Takafumi Nakano, Seiichiro Takao, Katsushi Dairaku, Naoki Uno, Siew-Kee Amanda Low, Masahiro Hashimoto, Yasuo Tsuda, Yuichi Hisamatsu, Takeo Toshima, Yusuke Yonemura, Takaaki Masuda, Ken Eto, Toru Ikegami, Yosuke Fukunaga, Atsushi Niida, Satoshi Nagayama, Koshi Mimori
Considering the cost and invasiveness of monitoring postoperative minimal residual disease (MRD) of colorectal cancer (CRC) after adjuvant chemoradiotherapy (ACT), we developed a favorable approach based on methylated circulating tumor DNA to detect MRD after radical resection. Analyzing the public database, we identified the methylated promoter regions of the genes FGD5, GPC6, and MSC. Using digital polymerase chain reaction (dPCR), we termed the "amplicon of methylated sites using a specific enzyme" assay as "AMUSE...
March 26, 2024: Cancer Science