Yingying Liu, Rong Guo, Shuwu Zhang, Yuhuan Sun, Fayuan Wang
Microplastics (MPs) are emerging contaminants in terrestrial systems that cause diverse impacts on plants. However, little is known about whether MPs especially micro-sized MPs can be accumulated and translocated in plants particularly food crops. Hereby, a hydroponic experiment was conducted to verify whether nano-sized (80 nm) and micro-sized (1 µm) fluorescently labeled polystyrene (PS) microspheres can enter rice roots and translocate to aerial parts. Plant samples were taken for detection of PS after 14 days and 40 days exposure, respectively...
July 21, 2021: Journal of Hazardous Materials
Dandan Song, Haoyu Lyu, Qiushi Feng, Jiangti Luo, Lin Li, Xiaosheng Wang
Although head and neck squamous cell cancer (HNSCC) is one of the cancer types in which immune checkpoint inhibitors (ICIs) has achieved a certain success, only a subset of HNSCC patients respond to ICIs. Thus, identification of HNSCC subtypes responsive to ICIs is crucial. Using hierarchical clustering, we identified three subtypes of HNSCC, termed Immunity-H, Immunity-M, and Immunity-L, based on the enrichment scores of 28 immune cells generated by the single-sample gene-set enrichment analysis of transcriptome data...
July 28, 2021: International Immunopharmacology
Wei Wang, Rui Bai, Xiantao Song
In order to determine the function of dHAND in a specific subset of cardiomyocyte progenitor cells responsible for the heart and its function in the cellular mechanism of Hand2-/- mouse ventricular hypoplasia, we established an allelic knockout model of dHAND in human embryonic stem cells (hESCs-H9) by an episomal vector-based CRISPR/Cas9 system. This dHAND KO hESC line maintained normal karyotype and typical primed pluripotent human stem cell morphology, and maintained pluripotency, could differentiate into all three germ layers in vivo...
July 20, 2021: Stem Cell Research
N S Remya, S Syama, V Gayathri, H K Varma, P V Mohanan
No abstract text is available yet for this article.
July 28, 2021: Colloids and Surfaces. B, Biointerfaces
Sercan Keskin, Carly Pawell, Niels de Jonge
Graphene liquid cells (GLCs) present the thinnest possible sample enclosures for liquid phase electron microscopy. However, the actual presence of liquid within a GLC is not always guaranteed. Of key importance is to reliably test the presence of the liquid, which is most frequently water or saline. Here, the commonly used methods for verifying the presence of water were evaluated. It is shown that depending on the type of sample, applying a single criterion does not always conclusively verify the presence of water...
June 30, 2021: Micron
Chufan Liu, Rongbo Xiao, Fei Huang, Xiaojun Yang, Weijie Dai, Meili Xu
Lead (Pb) is widely distributed in the environment that can impose potential risks to vegetables and humans. In this work, we conducted a pot experiment in Southern China to examine the physiological response and risk of edible amaranth (Amaranthus tricolor L.) under the simultaneous stresses of lead from soil and atmosphere. The results indicate that the lead content of amaranth substantially exceeded China's national standard when Pb concentration from soils and atmosphere was high, and comparing to teenagers and adults, children exposed a higher health risk after consuming the contaminated amaranth...
July 28, 2021: Ecotoxicology and Environmental Safety
Xinxin Xie, Jinxiao Chen, Da Wo, En Ma, Yongling Ning, Jun Peng, Weidong Zhu, Dan-Ni Ren
ETHNOPHARMACOLOGICAL RELEVANCE: Traditional Chinese Medicine (TCM) is being increasingly used worldwide due to its diverse efficacy and relatively low side effects. Babao Dan (BBD) is a well-known TCM formula that is currently used for the effective treatment of various cancers, however its underlying molecular mechanism remains unknown. AIM OF THE STUDY: Tumor growth and tumor recurrence are characterized by two distinct populations of cells, namely the well-differentiated cancer cells composing the majority of tumor bulk, and cancer stem cells (CSCs) involved in tumor relapse, which are both strongly associated with excessive activation of Wnt/β-catenin signaling...
July 28, 2021: Journal of Ethnopharmacology
Ning Zhang, Xiaohui Hua, Huailu Tu, Jingxia Li, Zhuo Zhang, Costa Max
Epithelial mesenchymal transition (EMT) is highly correlated with metastasis during cancer development. Although previous studies have revealed that ISO is able to inhibit cancer cell invasion and stem-cell properties, little is known about the effects of ISO on EMT markers. The present study explores the potential regulation of ISO on EMT, leading to the inhibition of migration and invasion of bladder cancer cells. We found that ISO inhibited Vimentin, one of the EMT markers, in the invasive bladder cancer cell lines U5637 and T24T...
