetic-diversity-of-brucella-abortus-and-brucella-melitensis-isolated-from-livestock-in-egypt
#12
Aman Ullah Khan, Falk Melzer, Ashraf E Sayour, Waleed S Shell, Jörg Linde, Mostafa Abdel-Glil, Sherif A G E El-Soally, Mandy C Elschner, Hossam E M Sayour, Eman Shawkat Ramadan, Shereen Aziz Mohamed, Ashraf Hendam, Rania I Ismail, Lubna F Farahat, Uwe Roesler, Heinrich Neubauer, Hosny El-Adawy
Brucellosis is a highly contagious zoonosis that occurs worldwide. Whole-genome sequencing (WGS) has become a widely accepted molecular typing method for outbreak tracing and genomic epidemiology of brucellosis. Twenty-nine Brucella spp. (eight B. abortus biovar 1 and 21 B. melitensis biovar 3) were isolated from lymph nodes, milk, and fetal abomasal contents of infected cattle, buffaloes, sheep, and goats originating from nine districts in Egypt. The isolates were identified by microbiological methods and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
June 16, 2021: Pathogens
https://read.qxmd.com/read/34192652/spatiotemporal-distribution-and-temporal-trends-of-brucellosis-and-tuberculosis-in-water-buffalo-bubalus-bubalis-in-brazil
#13
David Germano Gonçalves Schwarz, Pedro Ferreira de Sousa Júnior, Luciana Saraiva da Silva, Richard Costa Polveiro, Juliana Ferreira de Oliveira, Márcia Paula Oliveira Faria, Glenda Lídice de Oliveira Cortez Marinho, Raylson Pereira de Oliveira, Maria Aparecida Scatamburlo Moreira
The buffalo herds in Brazil have been an alternative for increasing the economy in different biomes. For this reason, knowledge of the spatial distribution of diseases of mandatory notification in buffalo herds, such as brucellosis and tuberculosis, is essential to guarantee the quality of exported animal products, as well as assist in strategies of national control and eradication programs. In the present study, we aimed to evaluate the spatiotemporal distribution and temporal trends of brucellosis and tuberculosis in buffalo in Brazilian states between 2012-2019...
June 24, 2021: Preventive Veterinary Medicine
https://read.qxmd.com/read/34169616/galectin-3-regulates-proinflammatory-cytokine-function-and-favors-brucella-abortus-chronic-replication-in-macrophages-and-mice
#14
Fernanda L Tana, Erika S Guimarães, Daiane M Cerqueira, Priscila C Campos, Marco Túlio R Gomes, Fábio V Marinho, Sergio C Oliveira
In this study, we provide evidence that Galectin-3 (Gal-3) plays an important role in Brucella abortus infection. Our results showed increased Gal-3 expression and secretion in B. abortus infected macrophages and mice. Additionally, our findings indicate that Gal-3 is dispensable for BCV disruption, inflammasome activation and pyroptosis. On the other hand, we observed that Brucella-induced Gal-3 expression is crucial for induction of molecules associated to type I IFN signaling pathway, such as IFN-β, CXCL10 and guanylate-binding proteins (GBPs)...
June 24, 2021: Cellular Microbiology
https://read.qxmd.com/read/34163479/evaluation-and-differential-diagnosis-of-a-genetic-marked-brucella-vaccine-a19%C3%AE-virb12-for-cattle
#15
Jianghua Yang, Chuanyu He, Huan Zhang, Mengzhi Liu, Hailong Zhao, Lisong Ren, Dongling Wu, Fangyuan Du, Baoshan Liu, Xiaohu Han, Sun He, Zeliang Chen
Brucella abortus is an important zoonotic pathogen that causes severe economic loss to husbandry and poses a threat to human health. The B. abortus A19 live vaccine has been extensively used to prevent bovine brucellosis in China. However, it is difficult to distinguish the serological response induced by A19 from that induced by natural infection. In this study, a novel genetically marked vaccine, A19ΔvirB12, was generated and evaluated. The results indicated that A19ΔvirB12 was able to provide effective protection against B...
