Keywords Effect of ROS on peripheral bl...

Effect of ROS on peripheral blood cells
Xiao-Xuan Yu, Meng-Yuan Zhu, Jia-Rong Wang, Hui Li, Po Hu, Ying-Jie Qing, Xiang-Yuan Wang, Hong-Zheng Wang, Zhan-Yu Wang, Jing-Yan Xu, Qing-Long Guo, Hui Hui
Cutaneous T-cell lymphoma (CTCL) is characterized by a heterogeneous group of extranodal non-Hodgkin lymphomas, in which monoclonal T lymphocytes infiltrate the skin. LW-213, a derivative of wogonin, was found to induce cell apoptosis in chronic myeloid leukemia (CML). In this study, we investigated the effects of LW-213 on CTCL cells and the underlying mechanisms. We showed that LW-213 (1-25 μM) dose-dependently inhibited human CTCL cell lines (Hut-102, Hut-78, MyLa, and HH) with IC50 values of around 10 μM, meanwhile it potently inhibited primary leukemia cells derived from peripheral blood of T-cell lymphoma patients...
August 3, 2020: Acta Pharmacologica Sinica
Kely Campos Navegantes-Lima, Valter Vinicius Silva Monteiro, Silvia Leticia de França Gaspar, Ana Ligia de Brito Oliveira, Juliana Pinheiro de Oliveira, Jordano Ferreira Reis, Rafaelli de Souza Gomes, Caroline Azulay Rodrigues, Herta Stutz, Vanessa Sovrani, Alessandra Peres, Pedro Roosevelt Torres Romão, Marta Chagas Monteiro
Sepsis is characterized by the host's dysregulated immune response to an infection followed by a potentially fatal organ dysfunction. Although there have been some advances in the treatment of sepsis, mainly focused on broad-spectrum antibiotics, mortality rates remain high, urging for the search of new therapies. Oxidative stress is one of the main features of septic patients, so antioxidants can be a good alternative treatment. Agaricus brasiliensis is a nutraceutical rich in bioactive compounds such as polyphenols and polysaccharides, exhibiting antioxidant, antitumor, and immunomodulatory activities...
2020: Frontiers in Immunology
Jianwei Duan, Zuoguan Chen, Xiaoyu Liang, Youlu Chen, Huiyang Li, Xinxin Tian, Mingming Zhang, Xiaoli Wang, Hongfan Sun, Deling Kong, Yongjun Li, Jing Yang
Lesions of patients with peripheral artery disease (PAD) are in a harmful microenvironment, which features increased oxidative stress and inflammatory infiltration. Hence, it is essential to improve the microenvironment along with angiogenesis. In this study, metal-polyphenol capsules (Cu-EGCG), which combines the therapeutic anti-inflammatory and antioxidant activities of EGCG and the angiogenic activity of copper ions, were synthesized through coordination between EGCG and copper ions. The sustained release of the copper ions from Cu-EGCG was demonstrated in vitro, and biocompatible Cu-EGCG can scavenge intracellular ROS, reduce cell death in the presence of cytotoxic levels of ROS, and decrease the expression of pro-inflammatory cytokines (TNF-α, IL-6)...
June 15, 2020: Biomaterials
Wang Jia, Mingming Zhen, Lei Li, Chen Zhou, Zihao Sun, Shuai Liu, Zhongpu Zhao, Jie Li, Chunru Wang, Chunli Bai
Aplastic anemia (AA) is characterized as hypoplasia of bone marrow hematopoietic cells and hematopenia of peripheral blood cells. Though the supplement of exogenous erythropoietin (EPO) has been clinically approved for AA treatment, the side-effects hinder its further application. Here a robust treatment for AA induced by chemotherapy drugs is explored using gadofullerene nanoparticles (GFNPs). Methods : The gadofullerene were modified with hydrogen peroxide under alkaline conditions to become the water-soluble nanoparticles (GFNPs)...
2020: Theranostics
Ezgi Eyluel Bankoglu, Johanna Gerber, Geema Kodandaraman, Florian Seyfried, Helga Stopper
Obesity is associated with elevated cancer risk, which may be represented by elevated genomic damage. Oxidative stress plays a key role in obesity related detrimental health consequences including DNA oxidation damage. The elevated cancer risk in obesity may be a consequence. Weight loss has been shown to reduce genomic damage, but the role of oxidative stress in that has not been clarified. The aim of this study is therefore to investigate the influence of bariatric surgery induced weight loss on DNA oxidation damage in morbidly obese subjects...
