Jie Xia, Jiangmei Wang, Liyang Ying, Ruoqiong Huang, Kai Zhang, Ruoyang Zhang, Wenqi Tang, Qi Xu, Dengming Lai, Yan Zhang, Yaoqin Hu, Xiaodie Zhang, Ruoxi Zang, Jiajie Fan, Qiang Shu, Jianguo Xu
SARS-CoV-2 nucleocapsid protein (N-protein) rises early in body fluids during infection and has recently been identified as a direct inducer for lung injury. However, the signal mechanism of N-protein in lung inflammatory response remains poorly understood. The goal of this study was to determine whether receptor for advanced glycation endproducts (RAGE) participated in N-protein-induced acute lung injury. The binding between N-protein and RAGE was examined via assays for protein-protein interaction. To determine the signaling mechanism in vitro, cells were treated with recombinant N-protein and assayed for the activation of RAGE/mitogen-activated protein kinase/NF-ĸB pathway...
July 21, 2023: American Journal of Respiratory Cell and Molecular Biology