Vanesa García, Michela Gambino, Karl Pedersen, Sven Haugegaard, John Elmerdahl Olsen, Ana Herrero-Fresno
This study aimed to characterize in silico Enterotoxigenic E. coli F4- and F18-positive-isolates (n=90) causing swine post-weaning diarrhea, including pathogenic potential, phylogenetic relationship, antimicrobial and biocide resistance (R), prophage content and metal tolerance rates. F4-strains belonged mostly to the O149 and O6 serogroups and ST100 and ST48 MLST-types. F18-strains were mainly assigned to the O8 and O147 serogroups and ST10, ST23 and ST42. The highest rates of antimicrobial-R were found against streptomycin, sulfamethoxazole, tetracycline, trimethoprim and ampicillin...
September 18, 2020: Applied and Environmental Microbiology
Pariya Mehrbakhsh, Yalda Basharkhah, Ashraf Bahkshi, Lida Tahmasebi Nezhad Kamarposhti, Shahin Bolori
BACKGROUND: Drug- and multidrug-resistant Klebsiella pneumoniae isolates have been found worldwide. Treatment failures against carbapenems and extended-spectrum cephalosporins, the currently recommended drugs, contribute to consider K. pneumoniae infections as untreatable infections. The emergence and spread of oxacillinases (OXAs) with carbapenem-hydrolysing properties are a major concern and seriously become a public health problem worldwide. The present study was aimed to explore the blaOXA genes among clinical isolates of K...
September 17, 2020: Infectious Disorders Drug Targets
Alan Abdulla, Annemieke Dijkstra, Nicole G M Hunfeld, Henrik Endeman, Soma Bahmany, Tim M J Ewoldt, Anouk E Muller, Teun van Gelder, Diederik Gommers, Birgit C P Koch
BACKGROUND: Early and appropriate antibiotic dosing is associated with improved clinical outcomes in critically ill patients, yet target attainment remains a challenge. Traditional antibiotic dosing is not suitable in critically ill patients, since these patients undergo physiological alterations that strongly affect antibiotic exposure. For beta-lactam antibiotics, the unbound plasma concentrations above at least one to four times the minimal inhibitory concentration (MIC) for 100% of the dosing interval (100%ƒT > 1-4×MIC) have been proposed as pharmacodynamic targets (PDTs) to maximize bacteriological and clinical responses...
September 15, 2020: Critical Care: the Official Journal of the Critical Care Forum
David S Y Ong, Wieke Altorf-van der Kuil, Anne L M Vlek, Leo M Schouls, Annelot F Schoffelen
Introduction. Increased carbapenem resistance is often caused by carbapenemase production. Aim . The objective of our study was to assess which antibiotic susceptibility patterns, as tested by automated systems, are highly associated with the absence of carbapenemase production in Enterobacteriaceae isolates, and could therefore be used as a screening tool. Methodology. Routine antibiotic susceptibility testing data from 42 medical microbiology laboratories in the Netherlands in the period between January 2011 and June 2017 were obtained from the national antimicrobial resistance surveillance programme...
September 14, 2020: Journal of Medical Microbiology
Benjelloun Touimi Ghita, Laila Bennani, Sanae Berrada, Moussa Benboubker, Bahia Bennani
Escherichia coli is related to foodborne disease and outbreaks worldwide. It mainly affects persons at high risk as newborns, infants, and individuals with impaired immune system in hospitals. Multidrug-resistant E. coli is currently spreading both in community and hospital settings. Our study aims to evaluate the presence of E. coli and the incidence of its antibiotic resistance in samples obtained from various cooked and raw foods ( N  = 300), food contact surfaces ( N  = 238), and food handlers ( N  = 40) in Moroccan hospital catering service...
2020: International Journal of Microbiology
Farzad Khademi, Amirhossein Sahebkar
Streptococcus (S.) agalactiae colonizes in the female genitourinary and lower gastrointestinal tracts and is responsible for a wide range of infections in newborns, pregnant women and non-pregnant adults. Therefore, antibiotic prophylaxis and infection treatment against S. agalactiae is important. The aim of this study was to determine the prevalence of S. agalactiae antibiotic resistance in Iranian patients, especially among pregnant women. A systematic literature search was conducted in PubMed, Scopus, Google Scholar and the Scientific Information Database (SID) databases by using related keywords and without any time limitation...
