Ansen H P Burr, Junyi Ji, Kadir Ozler, Heather L Mentrup, Onur Eskiocak, Brian Yueh, Rachel Cumberland, Ashley V Menk, Natalie Rittenhouse, Chris W Marshall, Pailin Chiaranunt, Xiaoyi Zhang, Lauren Mullinax, Abigail Overacre-Delgoffe, Vaughn S Cooper, Amanda C Poholek, Greg M Delgoffe, Kevin P Mollen, Semir Beyaz, Timothy W Hand
BACKGROUND & AIMS: The colonic epithelium requires continuous renewal by crypt resident intestinal stem cells (ISCs) and transit-amplifying (TA) cells to maintain barrier integrity, especially after inflammatory damage. The diet of high-income countries contains increasing amounts of sugar, such as sucrose. ISCs and TA cells are sensitive to dietary metabolites, but whether excess sugar affects their function directly is unknown. METHODS: Here, we used a combination of 3-dimensional colonoids and a mouse model of colon damage/repair (dextran sodium sulfate colitis) to show the direct effect of sugar on the transcriptional, metabolic, and regenerative functions of crypt ISCs and TA cells...
May 11, 2023: Cellular and Molecular Gastroenterology and Hepatology