keyword
https://read.qxmd.com/read/38543623/a-novel-transposon-tn-7709-harbors-multidrug-resistance-genes-in-a-pathogenic-aeromonas-media-strain-qst31
#1
JOURNAL ARTICLE
Baodi Shang, Xiaoyi Li, Xiaoping Zhang, Meiyan Zhang, Jie Kong, Jinle Wang, Aiping Tan, Feng Zhao, Defeng Zhang
Pathogenic Aeromonas spp. are the etiological agents of Motile Aeromonas Septicemia (MAS). This study aimed to identify the pathogen of diseased tadpoles ( Quasipaa spinosa ) and the antibiotic-resistance characteristics of this bacterium. A Gram-negative bacterium, named strain QST31, was isolated from the ascites of diseased tadpoles and was identified as Aeromonas media based on physiological and biochemical tests, as well as molecular identification. Artificial infection experiments showed that strain QST31 was highly virulent to tadpoles, with an LC50 of 2...
March 13, 2024: Microorganisms
https://read.qxmd.com/read/38534119/functional-versatility-of-zur-in-metal-homeostasis-motility-biofilm-formation-and-stress-resistance-in-yersinia-pseudotuberculosis
#2
JOURNAL ARTICLE
Yanchao Gu, Yongde Liu, Wei Mao, Ying Peng, Xiaoru Han, Han Jin, Jingling Xu, Liyang Chang, Yixin Hou, Xihui Shen, Xingyu Liu, Yantao Yang
UNLABELLED: Zur (zinc uptake regulator) is a significant member of the Fur (ferric uptake regulator) superfamily, which is widely distributed in bacteria. Zur plays crucial roles in zinc homeostasis and influences cell development and environmental adaptation in various species. Yersinia pseudotuberculosis is a Gram-negative enteric that pathogen usually serves as a model organism in pathogenicity studies. The regulatory effects of Zur on the zinc transporter ZnuABC and the protein secretion system T6SS have been documented in Y...
March 27, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38531781/role-of-type-vi-secretion-system-in-pathogenic-remodeling-of-host-gut-microbiota-during-aeromonas-veronii-infection
#3
JOURNAL ARTICLE
Xiaoli Jiang, Hanzeng Li, Jiayue Ma, Hong Li, Xiang Ma, Yanqiong Tang, Juanjuan Li, Xue Chi, Yong Deng, Sheng Zeng, Zhu Liu
Intestinal microbial disturbance is a direct cause of host disease. The bacterial Type VI Secretion System (T6SS) often plays a crucial role in the fitness of pathogenic bacteria by delivering toxic effectors into target cells. However, its impact on the gut microbiota and host pathogenesis is poorly understood. To address this question, we characterized a new T6SS in the pathogenic Aeromonas veronii C4. First, we validated the secretion function of the core machinery of A. veronii C4 T6SS. Second, we found that the pathogenesis and colonization of A...
March 26, 2024: ISME Journal
https://read.qxmd.com/read/38527976/identification-of-non-canonical-antagonistic-bacteria-via-interspecies-contact-dependent-killing
#4
JOURNAL ARTICLE
Long Lin, Min Tao, Wei-Mei He, Qian-Hua Wu, Hao-Kai Huang, Aprodisia Kavutu Murero, Xiao-Long Shao, Li-Min Wang, Guo-Liang Qian
BACKGROUND: Canonical biocontrol bacteria were considered to inhibit pathogenic bacteria mainly by secreting antibiotic metabolites or enzymes. Recent studies revealed that some biocontrol bacteria can inhibit pathogenic bacteria through contact-dependent killing (CDK) mediated by contact-dependent secretion systems. The CDK was independent of antibiotic metabolites and often ignored in normal biocontrol activity assay. RESULTS: In this study, we aimed to use a pathogen enrichment strategy to isolate non-canonical bacteria with CDK ability...
March 25, 2024: Pest Management Science
https://read.qxmd.com/read/38520915/the-expanding-universe-of-contractile-injection-systems-in-bacteria
#5
REVIEW
Lin Lin
Contractile injection systems (CISs) are phage tail-like machineries found in a wide range of bacteria. They are often deployed by bacteria to translocate effectors into the extracellular space or into target cells. CISs are classified into intracellular type VI secretion systems (T6SSs) and extracellular CIS (eCISs). eCISs are assembled in cytoplasm and released into the extracellular milieu upon cell lysis, while T6SSs are the secretion systems widespread among Gram-negative bacteria and actively translocate effectors into the environment or into the adjacent cell, without lysis of T6SS-producing cells...
