Use the keywords feature with a free QxMD account.
Use Read by QxMD to access full text via your institution or open access sources.
Read also provides personalized recommendations to keep you up to date in your field.
Existing User
Sign InNew to Read
Sign Up#21
JOURNAL ARTICLE
Fengbo Zhao, Weili Yu, Jingyan Hu, Yi Xia, YuXuan Li, Siqi Liu, Aifen Liu, Chengniu Wang, Hong Zhang, Lei Zhang, Jianwu Shi
BACKGROUND: Hypoxia disrupts glucose metabolism in hepatocellular carcinoma (HCC). Transient receptor potential cation channel, subfamily M, member 7 (TRPM7) plays an ontogenetic role. Thus, we aimed to explore the regulation of TRPM7 by hypoxia-induced factor (HIF) and its underlying mechanisms in HCC. METHODS: hypoxia was induced in multiple HCC cells using 1% O2 or CoCl2 treatment, and subsequently blocked using siRNAs targeting HIF-1α or HIF-2α as well as a HIF-1α protein synthesis inhibitor...
April 25, 2024: European Journal of Pharmacology
#22
JOURNAL ARTICLE
Nobuki Kazuta, Shohei Tsuchihashi, Hiroyuki Watanabe, Masahiro Ono
OBJECTIVE: The marked success of prostate-specific membrane antigen (PSMA)-targeting radioligands with albumin binder (ALB) is attributed to the improvement of blood retention and tumor accumulation. [111 In]In-PNT-DA1, our PSMA-targeting radioligand with ALB, also achieved improved tumor accumulation due to its prolonged blood retention. Although the advantage of ALBs is related to their reversible binding to albumin, the relationship between albumin-binding and tumor accumulation of PSMA-targeting radioligands remains unclear because of the lack of information about radioligands with stronger albumin-binding than ALBs...
April 27, 2024: Annals of Nuclear Medicine
#23
JOURNAL ARTICLE
Konstantin Simon, Philipp Reichardt, Andreas Luch, Alexander Roloff, Katherina Siewert, Franziska Riedel
BACKGROUND: Contrary to Ni2+ - and Co2+ -induced allergic contact dermatitis (ACD), reactions against Pd2+ are rare. However, Pd2+ activates a larger T cell fraction in vitro, suggesting an inefficient skin penetration. OBJECTIVES: This study compares Ni2+ , Co2+ and Pd2+ skin penetration from commonly used diagnostic patch test preparations (PTPs) and aqueous metal salt solutions. METHODS: Using Franz diffusion cell assays, we applied the metals in PTPs (5% NiSO4 , 1% CoCl2 , 2% PdCl2 and 3% Na2 PdCl4 ) and in solution to pigskin for 48 h, thereby mirroring the time frame of a patch test...
April 27, 2024: Contact Dermatitis
#24
JOURNAL ARTICLE
Murat Alpaslan, Elodie Fastré, Sandrine Mestre, Arie van Haeringen, Gabriela M Repetto, Kathelijn Keymolen, Laurence M Boon, Florence Belva, Guido Giacalone, Nicole Revencu, Yves Sznajer, Katie Riches, Vaughan Keeley, Sahar Mansour, Kristiana Gordon, Silvia Martin-Almedina, Sara Dobbins, Pia Ostergaard, Isabelle Quere, Pascal Brouillard, Miikka Vikkula
Developmental and functional defects in the lymphatic system are responsible for primary lymphoedema (PL). PL is a chronic debilitating disease caused by increased accumulation of interstitial fluid, predisposing to inflammation, infections and fibrosis. There is no cure, only symptomatic treatment is available. Thirty-two genes or loci have been linked to PL, and another 22 are suggested, including Hepatocyte Growth Factor (HGF). We searched for HGF variants in 770 index patients from the Brussels PL cohort...
April 26, 2024: Human Molecular Genetics
#25
JOURNAL ARTICLE
Wai-Sing Chan, Christy Wing-Yiu Ho, Tsz-Ching Chan, Jeffrey Hung, Man-Yan To, Sau-Man Leung, Ka-Chun Lai, Ching-Yan Wong, Chin-Pang Leung, Chun-Hang Au, Thomas Shek-Kong Wan, Jonpaul Sze-Tsing Zee, Edmond Shiu-Kwan Ma, Bone Siu-Fai Tang
The BIOFIRE SPOTFIRE Respiratory (R) Panel is a novel, in vitro diagnostic PCR assay with 15 pathogen targets. The runtime is about 15 min which is the shortest among similar panels in the market. We evaluated the performance of the SPOTFIRE R Panel with 151 specimens, including 133 collected from the upper respiratory tract (URT), 13 from the lower respiratory tract (LRT) and 5 external quality assessment program (EQAP) samples. The respiratory specimens were enrolled throughout the first two post-COVID-19 influenza seasons in Hong Kong (March to December 2023)...
