Stem cell transplant harvest

Shuxian Zhao, Yan Liu, Zenghui Pu
Background: Acute liver failure is an inflammation-mediated hepatocyte injury. Mesenchymal stem cell (MSC) transplantation is currently considered to be an effective treatment strategy for acute liver failure. Exosomes are an important paracrine factor that can be used as a direct therapeutic agent. However, the use of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) in the treatment of acute liver failure has not been reported. Purpose: Here, we established a model of hepatocyte injury and apoptosis induced by D-galactosamine and lipopolysaccharide (D-GalN/LPS) to study the protective effect of BMSC-Exos on hepatocyte apoptosis, and further explored its protective mechanism...
2019: Drug Design, Development and Therapy
Akitoshi Inui, Hidekazu Sekine, Kazunori Sano, Izumi Dobashi, Azumi Yoshida, Katsuhisa Matsuura, Eiji Kobayashi, Minoru Ono, Tatsuya Shimizu
Introduction: The definitive treatment for severe heart failure is transplantation. However, only a small number of heart transplants are performed each year due to donor shortages. Therefore, novel treatment approaches based on artificial organs or regenerative therapy are being developed as alternatives. We have developed a technology known as cell sheet-based tissue engineering that enables the fabrication of functional three-dimensional (3D) tissue. Here, we report a new technique for engineering human cardiac tissue with perfusable blood vessels...
December 2019: Regenerative Therapy
Bianca Vezzani, Mario Gomez-Salazar, Joan Casamitjana, Carlo Tremolada, Bruno Péault
In the past decade, adipose tissue transplants have been widely used in plastic surgery and orthopaedics to enhance tissue repletion and/or regeneration. Accordingly, techniques for harvesting and processing human adipose tissue have evolved in order to quickly and efficiently obtain large amounts of tissue. Among these, the closed system technology represents an innovative and easy-to-use system to harvest, process, and re-inject refined fat tissue in a short time and in the same intervention (intra-operatively)...
October 20, 2019: Journal of Visualized Experiments: JoVE
Lihua Dong, Murat Gul, Simone Hildorf, Susanne Elisabeth Pors, Stine Gry Kristensen, Eva R Hoffmann, Dina Cortes, Jorgen Thorup, Claus Yding Andersen
Spermatogonial stem cell (SSC) transplantation therapy is a promising strategy to renew spermatogenesis for prepubertal boys whose fertility is compromised. However, propagation of SSCs is required due to a limited number of SSCs in cryopreserved testicular tissue. This propagation must be done under xeno-free conditions for clinical application. SSCs were propagated from infant testicular tissue (7 mg and 10 mg) from two boys under xeno-free conditions using human platelet lysate and nutrient source. We verified SSC-like cell clusters (SSCLCs) by quantitative real-time polymerase chain reaction (PCR) and immune-reaction assay using the SSC markers undifferentiated embryonic cell transcription factor 1 (UTF1), ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), GDNF receptor alpha-1 (GFRα-1) Fα and promyelocytic leukaemia zinc finger protein (PLZF)...
October 29, 2019: International Journal of Molecular Sciences
Marcella Reale, Isabella Gonzales-Portillo, Cesar V Borlongan
Alzheimer's disease (AD) is a progressive and multifactorial disease. Many scientific advances have advanced our understanding of the pathogenesis of AD. However, the clinical diagnosis of AD remains difficult, with only post-mortem assays confirming its definitive diagnosis. There is a crucial need for an early and accurate detection of AD related symptoms. To date, current diagnosis techniques are costly or invasive. Finding a peripheral biomarker that could provide a sensitive, reproducible, and accurate detection prior to the onset of the AD clinical symptoms will allow identification of "at risk" individuals, thereby facilitating early initiation of treatments that may prove more effective...