March 22, 2024: Current Opinion in Microbiology
https://read.qxmd.com/read/38499217/construction-and-analysis-of-the-immune-effect-of-two-different-vaccine-types-based-on-vibrio-harveyi-vgrg
#6
JOURNAL ARTICLE
Xiangyu Du, Minjie Kang, Chunhuan Yang, Xinping Yao, Lvliang Zheng, Ying Wu, Panpan Zhang, Han Zhang, Yongcan Zhou, Yun Sun
Vibrio harveyi poses a significant threat to fish and invertebrates in mariculture, resulting in substantial financial repercussions for the aquaculture sector. Valine-glycine repeat protein G (VgrG) is essential for the type VI secretion system's (T6SS) assembly and secretion. VgrG from V. harveyi QT520 was cloned and analyzed in this study. The localization of VgrG was determined by Western blot, which revealed that it was located in the cytoplasm, secreted extracellularly, and attached to the membrane. The effectiveness of two vaccinations against V...
March 16, 2024: Fish & Shellfish Immunology
https://read.qxmd.com/read/38497656/type-1-fimbriae-mediated-collective-protection-against-type-6-secretion-system-attacks
#7
JOURNAL ARTICLE
Margot Marie Dessartine, Artemis Kosta, Thierry Doan, Éric Cascales, Jean-Philippe Côté
Bacterial competition may rely on secretion systems such as the type 6 secretion system (T6SS), which punctures and releases toxic molecules into neighboring cells. To subsist, bacterial targets must counteract the threats posed by T6SS-positive competitors. In this study, we used a comprehensive genome-wide high-throughput screening approach to investigate the dynamics of interbacterial competition. Our primary goal was to identify deletion mutants within the well-characterized E. coli K-12 single-gene deletion library, the Keio collection, that demonstrated resistance to T6SS-mediated killing by the enteropathogenic bacterium Cronobacter malonaticus ...
March 18, 2024: MBio
https://read.qxmd.com/read/38476100/a-type-vi-secretion-system-effector-tseg-of-pantoea-ananatis-is-involved-in-virulence-and-antibacterial-activity
#8
JOURNAL ARTICLE
Xiaozhen Zhao, Lu Gao, Qurban Ali, Chenjie Yu, Bingqin Yuan, Hai Huang, Juying Long, Qin Gu, Huijun Wu, Xuewen Gao
The type VI secretion system (T6SS) of many gram-negative bacteria injects toxic effectors into adjacent cells to manipulate host cells during pathogenesis or to kill competing bacteria. However, the identification and function of the T6SS effectors remains only partly known. Pantoea ananatis, a gram-negative bacterium, is commonly found in various plants and natural environments, including water and soil. In the current study, genomic analysis of P. ananatis DZ-12 causing brown stalk rot on maize demonstrated that it carries three T6SS gene clusters, namely, T6SS-1, T6SS-2, and T6SS-3...
March 2024: Molecular Plant Pathology
https://read.qxmd.com/read/38468848/secretory-molecules-from-secretion-systems-fine-tune-the-host-beneficial-bacteria-pgprs-interaction
#9
REVIEW
Garima Gupta, Puneet Singh Chauhan, Prabhat Nath Jha, Rakesh Kumar Verma, Sachidanand Singh, Virendra Kumar Yadav, Dipak Kumar Sahoo, Ashish Patel
Numerous bacterial species associate with plants through commensal, mutualistic, or parasitic association, affecting host physiology and health. The mechanism for such association is intricate and involves the secretion of multiple biochemical substances through dedicated protein systems called secretion systems SS. Eleven SS pathways deliver protein factors and enzymes in their immediate environment or host cells, as well as in competing microbial cells in a contact-dependent or independent fashion. These SS are instrumental in competition, initiation of infection, colonization, and establishment of association (positive or negative) with host organisms...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38452551/evfg-is-a-multi-function-protein-located-in-the-type-vi-secretion-system-for-expec
#10
JOURNAL ARTICLE
Wenjia Lu, Hao Lu, Xinyu Huo, Chenchen Wang, Zhaoran Zhang, Bingbing Zong, Gaoyan Wang, Wenqi Dong, Xiaodan Li, Yuying Li, Huanchun Chen, Chen Tan
The Type VI secretion system (T6SS) functions as a protein transport nanoweapon in several stages of bacterial life. Even though bacterial competition is the primary function of T6SS, different bacteria exhibit significant variations. Particularly in Extraintestinal pathogenic Escherichia coli (ExPEC), research into T6SS remains relatively limited. This study identified the uncharacterized gene evfG within the T6SS cluster of ExPEC RS218. Through our experiments, we showed that evfG is involved in T6SS expression in ExPEC RS218...
