Keywords Effect of ROS on peripheral bl...

Effect of ROS on peripheral blood cells
Enrique Almanza-Aguilera, Alvaro Hernáez, Dolores Corella, Daniel Muñoz Aguayo, Emilio Ros, Olga Portolés, Julieta Valussi, Ramon Estruch, Oscar Coltell, Isaac Subirana, Jordi Salas-Salvadó, Miguel Ruiz-Canela, Rafael de la Torre, Lara Nonell, Montserrat Fitó, Olga Castañer
Background: The Traditional Mediterranean Diet (TMD) is known to have beneficial effects on several chronic diseases. However, data concerning the whole transcriptome modulation of the TMD are scarce. Objective: We aimed to explore the effects of the TMD on the whole transcriptome of individuals at high cardiovascular risk. Methods: Thirty-four participants at high cardiovascular risk were randomly assigned to a TMD enriched with extra-virgin olive oil (TMD + VOO), mixed nuts (TMD + Nuts), or a control diet based on low-fat diet recommendations...
April 15, 2020: Nutritional Neuroscience
Christien Masmeijer, Tina Rogge, Katharina van Leenen, Lieze De Cremer, Piet Deprez, Eric Cox, Bert Devriendt, Bart Pardon
In veal and dairy beef production systems, Holstein bull calves experience many stressors and excessive pathogen exposure, necessitating the use of antimicrobials for welfare and production reasons. The aim of this randomized clinical trial was to explore the effects of esterified fatty acids used as feed supplement on health, production and immune variables in veal calves. Different glycerol-esters of fatty acids were used: short chain fatty acid (SCFA)-based glycerol-mono- (C4) and tributyrate (C4), and medium chain fatty acid (MCFA)-based glycerol-monocaprylate/monocaprinate (C8/C10) and glycerol-monolaurate (C12) in two different doses...
May 2020: Preventive Veterinary Medicine
Jin He, Ru Han, Gongchang Yu, Martin F Lavin, Qiang Jia, Ping Cui, Cheng Peng
Benzene (BZ) is an important occupational and environmental pollutant. Exposure to BZ may cause aplastic anemia which is characterized as bone marrow hematopoietic failure. In order to reduce the harmful effects of this pollutant, it is necessary to identify additional preventative measures. In this study, we investigated the protective effects of epimedium polysaccharide (EPS), a natural compound with antioxidant and immune-enhancing potency, on aplastic anemia induced by benzene exposure in mice. Male CD-1 mice were randomly divided into five groups including control, BZ (880 mg/kg), LE (EPS low-dose, 20 mg/kg + BZ), ME (EPS middle-dose, 100 mg/kg + BZ), and HE (EPS high-dose, 200 mg/kg + BZ) groups...
2020: Evidence-based Complementary and Alternative Medicine: ECAM
Cristian Vergallo, Elisa Panzarini, Bernardetta Anna Tenuzzo, Stefania Mariano, Ada Maria Tata, Luciana Dini
One of the most relevant drawbacks in medicine is the ability of drugs and/or imaging agents to reach cells. Nanotechnology opened new horizons in drug delivery, and silver nanoparticles (AgNPs) represent a promising delivery vehicle for their adjustable size and shape, high-density surface ligand attachment, etc. AgNPs cellular uptake involves different endocytosis mechanisms, including lipid raft-mediated endocytosis. Since static magnetic fields (SMFs) exposure induces plasma membrane perturbation, including the rearrangement of lipid rafts, we investigated whether SMF could increase the amount of AgNPs able to pass the peripheral blood lymphocytes (PBLs) plasma membrane...
March 19, 2020: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Ampika Phutim Mangkhalthon, Aroon Teerakapong, Patcharaporn Tippayawat, Noppawan Phumala Morales, Supawich Morkmued, Subin Puasiri, Aroonsri Priprem, Teerasak Damrongrungruang
INTRODUCTION: Photodynamic therapy improves oral mucositis treatment. The reactive oxygen species (ROS) generated from this reaction could contribute to an anti-inflammatory effect by suppressing inflammatory cells. OBJECTIVE: To evaluate the anti-inflammatory effect of photodynamic therapy using guaiazulene and a red laser in peripheral blood mononuclear cells (PBMCs). METHODS: Guaiazulene solutions (1, 2, 5, 25, 35, and 100 µM in 99.8% methanol) were irradiated with red laser light (625 nm, 151...
