Eric Vornholt, Lora E Liharska, Esther Cheng, Alice Hashemi, You Jeong Park, Kimia Ziafat, Lillian Wilkins, Hannah Silk, Lisa M Linares, Ryan C Thompson, Brendan Sullivan, Emily Moya, Girish N Nadkarni, Robert Sebra, Eric E Schadt, Brian H Kopell, Alexander W Charney, Noam D Beckmann
Single-nucleus RNA sequencing (snRNA-seq) is often used to define gene expression patterns characteristic of brain cell types as well as to identify cell type specific gene expression signatures of neurological and mental illnesses in postmortem human brains. As methods to obtain brain tissue from living individuals emerge, it is essential to characterize gene expression differences associated with tissue originating from either living or postmortem subjects using snRNA-seq, and to assess whether and how such differences may impact snRNA-seq studies of brain tissue...
May 1, 2024: medRxiv