April 13, 2024: Viruses
#26
JOURNAL ARTICLE
Florencio J D M Machado, Juan Marta-Enguita, Susan U Gómez, Jose A Rodriguez, José Antonio Páramo-Fernández, María Herrera, Beatriz Zandio, Nuria Aymerich, Roberto Muñoz, Rebeca Bermejo, Javier Marta-Moreno, Begoña López, Arantxa González, Carmen Roncal, Josune Orbe
Accurate etiologic diagnosis provides an appropriate secondary prevention and better prognosis in ischemic stroke (IS) patients; still, 45% of IS are cryptogenic, urging us to enhance diagnostic precision. We have studied the transcriptomic content of plasma extracellular vesicles (EVs) ( n = 21) to identify potential biomarkers of IS etiologies. The proteins encoded by the selected genes were measured in the sera of IS patients ( n = 114) and in hypertensive patients with ( n = 78) and without atrial fibrillation (AF) ( n = 20)...
April 16, 2024: International Journal of Molecular Sciences
#27
JOURNAL ARTICLE
Lee Armstrong, Colin E Willoughby, Declan J McKenna
Aberrant expression of miR-145-5p has been observed in prostate cancer where is has been suggested to play a tumor suppressor role. In other cancers, miR-145-5p acts as an inhibitor of epithelial-to-mesenchymal transition (EMT), a key molecular process for tumor progression. However, the interaction between miR-145-5p and EMT remains to be elucidated in prostate cancer. In this paper the link between miR-145-5p and EMT in prostate cancer was investigated using a combination of in silico and in vitro analyses...
April 12, 2024: International Journal of Molecular Sciences
#28
JOURNAL ARTICLE
Christian D Taeger, Clemens Muehle, Philipp Kruppa, Lukas Prantl, Niklas Biermann
Background : Negative pressure wound therapy (NPWT) is an intensely investigated topic, but its mechanism of action accounts for one of the least understood ones in the area of wound healing. Apart from a misleading nomenclature, by far the most used diagnostic tool to investigate NPWT, the laser Doppler, also has its weaknesses regarding the detection of changes in blood flow and velocity. The aim of the present study is to explain laser Doppler readings within the context of NPWT influence. Methods : The cutaneous microcirculation beneath an NPWT system of 10 healthy volunteers was assessed using two different laser Dopplers (O2C/Rad-97® )...
April 18, 2024: Journal of Clinical Medicine
#29
REVIEW
Michał Jakub Braczkowski, Klaudia Maria Kufel, Julia Kulińska, Daniel Łukasz Czyż, Aleksander Dittmann, Michał Wiertelak, Marcin Sławomir Młodzik, Ryszard Braczkowski, Dariusz Soszyński
The aim of this study is to review and analyze the pleiotropic effects of TGF-β in physiological and pathological conditions of the liver, with particular emphasis on its role in immune suppression, wound healing, regulation of cell growth and differentiation, and liver cell apoptosis. A literature review was conducted, including 52 studies, comprising review articles, in vitro and in vivo studies, and meta-analyses. Only studies published in peer-reviewed scientific journals were included in the analysis...
April 22, 2024: Biomedicines
#30
JOURNAL ARTICLE
DNA Methylation Profiles Are Stable in H3 K27M-Mutant Diffuse Midline Glioma Neurosphere Cell Lines.
Matthew J Schniederjan, Cahil Potnis, Varshini Vasudevaraja, Catherine D Moser, Bethany Watson, Matija Snuderl, Tobey MacDonald, Beverly B Rogers
Diffuse midline gliomas are among the deadliest human cancers and have had little progress in treatment in the last 50 years. Cell cultures of these tumors have been developed recently, but the degree to which such cultures retain the characteristics of the source tumors is unknown. DNA methylation profiling offers a powerful tool to look at genome-wide epigenetic changes that are biologically meaningful and can help assess the similarity of cultured tumor cells to their in vivo progenitors. Paraffinized diagnostic tissue from three diffuse intrinsic pontine gliomas with H3 K27M mutations was compared with subsequent passages of neurosphere cell cultures from those tumors...