July 28, 2021: Cancer Letters
Wisberty J Gordián-Vélez, Dimple Chouhan, Rodrigo A España, H Isaac Chen, Jason A Burdick, John E Duda, D Kacy Cullen
Parkinson's disease is a neurodegenerative disease affecting around 10 million people worldwide. The death of dopaminergic neurons in the substantia nigra and the axonal fibers that constitute the nigrostriatal pathway leads to a loss of dopamine in the striatum that causes the motor symptoms of this disease. Traditional treatments have focused on reducing symptoms, while therapies with human fetal or stem cell-derived neurons have centered on implanting these cells in the striatum to restore its innervation...
July 28, 2021: Brain Research Bulletin
Stephan A Ramos, John J Morton, Prabha Yadav, Brendan Reed, Sheila I Alizadeh, Ali H Shilleh, Loni Perrenoud, James Jaggers, John Kappler, Antonio Jimeno, Holger A Russ
BACKGROUND: The thymus is a glandular organ that is essential for the formation of the adaptive immune system by educating developing T cells. The thymus is most active during childhood and involutes around the time of adolescence resulting in a severe reduction or absence of naïve T cell output. The ability to generate a patient derived human thymus would provide an attractive research platform and enable the development of novel cell therapies. OBJECTIVE: We sought to systematically evaluate signaling pathways to develop a refined direct differentiation protocol that generates patient derived thymic epithelial progenitor cells (pTEPs) from multiple iPSCs, that can further differentiate into functional thymic epithelial cells (pTECs) upon transplantation into athymic nude mice...
July 28, 2021: Journal of Allergy and Clinical Immunology
Asra Almubarak, Rotem Lavy, Nikola Srnic, Yawen Hu, Devi Priyanka Maripuri, Tsutomo Kume, Fred B Berry
Endochondral ossification initiates the growth of the majority of the mammalian skeleton and is tightly controlled through gene regulatory networks. The forkhead box transcription factors Foxc1 and Foxc2 have been demonstrated to regulate aspects of osteoblast function in the formation of the skeleton but their roles in chondrocytes to control endochondral ossification are less clear. Here we demonstrate that Foxc1 expression is directly regulated by the activity of SOX9, one of the earliest transcription factors to specify the chondrocyte lineage...
July 28, 2021: Journal of Biological Chemistry
Mahdieh Alipour, Sadaf Fadakar, Marziyeh Aghazadeh, Roya Salehi, Hossein Samadi Kafil, Leila Roshangar, Ensieh Mousavi, Zahra Aghazadeh
Curcumin (CUR) is an ancient therapeutic agent with remarkable antimicrobial and anti-inflammatory properties. The purpose of the current study was to synthesize and evaluate a curcumin-based reparative endodontic material to reduce infection and inflammation besides the induction of mineralization during the healing of the dentin-pulp complex. Poly-ɛ-caprolactone (PCL)/gelatin (Gel)/CUR scaffold was synthesized and assessed by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermo-gravimetric analysis (TGA)...
July 31, 2021: Journal of Biochemical and Molecular Toxicology
Margit Rosner, Markus Hengstschläger
3D cell migration is an integral part of many physiologic processes. Although being well studied in the context of adult tissue homeostasis and cancer development, remarkably little is known about the invasive behavior of human stem cells. Using two different kinds of invasion assays, this study aimed at investigating and characterizing the 3D migratory capacity of human amniotic fluid stem cells (hAFSCs), a well-established fetal stem cell type. Eight hAFSC lines were found to harbour pronounced potential to penetrate basement membrane (BM)-like matrices...
July 31, 2021: Stem Cells
Zhi-Feng Jiang, Yiru Shao, Lin Zhang, Jie Shen
Phosgene may induce acute lung injury (ALI) when a person is exposed to it. Mesenchymal stem cells (MSCs) were affirmed to have therapeutic effects on phosgene-induced ALI. In a previous study, ALI exosomes have been confirmed to promote the proliferation and migration of MSCs. However, the mechanism of this phenomenon is still unclear. MicroRNAs (miRNAs) are essential in the physiological process of cells. In this study, lung-derived exosomes were isolated from phosgene-exposed and normal rats, respectively, through ultracentrifugation and cultured MSCs with these exosomes...
