Keywords Effect of ROS on peripheral bl...

Effect of ROS on peripheral blood cells
Martina Sandonà, Lorena Di Pietro, Federica Esposito, Alessia Ventura, Antonietta Rosa Silini, Ornella Parolini, Valentina Saccone
Mesenchymal stromal cells (MSCs) are multipotent cells found in different tissues: bone marrow, peripheral blood, adipose tissues, skeletal muscle, perinatal tissues, and dental pulp. MSCs are able to self-renew and to differentiate into multiple lineages, and they have been extensively used for cell therapy mostly owing to their anti-fibrotic and immunoregulatory properties that have been suggested to be at the basis for their regenerative capability. MSCs exert their effects by releasing a variety of biologically active molecules such as growth factors, chemokines, and cytokines, either as soluble proteins or enclosed in extracellular vesicles (EVs)...
2021: Frontiers in Bioengineering and Biotechnology
Anna K Lundberg, Rosanna W S Chung, Louise Zeijlon, Gustav Fernström, Lena Jonasson
BACKGROUND: Inflammation and oxidative stress form a vicious circle in atherosclerosis. Oxidative stress can have detrimental effects on T cells. A unique subset of CD4+ T cells, known as regulatory T (Treg ) cells, has been associated with atheroprotective effects. Reduced numbers of Treg cells is a consistent finding in patients with chronic coronary syndrome (CCS). However, it is unclear to what extent these cells are sensitive to oxidative stress. In this pilot study, we tested the hypothesis that oxidative stress might be a potential contributor to the Treg cell deficit in CCS patients...
June 3, 2021: Journal of Translational Medicine
Hassan Mohammadlou, Maryam Hamzeloo-Moghadam, Amir Yami, Fatemeh Feizi, Marzieh Moeinifard, Ahmad Gharehbaghian
Since chemotherapy drugs have dose-related side effects, there is still a need for finding new agents with suitable cytotoxic effects without any harmful effects. For this purpose, we evaluated the cytotoxic effects of Britannin that is a Sesquiterpene Lactone compound Inula aucheriana , alone or in combination with Vincristine (VCR), on Acute Lymphoblastic Leukemia (ALL)-derived MOLT-4 cells. In this study, we found that Britannin decreased the viability of MOLT-4 cells with the IC50 Values of 2 µM, but had no cytotoxic effects on normal cells or Peripheral Blood Mononuclear Cells (PBMCs)...
June 1, 2021: Nutrition and Cancer
Mohsin Ali Khan, Sahabjada Siddiqui, Imran Ahmad, Romila Singh, Durga Prasad Mishra, Anand Narain Srivastava, Rumana Ahmad
Ajwa dates (Phoenix dactylifera L.) have been described in traditional and alternative medicine to provide several health benefits, but their mechanism of apoptosis induction against human triple-negative breast cancer MDA-MB-231 cells remains to be investigated. In this study, we analyzed the phytoconstituents in ethanolic Ajwa Dates Pulp Extract (ADPE) by liquid chromatography-mass spectrometry (LC-MS) and investigated anticancer effects against MDA-MB-231 cells. LC-MS analysis revealed that ADPE contained phytocomponents belonging to classes such as carbohydrates, phenolics, flavonoids and terpenoids...
May 14, 2021: Scientific Reports
Annemieke M Peters van Ton, Harmke B Duindam, Julia van Tuijl, Wilson Wl Li, Hendrik-Jan Dieker, Niels P Riksen, Fj Anton Meijer, Roy Pc Kessels, Nils Kohn, Johannes G van der Hoeven, Peter Pickkers, Mark Rijpkema, Wilson F Abdo
INTRODUCTION: Postoperative cognitive dysfunction occurs frequently after coronary artery bypass grafting (CABG). The underlying mechanisms remain poorly understood, but neuroinflammation might play a pivotal role. We hypothesise that systemic inflammation induced by the surgical trauma could activate the innate immune (glial) cells of the brain. This could lead to an exaggerated neuroinflammatory cascade, resulting in neuronal dysfunction and loss of neuronal cells. Therefore, the aims of this study are to assess neuroinflammation in vivo presurgery and postsurgery in patients undergoing major cardiac surgery and investigate whether there is a relationship of neuroinflammation to cognitive outcomes, changes to brain structure and function, and systemic inflammation...