May 2020: Mutation Research
D V Novikov, S G Selivanova, N V Krasnogorova, E N Gorshkova, S N Pleskova, V V Novikov, A V Karaulov
We studied changes in the transcription of genes encoding cytokines (TNF, IL-6, IL-10, and IL-32), cell activation markers (ICAM1, CD38, Fas, and FCGRIII), ROS production catalyst (NOX2), autophagy (Beclin 1, LC3B, and p62) and apoptosis (BAX, BCL2) regulators in peripheral blood mononuclear cells upon contact with quantum dots CdSe/ZnS-MPA and CdSe/CdSeZnS/ZnS-PTVP. Up-regulation of TNF, ICAM1, Fas, p62 mRNA and down-regulation of the FCGRIII and NOX2 mRNA in response to the presence of quantum dots were revealed...
June 3, 2020: Bulletin of Experimental Biology and Medicine
Franz-Xaver Reichl, Christof Högg, Fangfang Liu, Markus Schwarz, Daniel Teupser, Reinhard Hickel, Wilhelm Bloch, Helmut Schweikl, Peter Thomas, Burkhard Summer
PURPOSE: The effect of Actovegin® was investigated on PMA- and LPS-induced human peripheral blood mononuclear cells (PBMCs). METHODS: PBMCs (1 × 106 cells/ml) from five blood donors (2 f, 3 m; 45-55 years) were grown in medium and exposed to Actovegin® in the presence or absence of PMA or LPS. Supernatants were collected to assess the concentration of cytokines (TNF-α, IL-1beta, IL-6 and IL-10). The reactive oxygen species (ROS) were assessed by a ROS-GloTM H2 O2 assay...
May 23, 2020: European Journal of Applied Physiology
Jian-Yu Gu, Yu-Jie Liu, Xiang-Qing Zhu, Jia-Ying Qiu, Ying Sun
Periodontitis is a dental plaque-induced chronic inflammatory disease. Long-term exposure of the host to periodontal pathogens leads to a hyporesponsive state to the following stimulations, which is described as endotoxin tolerance. Neutrophils are the most abundant innate immune cells in the body. To clarify the roles of endotoxin tolerance in periodontitis, inflammatory responses in Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS)-tolerized neutrophils were explored in this study. Here, apoptosis and respiratory burst in neutrophils upon single or repeated P...
May 21, 2020: Inflammation
Iwona Niemietz, Abigail T Moraes, Martina Sundqvist, Kelly L Brown
Hyaluronan (HA) is a glycosaminoglycan that in its natural, high molecular mass (HMM) form, promotes tissue repair and homeostasis. With inflammation, HA metabolism and HMM HA fragmentation to low molecular mass (LMM) forms is greatly enhanced. Considerable evidence suggests that LMM HA may act as a damage-associated molecular pattern to initiate innate immune responses. However, the responsiveness of myeloid cells to LMM HA is controversial and largely unknown for neutrophils. Peripheral blood cells from healthy donors were incubated ex vivo with pharmaceutical grade HA of different molecular mass (HMM, LMM, and HA fragments <10 kDa)...
May 18, 2020: Journal of Leukocyte Biology
Christien Masmeijer, Katharina van Leenen, Lieze De Cremer, Piet Deprez, Eric Cox, Bert Devriendt, Bart Pardon
Under the present intensive rearing conditions, calves face a series of stressors and multiple pathogens often necessitating antimicrobial use. Multiple feed additives are currently explored for their ability to prevent disease and limit the use of antimicrobials. Supplementation of the polyunsaturated long chain n-3 fatty acids eicosapentaenoic (EPA) and docohexaenoic (DHA) from marine origin has been proposed as a strategy to improve immune function and prevent excessive inflammation reactions. The aim of this randomized clinical trial was to explore the effects of n-3 fatty acids (PUFAs) used as feed supplement on health, production and immune variables in a veal calf setting...