2021: Frontiers in Immunology
https://read.qxmd.com/read/34156660/correction-to-survival-of-brucella-abortus-s19-and-other-brucella-spp-in-the-presence-of-oxidative-stress-and-within-macrophages
#16
Jens Jacob, Antje Finke, Martin Mielke
No abstract text is available yet for this article.
June 22, 2021: Folia Microbiologica
https://read.qxmd.com/read/34151761/enhanced-antibacterial-activity-of-doxycycline-and-rifampicin-combination-loaded-in-nanoparticles-against-intracellular-brucella-abortus
#17
Shilpa Dawre, Padma V Devarajan, Abdul Samad
BACKGROUND: Brucellosis is a zoonotic disease and prevalent in livestock animals. The bacteria reside inside the macrophage cells of host. The WHO endorsed the combination treatment therapy for brucellosis as compared to monotherapy to avoid relapse and resistance. Therefore, we developed nanoparticles incorporating doxycycline and rifampicin in combination. OBJECTIVE: The aim of the study is to develop polymeric nanoparticles incorporating doxycycline as well as rifampicin and investigate the antibacterial activity of nanoparticles in U937 human macrophage cells infected with B...
June 9, 2021: Current Drug Delivery
https://read.qxmd.com/read/34132177/in-vitro-evaluation-of-immunogenicity-of-recombinant-omp25-protein-obtained-from-endemic-brucella-abortus-biovar-3-as-vaccine-candidate-molecule-against-animal-brucellosis
#18
Tuğba Atabey, Tayfun Acar, Serap Derman, Emel Ordu, Ayşegül Erdemir, Pakize Neslihan Taşlı, Günseli Kurt Gür, Fikrettin Şahin, Medine Güllüce, Tülin Arasoğlu
BACKGROUND: Brucellosis is a zoonotic disease that causes serious economic losses due to factors such as miscarriages and decreased milk yield in animals. Existing live vaccines have some disadvantages, so effective vaccines need to be developed with new technological approaches. OBJECTIVES: The primary objectives of this study were the expression and purification of recombinant Omp25 fusion protein from B. abortus and the evaluation of the effect of the Omp25 protein on cell viability and inflammatory response...
June 14, 2021: Protein and Peptide Letters
https://read.qxmd.com/read/34112742/structure-and-function-of-the-autolysin-saga-in-the-type-iv-secretion-system-of-brucella-abortus
#19
Yongseong Hyun, Yeongjin Baek, Chanyoung Lee, Nayeon Ki, Jinsook Ahn, Sangryeol Ryu, Nam-Chul Ha
A recent genetic study with Brucella abortus revealed the secretion activator gene A (SagA) as an autolysin component creating pores in the peptidoglycan (PGN) layer for the type IV secretion system (T4SS) and peptidoglycan hydrolase inhibitor A (PhiA) as an inhibitor of SagA. In this study, we determined the crystal structures of both SagA and PhiA. Notably, the SagA structure contained a PGN fragment in a space between the N- and C-terminal domains, showing the substrate-dependent hinge motion of the domains...
June 11, 2021: Molecules and Cells
https://read.qxmd.com/read/34078437/screening-of-potential-vaccine-candidates-against-pathogenic-brucella-spp-using-compositive-reverse-vaccinology
#20
Xiaodong Zai, Ying Yin, Fengyu Guo, Qiaoling Yang, Ruihua Li, Yaohui Li, Jun Zhang, Junjie Xu, Wei Chen
Brucella spp. are Gram-negative, facultative intracellular bacteria that cause brucellosis in humans and various animals. The threat of brucellosis has increased, yet currently available live attenuated vaccines still have drawbacks. Therefore, subunit vaccines, produced using protein antigens and having the advantage of being safe, cost-effective and efficacious, are urgently needed. In this study, we used core proteome analysis and a compositive RV methodology to screen potential broad-spectrum antigens against 213 pathogenic strains of Brucella spp...
June 2, 2021: Veterinary Research
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