May 11, 2021: BMJ Open
VinayKumar Tripathi, Deepa Bhartiya, Ashok Vaid, Sagar Chhabria, Nripen Sharma, Bipin Chand, Vaishnavi Takle, Pratiksha Palahe, Ashish Tripathi
Cancer is a devastating disease whose incidence has increased in recent times and early detection can lead to effective treatment. Existing detection tools suffer from low sensitivity and specificity, and are high cost, invasive and painful procedures. Cancers affecting different tissues, ubiquitously express embryonic markers including Oct-4A, whose expression levels have also been correlated to staging different types of cancer. Cancer stem cells (CSCs) that initiate cancer are possibly the 'transformed' and pluripotent very small embryonic-like stem cells (VSELs) that also express OCT-4A...
May 6, 2021: Stem cell reviews and reports
Kinga Kik, Bożena Bukowska, Anita Krokosz, Paulina Sicińska
With the ongoing commercialization, human exposure to plastic nanoparticles will dramatically increase, and evaluation of their potential toxicity is essential. There is an ongoing discussion on the human health effects induced by plastic particles. For this reason, in our work, we assessed the effect of polystyrene nanoparticles (PS-NPs) of various diameters (29, 44 and 72 nm) on selected parameters of oxidative stress and the viability of human peripheral blood mononuclear cells (PBMCs) in the in vitro system...
April 23, 2021: International Journal of Molecular Sciences
Olga Witkowska-Piłaszewicz, Rafał Pingwara, Jarosław Szczepaniak, Anna Winnicka
Clenbuterol, the β2-adrenoceptor agonist, is gaining growing popularity because of its effects on weight loss (i.e., chemical liposuction). It is also popular in bodybuilding and professional sports, due to its effects that are similar to anabolic steroids. However, it is prohibited by anti-doping control. On the other hand, it is suggested that clenbuterol can inhibit the inflammatory process. The cells from 14 untrained and 14 well-trained race horses were collected after acute exercise and cultured with clenbuterol...
April 17, 2021: Cells
Jasmine Karacs, Manuel Reithofer, Claudia Kitzmüller, Markus Kraller, Stefanie Schmalz, Sonja Bleichert, Johannes B Huppa, Hannes Stockinger, Barbara Bohle, Beatrice Jahn-Schmid
Aluminum hydroxide (alum) and monophosphoryl-lipid A (MPLA) are conventional adjuvants in vaccines for allergen-specific immunotherapy (AIT). Alum triggers the release of neutrophil extracellular traps (NETs) by neutrophils. NETs contain expelled decondensed chromatin associated with granular material and may act as danger-associated molecular patterns and activate antigen-presenting cells. We investigated whether adjuvant-induced NETs contribute to innate responses to AIT-vaccines. Human neutrophils were incubated with alum, MPLA and adjuvant-containing AIT-vaccine preparations...
April 1, 2021: Vaccines
Brooke J Wanrooy, Shu Wen Wen, Connie Hy Wong
Clinical trials involving the blockage of peripheral inflammatory leukocyte recruitment into the brain has puzzlingly led to either no significant improvement in stroke outcome, or even worsened outcomes and increased mortality, prompting a re-evaluation of our understanding into the neuroinflammatory processes after stroke. Whilst traditionally understood as simple effectors of the innate immune system, emerging research in vascular disease biology has redefined the neutrophil as a specialised and highly specific cell type with dynamic functional capacity...