September 2020: Taiwanese Journal of Obstetrics & Gynecology
Elena Bello-López, Rosa Del Carmen Rocha-Gracia, Semiramis Castro-Jaimes, Miguel Ángel Cevallos, Michelle Vargas-Cruz, Ricardo Verdugo-Yocupicio, Yolanda Sáenz, Carmen Torres, Zita Gutiérrez-Cázarez, Margarita María de la Paz Arenas-Hernández, Patricia Lozano-Zarain
OBJECTIVES: To identify Acinetobacter spp. strains from pediatric patients, determine its genetic relationship, detect antibiotic resistance genes and evaluate the participation of efflux pumps in the resistance. METHODS: Fifty-four non-duplicate, not consecutive Acinetobacter spp. isolates were collected from pediatric patients. Genetic relationship (PFGE), resistance profile (Kirby-Baüer), efflux pumps activity (MIC in the presence and absence of EPI), resistance genes (PCR and sequencing) and the plasmid profile (QuickPrep) were identified...
September 8, 2020: Journal of Global Antimicrobial Resistance
Marcelina Osińska, Aneta Nowakiewicz, Przemysław Zięba, Sebastian Gnat, Dominik Łagowski, Aleksandra Trościańczyk
In recent decades, the number of studies on the occurrence of resistant strains in wildlife animals has increased significantly, but data are still fragmentary. The aim of this study was to evaluate drug resistance of Escherichia coli strains isolated from wild carnivorous mammals, common in Poland. Selective media with antimicrobials (tetracycline, kanamycin, chloramphenicol, and cefotaxime) were used for isolation. Of 53 isolates shown to be distinct by the amplification of DNA fragments surrounding rare restriction site-fingerprinting method, 77...
September 2020: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Beatrice Ekman, Prajwal Paudel, Omkar Basnet, Ashish Kc, Johan Wrammert
BACKGROUND: Neonatal sepsis is one of the major causes of death during the first month of life and early empirical treatment with injectable antibiotics is a life-saving intervention. Adherence to World Health Organisation guidelines on first line antibiotics is crucial to mitigate the risks of increased antimicrobial resistance. The aim of this paper was to evaluate if treatment of early onset neonatal sepsis in a low-income facility setting observe current guidelines and if compliance is influenced by contextual factors...
September 10, 2020: BMC Infectious Diseases
Jeeta Sarkar, Arijit Misra, Nirmalya Banerjee
An efficient genetic transfection technique has been established using A4 strain of Agrobacterium rhizogenes for the first time in a medicinally valuable plant Solanum erianthum D. Don. The explants were randomly pricked with sterile needle, inoculated with bacterial suspension. The infected leaves were then washed and transferred to MS basal medium fortified with cefotaxime for hairy root induction. A maximum transformation efficiency of 72% has been recorded after two days of co-cultivation period. The transfer of rolA and rolB genes from the bacterium to the plant genome has been confirmed in five transformed hairy rootlines by standard Polymerase Chain Reaction technique...
September 4, 2020: Journal of Biotechnology
Ahmad Farajzadeh Sheikh, Maryam Moradi Bandbal, Morteza Saki
Owing to the scarce evidence about the multidrug-resistant (MDR) beta-lactamase-producing Shigella isolates in Iran, this study aimed to evaluate the occurrence of extended-spectrum beta-lactamases (ESBL) and AmpC β-lactamases in Shigella species collected in the southwest of Iran. This study was conducted on Shigella species isolated from stool samples of pediatric patients aged less than 15 years suffering from diarrhea. These isolates were identified by bacteriology tests, serotyping, and polymerase chain reaction (PCR)...
September 7, 2020: Molecular Biology Reports
Jin-E Lei, Qian Wang, Yue Lin, Fang Li, Chen Ma, Yuan He, Ji-Ru Xu
AIM: To design and assess a novel protocol that employs isothermal titration calorimetry (ITC) for rapid detection of extended-spectrum β-lactamase (ESBL)-producers in clinical pathogens. METHODS AND RESULTS: A total of 69 clinical Enterobacteriaceae isolates were examined in the new ESBL-ITC test by examining the heat profiles associated with enzyme hydrolysis of different substrates (imipenem, cefotaxime and clavulanic acid). The presence of β-lactamase genes in the bacteria tested was confirmed by PCR and DNA sequencing...
September 5, 2020: Journal of Applied Microbiology
Saya Yamasaki, Katsumi Shigemura, Kayo Osawa, Koichi Kitagawa, Aya Ishii, K Kuntaman, Toshiro Shirakawa, Takayuki Miyara, Masato Fujisawa
INTRODUCTION: Extended spectrum beta-lactamase (ESBL)-producing Klebsiellapneumoniae is a serious concern for nosocomial infection and the emergence rate in Indonesia is higher than that in developed countries. The purpose of this study was to investigate the genetic characteristics of ESBL-producing K. pneumoniae isolated from UTI patients in Indonesia. MATERIALS AND METHODS: We collected K. pneumoniae resistant to ceftazidime or cefotaxime isolated from UTI patients in Dr...
