Qixing Liang, Jingcheng Shi, Xuerong Jin, Guocheng Du, Zhen Kang
Enterokinase is a class of serine proteases that specifically recognize the cleavage DDDDK sequences. Therefore, enterokinase has been widely used as a tool enzyme in the field of biomedicine. Currently, the expression level of enterokinase in Pichia pastoris is low, which hinders related practical applications. In this study, the effects of six different signal peptides SP1, SP2, SP3, SP4, SP7 and SP8 on the secretory expression of enterokinase in Pichia pastoris were studied. Compared with α-factor, SP1 significantly increased the secretory expression of enterokinase (from 6...
August 25, 2020: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology