Giovanni Botten, Yuannyu Zhang, Kseniia Dudnyk, Yoon Jung Kim, Xin Liu, Jacob T Sanders, Aygun Imanci, Nathalie M Droin, Hui Cao, Pranita Kaphle, Kathryn E Dickerson, Kirthi R Kumar, Mingyi Chen, Weina Chen, Eric Solary, Peter Ly, Jian Zhou, Jian Xu
Structural variants (SVs) involving enhancer hijacking can rewire chromatin topologies to cause oncogene activation in human cancers including hematologic malignancies; however, due to the lack of tools to assess their effects on gene regulation and chromatin organization, the molecular determinants for the functional output of enhancer hijacking remain poorly understood. Here, we developed a multimodal approach to integrate genome sequencing, chromosome conformation, chromatin state, and transcriptomic alteration for quantitative analysis of transcriptional effects and structural reorganization imposed by SVs in leukemic genomes...
March 22, 2023: Blood