Meili Zhao, Yanchang Zhao, Zhenyu Yang, Feng Ming, Jian Li, Demin Kong, Yu Wang, Peng Chen, Meina Wang, Zhicai Wang
The sesquiterpene alkaloid dendrobine, widely recognized as the main active compound and a quality control standard of medicinal orchids in the Chinese Pharmacopoeia, demonstrates diverse biological functions. In this study, we engineered Dendrobium catenatum as a chassis plant for the production of dendrobine through the screening and pyramiding of key biosynthesis genes. Initially, previously predicted upstream key genes in the methyl-D-erythritol 4-phosphate (MEP) pathway for dendrobine synthesis, including 4-(Cytidine 5'-Diphospho)-2-C-Methyl-d-Erythritol Kinase ( CMK ), 1-Deoxy-d-Xylulose 5-Phosphate Reductoisomerase ( DXR ), 2-C-Methyl-d-Erythritol 4-Phosphate Cytidylyltransferase ( MCT ), and Strictosidine Synthase 1 ( STR1 ), and a few downstream post-modification genes, including Cytochrome P450 94C1 ( CYP94C1 ), Branched-Chain-Amino-Acid Aminotransferase 2 ( BCAT2 ), and Methyltransferase-like Protein 23 ( METTL23 ), were chosen due to their deduced roles in enhancing dendrobine production...
December 28, 2023: International Journal of Molecular Sciences