March 18, 2020: Photodiagnosis and Photodynamic Therapy
Karin Margaretha Gilljam, Kristine Lillebø Holm, Muhammad Zahoor, Federica Grazia Centonze, Hesso Farhan, Heidi Kiil Blomhoff
It is becoming increasingly evident that reactive oxygen species (ROS) have critical roles as "second messengers" in cell signaling. In B cells, ROS can be generated either as a byproduct of mitochondrial respiration, as a result of the endoplasmic reticulum stress response induced by high production of Igs, or by the activation of NADPH oxidase (NOX) complexes. Having previously shown that costimulation of B cells via TLR 9 and the TLR-related receptor RP105 drives maturation of human peripheral blood B cells into Ig-producing cells, we aimed to study the role of ROS generated during this vital process...
March 18, 2020: Journal of Immunology
Huijing Huang, Xuesong Liu, Dandan Chen, Yikang Lu, Jia Li, Fang Du, Chunyan Zhang, Liangjing Lu
Atherosclerotic cardiovascular disease confers significant morbidity and mortality in patients with systemic lupus erythematosus (SLE). A substantial proportion of patients with SLE display accelerated endothelial dysfunction, which precedes cardiovascular disease. Melatonin and its nuclear receptor retinoid-related orphan receptor alpha (RORα) have been reported to have some protective effects on the development of atherosclerosis. However, the function of melatonin in SLE-induced endothelial dysfunction and the role that RORα plays are still unknown...
June 2020: International Immunopharmacology
Sandra Jesus, Ana Patrícia Marques, Alana Duarte, Edna Soares, João Panão Costa, Mariana Colaço, Mélanie Schmutz, Claudia Som, Gerrit Borchard, Peter Wick, Olga Borges
Nanoparticles (NPs) assumed an important role in the area of drug delivery. Despite the number of studies including NPs are growing over the last years, their side effects on the immune system are often ignored or omitted. One of the most studied polymers in the nano based drug delivery system field is chitosan (Chit). In the scientific literature, although the physicochemical properties [molecular weight (MW) or deacetylation degree (DDA)] of the chitosan, endotoxin contamination and appropriate testing controls are rarely reported, they can strongly influence immunotoxicity results...
2020: Frontiers in Bioengineering and Biotechnology
Sun Jiaxin, Wang Shengchen, Cao Yirong, Wang Shuting, Li Shu
Cadmium (Cd) is a bioaccumulative toxic heavy metal element that has been shown to cause irreversible damage to the immune system once contaminated with water, thereby jeopardizing the health of fish and other aquatic organisms. Neutrophils react against multiple invading pathogens through different mechanisms. The effect of Cd immunotoxicity in carp neutrophils has not been thoroughly studied. Here, common carp peripheral blood neutrophils were exposed to 10 μmol/L Cd for 2 h or then stimulated with 20 nmol/L PMA under laboratory conditions to study the effect and potential mechanism of Cd on neutrophils...
February 11, 2020: Fish & Shellfish Immunology
Akiyoshi Aikawa, Tomohiro Kozako, Yuichiro Uchida, Makoto Yoshimitsu, Kenji Ishitsuka, Takeo Ohsugi, Shin-Ichiro Honda
Adult T-cell leukemia/lymphoma (ATL) is an aggressive T-cell neoplasm with poor prognosis that develops after chronic infection with human T-cell leukemia virus type 1 (HTLV-1). Although AMP-activated protein kinase (AMPK) is a critical cellular energy sensor, it has recently become clear that AMPK can act as a tumor regulator. Here, we assessed the expression of AMPK in primary ATL cells and the effects of dorsomorphin, an AMPK inhibitor, on primary ATL cells and HTLV-1-infected T-cell lines. AMPK expression in acute and chronic ATL patients was significantly higher than in asymptomatic HTLV-1 carriers and healthy donors...
