Keywords Effect of ROS on peripheral bl...

Effect of ROS on peripheral blood cells
Elsa Patricia Rondon, Houda Abir Benabdoun, Francis Vallières, Maicon Segalla Petrônio, Marcio José Tiera, Mohamed Benderdour, Julio Cesar Fernandes
Purpose: Diethylaminoethyl-chitosan (DEAE-CH) is a derivative with excellent potential as a delivery vector for gene therapy applications. The aim of this study is to evaluate its toxicological profile for potential future clinical applications. Methods: An endotoxin-free chitosan (CH) modified with DEAE, folic acid (FA) and polyethylene glycol (PEG) was used to complex small interfering RNA (siRNA) and form nanoparticles (DEAE12 -CH-PEG-FA2 /siRNA). Based on the guidelines from the International Organization for Standardization (ISO), the American Society for Testing and Materials (ASTM), and the Nanotechnology Characterization Laboratory (NCL), we evaluated the effects of the interaction between these nanoparticles and blood components...
2020: International Journal of Nanomedicine
Debora Soncini, Paola Minetto, Claudia Martinuzzi, Pamela Becherini, Valeria Fenu, Fabio Guolo, Katia Todoerti, Giovanni Calice, Paola Contini, Maurizio Miglino, Giulia Rivoli, Sara Aquino, Alida Dominietto, Antonia Cagnetta, Mario Passalacqua, Santina Bruzzone, Alessio Nencioni, Massimo Zucchetti, Tommaso Ceruti, Antonino Neri, Roberto M Lemoli, Michele Cea
Metabolic reprogramming is emerging as a cancer vulnerability that could be therapeutically exploitable using different approaches, including amino acid depletion for those tumors that rely on exogenous amino acids for their maintenance. ʟ-Asparaginase (ASNase) has contributed to a significant improvement in acute lymphoblastic leukemia outcomes; however, toxicity and resistance limit its clinical use in other tumors. Here, we report that, in multiple myeloma (MM) cells, the DNA methylation status is significantly associated with reduced expression of ASNase-related gene signatures, thus suggesting ASNase sensitivity for this tumor...
September 22, 2020: Blood Advances
Anh-Tuan Tran, Jeannette Kluess, Susanne Kersten, Andreas Berk, Marleen Paulick, Dian Schatzmayr, Sven Dänicke, Jana Frahm
The main objective of this study was to evaluate the effects of sodium sulfite (SoS) treatment of maize and its impact on the porcine immune system in the presence of an LPS-induced systemic inflammation. Control maize (CON) and Fusarium-toxin contaminated maize (FUS) were wet-preserved (20% moisture) for 79 days with (+) or without (-) SoS and then included at 10% in a diet, resulting in four experimental groups: CON-, CON+, FUS-, and FUS+ with deoxynivalenol (DON) concentrations of 0.09, 0.05, 5.36, and 0...
September 9, 2020: Mycotoxin Research
Shiwangi Gupta, Radhey Shyam Sharma, Rajeev Singh
The advent of wireless technologies has revolutionized the way we communicate. The steady upsurge in the use of mobile phone all over the world in the last two decades, while triggered economic growth, has caused substantial damage to the environment, both directly and indirectly. The electromagnetic radiation generated from mobile phones, radio-based stations, and phone towers, high-voltage power lines have been reported which leads to the variety of health scares such as the risk of cancer in human beings and adverse effects in animals, birds, etc...
September 4, 2020: International Journal of Environmental Health Research
Rajender Kumar, Ruma Rani, Saroj Kumar, Khushboo Sethi, Shikha Jain, Kanisht Batra, Sanjay Kumar, B N Tripathi
Trypanosoma evansi, an extracellular haemoflagellate, has a wide range of hosts receptive and susceptible to infection, in which it revealed highly inconsistent clinical effects. Drugs used for the treatment of trypanosomosis have been utilized for more than five decades and have several problems like local and systemic toxicity. In the present investigation, imatinib and sorafenib were selected as drugs as they are reported to have the potential to cause reactive oxygen species (ROS)-mediated effect in cancer cells...