April 7, 2020: Preventive Veterinary Medicine
Tyler J Wenzel, Ellen J Gates, Athena L Ranger, Andis Klegeris
Neuroinflammation contributes to neurodegenerative disorders, including Alzheimer's disease (AD). Gut microbes are involved in regulating systemic inflammation. Short-chain fatty acids (SCFAs), which are among the many metabolites released by gut microbes, can cross the blood-brain barrier (BBB) and interact with microglia. High concentrations of individual SCFAs decrease the inflammatory responses of peripheral monocytes; therefore, we hypothesized that SCFAs act on their own or in combinations to reduce the inflammatory response of microglia...
April 22, 2020: Molecular and Cellular Neurosciences
Marlene C Gerner, Liesa S Ziegler, Ralf L J Schmidt, Martin Krenn, Fritz Zimprich, Keziban Uyanik-Ünal, Vassiliki Konstantopoulou, Sophia Derdak, Giorgia Del Favero, Ilse Schwarzinger, Kaan Boztug, Klaus G Schmetterer
The ectonucleotidase CD39 on human regulatory T-cells (Treg) is an important immune regulator which is dysregulated in autoimmune diseases and cancer immunosuppression. We here define that CD39 expression on Treg is independent of the Treg-specific transcription factors FOXP3 and HELIOS and promoted by canonical TGF-b- and mTOR-signaling. Furthermore, the TGF-b mediated upregulation of CD39 is counteracted by reactive oxygen species (ROS)-driven autophagy. In line, CD39+ peripheral blood Treg constitute a distinct lineage with low autophagic flux and absent ROS production...
April 22, 2020: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Denise Risnik, Esteban E Elías, Irene Keitelman, Ana Colado, Enrique Podaza, Gregorio Cordini, Maricef Vergara Rubio, Horacio Fernández Grecco, Raimundo Fernando Bezares, Mercedes Borge, Analía Trevani, Mirta Giordano, Carolina Jancic, Romina Gamberale
Ibrutinib is a BTK/ITK inhibitor with efficacy for the treatment of various lymphoid cancers, including CLL. Considering that innate and adaptative immune defects are a dominant feature of CLL patients, we evaluated whether in vitro ibrutinib affects the survival and function of neutrophils and γδ T cells, key players of the early immune response against microbes. Neutrophils and γδ T cells were obtained from peripheral blood of healthy donors and CLL patients. We found that ibrutinib reduces the production of reactive oxygen species (ROS) and bacteria killing capacity, and slightly impairs neutrophil extracellular traps (NETs) production without affecting bacteria-uptake and CD62L-downregulation induced by fMLP or aggregated IgG...
April 19, 2020: Leukemia & Lymphoma
Enrique Almanza-Aguilera, Alvaro Hernáez, Dolores Corella, Daniel Muñoz Aguayo, Emilio Ros, Olga Portolés, Julieta Valussi, Ramon Estruch, Oscar Coltell, Isaac Subirana, Jordi Salas-Salvadó, Miguel Ruiz-Canela, Rafael de la Torre, Lara Nonell, Montserrat Fitó, Olga Castañer
Background: The Traditional Mediterranean Diet (TMD) is known to have beneficial effects on several chronic diseases. However, data concerning the whole transcriptome modulation of the TMD are scarce. Objective: We aimed to explore the effects of the TMD on the whole transcriptome of individuals at high cardiovascular risk. Methods: Thirty-four participants at high cardiovascular risk were randomly assigned to a TMD enriched with extra-virgin olive oil (TMD + VOO), mixed nuts (TMD + Nuts), or a control diet based on low-fat diet recommendations...
April 15, 2020: Nutritional Neuroscience
Christien Masmeijer, Tina Rogge, Katharina van Leenen, Lieze De Cremer, Piet Deprez, Eric Cox, Bert Devriendt, Bart Pardon
In veal and dairy beef production systems, Holstein bull calves experience many stressors and excessive pathogen exposure, necessitating the use of antimicrobials for welfare and production reasons. The aim of this randomized clinical trial was to explore the effects of esterified fatty acids used as feed supplement on health, production and immune variables in veal calves. Different glycerol-esters of fatty acids were used: short chain fatty acid (SCFA)-based glycerol-mono- (C4) and tributyrate (C4), and medium chain fatty acid (MCFA)-based glycerol-monocaprylate/monocaprinate (C8/C10) and glycerol-monolaurate (C12) in two different doses...
