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JOURNAL ARTICLE
I A Ishina, M Y Zakharova, I N Kurbatskaia, A E Mamedov, A A Belogurov, Yu P Rubtsov, A G Gabibov
Study of CD4+ T cell response and T cell receptor (TCR) specificity is crucial for understanding etiology of immune-mediated diseases and developing targeted therapies. However, solubility, accessibility, and stability of synthetic antigenic peptides used in T cell assays may be a critical point in such studies. Here we present a T cell activation reporter system using recombinant proteins containing antigenic epitopes fused with bacterial thioredoxin (trx-peptides) and obtained by bacterial expression. We report that co-incubation of CD4+ HA1...
May 3, 2024: Doklady. Biochemistry and Biophysics
#2
JOURNAL ARTICLE
Sheeba Zarin, Mohd Shariq, Nilisha Rastogi, Yashika Ahuja, P Manjunath, Anwar Alam, Seyed Ehtesham Hasnain, Nasreen Zafar Ehtesham
Nitrogen metabolism of M. tuberculosis is critical for its survival in infected host cells. M. tuberculosis has evolved sophisticated strategies to switch between de novo synthesis and uptake of various amino acids from host cells for metabolic demands. Pyridoxal phosphate-dependent histidinol phosphate aminotransferase-HspAT enzyme is critically required for histidine biosynthesis. HspAT is involved in metabolic synthesis of histidine, phenylalanine, tyrosine, tryptophan, and novobiocin. We showed that M. tuberculosis Rv2231c is a conserved enzyme with HspAT activity...
May 2, 2024: Cellular and Molecular Life Sciences: CMLS
#3
JOURNAL ARTICLE
Zaven A Karalyan, Susanna A Ghonyan, Davit A Poghosyan, Lina H Hakobyan, Hranush R Avagyan, Aida S Avetisyan, Liana O Abroyan, Arpine A Poghosyan, Sona A Hakobyan, Gayane P Manukyan
BACKGROUND: The African swine fever (ASF) virus (ASFV) and ASF-like viral sequences were identified in human samples and sewage as well as in different water environments. Pigs regularly experience infections by the ASFV. The considerable stability of the virus in the environment suggests that there is ongoing and long-term contact between humans and the ASFV. However, humans exhibit resistance to the ASFV, and the decisive factor in developing infection in the body is most likely the reaction of target macrophages to the virus...
April 23, 2024: Frontiers in Bioscience (Landmark Edition)
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JOURNAL ARTICLE
Jingyun Luan, Cynthia Truong, Aleksandra Vuchkovska, Weijie Guo, Jennifer Good, Bijun Liu, Audrey Gang, Nicole Infarinato, Katherine Stewart, Lisa Polak, Hilda Amalia Pasolli, Emma Andretta, Alexander Y Rudensky, Elaine Fuchs, Yuxuan Miao
Following tissue damage, epithelial stem cells (SCs) are mobilized to enter the wound, where they confront harsh inflammatory environments that can impede their ability to repair the injury. Here, we investigated the mechanisms that protect skin SCs within this inflammatory environment. Characterization of gene expression profiles of hair follicle SCs (HFSCs) that migrated into the wound site revealed activation of an immune-modulatory program, including expression of CD80, major histocompatibility complex class II (MHCII), and CXC motif chemokine ligand 5 (CXCL5)...
April 22, 2024: Immunity
#5
JOURNAL ARTICLE
Sena Kim, Peter Ruminski, Megh Singh, Karl Staser, Kidist Ashami, Julie Ritchey, Sora Lim, John F DiPersio, Jaebok Choi
Allogeneic hematopoietic cell transplantation (allo-HCT) is a highly effective, well-established treatment for patients with various hematologic malignancies and non-malignant diseases. The therapeutic benefits of allo-HCT are mediated by alloreactive T cells in donor grafts. However, there is a significant risk of graft-versus-host disease (GvHD), in which the donor T cells recognize recipient cells as foreign and attack healthy organs in addition to malignancies. We previously demonstrated that targeting JAK1/JAK2, mediators of interferon-gamma receptor (IFNGR) and IL-6 receptor signaling, in donor T cells using baricitinib and ruxolitinib results in a significant reduction in GvHD after allo-HCT...
