Chaoqun Yuan, Tian Tian, Jian Sun, Menglu Hu, Xusheng Wang, Erhu Xiong, Meng Cheng, Yijuan Bao, Wei Lin, Jieming Jiang, Chengwei Yang, Qian Chen, Huang Zhang, Heng Wang, Xiran Wang, Xianbo Deng, Xiaoping Liao, Yahong Liu, Zhang Wang, Guihong Zhang, Xiaoming Zhou
Gold-nanoparticles-based colorimetric assay is an attractive detection format, but is limited by the tedious and ineffective posthybridization manipulations for genomic analysis. Here, we present a new design for a colorimetric gene-sensing platform based on the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system. In this strategy, programmable recognition of DNA by Cas12a/crRNA and RNA by Cas13a/crRNA with a complementary target activates the trans -ssDNA or -ssRNA cleavage. Target-induced trans -ssDNA or ssRNA cleavage triggers an aggregation behavior change for the designed AuNPs-DNA probes pair, enabling the completion of naked-eye gene detection (transgenic rice, African swine fever virus, and miRNAs as the models) within 1 h...
March 3, 2020: Analytical Chemistry