April 20, 2024: Children
#31
JOURNAL ARTICLE
Jingwen Wu, Wenya Shao, Xu Liu, Fuli Zheng, Yaping Wang, Ping Cai, Zhenkun Guo, Hong Hu, Guangxia Yu, Jianhui Guo, Linlin Yao, Siying Wu, Huangyuan Li
The exact mechanisms underlying the initiation and exacerbation of Parkinson's disease (PD) by paraquat remain unclear. We have revealed that exosomes mediate neurotoxicity induced by low dose paraquat exposure by transmitting intercellular signaling. Exposure to 40 μM paraquat promoted exosome release from mouse microglia cells (BV2) in vitro. Paraquat exposure at 100 μM caused degeneration of mouse dopaminergic MN9D cells and inhibited microglia exosome uptake by fluorescently labeling exosomes...
April 24, 2024: Environmental Pollution
#32
JOURNAL ARTICLE
Jing Huang, Jing Shi, Xiuyu Zhang, Feng Tian, Juan Huang, Qing Zhao, Ningyi Wan, Lijun Zhang, Ying Hu, Pu Li
BACKGROUND: The role of Gasdermin D (GSDMD) in bloodstream infection (BSI) diagnosis is unknown. METHODS: Serum GSDMD levels were measured in BSI patients. Endothelial cells and PBMCs were isolated, infected with bacteria/fungi, and intracellular/extracellular GSDMD concentrations were measured. An animal model was established to investigate the association between serum GSDMD levels and BSI incidence/progression. RESULTS: ROC curve analysis indicated that GSDMD could be a potential early diagnostic biomarker for BSI (AUC = 0...
April 26, 2024: Journal of Infectious Diseases
#33
JOURNAL ARTICLE
Sashary Ramos, Jennifer C Lee
Neurodegenerative diseases, such as Alzheimer's and Parkinson's, share a common pathological feature of amyloid structure accumulation. However, the structure-function relationship between these well-ordered, β-sheet-rich, filamentous protein deposits and disease etiology remains to be defined. Recently, an emerging hypothesis has linked phase separation, a process involved in the formation of protein condensates, to amyloid formation, suggesting that liquid protein droplets serve as loci for amyloid initiation...
April 26, 2024: Biochemical Society Transactions
#34
JOURNAL ARTICLE
Nikolaos Tachatos, Jan Folkard Willms, Michael Sebastian Gerlt, Kiran Kuruvithadam, Michael Hugelshofer, Kevin Akeret, Jeremy Deuel, Emanuela Keller, Marianne Schmid Daners
Delayed cerebral ischemia (DCI) occurs in up to one third of patients suffering from aneurysmal subarachnoid hemorrhage (aSAH). Untreated, it leads to secondary cerebral infarctions and is frequently associated with death or severe disability. After aneurysm rupture, erythrocytes in the subarachnoid space lyse and liberate free hemoglobin (Hb), a key driver for the development of DCI. Hemoglobin in the cerebrospinal fluid (CSF-Hb) can be analyzed through a two-step procedure of centrifugation to exclude intact erythrocytes and subsequent spectrophotometric quantification...
2024: Frontiers in medical technology
#35
JOURNAL ARTICLE
Huiping Tao, Lihan Shen
Sepsis is a life-threatening organ dysfunction caused by an unregulated host response to infection. It is an important clinical problem in acute and critical care. In recent years, with the increasing research on the epidemiology, and pathogenesis, diagnostic and therapeutic strategies of sepsis, great progress has been made in clinical practice, but there is still a lack of specific and effective treatment plans. Curcuma longa, a leafy plant of the ginger family, which is a common and safe compound, has multiple pharmacological actions, including, but not limited to, scavenging of oxygen free radicals, attenuation of inflammatory response, and anti-fibrotic effects...
March 25, 2024: Shock
#36
JOURNAL ARTICLE
Tianxiong Ji, Chunfang Zan, Lina Li, Jianbo Cao, Yao Su, Hongliang Wang, Zhifang Wu, Min-Fu Yang, Kefei Dou, Sijin Li
AIM: Atherosclerosis remains the pathological basis of myocardial infarction and ischemic stroke. Early and accurate identification of plauqes is crucial to improve clinical outcomes of atherosclerosis patients. Our study aims to evaluate the potential value of fibroblast activation protein inhibitor (FAPI)-04 PET/CT in identifying plaques via a preclinical rabbit model of atherosclerosis. METHODS: New Zealand white rabbits were fed high-fat diet (HFD), and randomly divided into the model group injured by the balloon, and the sham group only with incisions...