April 16, 2024: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
#6
JOURNAL ARTICLE
Silvia Lopa, Francesca Libonati, Katia Mareschi, Giuseppe Talò, Stefania Brambilla, Vincenzo Raffo, Luciana Labanca, Luigi Zagra, Matteo Moretti, Laura de Girolamo, Alessandra Colombini
Macrophage-based co-cultures are used to test the immunomodulatory function of candidate cells for clinical use. This study aimed to characterize a macrophage polarization model using human platelet lysate (hPL) as a GMP-compliant alternative to Fetal Bovine Serum (FBS). Primary human monocytes were differentiated into unpolarized (M0) or polarized (M1, M2a, and M2c) macrophages in an hPL- or FBS-based medium. The protein secretion profiles and expression of phenotypic markers (CD80 for M1, CD206 for M2a, and CD163 for M2c) were analyzed...
April 9, 2024: Biomedicines
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JOURNAL ARTICLE
Minghui Dong, Ziying Yang, Qian Gao, Qingyuan Deng, Le Li, Hongmei Chen
2,2',4,4'-Tetrabrominated biphenyl ether (BDE-47) is a polybrominated diphenyl ether (PBDE) homologue that is ubiquitous in biological samples and highly toxic to humans and other organisms. Prior research has confirmed that BDE-47 can induce oxidative damage in RAW264.7 cells, resulting in apoptosis and impaired immune function. The current study mainly focused on how Isoliquiritigenin (ISL) and Licochalcone B (LCB) might protect against BDE-47's immunotoxic effects on RAW264.7 cells. The results show that ISL and LCB could increase phagocytosis, increase the production of MHC-II , and decrease the production of inflammatory factors (TNF-α, IL-6, and IL-1β) and co-stimulatory factors ( CD40 , CD80 , and CD86 ), alleviating the immune function impairment caused by BDE-47...
April 10, 2024: Antioxidants (Basel, Switzerland)
#8
JOURNAL ARTICLE
Xiangyu Hu, Chunmiao Hu, Liting Liao, Huimin Zhang, Xingmeng Xu, Jie Xiang, Guotao Lu, Xiaoqin Jia, Hongwei Xu, Weijuan Gong
Isoliquiritigenin (ISL) is a chalcone-type flavonoid derived from the root of licorice with antioxidant, anti-inflammatory, anti-tumour and neuroprotective properties. ISL has been proven to downregulate the productions of IL-1β, TNF-α and IL-6 by macrophages. However, detailed molecular mechanisms of this modulation remain elusive. Here, ISL suppressed Syk phosphorylation and CD80, CD86, IL-1β, TNF-α and IL-6 expressions in lipopolysaccharide-stimulated macrophages ex vivo. ApoC3-transgenic (ApoC3TG ) mice had more activated macrophages...
April 26, 2024: Scandinavian Journal of Immunology
#9
JOURNAL ARTICLE
Yan Chen, Tingjun Tao, Zhaoxin Liang, Xiangnong Chen, Ya'nan Xu, Tangtang Zhang, Dunrong Zhou
OBJECTIVE: Dihydroartemisinin (DHA) plays a very important role in various diseases. However, the precise involvement of DHA in systemic lupus erythematosus (SLE), relation to the equilibrium between M1 and M2 cells, remains uncertain. Therefore, we aimed to investigate the role of DHA in SLE and its effect on the M1/M2 cells balance. METHODS: SLE mice model was established by pristane induction. Flow cytometry was employed to measure the abundance of M1 and M2 cells within the peripheral blood of individuals diagnosed with SLE...
