keyword
https://read.qxmd.com/read/34325196/metabolic-profiling-of-nanosilver-toxicity-in-the-gills-of-common-carp
#1
Qin-Qin Li, Qian-Qian Xiang, Li-Hong Lian, Zhi-Ying Chen, Xia Luo, Cheng-Zhi Ding, Li-Qiang Chen
Studies have shown silver nanoparticles (AgNPs) exposure can result in a series of toxic effects in fish gills. However, it is still unclear how AgNPs affect metabolite expression and their related molecular metabolic pathways in fish gills. In this study, we employed untargeted metabolomics to study the effects of AgNPs and silver supernatant ions on fish gill metabolites. The results showed that AgNPs can induce significant changes in 96 differentially expressed metabolites, which mainly affect amino acid metabolism and energy metabolism in fish gills...
July 26, 2021: Ecotoxicology and Environmental Safety
https://read.qxmd.com/read/34322102/the-abai-abar-quorum-sensing-system-effects-on-pathogenicity-in-acinetobacter-baumannii
#2
Xiaoyu Sun, Zhaohui Ni, Jie Tang, Yue Ding, Xinlei Wang, Fan Li
Acinetobacter baumannii is a Gram-negative pathogen that has emerged as one of the most troublesome pathogens for healthcare institutions globally. Bacterial quorum sensing (QS) is a process of cell-to-cell communication that relies on the production, secretion, and detection of autoinducer (AI) signals to share information about cell density and regulate gene expression accordingly. The molecular and genetic bases of A. baumannii virulence remains poorly understood. Therefore, the contribution of the abaI / abaR QS system to growth characteristics, morphology, biofilm formation, resistance, motility, and virulence of A...
2021: Frontiers in Microbiology
https://read.qxmd.com/read/34321676/natural-deep-eutectic-systems-for-nature-inspired-cryopreservation-of-cells
#3
Kathlyn Hornberger, Rui Li, Ana Rita C Duarte, Allison Hubel
Natural deep eutectic systems (NADES) are emerging as potential cryoprotective agents (CPA) for cell preservation. In this investigation, we develop an optimized CPA formulation using trehalose-glycerol NADES (T:G) diluted in Normosol-R and supplemented with isoleucine. Differential scanning calorimetry (DSC) is used to define the thermophysical properties of NADES-based solutions, and Raman spectroscopy is used to characterize the effect of NADES on ice formation and hydrogen bonding. Jurkat cells are cryopreserved in each solution, and post-thaw cell recovery, apoptosis, and growth are quantified...
February 2021: AIChE Journal
https://read.qxmd.com/read/34318701/increased-plasma-and-milk-short-chain-acylcarnitines-concentrations-reflect-systemic-lps-response-in-mid-lactation-dairy-cows
#4
Wei Xu, Sandra Grindler, Sven Dänicke, Jana Frahm, Akos Kenez, Korinna Huber
Lipopolysaccharides (LPS) challenge the metabolic integrity of high yielding dairy cows, activating the immune system and altering energy metabolism. Fatty acid oxidation, a major energy gaining pathway, can be improved by supplementary carnitine, facilitating the transport of fatty acids into mitochondria. The metabolic response to LPS challenge could alter both the plasma and the milk metabolome. Plasma and milk samples collected from cows treated with (n=27) or without (n=27) dietary carnitine, before and after intravenous administration of LPS, were subjected to a targeted metabolomics analysis...
July 28, 2021: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://read.qxmd.com/read/34316211/assessment-of-quality-and-safety-of-meats-from-various-animal-species-in-the-shuchinsk-burabay-resort-zone-kazakhstan
#5
B S Maikanov, G T Ismagulova, L T Auteleyeva, Zh O Kemeshov, D K Zhanabayeva
Aim: This study aimed to determine the food safety and protein adequacy of meats from various animals in the Shuchinsk-Burabay resort zone. Materials and Methods: Samples of meat were collected from markets "Zhomart" and "Kausar." Two hundred and ninety-eight samples of meat were obtained: beef - 166, horse - 42, pork - 67, mutton - 8, and poultry - 15. From each carcass, part of the carcass, point samples were taken to form an average sample and conduct research...
June 2021: Veterinary World
https://read.qxmd.com/read/34316185/effect-of-dietary-tannins-on-the-performance-lymphoid-organ-weight-and-amino-acid-ileal-digestibility-of-broiler-chickens-a-meta-analysis
#6
Cecep Hidayat, Agung Irawan, Anuraga Jayanegara, Muhammad Miftakhus Sholikin, Tri Rachmanto Prihambodo, Yulianri Rizki Yanza, Elizabeth Wina, Sadarman Sadarman, Rantan Krisnan, Isbandi Isbandi
Background and Aim: Tannins are functional secondary metabolites that may provide benefits to ruminants. However, to date, their effects on broiler chickens remain inconclusive. This study aimed to evaluate the effectiveness of dietary tannin levels on the performance, body organs, and amino acid (AA) digestibility of broiler chickens using a meta-analysis. Materials and Methods: After verification and evaluation, a total of 22 articles were included in the present study...