February 6, 2020: FEBS Journal
Gabriela Soledad Barragán-Zarate, Luicita Lagunez-Rivera, Rodolfo Solano, Elizabeth Arlen Pineda-Peña, Arizai Yolia Landa-Juárez, Aracely Evangelina Chávez-Piña, Candy Carranza-Álvarez, Diego Manuel Hernández-Benavides
ETHNOPHARMACOLOGICAL RELEVANCE: Prosthechea karwinskii (Mart.) J.M.H. Shaw is a Mexican orchid used in traditional medicine by some indigenous communities to treat issues related to inflammation (cough, wounds, burns, and diabetes). Pharmacological research of this orchid could validate its therapeutic uses and demonstrate its potential for treating other health conditions of high prevalence in Mexico, including those associated with oxidative stress such as diabetes, cancer, atherosclerosis, and hypertension as well as inflammation...
January 25, 2020: Journal of Ethnopharmacology
Andrew J Byrne, Sandra A Bright, James P McKeown, John E O'Brien, Brendan Twamley, Darren Fayne, D Clive Williams, Mary J Meegan
Lymphomas (cancers of the lymphatic system) account for 12% of malignant diseases worldwide. Burkitt's lymphoma (BL) is a rare form of non-Hodgkin's lymphoma in which the cancer starts in the immune B-cells. We report the synthesis and preliminary studies on the antiproliferative activity of a library of 9,10-dihydro-9,10-ethanoanthracene based compounds structurally related to the antidepressant drug maprotiline against BL cell lines MUTU-1 and DG-75. Structural modifications were achieved by Diels-Alder reaction of the core 9-(2-nitrovinyl)anthracene with number of dienophiles including maleic anhydride, maleimides, acrylonitrile and benzyne...
January 17, 2020: Pharmaceuticals
Krystyna Obtułowicz, Joanna Góralska, Anna Bogdali, Wojciech Dyga, Aleksander Obtułowicz, Dorota Myszkowska, Monika Ziemianin, Anna Gruca, Bogdan Solnica, Ewa Czarnobilska
INTRODUCTION: Hereditary angioedema (HAE) is a rare autosomal dominant disease caused by genetic dysfunction of C1 inhibitor (C1-INH) due to mutations in the SERPING1 gene. The disorder is mediated mainly by bradykinin. The clinical course of the disease is varied and not related to genetic changes. OBJECTIVES: We aimed to evaluate redox homeostasis of peripheral blood mononuclear cells (PBMCs) in patients with HAE due to C1-INH deficiency (C1 INH HAE) by measuring the levels of reactive oxygen species (ROS) of PBMCs as well as plasma advanced glycation end products (AGEs) and advanced oxidation protein products (AOPPs)...
February 27, 2020: Polish Archives of Internal Medicine
Wan He, Zhijian Xu, Dongliang Song, Hui Zhang, Bo Li, Lu Gao, Yong Zhang, Qilin Feng, Dandan Yu, Liangning Hu, Gege Chen, Tao Yi, Xiaosong Wu, Jumei Shi, Weiliang Zhu
AIMS: Diffuse large B-cell lymphoma (DLBCL) is one of the most aggressive lymphoid malignancies, which remains incurable, thus warranting the development of new therapies. Our previous study determined that rafoxanide is very effective in treating multiple myeloma (MM). In the present study, we tried to evaluate the effects of rafoxanide on DLBCL, as well as the potential underlying molecular mechanisms. MAIN METHODS: We used CCK-8 assay and flow cytometry to assess cell viability and apoptosis...
January 8, 2020: Life Sciences
Zonghong Li, Dadong Guo, Xuewei Yin, Shumin Ding, Mingyue Shen, Ruiming Zhang, Yan Wang, Ruirong Xu
BACKGROUND: Human multiple myeloma (MM) is a malignant and incurable B cell tumor. Zinc oxide nanoparticles (ZnO NPs) have been widely used in biomedical fields including anti-bacterial and anti-tumor. However, the influence of ZnO NPs on MM cells is still unclear. The present study aimed to investigate the effect of ZnO NPs on MM cell (a human myeloma-derived RPMI8226 cell line) death in vitro and the underlying mechanism. METHODS: The morphology of ZnO NPs was characterized by transmission electron microscopy (TEM), and the inhibitory and apoptotic effect of ZnO NPs on human MM cells was monitored by a CCK-8 method and an Annexin V-FITC/PI assay...