September 1, 2020: Parasitology Research
Alexander J Currie, Ellen T Main, Heather M Wilson, Darius Armstrong-James, Adilia Warris
Excessive inflammation by phagocytes during Aspergillus fumigatus infection is thought to promote lung function decline in CF patients. CFTR modulators have been shown to reduce A. fumigatus colonization in vivo , however, their antifungal and anti-inflammatory mechanisms are unclear. Other treatments including azithromycin and acebilustat may dampen Aspergillus -induced inflammation due to their immunomodulatory properties. Therefore, we set out in this study to determine the effects of current CF therapies on ROS production and fungal killing, either direct or indirect by enhancing antifungal immune mechanisms in peripheral blood immune cells from CF patients upon A...
2020: Frontiers in Cellular and Infection Microbiology
Sânzio Silva Santana, Thassila Nogueira Pitanga, Jeanne Machado de Santana, Dalila Lucíola Zanette, Jamile de Jesus Vieira, Sètondji Cocou Modeste Alexandre Yahouédéhou, Corynne Stéphanie Ahouefa Adanho, Sayonara de Melo Viana, Nivea Farias Luz, Valeria Matos Borges, Marilda Souza Goncalves
The excessive release of heme during hemolysis contributes to the severity of sickle cell anemia (SCA) by exacerbating hemoglobin S (HbS) autoxidation, inflammation and systemic tissue damage. The present study investigated the effect of hydroxyurea (HU) on free radical neutralization and its stimulation of antioxidant genes in human peripheral blood mononuclear cells (PBMC) and human umbilical vein endothelial cells (HUVEC) in the presence or absence of hemin. HU (100 and 200 μM) significantly reduced the production of intracellular reactive oxygen species (ROS) induced by hemin at 70 μM in HUVEC...
2020: Frontiers in Immunology
Xiao-Xuan Yu, Meng-Yuan Zhu, Jia-Rong Wang, Hui Li, Po Hu, Ying-Jie Qing, Xiang-Yuan Wang, Hong-Zheng Wang, Zhan-Yu Wang, Jing-Yan Xu, Qing-Long Guo, Hui Hui
Cutaneous T-cell lymphoma (CTCL) is characterized by a heterogeneous group of extranodal non-Hodgkin lymphomas, in which monoclonal T lymphocytes infiltrate the skin. LW-213, a derivative of wogonin, was found to induce cell apoptosis in chronic myeloid leukemia (CML). In this study, we investigated the effects of LW-213 on CTCL cells and the underlying mechanisms. We showed that LW-213 (1-25 μM) dose-dependently inhibited human CTCL cell lines (Hut-102, Hut-78, MyLa, and HH) with IC50 values of around 10 μM, meanwhile it potently inhibited primary leukemia cells derived from peripheral blood of T-cell lymphoma patients...
August 3, 2020: Acta Pharmacologica Sinica
Kely Campos Navegantes-Lima, Valter Vinicius Silva Monteiro, Silvia Leticia de França Gaspar, Ana Ligia de Brito Oliveira, Juliana Pinheiro de Oliveira, Jordano Ferreira Reis, Rafaelli de Souza Gomes, Caroline Azulay Rodrigues, Herta Stutz, Vanessa Sovrani, Alessandra Peres, Pedro Roosevelt Torres Romão, Marta Chagas Monteiro
Sepsis is characterized by the host's dysregulated immune response to an infection followed by a potentially fatal organ dysfunction. Although there have been some advances in the treatment of sepsis, mainly focused on broad-spectrum antibiotics, mortality rates remain high, urging for the search of new therapies. Oxidative stress is one of the main features of septic patients, so antioxidants can be a good alternative treatment. Agaricus brasiliensis is a nutraceutical rich in bioactive compounds such as polyphenols and polysaccharides, exhibiting antioxidant, antitumor, and immunomodulatory activities...
