Lisa Maria Dersch, Veronique Beckers, Detlev Rasch, Guido Melzer, Christoph Bolten, Katina Kiep, Horst Becker, Oliver Ernst Bläsing, Regine Fuchs, Thomas Ehrhardt, Christoph Wittmann
Here, we demonstrate whole-plant metabolic profiling by stable isotope labeling and combustion isotope-ratio mass spectrometry for precise quantification of assimilation, translocation, and molecular reallocation of (13)CO2 and (15)NH4NO3 The technology was applied to rice (Oryza sativa) plants at different growth stages. For adult plants, (13)CO2 labeling revealed enhanced carbon assimilation of the flag leaf from flowering to late grain-filling stage, linked to efficient translocation into the panicle. Simultaneous (13)CO2 and (15)NH4NO3 labeling with hydroponically grown seedlings was used to quantify the relative distribution of carbon and nitrogen...
May 2016: Plant Physiology