April 25, 2024: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
#37
JOURNAL ARTICLE
David Frisvold, Meeshanthini Dogan, Timur Dogan, Khullani Abdullahi, Tyler Koep, Robert Philibert
INTRODUCTION: The cost of secondary prevention of coronary heart disease (CHD) is continuing to increase, with a substantial portion of this acceleration being driven by the expense of confirmatory diagnostic testing. Conceivably, newly developed precision epigenetic technologies could drive down these costs. However, at the current time, their impact on overall expense for CHD care is poorly understood. We hypothesized that the use of a newly developed, highly sensitive, and specific epigenetic test, PrecisionCHD, could decrease the costs of secondary prevention...
April 25, 2024: Advances in Therapy
#38
JOURNAL ARTICLE
Anjali Mundkur, Padavagodu Shivananda Roopa, Pratap Kumar Narayan, Akhila Vasudeva, Gazal Jain, Prashanth K Adiga
Gestational diabetes mellitus is a common medical disorder of pregnancy. Diabetic ketoacidosis is a complication that may affect both maternal and perinatal wellbeing adversely. It is rare, most often involving women with type 1 or type 2 diabetes, but occasionally can be seen in gestational diabetes mellitus. Here are two cases of ketoacidosis seemingly triggered by glucose ingestion for the oral glucose tolerance test in previously normoglycemic women, posing a diagnostic and therapeutic challenge. Prevention of such complications must be considered when treating high-risk pregnant women> 40 years of age, pregnant as a result of assisted reproductive techniques...
March 2024: Obstetric Medicine
#39
JOURNAL ARTICLE
Yuta Ito, Amira Marouf, Yasunori Kogure, Junji Koya, Raphaël Liévin, Julie Bruneau, Mariko Tabata, Yuki Saito, Sumito Shingaki, Mitsuhiro Yuasa, Kentaro Yamaguchi, Koichi Murakami, Robert Weil, Manon Vavasseur, Guillaume P Andrieu, Mehdi Latiri, Layla Veleanu, Michaël Dussiot, Isabelle André, Akshay Joshi, Chantal Lagresle-Peyrou, Aude Magerus, Sammara Chaubard, David Lavergne, Emmanuel Bachy, Erika Brunet, Virginie Fataccioli, Chantal Brouzes, Camille Laurent, Laurence De Leval, Alexandra Traverse-Glehen, Céline Bossard, Marie-Cécile Parrens, Véronique Meignin, Laure Philippe, Julien Rossignol, Felipe Suarez, Jean-Marie Michot, Olivier Tournilhac, Gandhi Damaj, François Lemonnier, Christine Bôle-Feysot, Patrick Nitschké, Bruno Tesson, Cécile Laurent, Thierry Molina, Vahid Asnafi, Yosaku Watatani, Kenichi Chiba, Ai Okada, Yuichi Shiraishi, Sachiko Tsukita, Koji Izutsu, Hiroaki Miyoshi, Koichi Ohshima, Seiji Sakata, Akito Dobashi, Kengo Takeuchi, Masashi Sanada, Philippe Gaulard, Arnaud Jaccard, Seishi Ogawa, Olivier Hermine, Keisuke Kataoka, Lucile Couronné
Extranodal NK/T-cell lymphoma (ENKTCL) is an Epstein-Barr virus (EBV)-related neoplasm with male dominance and a poor prognosis. A better understanding of the genetic alterations and their functional roles in ENKTCL could help improve patient stratification and treatments. Here, we performed comprehensive genetic analysis of 177 ENKTCL cases to delineate the landscape of mutations, copy number alterations (CNAs), and structural variations, identifying 34 driver genes including six previously unappreciated ones, namely HLA-B, HLA-C, ROBO1, CD58, POT1, and MAP2K1...
April 24, 2024: Cancer Research
#40
JOURNAL ARTICLE
Kazuhisa Ouhara, Tasuku Takemura, Yuri Taniguchi, Ryousuke Fujimori, Tetsuya Tamura, Yuki Akane, Shinji Matsuda, Yuta Hamamoto, Tomoaki Shintani, Mikihito Kajiya, Syuichi Munenaga, Tomoyuki Iwata, Tsuyoshi Fujita, Noriyoshi Mizuno
OBJECTIVE: To investigate the production of leucine-rich α-2-glycoprotein-1 (LRG1) in periodontitis patients and its effectiveness as a new diagnostic marker for periodontitis. SUBJECTS AND METHODS: In vitro experiments were conducted to analyze LRG1 mRNA expression in human gingival epithelial cells and fibroblasts via quantitative real-time PCR. In vivo experiments were conducted to analyze LRG1 localization in periodontitis patients. The correlation between the serum LRG1 levels and alveolar bone resorption in the mouse periodontitis model was also investigated...
April 24, 2024: Oral Diseases
Make the most of Read.
Download the mobile app.
Download the mobile app.
Fetching more papers... 