October 25, 2019: Brain Research
S Grobbelaar, A E Mercier, E Verburgh, D Brittain, M S Pepper
The growing need for haematopoietic stem cell transplantation (HSCT) is reflected in the increasing number of transplants performed globally each year. HSCT provides life-changing and potentially curative therapy for a range of pathologies including haematological malignancies; other indications include certain congenital and acquired disorders of the haematopoietic system, autoimmune conditions and hereditary diseases. The primary goals of HSCT are either to replace haematopoietic stem and progenitor cells (HSPC) following myeloablative chemotherapy or to cure the original pathology with allogeneic HSPCs...
September 10, 2019: South African Medical Journal
Takashi Saito, Takako Sato, Koichi Suzuki
Many cell types maintain their function short-term after death. Stem cells isolated from postmortem tissues have been successfully applied in transplantation studies. However, stem cell viability and stemness are reported to decline with increased time after death. Although postmortem stem cells may be useful for regenerative therapy and forensic diagnostics, their characteristic remain to be better understood. Adipose-derived mesenchymal stromal/stem cells (ASCs) have the capacity to differentiate through several cell lineages and are able to survive in an ischemic environment for a prolonged time...
October 16, 2019: Journal of Forensic and Legal Medicine
Eliska Furlong, Jesper Jensen, Mark Woodard, Katherine Griffiths, Geoff Knight, Marian Sturm, Fiona Kerr, Hazel Gough, Natasha Bear, Tina L Carter, Catherine H Cole, Rishi S Kotecha, Shanti Ramachandran
BACKGROUND: Autologous hematopoietic stem cell transplantation (aHSCT) using hematopoietic progenitor cells (HPCs) has become an important therapeutic modality for patients with high-risk malignancies. Current literature on standardized method for HPC apheresis in children is sparse and failure rate reported as high as 30%. PATIENTS/METHODS: A retrospective study of 125 pediatric patients with high-risk malignancies undergoing aHSCT in Western Australia between 1997 and 2016 was conducted...
October 20, 2019: Pediatric Transplantation
Üstün Yilmaz, Ozan Salim, Orhan K Yücel, Utku Iltar, Levent Ündar
BACKGROUND: The aim of this study was to evaluate the efficacy of cyclophosphamide-based (CB) and platinum-based (PB) chemotherapy regimens for hematopoietic stem cell mobilization in patients with Multiple Myeloma (MM), Hodgkin's lymphoma (HL), and non-Hodgkin's lymphoma (NHL) and the less well-known IEV (iphosphamide, epirubicin, etoposide) regimen in terms of stem cell harvesting competence, factors affecting stem cell com-petence, and toxicity. METHODS: A retrospective evaluation was made of 203 patients (94 MM, 37 HL, and 72 NHL) with peripheral blood stem cell mobilization in different chemotherapy regimens between 2000 and 2010 at the Department of He-matology, Faculty of Medicine, Akdeniz University...
October 1, 2019: Clinical Laboratory
Takashi I, Yoshinori Sumita, Takako Yoshida, Ryo Honma, Mayumi Iwatake, Jorge Luis Montenegro Raudales, Tomoko Shizuno, Shinichiro Kuroshima, Haruchika Masuda, Makoto Seki, Simon D Tran, Takayuki Asahara, Izumi Asahina
BACKGROUND: There are currently no effective treatments available for patients with irreversible loss of salivary gland (SG) function caused by radiation therapy for head and neck cancer. In this study, we have developed an effective culture method to enhance the anti-inflammatory and vasculogenic phenotypes of peripheral blood mononuclear cells (PBMNCs) and investigated whether such effectively conditioned PBMNCs (E-MNCs) could regenerate radiation-injured SGs and ameliorate salivary secretory function in mice...
October 17, 2019: Stem Cell Research & Therapy
Olga Zając-Spychała, Anna Pieczonka, Małgorzata Barańska, Jacek Wachowiak
BACKGROUND: Despite the fact that the choice of donors and the number of sources of hematopoietic stem cells have increased, a sibling remains a preferred donor for allogeneic hematopoietic stem cell transplantation (HSCT). Transplant donation between siblings is a unique life experience that may have an impact on their future relationship. The aim of the study was to quantitatively measure the quality of life (QoL) in transplanted patients and to describe the relationship between a recipient and a sibling donor after HSCT...