June 2021: Veterinary World
https://read.qxmd.com/read/34315916/comparative-assessment-of-amino-acids-composition-in-two-types-of-marine-fish-silage
#7
Mukund Gauthankar, Rakhee Khandeparker, Mamatha S Shivaramu, Komal Salkar, Rayadurga Anantha Sreepada, Mandar Paingankar
Fish silage is a brown liquefied product achieved by the action of enzymes when finely grounded whole/parts of either single or mixed fish types are subjected to acidification. This study made a comparative assessment of biochemical and nutritive properties, especially the amino acid composition in supernatant phase of formic acid silages prepared from two fish types, Indian mackerel (Rastrelliger kanagurta) and false travely (Lactarius lactarius) representing fat fish (FF, fat content > 5%) and lean fish (LF, fat content < 5%), respectively during 35 days of fermentation (DoF)...
July 27, 2021: Scientific Reports
https://read.qxmd.com/read/34299600/fluorimetric-analysis-of-five-amino-acids-in-chocolate-development-and-validation
#8
Maria S Synaridou, Vasilis Tsamis, Georgia Sidiropoulou, Constantinos K Zacharis, Irene Panderi, Catherine K Markopoulou
Amino acids present ergogenic action, helping to increase, protect, and restore the muscular system of young athletes. Moreover, the encapsulation of five relevant amino acids in chocolate pellet form will appeal to them, facilitating their daily consumption. A reliable HPLC fluorimetric method was developed to detect and quantitatively determine L-Leucine, L-Isoleucine, L-Histidine, L-Valine, and β-Alanine in chocolate using aniline as an internal standard. Experimental design methodology was used to investigate and optimize the clean-up procedure of the samples...
July 16, 2021: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/34299216/integration-of-transcriptome-and-metabolome-reveals-the-genes-and-metabolites-involved-in-bifidobacterium-bifidum-biofilm-formation
#9
Zongmin Liu, Lingzhi Li, Zhifeng Fang, Yuankun Lee, Jianxin Zhao, Hao Zhang, Wei Chen, Haitao Li, Wenwei Lu
Bifidobacterium bifidum strains, an important component of probiotic foods, can form biofilms on abiotic surfaces, leading to increased self-resistance. However, little is known about the molecular mechanism of B. bifidum biofilm formation. A time series transcriptome sequencing and untargeted metabolomics analysis of both B. bifidum biofilm and planktonic cells was performed to identify key genes and metabolites involved in biofilm formation. Two hundred thirty-five nonredundant differentially expressed genes (DEGs) (including vanY , pstS , degP , groS , infC , groL , yajC , tadB and sigA ) and 219 nonredundant differentially expressed metabolites (including L-threonine, L-cystine, L-tyrosine, ascorbic acid, niacinamide, butyric acid and sphinganine) were identified...
July 15, 2021: International Journal of Molecular Sciences
https://read.qxmd.com/read/34297429/mitochondrial-damage-in-renal-epithelial-cells-is-potentiated-by-protein-exposure-in-propionic-aciduria
#10
Anke Schumann, Véronique Belche, Kristin Schaller, Sarah C Grünert, Andres Kaech, Matthias R Baumgartner, Stefan Kölker, Luciana Hannibal, Ute Spiekerkoetter
Propionic aciduria (PA) is caused by deficiency of the mitochondrial enzyme propionyl-CoA carboxylase (PCC). Due to inefficient propionate catabolism patients are endangered by life-threatening ketoacidotic crisis. Protein and amino acid restriction are major therapeutic pillars. However, long-term complications like neurological deterioration and cardiac abnormalities cannot be prevented. Chronic kidney disease (CKD), which is a well-known characteristic of methylmalonic aciduria two enzymatic steps down-stream from PCC, has been recognized as a novel late-onset complication in PA...