September 1, 2020: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Ji Yeon Song, Sukdong Yoo, Taek Jin Lim, Joung-Hee Byun, Kyo Jin Jo, Hye-Young Kim, Su Eun Park, Seong Heon Kim
BACKGROUND: Cephalosporin is the most commonly used empirical agent for urinary tract infection in children. However, increasing use of cephalosporins can lead to an increase in resistant pathogens. Therefore, this study aims to investigate the effects of monotherapy with ampicillin-sulbactam as an alternative to cephalosporins. METHODS: All 2- to 24-month-old patients who were hospitalized at Pusan National University Children's Hospital due to a first episode of febrile UTI during the 2-year period from 2012 to 2014 were included in the study...
September 3, 2020: Pediatrics International: Official Journal of the Japan Pediatric Society
Shuaiyang Wang, Chunbo You, Fareed Qumar Memon, Geyin Zhang, Yawei Sun, Hongbin Si
The two-component system BaeSR participates in antibiotics resistance of Escherichia coli. To know whether the outer membrane proteins involve in the antibiotics resistance mediated by BaeSR, deletion of acrB was constructed and the recombined plasmid p-baeR was introduced into E. coli K12 and K12△acrB. Minimum Inhibitory concentrations (MICs) of antibacterial agents were determined by two-fold broth micro-dilution method. Gene expressions related with major outer membrane proteins and multidrug efflux pump-related genes were determined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR)...
September 3, 2020: Journal of Biochemistry
Shaymaa H Abdel-Rhman
K. pneumoniae was known as a nosocomial infection that causes human diseases. It is considered as one of the food-borne pathogens as it causes septicemia and diarrhea in humans. This study aims to characterize K. pneumoniae strains isolated from ready to eat processed meat phenotypically and genetically. Three hundred and fifty ready to eat processed meat (Luncheon-meat) samples were collected. Forty-four (12.6%) K. pneumoniae strains were isolated and bio-typed, where the majority were identified to belong to biotype B1...
2020: PloS One
S Mezghani Maalej, R Ben Abdallah, A Hammami
A retrospective study was conducted at the Sfax hospitals between 2013 and 2014 to study the susceptibility to antibiotics of Haemophilus influenzae after the introduction of Hib vaccination in Tunisia. Capsular typing was done by PCR. MICs for β-lactams were determined by E-test® . The blaTEM , blaROB and ftsI genes were searched using PCR. Among the 259 strains of H. influenzae isolated, 248 (95.7%) were non-invasive. Five strains were encapsulated (3 type b and 2 type c). Resistance rates were 33.4% for ampicillin, 10...
2020: Bulletin de la Société de Pathologie Exotique
Abhiroop Banerjee, K Batabyal, A D Singh, S N Joardar, S Dey, D P Isore, T K Sar, T K Dutta, S Bandyopadhyay, I Samanta
Antimicrobial resistance is a global emergency which needs one health approach to address. The present study was conducted to detect the prevalence of beta-lactamase and biofilm-producing-Klebsiella strains in rectal swabs (n=624) collected from healthy dogs, cats, sheep and goats reared as companion or household animals in India. The dogs and cats were frequently exposed to third or fourth generation cephalosporins for therapy.The sheep and goats were occasionally exposed to antibiotics and had environmental exposure...
September 3, 2020: Letters in Applied Microbiology
Jacob Bodilsen, Pierre Tattevin, Steven Tong, Pontus Naucler, Henrik Nielsen
To examine antimicrobial management of brain abscess and prioritize future trials. Self-administered, Internet-based survey of practices for treatment of community-acquired bacterial brain abscess among infectious diseases (ID) specialists in France, Sweden, Australia, and Denmark during November 2019. Respondents were also asked to rank future randomized controlled trials (RCTs) from 1 (high priority) to 6 (low priority). 310 ID specialists (45% female) from France (35%), Sweden (29%), Australia (25%), and Denmark (11%) participated in the survey, primarily from university hospitals (69%) with an on-site neurosurgical department (61%)...
September 2, 2020: European Journal of Clinical Microbiology & Infectious Diseases
Lorina Badger-Emeka, Yasmeen Al-Mulhim, Fatimah Al-Muyidi, Maram Busuhail, Salma Alkhalifah, Norah AlEid
Background: The 21st century has seen a wide range of diseases resulting from zoonotic infections, of which bacterial infections have led to outbreaks of food-borne diseases. Aim: The study looks at bacterial pathogen carriage by farm rats and their antimicrobial susceptibility, with the view of providing insights for antimicrobial surveillance. Method: Farm rats of Rattus rattus species where randomly collected alive from farms in Al-Ahsa using food baits...
2020: Environmental Health Insights
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