October 14, 2019: Biology of Blood and Marrow Transplantation
Mai Nanya, Kimiko Yurugi, Itaru Kato, Hidefumi Hiramatsu, Hiroshi Kawabata, Tadakazu Kondo, Tomoki Iemura, Rie Hishida, Erika Shibutani, Keiko Matsui, Yoko Nakagawa, Norimi Niwa, Yasunari Kasai, Joseph M Roig, Yasuyuki Arai, Yasuo Miura, Akifumi Takaori-Kondo, Taira Maekawa, Hideyo Hirai
Granulocyte transfusion (GTX) is a therapeutic option for severe bacterial or fungal infection in patients with sustained neutropenia after chemotherapy or stem cell transplantation. However, high molecular weight hydroxyethyl starch (HES), which has been used for selective sedimentation of red blood cells during apheresis, is not easily available in many countries including Japan. In this study, we evaluated the efficiency of granulocyte collection using medium molecular weight HES (130 kDa) in combination with the Spectra Optia apheresis system...
October 10, 2019: International Journal of Hematology
J F Gaviño, M García, P Dominguez, J Caviedes, B Martin, F Abella, M C Manzanares
AIM: This case report demonstrates a positive outcome of the adjuvant use of fragile fracture (FF), which is a technique used to harvest Dental pulp stem cells (DPSCs), and Platelet Rich Plasma (PRP) in a mandibular premolar (tooth 44) with a completely formed root that was transplanted into a surgically created socket and which maintained pulp vitality and function. SUMMARY: After virtual Surgical Planning, a 3D Tooth Replica of tooth 44 was fabricated. A surgical socket was created in the position of tooth 14 and then tooth 44 was extracted and the root dentine was abraded using a turbine diamond bur at 3 mm from the apex until a circular indentation was prepared around the outer circumference of the root and then an FF was performed without damaging the pulp tissue...
October 6, 2019: International Endodontic Journal
Daphne Hingert, Helena Barreto Henriksson, Adad Baranto, Helena Brisby
Low back pain is one of the most common disorders and believed to be due to intervertebral discs (IVDs) degeneration. Transplantation of human mesenchymal stem cells (hMSCs) is suggested as potential treatment option. BMP-3 growth factor promotes chondrogenesis and is proven effective in enhancing chondrogenesis in hMSCs pretreated with IL-1beta in hydrogel model. 3D co-cultures of hMSCs and disc cells (DCs) have previously been demonstrated to result in increased proteoglycan production. The aim was to study the effects of BMP-3 on hMSCs, DCs as well as hMSCs and DCs in co-culture in a pellet system, both as single treatment and after pre-treatment (PRE-T) of IL-1β...
October 3, 2019: Tissue Engineering. Part A
Mohammad Kreimei, Nir Sorkin, Adi Einan-Lifshitz, David S Rootman, Clara C Chan
OBJECTIVE: To investigate the ocular surface stability of donor eyes after conjunctival limbal autograft (CLAU) or conjunctival limbal allograft (CLAL). DESIGN: Retrospective interventional case series. PARTICIPANTS: Patients diagnosed with limbal stem cell deficiency (LSCD) who underwent ocular surface stem cell transplantation with postoperative follow-up of at least 3 months. METHODS: Donor eye data collected included preoperative and postoperative Snellen best-corrected visual acuity (BCVA), intraoperative and postoperative complications, re-epithelialization times, and ocular surface stability at the last follow-up...