July 31, 2021: Journal of Biochemical and Molecular Toxicology
Tatsuya Yuikawa, Masaaki Ikeda, Sachiko Tsuda, Shinji Saito, Kyo Yamasu
In vertebrate embryogenesis, elongation of the posterior body is driven by de novo production of the axial and paraxial mesoderm as well as the neural tube at the posterior end. This process is presumed to depend on the stem cell-like population in the tail bud region, but the details of the gene regulatory network involved are unknown. Previous studies suggested the involvement of pou5f3, an Oct4-type POU gene in zebrafish, in axial elongation. In the present study, we first found that pou5f3 is expressed mainly in the dorsal region of the tail bud immediately after gastrulation, and that this expression is restricted to the posterior-most region of the elongating neural tube during somitogenesis...
July 31, 2021: Development, Growth & Differentiation
Nalee Kim, Hoon Do Lim, Sang Eun Yoon, Seok Jin Kim, Won Seog Kim
INTRODUCTION: We aimed to investigate the role of upfront whole-brain radiation therapy (RT), with a reduced dose of 23.4 Gy, following high-dose methotrexate (HD-MTX) in patients with primary central nervous system lymphoma (PCNSL). METHODS: We retrospectively reviewed 185 patients with PCNSL treated with HD-MTX between January 2013 and January 2020; 145 patients underwent no RT and 40 patients underwent upfront RT. Using propensity score matching (PSM) to adjust for clinical factors, 40 patients were selected from each treatment group...
July 31, 2021: Journal of Neuro-oncology
Zhen Sun, Hua Yu, Jing Zhao, Tianyu Tan, Hongru Pan, Yuqing Zhu, Lang Chen, Cheng Zhang, Li Zhang, Anhua Lei, Yuyan Xu, Xianju Bi, Xin Huang, Bo Gao, Longfei Wang, Cristina Correia, Ming Chen, Qiming Sun, Yu Feng, Li Shen, Hao Wu, Jianlong Wang, Xiaohua Shen, George Q Daley, Hu Li, Jin Zhang
LIN28 is an RNA binding protein with important roles in early embryo development, stem cell differentiation/reprogramming, tumorigenesis and metabolism. Previous studies have focused mainly on its role in the cytosol where it interacts with Let-7 microRNA precursors or mRNAs, and few have addressed LIN28's role within the nucleus. Here, we show that LIN28 displays dynamic temporal and spatial expression during murine embryo development. Maternal LIN28 expression drops upon exit from the 2-cell stage, and zygotic LIN28 protein is induced at the forming nucleolus during 4-cell to blastocyst stage development, to become dominantly expressed in the cytosol after implantation...
July 31, 2021: Protein & Cell
Benjamin Sundqvist, Harri Sihto, Maria von Willebrand, Tom Böhling, Virve Koljonen
Merkel cell carcinoma (MCC) is a rare and aggressive neuroendocrine malignancy of the skin. The cell of origin of MCC is thus far unknown and proposed cells of origin include Merkel cells, pro-/pre- or pre-B cells, epithelial stem cells, and dermal stem cells. In this study, we aimed to shed further light on the possibility that a subset of MCC tumors arise from epithelial stem cells of the skin by examining the expression of hair follicle and epidermal stem cell markers in MCC and normal human skin. We also aimed to elucidate any correlation between the expression of these markers and tumor Merkel cell polyomavirus (MCPyV) status or other clinicopathological characteristics or patient survival...
July 31, 2021: Virchows Archiv: An International Journal of Pathology
(no author information available yet)
No abstract text is available yet for this article.
September 1, 2021: Endocrinology
Alessandra Soriente, Ines Fasolino, Alejandro Gomez-Sánchez, Evgen Prokhorov, Giovanna Giuliana Buonocore, Gabriel Luna-Barcenas, Luigi Ambrosio, Maria Grazia Raucci
Considerable attention has been given to the use of chitosan (CS)-based materials reinforced with inorganic bioactive signals such as hydroxyapatite (HA) to treat bone defects and tissue loss. It is well known that CS/HA based materials possess minimal foreign body reactions, good biocompatibility, controlled biodegradability and antibacterial property. Herein, the bioactivity of these composite systems was analyzed on in vitro bone cell models for their applications in the field of bone tissue engineering (BTE)...
July 31, 2021: Journal of Biomedical Materials Research. Part A
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