Qing Liu, Wei Ma, Ruiying Chen, Shang-Tong Li, Qifan Wang, Cai Wei, Yiguo Hong, Hai-Xi Sun, Qi Cheng, Jianjun Zhao, Jingmin Kang
Cell-specific transcriptional regulatory networks (TRNs) play vital roles in plant development and response to environmental stresses. However, traditional single-cell mono-omics techniques are unable to directly capture the relationships and dynamics between different layers of molecular information within the same cells. While advanced algorithm facilitates merging scRNA-seq and scATAC-seq datasets, accurate data integration remains a challenge, particularly when investigating cell-type-specific TRNs. By examining gene expression and chromatin accessibility simultaneously in 16,670 Arabidopsis root tip nuclei, the TRNs are reconstructed that govern root tip development under osmotic stress...
April 18, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)