February 23, 2024: Microbiological Research
https://read.qxmd.com/read/38436228/a-combination-of-genomics-and-transcriptomics-provides-insights-into-the-distribution-and-differential-mrna-expression-of-type-vi-secretion-system-in-clinical-klebsiella-pneumoniae
#11
JOURNAL ARTICLE
Wanzhen Li, Xiaolan Huang, Dan Li, Xiaofen Liu, Xiaoying Jiang, Xingchen Bian, Xin Li, Jing Zhang
UNLABELLED: The type VI secretion system (T6SS) serves as a crucial molecular weapon in interbacterial competition and significantly influences the adaptability of bacteria in their ecological niche. However, the distribution and function of T6SS in clinical Klebsiella pneumoniae , a common opportunistic nosocomial pathogen, have not been fully elucidated. Here, we conducted a genomic analysis of 65 clinical K. pneumoniae isolates obtained from patients with varying infections. Genes encoding a T6SS cluster present in all analyzed strains of K...
March 4, 2024: MSphere
https://read.qxmd.com/read/38435022/constitutive-expression-of-the-type-vi-secretion-system-carries-no-measurable-fitness-cost-in-vibrio-cholerae
#12
JOURNAL ARTICLE
Christopher Zhang, Sayantan Datta, William C Ratcliff, Brian K Hammer
The Type VI Secretion System (T6SS) is a widespread and highly effective mechanism of microbial warfare; it confers the ability to efficiently kill susceptible cells within close proximity. Due to its large physical size, complexity, and ballistic basis for intoxication, it has widely been assumed to incur significant growth costs in the absence of improved competitive outcomes. In this study, we precisely examine the fitness costs of constitutive T6SS firing in the bacterium Vibrio cholerae . We find that, contrary to expectations, constitutive expression of the T6SS has a negligible impact on growth, reducing growth fitness by 0...
March 2024: Ecology and Evolution
https://read.qxmd.com/read/38426053/agrobacteria-deploy-two-classes-of-his-me-finger-superfamily-nuclease-effectors-exerting-different-antibacterial-capacities-against-specific-bacterial-competitors
#13
JOURNAL ARTICLE
Mary Nia M Santos, Katherine L Pintor, Pei-Yu Hsieh, Yee-Wai Cheung, Li-Kang Sung, Yu-Ling Shih, Erh-Min Lai
The type VI secretion system (T6SS) assembles into a contractile nanomachine to inject effectors across bacterial membranes for secretion. The Agrobacterium tumefaciens species complex is a group of soil inhabitants and phytopathogens that deploys T6SS as an antibacterial weapon against bacterial competitors at both inter-species and intra-species levels. The A. tumefaciens strain 1D1609 genome encodes one main T6SS gene cluster and four vrgG genes (i.e., vgrGa-d ), each encoding a spike protein as an effector carrier...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38384301/the-role-of-type-vi-secretion-system-genes-in-antibiotic-resistance-and-virulence-in-acinetobacter-baumannii-clinical-isolates
#14
JOURNAL ARTICLE
Pu Li, Sirui Zhang, Jingdan Wang, Mona Mohamed Al-Shamiri, Kai Luo, Shuyan Liu, Peng Mi, Xiaokang Wu, Haiping Liu, Huohuan Tian, Bei Han, Jin'e Lei, Shaoshan Han, Lei Han
INTRODUCTION: The type VI secretion system (T6SS) is a crucial virulence factor in the nosocomial pathogen Acinetobacter baumannii . However, its association with drug resistance is less well known. Notably, the roles that different T6SS components play in the process of antimicrobial resistance, as well as in virulence, have not been systematically revealed. METHODS: The importance of three representative T6SS core genes involved in the drug resistance and virulence of A...
2024: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/38376647/the-role-of-the-type-vi-secretion-system-in-the-stress-resistance-of-plant-associated-bacteria
#15
REVIEW
Rui Yin, Juanli Cheng, Jinshui Lin
The type VI secretion system (T6SS) is a powerful bacterial molecular weapon that can inject effector proteins into prokaryotic or eukaryotic cells, thereby participating in the competition between bacteria and improving bacterial environmental adaptability. Although most current studies of the T6SS have focused on animal bacteria, this system is also significant for the adaptation of plant-associated bacteria. This paper briefly introduces the structure and biological functions of the T6SS. We summarize the role of plant-associated bacterial T6SS in adaptability to host plants and the external environment, including resistance to biotic stresses such as host defenses and competition from other bacteria...