May 2020: Preventive Veterinary Medicine
Jin He, Ru Han, Gongchang Yu, Martin F Lavin, Qiang Jia, Ping Cui, Cheng Peng
Benzene (BZ) is an important occupational and environmental pollutant. Exposure to BZ may cause aplastic anemia which is characterized as bone marrow hematopoietic failure. In order to reduce the harmful effects of this pollutant, it is necessary to identify additional preventative measures. In this study, we investigated the protective effects of epimedium polysaccharide (EPS), a natural compound with antioxidant and immune-enhancing potency, on aplastic anemia induced by benzene exposure in mice. Male CD-1 mice were randomly divided into five groups including control, BZ (880 mg/kg), LE (EPS low-dose, 20 mg/kg + BZ), ME (EPS middle-dose, 100 mg/kg + BZ), and HE (EPS high-dose, 200 mg/kg + BZ) groups...
2020: Evidence-based Complementary and Alternative Medicine: ECAM
Cristian Vergallo, Elisa Panzarini, Bernardetta Anna Tenuzzo, Stefania Mariano, Ada Maria Tata, Luciana Dini
One of the most relevant drawbacks in medicine is the ability of drugs and/or imaging agents to reach cells. Nanotechnology opened new horizons in drug delivery, and silver nanoparticles (AgNPs) represent a promising delivery vehicle for their adjustable size and shape, high-density surface ligand attachment, etc. AgNPs cellular uptake involves different endocytosis mechanisms, including lipid raft-mediated endocytosis. Since static magnetic fields (SMFs) exposure induces plasma membrane perturbation, including the rearrangement of lipid rafts, we investigated whether SMF could increase the amount of AgNPs able to pass the peripheral blood lymphocytes (PBLs) plasma membrane...
March 19, 2020: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Ampika Phutim Mangkhalthon, Aroon Teerakapong, Patcharaporn Tippayawat, Noppawan Phumala Morales, Supawich Morkmued, Subin Puasiri, Aroonsri Priprem, Teerasak Damrongrungruang
INTRODUCTION: Photodynamic therapy improves oral mucositis treatment. The reactive oxygen species (ROS) generated from this reaction could contribute to an anti-inflammatory effect by suppressing inflammatory cells. OBJECTIVE: To evaluate the anti-inflammatory effect of photodynamic therapy using guaiazulene and a red laser in peripheral blood mononuclear cells (PBMCs). METHODS: Guaiazulene solutions (1, 2, 5, 25, 35, and 100 µM in 99.8% methanol) were irradiated with red laser light (625 nm, 151...
March 18, 2020: Photodiagnosis and Photodynamic Therapy
Karin Margaretha Gilljam, Kristine Lillebø Holm, Muhammad Zahoor, Federica Grazia Centonze, Hesso Farhan, Heidi Kiil Blomhoff
It is becoming increasingly evident that reactive oxygen species (ROS) have critical roles as "second messengers" in cell signaling. In B cells, ROS can be generated either as a byproduct of mitochondrial respiration, as a result of the endoplasmic reticulum stress response induced by high production of Igs, or by the activation of NADPH oxidase (NOX) complexes. Having previously shown that costimulation of B cells via TLR 9 and the TLR-related receptor RP105 drives maturation of human peripheral blood B cells into Ig-producing cells, we aimed to study the role of ROS generated during this vital process...
March 18, 2020: Journal of Immunology
Huijing Huang, Xuesong Liu, Dandan Chen, Yikang Lu, Jia Li, Fang Du, Chunyan Zhang, Liangjing Lu
Atherosclerotic cardiovascular disease confers significant morbidity and mortality in patients with systemic lupus erythematosus (SLE). A substantial proportion of patients with SLE display accelerated endothelial dysfunction, which precedes cardiovascular disease. Melatonin and its nuclear receptor retinoid-related orphan receptor alpha (RORα) have been reported to have some protective effects on the development of atherosclerosis. However, the function of melatonin in SLE-induced endothelial dysfunction and the role that RORα plays are still unknown...
June 2020: International Immunopharmacology
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