2020: Frontiers in Immunology
Jianwei Duan, Zuoguan Chen, Xiaoyu Liang, Youlu Chen, Huiyang Li, Xinxin Tian, Mingming Zhang, Xiaoli Wang, Hongfan Sun, Deling Kong, Yongjun Li, Jing Yang
Lesions of patients with peripheral artery disease (PAD) are in a harmful microenvironment, which features increased oxidative stress and inflammatory infiltration. Hence, it is essential to improve the microenvironment along with angiogenesis. In this study, metal-polyphenol capsules (Cu-EGCG), which combines the therapeutic anti-inflammatory and antioxidant activities of EGCG and the angiogenic activity of copper ions, were synthesized through coordination between EGCG and copper ions. The sustained release of the copper ions from Cu-EGCG was demonstrated in vitro, and biocompatible Cu-EGCG can scavenge intracellular ROS, reduce cell death in the presence of cytotoxic levels of ROS, and decrease the expression of pro-inflammatory cytokines (TNF-α, IL-6)...
June 15, 2020: Biomaterials
Wang Jia, Mingming Zhen, Lei Li, Chen Zhou, Zihao Sun, Shuai Liu, Zhongpu Zhao, Jie Li, Chunru Wang, Chunli Bai
Aplastic anemia (AA) is characterized as hypoplasia of bone marrow hematopoietic cells and hematopenia of peripheral blood cells. Though the supplement of exogenous erythropoietin (EPO) has been clinically approved for AA treatment, the side-effects hinder its further application. Here a robust treatment for AA induced by chemotherapy drugs is explored using gadofullerene nanoparticles (GFNPs). Methods : The gadofullerene were modified with hydrogen peroxide under alkaline conditions to become the water-soluble nanoparticles (GFNPs)...
2020: Theranostics
Ezgi Eyluel Bankoglu, Johanna Gerber, Geema Kodandaraman, Florian Seyfried, Helga Stopper
Obesity is associated with elevated cancer risk, which may be represented by elevated genomic damage. Oxidative stress plays a key role in obesity related detrimental health consequences including DNA oxidation damage. The elevated cancer risk in obesity may be a consequence. Weight loss has been shown to reduce genomic damage, but the role of oxidative stress in that has not been clarified. The aim of this study is therefore to investigate the influence of bariatric surgery induced weight loss on DNA oxidation damage in morbidly obese subjects...
May 2020: Mutation Research
D V Novikov, S G Selivanova, N V Krasnogorova, E N Gorshkova, S N Pleskova, V V Novikov, A V Karaulov
We studied changes in the transcription of genes encoding cytokines (TNF, IL-6, IL-10, and IL-32), cell activation markers (ICAM1, CD38, Fas, and FCGRIII), ROS production catalyst (NOX2), autophagy (Beclin 1, LC3B, and p62) and apoptosis (BAX, BCL2) regulators in peripheral blood mononuclear cells upon contact with quantum dots CdSe/ZnS-MPA and CdSe/CdSeZnS/ZnS-PTVP. Up-regulation of TNF, ICAM1, Fas, p62 mRNA and down-regulation of the FCGRIII and NOX2 mRNA in response to the presence of quantum dots were revealed...
June 3, 2020: Bulletin of Experimental Biology and Medicine
Franz-Xaver Reichl, Christof Högg, Fangfang Liu, Markus Schwarz, Daniel Teupser, Reinhard Hickel, Wilhelm Bloch, Helmut Schweikl, Peter Thomas, Burkhard Summer
PURPOSE: The effect of Actovegin® was investigated on PMA- and LPS-induced human peripheral blood mononuclear cells (PBMCs). METHODS: PBMCs (1 × 106 cells/ml) from five blood donors (2 f, 3 m; 45-55 years) were grown in medium and exposed to Actovegin® in the presence or absence of PMA or LPS. Supernatants were collected to assess the concentration of cytokines (TNF-α, IL-1beta, IL-6 and IL-10). The reactive oxygen species (ROS) were assessed by a ROS-GloTM H2 O2 assay...
May 23, 2020: European Journal of Applied Physiology
Jian-Yu Gu, Yu-Jie Liu, Xiang-Qing Zhu, Jia-Ying Qiu, Ying Sun
Periodontitis is a dental plaque-induced chronic inflammatory disease. Long-term exposure of the host to periodontal pathogens leads to a hyporesponsive state to the following stimulations, which is described as endotoxin tolerance. Neutrophils are the most abundant innate immune cells in the body. To clarify the roles of endotoxin tolerance in periodontitis, inflammatory responses in Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS)-tolerized neutrophils were explored in this study. Here, apoptosis and respiratory burst in neutrophils upon single or repeated P...
