Jimei Chi, Meng Su, Bingjie Xue, Lijun Cheng, Zewei Lian, Yang Yun, Xu Yang, Xue Wang, Hongfei Xie, Huadong Wang, Yuan Wang, Jie Du, Yanlin Song
With the demand for point-of-care testing (POCT) in cardiovascular diseases, the detection of biomarkers in trace blood samples is of great significance in emergency medicine settings. Here, we demonstrated an all-printed photonic crystal microarray for POCT of protein markers (named "P4 microarray"). The paired nanobodies were printed as probes to target the soluble suppression of tumorigenicity 2 (sST2) as a certified cardiovascular protein marker. Benefiting from photonic crystal-enhanced fluorescence and integrated microarrays, quantitative detection of sST2 is 2 orders of magnitude lower than that of a traditional fluorescent immunoassay...
March 26, 2023: ACS Sensors