July 23, 2021: Journal of Inherited Metabolic Disease
https://read.qxmd.com/read/34288399/maple-syrup-urine-disease-characteristics-of-diagnosis-and-treatment-in-45-patients-in-chile
#11
María Fernanda Medina, Gabriela Castro, Felipe Falcon, Juan Francisco Cabello, Víctor Faundes, Diana Ruffato, María Florencia Salazar, Carolina Arias, Felipe Peñaloza, Alicia De La Parra, Verónica Cornejo
Maple urine syrup disease (MSUD) is an autosomal recessive disorder characterized by deficient activity of the branched-chain alpha ketoacid dehydrogenase (BCKAD) enzymatic complex due to biallelic variants in the alpha (BCKDHA) or beta (BCKDHB) subunits or the acyltransferase component (DBT). Treatment consists in leucine (LEU), isoleucine (ILE), and valine (VAL) (branched-chain amino acids) dietary restriction and strict metabolic control. to determine the characteristics of the Chilean cohort with MSUD currently in follow-up at Instituto de Nutrición y Tecnología de los Alimentos, during the 1990-2017 period Retrospective analytical study in 45 MSUD cases...
July 21, 2021: American Journal of Medical Genetics. Part C, Seminars in Medical Genetics
https://read.qxmd.com/read/34287228/development-of-second-tier-liquid-chromatography-tandem-mass-spectrometry-analysis-for-expanded-newborn-screening-in-japan
#12
Yosuke Shigematsu, Miori Yuasa, Nobuyuki Ishige, Hideki Nakajima, Go Tajima
To minimize false-positive cases in newborn screening by tandem mass spectrometry in Japan, practical second-tier liquid chromatography-tandem mass spectrometry analyses have been developed using a multimode ODS column with a single set of mobile phases and different gradient elution programs specific to the analysis of acylcarnitines, acylglycines, amino acids, and organic acids. Most analyses were performed using underivatized samples, except for analysis of methylcitric acid, and careful conditioning of the column was necessary for analyses of organic acids...
July 14, 2021: International Journal of Neonatal Screening
https://read.qxmd.com/read/34276153/screening-of-family-members-of-nonalcoholic-fatty-liver-disease-patients-can-detect-undiagnosed-nonalcoholic-fatty-liver-disease-among-them-is-there-a-genetic-link
#13
Shubham Jain, Ravi Thanage, Falguni Panchal, Pravin M Rathi, Renuka Munshi, Suhas S Udgirkar, Qais Q Contractor, Sanjay J Chandnani, Nair P Sujit, Partha Debnath, Anupam Singh
Background & aims: Nonalcoholic fatty liver disease (NAFLD) has multifactorial origin. Genetic and environmental factors lead to the biology of this complex disorder. In this study, we screened parents of cases with NAFLD and compared them with parents of cases without NAFLD to see its familial aggregation and the role of patatin-like phospholipase domain containing 3 (PNPLA3). Method: It was a cross-sectional study. Parents of probands with NAFLD and without NAFLD were screened with abdominal sonography, anthropometry, blood tests, transient elastography, and PNPLA3 polymorphism...
July 2021: Journal of Clinical and Experimental Hepatology
https://read.qxmd.com/read/34273658/investigation-on-the-stability-in-plant-metabolomics-with-a-special-focus-on-freeze-thaw-cycles-lc-ms-and-nmr-analysis-to-cassiae-semen-cassia-obtusifolia-l-seeds-as-a-case-study
#14
Biru Shi, Hui Ding, Liming Wang, Chenxi Wang, Xiaoxuan Tian, Zhifei Fu, Lihua Zhang, Lifeng Han
Metabolomics is a rapid and sensitive tool for the detection of dynamic metabolic compositions in the study of systemic metabolic consequences. However, it is also susceptible to a tiny variation of pre-analytical handling procedures. To provide reproducible results, specific knowledge on metabolites perturbance along with different freeze-thaw cycles (FTCs) is needed for further metabolomics studies. In this paper, five FTCs of germinated Cassiae Semen (CS) were chosen as a case study to investigate the influence of FTC effect based on UHPLC-Q-Orbitrap-MS and NMR technologies...
July 6, 2021: Journal of Pharmaceutical and Biomedical Analysis
https://read.qxmd.com/read/34273619/modeling-a-human-clp1-mutation-in-mouse-identifies-an-accumulation-of-tyrosine-pre-trna-fragments-causing-pontocerebellar-hypoplasia-type-10
#15
Ikuko Morisaki, Hiroshi Shiraishi, Hiroyuki Fujinami, Nobuyuki Shimizu, Takatoshi Hikida, Yuji Arai, Takashi Kobayashi, Reiko Hanada, Josef M Penninger, Minoru Fujiki, Toshikatsu Hanada
Cleavage factor polyribonucleotide kinase subunit 1 (CLP1), an RNA kinase, plays essential roles in protein complexes involved in the 3'-end formation and polyadenylation of mRNA and the tRNA splicing endonuclease complex, which is involved in precursor tRNA splicing. The mutation R140H in human CLP1 causes pontocerebellar hypoplasia type 10 (PCH10), which is characterized by microcephaly and axonal peripheral neuropathy. Previously, we reported that RNA fragments derived from isoleucine pre-tRNA introns (Ile-introns) accumulate in fibroblasts of patients with PCH10...