April 25, 2024: Molecular Immunology
#10
JOURNAL ARTICLE
JinYing Xiang, Bo Liu, Yan Li, Yinying Ren, Yuehan Li, Mi Zhou, Jinyue Yu, Zhengxiu Luo, Enmei Liu, Zhou Fu, Fengxia Ding
OBJECTIVE: Asthma stands as one of the most prevalent chronic respiratory conditions in children, with its pathogenesis tied to the actived antigen presentation by dendritic cells (DCs) and the imbalance within T cell subgroups. This study seeks to investigate the role of the transcription factor EB (TFEB) in modulating the antigen presentation process of DCs and its impact on the differentiation of T cell subgroups. METHODS: Bone marrow dendritic cells (BMDCs) were activated using house dust mites (HDM) and underwent RNA sequencing (RNA-seq) to pinpoint differentially expressed genes...
April 25, 2024: Respiratory Research
#11
JOURNAL ARTICLE
Jonathan Sun, Enric Esplugues, Alicia Bort, Magdalena P Cardelo, Inmaculada Ruz-Maldonado, Pablo Fernández-Tussy, Clara Wong, Hehe Wang, Iwao Ojima, Martin Kaczocha, Rachel Perry, Yajaira Suárez, Carlos Fernández-Hernando
Due to the rise in overnutrition, the incidence of obesity-induced hepatocellular carcinoma (HCC) will continue to escalate; however, our understanding of the obesity to HCC developmental axis is limited. We constructed a single-cell atlas to interrogate the dynamic transcriptomic changes during hepatocarcinogenesis in mice. Here we identify fatty acid binding protein 5 (FABP5) as a driver of obesity-induced HCC. Analysis of transformed cells reveals that FABP5 inhibition and silencing predispose cancer cells to lipid peroxidation and ferroptosis-induced cell death...
April 25, 2024: Nature metabolism
#12
JOURNAL ARTICLE
Chen Yuan, Yidan Lin, Yawen Wang, Yanan Zhang, Xue Zhao, Hongxing Yuan, Tanqing Li, Qinye Song
Porcine epidemic diarrhea virus (PEDV) is a devastating pathogen of acute- gastrointestinal infectious diseases, which can cause vomiting, diarrhea, dehydration and high morbidity and mortality among neonatal piglets. Humoral immunity plays a vital role in the host anti-PEDV infection process, but the mechanism of PEDV-induced B-cell immune response remains unknown. In this study, the effects of PEDV infection on CD21+ B cell activation were systematically analyzed through animal experiments. Enzyme-linked immunosorbent assays (ELISA) revealed that low levels of serum-specific IgA, IgM, or IgG were detected in piglets after PEDV infection, respectively...
April 13, 2024: Veterinary Microbiology
#13
JOURNAL ARTICLE
Timothy Arthur Chandos Snow, Alessia V Waller, Richard Loye, Francis Ryckaert, Antonio Cesar, Naveed Saleem, Rudra Roy, John Whittle, Ahmed Al-Hindawi, Abhishek Das, Mervyn Singer, David Brealey, Nishkantha Arulkumaran
BACKGROUND: Post-operative infections are a common cause of morbidity following major surgery. Little is understood about how major surgery perturbs immune function leading to heightened risk of subsequent infection. Through analysis of paired blood samples obtained immediately before and 24 h following surgery, we evaluated changes in circulating immune cell phenotype and function across the first 24 h, to identify early immune changes associated with subsequent infection. METHODS: We conducted a prospective observational study of adult patients undergoing major elective gastrointestinal, gynecological, or maxillofacial surgery requiring planned admission to the post-anesthetic care unit...