May 21, 2020: Inflammation
Iwona Niemietz, Abigail T Moraes, Martina Sundqvist, Kelly L Brown
Hyaluronan (HA) is a glycosaminoglycan that in its natural, high molecular mass (HMM) form, promotes tissue repair and homeostasis. With inflammation, HA metabolism and HMM HA fragmentation to low molecular mass (LMM) forms is greatly enhanced. Considerable evidence suggests that LMM HA may act as a damage-associated molecular pattern to initiate innate immune responses. However, the responsiveness of myeloid cells to LMM HA is controversial and largely unknown for neutrophils. Peripheral blood cells from healthy donors were incubated ex vivo with pharmaceutical grade HA of different molecular mass (HMM, LMM, and HA fragments <10 kDa)...
May 18, 2020: Journal of Leukocyte Biology
Christien Masmeijer, Katharina van Leenen, Lieze De Cremer, Piet Deprez, Eric Cox, Bert Devriendt, Bart Pardon
Under the present intensive rearing conditions, calves face a series of stressors and multiple pathogens often necessitating antimicrobial use. Multiple feed additives are currently explored for their ability to prevent disease and limit the use of antimicrobials. Supplementation of the polyunsaturated long chain n-3 fatty acids eicosapentaenoic (EPA) and docohexaenoic (DHA) from marine origin has been proposed as a strategy to improve immune function and prevent excessive inflammation reactions. The aim of this randomized clinical trial was to explore the effects of n-3 fatty acids (PUFAs) used as feed supplement on health, production and immune variables in a veal calf setting...
April 7, 2020: Preventive Veterinary Medicine
Tyler J Wenzel, Ellen J Gates, Athena L Ranger, Andis Klegeris
Neuroinflammation contributes to neurodegenerative disorders, including Alzheimer's disease (AD). Gut microbes are involved in regulating systemic inflammation. Short-chain fatty acids (SCFAs), which are among the many metabolites released by gut microbes, can cross the blood-brain barrier (BBB) and interact with microglia. High concentrations of individual SCFAs decrease the inflammatory responses of peripheral monocytes; therefore, we hypothesized that SCFAs act on their own or in combinations to reduce the inflammatory response of microglia...
April 22, 2020: Molecular and Cellular Neurosciences
Marlene C Gerner, Liesa S Ziegler, Ralf L J Schmidt, Martin Krenn, Fritz Zimprich, Keziban Uyanik-Ünal, Vassiliki Konstantopoulou, Sophia Derdak, Giorgia Del Favero, Ilse Schwarzinger, Kaan Boztug, Klaus G Schmetterer
The ectonucleotidase CD39 on human regulatory T-cells (Treg) is an important immune regulator which is dysregulated in autoimmune diseases and cancer immunosuppression. We here define that CD39 expression on Treg is independent of the Treg-specific transcription factors FOXP3 and HELIOS and promoted by canonical TGF-b- and mTOR-signaling. Furthermore, the TGF-b mediated upregulation of CD39 is counteracted by reactive oxygen species (ROS)-driven autophagy. In line, CD39+ peripheral blood Treg constitute a distinct lineage with low autophagic flux and absent ROS production...
April 22, 2020: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Denise Risnik, Esteban E Elías, Irene Keitelman, Ana Colado, Enrique Podaza, Gregorio Cordini, Maricef Vergara Rubio, Horacio Fernández Grecco, Raimundo Fernando Bezares, Mercedes Borge, Analía Trevani, Mirta Giordano, Carolina Jancic, Romina Gamberale
Ibrutinib is a BTK/ITK inhibitor with efficacy for the treatment of various lymphoid cancers, including CLL. Considering that innate and adaptative immune defects are a dominant feature of CLL patients, we evaluated whether in vitro ibrutinib affects the survival and function of neutrophils and γδ T cells, key players of the early immune response against microbes. Neutrophils and γδ T cells were obtained from peripheral blood of healthy donors and CLL patients. We found that ibrutinib reduces the production of reactive oxygen species (ROS) and bacteria killing capacity, and slightly impairs neutrophil extracellular traps (NETs) production without affecting bacteria-uptake and CD62L-downregulation induced by fMLP or aggregated IgG...
April 19, 2020: Leukemia & Lymphoma
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