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Single cell westernblot

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https://read.qxmd.com/read/28054314/turning-back-the-wheel-inducing-mesenchymal-to-epithelial-transition-via-wilms-tumor-1-knockdown-in-human-mesothelioma-cell-lines-to-influence-proliferation-invasiveness-and-chemotaxis
#1
Till Plönes, Mitja Fischer, Kerstin Höhne, Hiromi Sato, Joachim Müller-Quernheim, Gernot Zissel
Malignant pleural mesothelioma (MPM) is a highly aggressive tumor that arises from the surface of the pleura and is associated with a history of asbestos exposure. The tumor is characterized by a strong local invasiveness and a poor response to any single modality therapy. Therefore clinical outcome of patients with MPM is poor and median survival time of untreated patients with MPM is 7 months from initial diagnosis. The Wilms Tumor Protein 1 (WT1) is a transcription factor which is highly expressed by MPM and is involved in cellular development and survival...
October 2017: Pathology Oncology Research: POR
https://read.qxmd.com/read/22145043/scavenger-receptor-mediates-systemic-rna-interference-in-ticks
#2
Kyaw Min Aung, Damdinsuren Boldbaatar, Rika Umemiya-Shirafuji, Min Liao, Xuan Xuenan, Hiroshi Suzuki, Remil Linggatong Galay, Tetsuya Tanaka, Kozo Fujisaki
RNA interference is an efficient method to silence gene and protein expressions. Here, the class B scavenger receptor CD36 (SRB) mediated the uptake of exogenous dsRNAs in the induction of the RNAi responses in ticks. Unfed female Haemaphysalis longicornis ticks were injected with a single or a combination of H. longicornis SRB (HlSRB) dsRNA, vitellogenin-1 (HlVg-1) dsRNA, and vitellogenin receptor (HlVgR) dsRNA. We found that specific and systemic silencing of the HlSRB, HlVg-1, and HlVgR genes was achieved in ticks injected with a single dsRNA of HlSRB, HlVg-1, and HlVgR...
2011: PloS One
https://read.qxmd.com/read/17062949/-effect-of-chronic-stress-on-pka-and-p-creb-expression-in-hippocampus-of-rats-and-the-antagonism-of-antidepressors
#3
Zhe Wang, Sui-yu Hu, De-liang Lei, Wei-xi Song
OBJECTIVE: To observe the effect of chronic unpredicted sequence of mild stress on the expression of cAMP-dependent protein kinase A(PKA) and phosphorylated cAMP-responsive element binding protein (P-CREB) in hippocampus of rats and the antagonism of antidepressors (fluoxetine). METHODS: Thirty-six male Sprague Dawley rats were randomly and equally allocated to 3 groups: A normal control group, a model group, and a fluoxetine group. All rats except the control group were singly housed and exposed to an unpredicted sequence of mild stressors...
October 2006: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://read.qxmd.com/read/7826223/-a-study-on-subpopulation-of-helper-t-cells-in-chronic-inflammatory-demyelinating-polyneuropathy
#4
A Inoue, T Iwahashi, C S Koh, N Yanagisawa
Chronic inflammatory demyelinating polyneuropathy (CIDP) is thought to be an inflammatory autoimmune disease against peripheral myelin. Recently several animal models have suggested that preferential activation of Th1 cell response is central to the pathogenesis of this disease. We studied the function of CD4 positive T cell subsets, Th1 cells and Th2 cells by analyzing their representative secreting cytokines. Th1 cells secrete Interferon gamma (IFH gamma) and Th2 cells secrete Interleukin 4 (IL4). Using enzyme linked immunospot (ELISPOT) assay, a very sensitive single cell analysis system, we enumerated IFN gamma and IL4 secreting peripheral blood lymphocytes in patients with CIDP...
October 1994: Arerugī, [Allergy]
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