2024: Frontiers in Immunology
#14
JOURNAL ARTICLE
Patrizia Leone, Eleonora Malerba, Marcella Prete, Antonio Giovanni Solimando, Giorgio Alberto Croci, Paolo Ditonno, Marco Tucci, Nicola Susca, Afshin Derakhshani, Antoine Dufour, Valli De Re, Nicola Silvestris, Vito Racanelli
BACKGROUND: Activation of CD28 on multiple myeloma (MM) plasma cells, by binding to CD80 and CD86 on dendritic cells, decreases proteasome subunit expression in the tumor cells and thereby helps them evade being killed by CD8+ T cells. Understanding how CD28 activation leads to proteasome subunit downregulation is needed to design new MM therapies. METHODS: This study investigates the molecular pathway downstream of CD28 activation, using an in vitro model consisting of myeloma cell lines stimulated with anti-CD28-coated beads...
April 23, 2024: Biomarker Research
#15
REVIEW
Marlena Typiak, Dorota Żurawa-Janicka
The cells presented in this work are not classified as cells that make up the immune system. They, however, present functions and molecules, which are characteristic of immune cells. These characteristic functions are, for example, sensing threat, performing phagocytosis, presentation of foreign antigens, cytokine release or enhancing immune memory. The enlisted immune response mechanisms are carried out by the possession of molecules such as Toll-like receptors, receptors for the Fc fragment of IgG, major histocompatibility complex class II molecules, costimulatory CD80/CD86 proteins and molecules needed for NLRP3 (NOD-like family pyrin domain containing 3) inflammasome activation...
April 22, 2024: Immunology and Cell Biology
#16
JOURNAL ARTICLE
Ottavia De Simoni, Melania Scarpa, Ignazio Castagliuolo, Astghik Stepanyan, Imerio Angriman, Andromachi Kotsafti, Camilla Nacci, Federico Scognamiglio, Silvia Negro, Antonella D'Angelo, Valentina Chiminazzo, Gianluca Businello, Cesare Ruffolo, Roberta Salmaso, Boris Franzato, Mario Gruppo, Pierluigi Pilati, Antonio Scapinello, Anna Pozza, Tommaso Stecca, Marco Massani, Ivana Cataldo, Stefano Brignola, Angelo Paolo Dei Tos, Carlotta Ceccon, Vincenza Guzzardo, Chiara Vignotto, Luca Facci, Isacco Maretto, Marco Agostini, Francesco Marchegiani, Giulia Becherucci, Maurizio Zizzo, Giovanni Bordignon, Roberto Merenda, Giovanni Pirozzolo, Alfonso Recordare, Giulia Pozza, Mario Godina, Isabella Mondi, Daunia Verdi, Corrado Da Lio, Licia Laurino, Luca Saadeh, Giorgio Rivella, Silvio Guerriero, Chiara Romiti, Giuseppe Portale, Chiara Cipollari, Ylenia Camilla Spolverato, Giulia Noaro, Roberto Cola, Salvatore Candioli, Laura Gavagna, Fabio Ricagna, Monica Ortenzi, Mario Guerrieri, Giovanni Tagliente, Monica Tomassi, Umberto Tedeschi, Beatrice Salmaso, Gianluca Buzzi, Dario Parini, Daniela Prando, Matteo Zuin, Francesca Bergamo, Vittorina Zagonel, Andrea Porzionato, Francesco Cavallin, Barbara Di Camillo, Loretta Di Cristoforo, Quoc Riccardo Bao, Salvatore Pucciarelli, Romeo Bardini, Gaya Spolverato, Matteo Fassan, Marco Scarpa
BACKGROUND: Long-term daily use of aspirin reduces incidence and mortality due to colorectal cancer (CRC). This study aimed to analyze the effect of aspirin on the tumor microenvironment, systemic immunity, and on the healthy mucosa surrounding cancer. METHODS: Patients with a diagnosis of CRC operated on from 2015 to 2019 were retrospectively analyzed (METACCRE cohort). Expression of mRNA of immune surveillance-related genes (PD-L1, CD80, CD86, HLA I, and HLA II) in CRC primary cells treated with aspirin were extracted from Gene Expression Omnibus-deposited public database (GSE76583)...
