Chong Qiu, Xiaoge Wang, Jiaojiao Zuo, Runyang Li, Cong Gao, Xiulai Chen, Jia Liu, Wanqing Wei, Jing Wu, Guipeng Hu, Wei Song, Nan Xu, Liming Liu
P-coumaric acid (p-CA), a pant metabolite with antioxidant and anti-inflammatory activity, is extensively utilized in biomedicine, food, and cosmetics industry. In this study, a synthetic pathway (PAL) for p-CA was designed, integrating three enzymes (AtPAL2, AtC4H, AtATR2) into a higher l-phenylalanine-producing strain Escherichia coli PHE05. However, the lower soluble expression and activity of AtC4H in the PAL pathway was a bottleneck for increasing p-CA titers. To overcome this limitation, the soluble expression of AtC4H was enhanced through N-terminal modifications...
April 26, 2024: Biotechnology and Bioengineering