April 24, 2021: Immunology and Cell Biology
B Zou, R-X Zhuang, X-Y Sun, J Liang
OBJECTIVE: Bone marrow mesenchymal stem cells (BMSCs) have immunomodulatory and therapeutic effects on immune system diseases. This study intends to assess the regulatory effect of BMSC targeted therapy on the IL-17+ γδ T cells and Treg cells in allergic rhinitis (AR). MATERIALS AND METHODS: BALB/c mice were sensitized by ovalbumin (OVA), while BMSCs were injected intravenously before sensitization and followed by an analysis of nasal symptoms, inflammation, cytokines, and immunoglobulins...
April 2021: European Review for Medical and Pharmacological Sciences
Mojdeh Barati, Mohammad Amin Javidi, Behrad Darvishi, Seyed Peyman Shariatpanahi, Zahra S Mesbah Moosavi, Reyhane Ghadirian, Tahereh Khani, Hassan Sanati, Hossein Simaee, Mahdieh Shokrollahi Barough, Leila Farahmand, Alireza Madjid Ansari
Whereas the anti-neoplastic activity of extremely low frequency magnetic fields (ELF-EMF) is well-documented in literature, little is known about its underlying anti-cancer mechanisms and induced types of cell death. Here, for the first time, we reported induction of necroptosis, a specific type of programed necrotic cell death, in MC4-L2 breast cancer cell lines following a 2 h/day exposure to a 100 Hz, 1 mT ELF-EMF for five days. For in vivo assessment, inbred BALB/c mice bearing established MC-4L2 tumors were exposed to 100 mT, 1 Hz ELF-EMF 2 h daily for a period of 28-day, following which tumors were dissected and fixed for evaluation of tumor biomarkers expression and types of cell death induced using TUNEL assay, Immunohistochemistry and H&E staining...
April 18, 2021: Free Radical Biology & Medicine
Mar Sempere-Bigorra, Iván Julián-Rochina, Omar Cauli
BACKGROUND: Diabetic neuropathy is defined as the dysfunction of the peripheral nervous system in diabetic patients. It is considered a microvascular complication of diabetes mellitus. Its presence is associated with increased morbidity and mortality. Although several studies have found alterations at somatic motor, sensory levels and at the level of autonomic nervous system in diabetic patients, there is not a systematic approach regarding the differences in neuropathy between the major variants of diabetes, e...
March 22, 2021: Journal of Personalized Medicine
Andrana K Calgarotto, Ana L Longhini, Fernando V Pericole de Souza, Adriana S Santos Duarte, Karla P Ferro, Irene Santos, Victor Maso, Sara T Olalla Saad, Cristiane Okuda Torello
Green tea (GT) treatment was evaluated for its effect on the immune and antineoplastic response of elderly acute myeloid leukemia patients with myelodysplasia-related changes (AML-MRC) who are ineligible for aggressive chemotherapy and bone marrow transplants. The eligible patients enrolled in the study (n = 10) received oral doses of GT extract (1000 mg/day) alone or combined with low-dose cytarabine chemotherapy for at least 6 months and/or until progression. Bone marrow (BM) and peripheral blood (PB) were evaluated monthly...
January 2021: Integrative Cancer Therapies
Shirin Saberianpour, Mohamad Hadi Saeed Modaghegh, Hamidreza Rahimi, Mohammad Mahdi Kamyar
Varicose veins are the most common vascular disease in humans. Veins have valves that help the blood return gradually to the heart without leaking blood. When these valves become weak, blood and fluid collect and pool by pressing against the walls of the veins, causing varicose veins. In the cardiovascular system, mechanical forces are important determinants of vascular homeostasis and pathological processes. Blood vessels are constantly exposed to a variety of hemodynamic forces, including shear stress and environmental strains caused by the blood flow...