April 22, 2024: Cancer
#17
JOURNAL ARTICLE
Alessia Ricci, Susi Zara, Fabrizio Carta, Valentina Di Valerio, Silvia Sancilio, Amelia Cataldi, Silvia Selleri, Claudiu T Supuran, Simone Carradori, Marialucia Gallorini
Macrophage polarization towards the M1 phenotype under bacterial product-related exposure (LPS) requires a rapid change in gene expression patterns and cytokine production along with a metabolic rewiring. Metabolic pathways and redox reactions are such tightly connected, giving rise to an area of research referred to as immunometabolism. A role in this context has been paid to the master redox-sensitive regulator Nuclear factor erythroid 2-related factor 2 (Nrf2) and to the 5'-ectonucleotidase CD73, a marker related to macrophage metabolism rearrangement under pro-inflammatory conditions...
April 20, 2024: Molecular Immunology
#18
JOURNAL ARTICLE
Thansita Bhunyakarnjanarat, Jiradej Makjaroen, Wilasinee Saisorn, Kankorn Hirunsap, Jidapond Chiewchengchol, Patcharee Ritprajak, Asada Leelahavanichkul
BACKGROUND: Although allergy might be another factor that exacerbates lupus as demonstrated by several epidemiologic studies, the direct correlation between lupus activities and allergy is still in question. OBJECTIVE: To explore the correlation between allergic reaction and lupus activities. METHODS: The allergic asthma model using ovalbumin (OVA) administration in wildtype (WT) and Fc gamma receptor IIb deficient (FcgRIIb-/-) mice (a lupus-prone model) together with in vitro experiments on bone marrow-derived dendritic cells (DCs) were performed...
April 20, 2024: Asian Pacific Journal of Allergy and Immunology
#19
JOURNAL ARTICLE
Haiqin Liu, Shuo Yan, Ruiming Yang, Caidi Huang, Kangyue Guo, Shi Wang, Yunmei Huang, Dongyi Shen, Ying Lin, Zhiyun Cao, Hangyan Zhong, Jiumao Lin, Xuzheng Chen
To investigate the effect of Jiedu Xiaozheng Yin (JXY) on the polarization of macrophages in colitis-associated colon cancer (CAC). An orthotopic model of CAC was established to monitor changes in the pathological state of mice. Colon length, number of colon tumors were recorded, and indices for liver, spleen, and thymus were calculated. Hematoxylin and eosin (H&E) staining was employed to observe intestinal mucosal injury and tumor formation. Immunohistochemistry (IHC) staining was utilized to investigate the effect of JXY on M1 and M2 polarization of macrophages in the colonic mucosa of CAC mice...
2024: Integrative Cancer Therapies
#20
JOURNAL ARTICLE
Roos van Schuijlenburg, Samaneh Azargoshasb, Clarize M de Korne, Jeroen C Sijtsma, Sascha Bezemer, Alwin J van der Ham, Els Baalbergen, Fiona Geurten, Laura M de Bes-Roeleveld, Severine C Chevalley-Maurel, Matthias N van Oosterom, Fijs W B van Leeuwen, Blandine Franke-Fayard, Meta Roestenberg
BACKGROUND: Sporozoites (SPZ), the infective form of Plasmodium falciparum malaria, can be inoculated into the human host skin by Anopheline mosquitoes. These SPZ migrate at approximately 1 µm/s to find a blood vessel and travel to the liver where they infect hepatocytes and multiply. In the skin they are still low in number (50-100 SPZ) and vulnerable to immune attack by antibodies and skin macrophages. This is why whole SPZ and SPZ proteins are used as the basis for most malaria vaccines currently deployed and undergoing late clinical testing...
April 19, 2024: Malaria Journal
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