February 2020: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Bin Liu, Wen Cao, Jin Cheng, Sisi Fan, Shaojun Pan, Lirui Wang, Jiaqi Niu, Yunxiang Pan, Yanlei Liu, Xiyang Sun, Lijun Ma, Jie Song, Jian Ni, Daxiang Cui
Objective: To construct a novel nanoplatform GNS@CaCO3 /Ce6-NK by loading the CaCO3 -coated gold nanostars (GNSs) with Chlorin e6 molecules (Ce6) into human peripheral blood mononuclear cells (PBMCs)-derived NK cells for tumor targeted therapy. Methods: GNS@CaCO3 /Ce6 nanoparticles were prepared and characterized by TEM and UV-vis. The cell surface markers and cytokines secretion of NK cells before and after loading the GNS@CaCO3 /Ce6 nanoparticles were detected by Flow Cytometry (FCM) and ELISA...
November 2019: Cancer Biology & Medicine
Beibei Zhang, Mengnan Zeng, Meng Li, Yuxuan Kan, Benke Li, Ruiqi Xu, Yuanyuan Wu, Shengchao Wang, Xiaoke Zheng, Weisheng Feng
Corydalis humosa Migo is a traditional Chinese medicine that clears away damp heat, relieves sore. Protopine (PRO) is an alkaloid component isolated from C. humosa Migo. However, the role of protopine in acute kidney injury (AKI) has not yet been reported. This study aims to investigate the effect and mechanism of protopine isolated from C. humosa Migo on lipopolysaccharide (LPS)-induced AKI in mice. Inflammation accumulation was assessed by small animal living imaging. The blood urea nitrogen (BUN), and serum creatinine (Scr) were measured to assess the effects of protopine on renal function in LPS-induced AKI...
December 19, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
F-L Du, W-B Dong, C Zhang, Q-P Li, L Kang, X-P Lei, L Guo, X-S Zhai
OBJECTIVE: This study aimed to evaluate effect of budesonide combining Poractant Alfa on preventing bronchopulmonary dysplasia (BPD). PATIENTS AND METHODS: A total of 120 preterm infants were involved. pH value, partial pressure of oxygen (PO2), and blood gas analysis were evaluated. Peripheral blood was collected and mononuclear cells were isolated. Reactive oxygen species (ROS) in peripheral blood mononuclear cells (PBMCs) were detected with laser confocal. Sirtuin 1 (SIRT1) in PBMCs was detected using immunofluorescence...
December 2019: European Review for Medical and Pharmacological Sciences
Hiroshi Katayama
INTRODUCTION: Despite the remarkable progress achieved in the research on Alzheimer's disease (AD), its exact pathogenesis is not fully understood and effective therapies do not currently exist. In order to find effective therapy for AD, I ranged extensively over the literature and found an important paper by Tiffany and colleagues. RESULTS AND CONCLUSION: Neuroinflammation has been proposed as a possible cause or driving force of AD. The discovery by Tiffany et al...
December 17, 2019: Brain and Behavior
Chi Xin, Shi Guangliang, Zhang Qing, Liu Qingqing, Yin Hang, Zhang Yiming, Li Shu
Astilbin (ASB), a dihydroflavonol glycoside, is widely found in a variety of plants and in functional foods and acts as a powerful antioxidant. The aim of this study was to investigate the underlying mechanisms involved in the antagonistic effects of ASB on cadmium (Cd)-induced necroptosis in chicken peripheral blood lymphocytes. Peripheral blood lymphocytes were aseptically collected from Roman white hens and then randomly divided into five groups: the control group was incubated without additional reagents, while the other groups were incubated with Cd, ASB, a combination of Cd and ASB, and 0...
December 12, 2019: Ecotoxicology and Environmental Safety
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