July 14, 2021: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/34272643/rational-development-of-stable-pyy-3-36-peptide-y-2-receptor-agonists
#16
Christian Poulsen, Marie Østergaard Pedersen, Per-Olof Wahlund, Annika Sjölander, Jens Kaalby Thomsen, Kilian W Conde-Frieboes, Johan F Paulsson, Birgitte S Wulff, Søren Østergaard
PURPOSE: The anorectic effect of PYY3-36 makes it a potential pharmacological weight loss treatment. Modifications of the endogenous peptide to obtain commercially attractive pharmacological and biophysical stability properties are examined. METHODS: Half-life extended PYY3-36 analogues were prepared and examined regarding Y2 -receptor potency as well as biophysical and stability properties. RESULTS: Deamidation of asparagine in position 18 and 29 was observed upon incubation at 37°C...
July 16, 2021: Pharmaceutical Research
https://read.qxmd.com/read/34270256/sodium-binding-interactions-with-aliphatic-amino-acids-a-guided-ion-beam-and-computational-study
#17
Hanh D M Pham, Georgia C Boles, P B Armentrout
Metal binding affinities play a vital role in medicinal, biological, and industrial applications. In particular, metal cation-amino acid (AA) interactions contribute to protein stability such that analyzing analogous prototypical interactions is important. Here, we present a full description of the interactions of sodium cations (Na+ ) and six aliphatic amino acids (AA), where AA = glycine (Gly), alanine (Ala), homoalanine (hAla), valine (Val), leucine (Leu), and isoleucine (Ile). Experimentally, these interactions are evaluated using threshold collision-induced dissociation carried out in a guided ion beam tandem mass spectrometer, allowing for the determination of the kinetic-energy-dependent behavior of Na+ -AA dissociation...
July 16, 2021: Journal of Physical Chemistry. A
https://read.qxmd.com/read/34268715/dietary-intake-of-branched-chain-amino-acids-in-relation-to-general-and-abdominal-obesity
#18
Farzaneh Asoudeh, Asma Salari-Moghaddam, Ammar Hassanzadeh Keshteli, Ahmad Esmaillzadeh, Peyman Adibi
PURPOSE: Despite the huge evidence on the link between dietary protein intake and obesity, limited studies have examined the role of individual amino acids in this regard. This study aimed to investigate the association between dietary total and individual BCAAs intake and odds of general and abdominal obesity in a large group of adults. METHODS: This cross-sectional study was conducted among 8691 adults aged 18-55 years in Isfahan, Iran. Dietary BCAAs were assessed using a validated dish-based 106-item semi-quantitative food frequency questionnaire (DS-FFQ)...
July 15, 2021: Eating and Weight Disorders: EWD
https://read.qxmd.com/read/34265484/exploring-the-metabolic-characteristics-and-pharmacokinetic-variation-of-paroxetine-in-healthy-volunteers-using-a-pharmacometabonomic-approach
#19
Zhuoling An, Xiangyi Wang, Pengfei Li, Jiuming He, Lihong Liu
The pharmacokinetic parameters of paroxetine vary between individuals, leading to differences in efficacy. The focus of our research was to predict responses to paroxetine using a pharmacometabonomic approach combining metabolic phenotypes and pharmacokinetic parameters. The pharmacokinetics of 12 healthy volunteers treated with paroxetine over two cycles was determined using high-performance liquid chromatography tandem mass spectrometry. Metabolic profiling and phenotyping were performed on the blood samples that remained after pharmacokinetic studies, using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry...
June 24, 2021: Journal of Pharmaceutical and Biomedical Analysis
https://read.qxmd.com/read/34264743/association-of-plasma-branched-chain-amino-acid-with-biomarkers-of-inflammation-and-lipid-metabolism-in-women
#20
Rikuta Hamaya, Samia Mora, Patrick R Lawler, Nancy R Cook, Paul M Ridker, Julie E Buring, I-Min Lee, JoAnn E Manson, Deirdre K Tobias
Backgrounds - Branched-chain amino acids (BCAAs; isoleucine, leucine and valine) correlate with insulin resistance and poor glucose control, which may in part explain associations between type 2 diabetes (T2D) and cardiovascular disease (CVD). However, the relationships of BCAAs with other cardiometabolic pathways, including inflammation and dyslipidemia, are unclear. We hypothesized that plasma BCAAs would correlate with multiple pathways of cardiometabolic dysfunction. Methods - We conducted a cross-sectional analysis among 19,472 participants (mean age=54...
July 15, 2021: Circulation. Genomic and Precision Medicine
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