February 2021: Biophysical Reviews
Xuhui Ge, Pengyu Tang, Yuluo Rong, Dongdong Jiang, Xiao Lu, Chengyue Ji, Jiaxing Wang, Chenyu Huang, Ao Duan, Yang Liu, Xinglin Chen, Xichen Chen, Zhiyang Xu, Feng Wang, Zibin Wang, Xiaoyan Li, Wene Zhao, Jin Fan, Wei Liu, Guoyong Yin, Weihua Cai
Pathologically, blood-spinal-cord-barrier (BSCB) disruption after spinal cord injury (SCI) leads to infiltration of numerous peripheral macrophages into injured areas and accumulation around newborn vessels. Among the leaked macrophages, M1-polarized macrophages are dominant and play a crucial role throughout the whole SCI process. The aim of our study was to investigate the effects of M1-polarized bone marrow-derived macrophages (M1-BMDMs) on vascular endothelial cells and their underlying mechanism. Microvascular endothelial cell line bEnd...
March 5, 2021: Redox Biology
Jose A Adams, Arkady Uryash, Jose R Lopez, Marvin A Sackner
Diabetes has reached worldwide epidemic proportions, and threatens to be a significant economic burden to both patients and healthcare systems, and an important driver of cardiovascular mortality and morbidity. Improvement in lifestyle interventions (which includes increase in physical activity via exercise) can reduce diabetes and cardiovascular disease mortality and morbidity. Encouraging a population to increase physical activity and exercise is not a simple feat particularly in individuals with co-morbidities (obesity, heart disease, stroke, peripheral vascular disease, and those with cognitive and physical limitations)...
2021: Frontiers in Physiology
Jun Dou, Luoyang Li, Mei Guo, Feng Mei, Danfeng Zheng, Hui Xu, Rui Xue, Xueyang Bao, Fengshu Zhao, Yu Zhang
Background and Aim: Acute myeloid leukemia (AML), initiated and maintained by leukemia stem cells (LSCs), is often relapsed or refractory to therapy. The present study aimed at assessing the effects of nanozyme-like Fe3 O4 nanoparticles (IONPs) combined with cytosine arabinoside (Ara-C) on LSCs in vitro and in vivo. Methods: The CD34+ CD38- LSCs, isolated from human AML cell line KG1a by a magnetic activated cell sorting method, were treated with Ara-C, IONPs, and Ara-C+ IONPs respectively in vitro...
2021: International Journal of Nanomedicine
Ali Reza Bastin, Mahdieh Nazari-Robati, Hossein Sadeghi, Amir Hossein Doustimotlagh, Asie Sadeghi
Background : Evidence has shown that inflammation and oxidative stress are implicated in the development of a great number of human diseases. Trehalose possesses various biological effects including antioxidant and anti-inflammatory activities. However, there is little data on the effects of trehalose on human cells including peripheral blood mononuclear cells (PBMCs). Here, we aimed to investigate whether trehalose could attenuate oxidative stress and inflammation induced by lipopolysaccharides (LPS) in PBMCs...
February 25, 2021: Immunological Investigations
ChengYu Mao, DongJiu Li, En Zhou, JunFeng Zhang, ChangQian Wang, Chao Xue
Evidence suggests that nicotine intake promotes atherosclerosis. We enrolled 100 patients with coronary heart disease (CHD) and found that plaque burden, TXNIP expression, and inflammatory chemokine levels were higher in smokers than non-smokers. Additionally, patients with higher TXNIP expression in peripheral blood mononuclear cells (PBMCs) had a higher Gensini Scores and higher plasma IL-1β and IL-18 levels. Treating bone marrow-derived macrophages (BMDMs) with nicotine in vitro led to enhanced lipid phagocytosis, chemotaxis, and increased production of reactive oxygen species (ROS), which activated TXNIP/NLRP3 inflammasome signaling and promoted pyroptosis, as evidenced by caspase-1 cleavage and increased production of IL-1β, IL-18, and gasdermin D...
February 24, 2021: Aging
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