February 20, 2024: Stress Biol
https://read.qxmd.com/read/38376387/-shigella-sonnei-utilises-colicins-during-inter-bacterial-competition
#16
JOURNAL ARTICLE
P B Leung, X M Matanza, B Roche, K P Ha, H C Cheung, S Appleyard, T Collins, O Flanagan, B S Marteyn, A Clements
The mammalian colon is one of the most densely populated habitats currently recognised, with 1011 -1013 commensal bacteria per gram of colonic contents. Enteric pathogens must compete with the resident intestinal microbiota to cause infection. Among these enteric pathogens are Shigella species which cause approximately 125 million infections annually, of which over 90 % are caused by Shigella flexneri and Shigella sonnei. Shigella sonnei was previously reported to use a Type VI Secretion System (T6SS) to outcompete E...
February 2024: Microbiology
https://read.qxmd.com/read/38373891/comparative-genomics-and-host-range-analysis-of-four-ralstonia-pseudosolanacearum-strains-isolated-from-sunflower-reveals-genomic-and-phenotypic-differences
#17
JOURNAL ARTICLE
Shanwen Ding, Zijun Ma, Lin Yu, Guobing Lan, Yafei Tang, Zhenggang Li, Zifu He, Xiaoman She
BACKGROUND: Bacterial wilt caused by Ralstonia solanacearum species complex (RSSC) is one of the devastating diseases in crop production, seriously reducing the yield of crops. R. pseudosolanacearum, is known for its broad infrasubspecific diversity and comprises 36 sequevars that are currently known. Previous studies found that R. pseudosolanacearum contained four sequevars (13, 14, 17 and 54) isolated from sunflowers sown in the same field. RESULTS: Here, we provided the complete genomes and the results of genome comparison of the four sequevars strains (RS639, RS642, RS647, and RS650)...
February 19, 2024: BMC Genomics
https://read.qxmd.com/read/38373044/comparative-phenotype-and-transcriptome-analysis-revealed-the-role-of-ferric-uptake-regulator-fur-in-the-virulence-of-vibrio-harveyi-isolated-from-diseased-american-eel-anguilla-rostrata
#18
JOURNAL ARTICLE
Qijuan Wan, Shaowei Zhai, Minxia Chen, Ming Xu, Songlin Guo
Vibrio harveyi is commonly found in salt and brackish water and is recognized as a serious bacterial pathogen in aquaculture worldwide. In this study, we cloned the ferric uptake regulator (fur) gene from V. harveyi wild-type strain HA_1, which was isolated from diseased American eels (Anguilla rostrata) and has a length of 450 bp, encoding 149 amino acids. Then, a mutant strain, HA_1-Δfur, was constructed through homologous recombination of a suicide plasmid (pCVD442). The HA_1-Δfur mutant exhibited weaker biofilm formation and swarming motility, and 18-fold decrease (5...
February 19, 2024: Journal of Fish Diseases
https://read.qxmd.com/read/38367471/avian-pathogenic-escherichia-coli-t6ss-effector-protein-hcp2a-causes-mitochondrial-dysfunction-through-interaction-with-letm1-protein-in-df-1-cells
#19
JOURNAL ARTICLE
Liting Lu, Zhao Qi, Zhe Chen, Haiyang Wang, Xiyang Wei, Bingyu Zhao, Zhenyu Wang, Ying Shao, Jian Tu, Xiangjun Song
The type VI secretion system (T6SS) of avian pathogenic Escherichia coli (APEC) can affect the functions of eukaryotic cells by secreting or injecting effectors. Hemolysin co-regulatory protein (Hcp), one of the markers of the T6SS, is both a structural protein and an effector protein of the T6SS. According to previous studies, mitochondria in eukaryotic cells are targeted by pathogenic bacteria. However, little is known about the regulation of mitochondria in eukaryotic host cells by the T6SS effector protein Hcp of APEC...
February 1, 2024: Poultry Science
https://read.qxmd.com/read/38365258/bacterial-type-vi-secretion-system-helps-prevent-cheating-in-microbial-communities
#20
JOURNAL ARTICLE
Alain Filloux
No abstract text is available yet for this article.
January 8, 2024: ISME Journal
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