October 2019: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
Frank Kroschinsky, Denise Röllig, Barbara Riemer, Michael Kramer, Rainer Ordemann, Johannes Schetelig, Martin Bornhäuser, Gerhard Ehninger, Mathias Hänel
BACKGROUND: The combination of dexamethasone, high-dose cytarabine, and cisplatin (DHAP) is an established salvage regimen for lymphoma patients. We hypothesized that a modified administration schedule for cisplatin and cytarabine results in lower toxicity and improved efficacy. METHODS: We retrospectively analysed 119 patients with relapsed or refractory, aggressive, or indolent B-cell lymphomas, mantle-cell lymphomas, peripheral T-cell lymphomas, or Hodgkin's lymphomas who were treated with the modified DHAP (mDHAP) regimen (dexamethasone 40 mg 15 min-i...
September 28, 2019: Journal of Cancer Research and Clinical Oncology
Rahim Heidari Barchi Nezhad, Fateme Asadi, Seyyed Meysam Abtahi Froushani, Gholamhossein Hassanshahi, Ayat Kaeidi, Soudeh Khanamani Falahati-Pour, Zahra Hashemi, Mohammad Reza Mirzaei
The present study was conducted aimed at exploring the modulatory effects of 17-b estradiol (17-bED) on mesenchymal stem cells (MSCs) in the EAE (experimental autoimmune encephalomyelitis) animal model of multiple sclerosis (MS). Following the isolation of bone marrow-derived MSCs from the bilateral femurs and tibias of the male Wistar rats, the cells were harvested and cultured in the presence of 100 nM 17-bED for 24 h. EAE was induced in male Wistar rats (8-12 weeks old) using guinea pig spinal cord homogenate, in combination with the complete Freund's adjuvant...
September 25, 2019: Molecular Biology Reports
Hakan Goker, Rafiye Ciftciler, Haluk Demiroglu, Mehmet Turgut, Nilgun Sayınalp, I C Haznedaroglu, Mufide Okay, Fatma Tekin, Yahya Buyukasık
BACKGROUND AND AIM: High-dose chemotherapy followed by autologous stem cell transplantation (ASCT) has been considered the standard of treatment care for patients with multiple myeloma (MM). Insufficient mobilization and harvest of peripheral stem cells can be a major obstacle for performing ASCT. This is resulting in a lacking opportunity of cure in patients with MM. The aim of this study was to evaluate the factors which influence mobilization failure in patients with MM. MATERIALS AND METHODS: This study has been performed in a retrospective manner...
August 8, 2019: Transfusion and Apheresis Science
Daniel Kraft, Emily Walck, Antonio Carrasco, Michael Crocker, Lin Song, Marc Long, Maia Mosse, Bilal Nadeem, Galym Imanbyev, Agnieszka Czechowicz, Michael McCullough
Bone marrow (BM) is a rich source of hematopoietic stem cells (HSC), mesenchymal stem cells (MSC) and other important stem/progenitor cells. It is the traditional source of cells utilized in hematopoietic cell transplantation (HCT), which is a proven curative treatment for many blood and immune diseases. BM-derived cells have also been shown to have other diverse clinical uses and are increasingly being utilized in orthopedic, regenerative medicine, and gene therapy applications. Traditional methods for harvesting BM are crude, tedious, time-consuming and expensive; requiring multiple bone punctures under general anesthesia with serial small volume aspirates often diluted with peripheral blood...
September 3, 2019: Biology of Blood and Marrow Transplantation
Matthias Schlund, Romain Nicot, Arnaud Depeyre, Juma Alkasbi, Joël Ferri
INTRODUCTION: Currently, vascularized autologous bone transplantation is considered the gold standard for large mandibular continuity defect reconstruction. Donor site morbidity is a major concern. Therefore, bone tissue engineering (BTE) seems to be the ideal solution. Fresh-frozen bone allograft is the closest material to autologous bone. The purpose of this clinical report is to show a new technique of large mandibular continuity defect reconstruction using a fresh-frozen humeral allograft seeded with autologous iliac bone marrow aspirate and vascularized with a radial forearm flap...
September 4, 2019: Journal of Craniofacial Surgery
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