Sarah Abdullah, Michael Ghio, Aaron Cotton-Betteridge, Aditya Vinjamuri, Robert Drury, Jacob Packer, Oguz Aras, Jessica Friedman, Mardeen Karim, David Engelhardt, Emma Kosowski, Kelby Duong, Farhana Shaheen, Patrick R McGrew, Charles T Harris, Robert Reily, Mimi Sammarco, Partha K Chandra, Derek Pociask, Jay Kolls, Prasad V Katakam, Alison Smith, Sharven Taghavi, Juan Duchesne, Olan Jackson-Weaver
Acute hemorrhage commonly leads to coagulopathy and organ dysfunction or failure. Recent evidence suggests that damage to the endothelial glycocalyx contributes to these adverse outcomes. The physiological events mediating acute glycocalyx shedding are undefined, however. Here, we show that succinate accumulation within endothelial cells drives glycocalyx degradation through a membrane reorganization-mediated mechanism. We investigated this mechanism in a cultured endothelial cell hypoxia-reoxygenation model, in a rat model of hemorrhage, and in trauma patient plasma samples...
June 16, 2023: Science Advances