Rubal Singla, Abhishek Mishra, Rupa Joshi, Sonali Jha, Amit Raj Sharma, Sujata Upadhyay, Phulen Sarma, Ajay Prakash, Bikash Medhi
Coronaviruses are a large family of viruses that are known to infect both humans and animals. However, the evidence of inter-transmission of coronavirus between humans and companion animals is still a debatable issue. There is substantial evidence that the virus outbreak is fueled by zoonotic transmission because this new virus belongs to the same family of viruses as SARS-CoV associated with civet cats, and MERS-CoV associated with dromedary camels. While the whole world is investigating the possibility about the transmission of this virus, the transmission among humans is established, but the interface between humans and animals is not much evident...
September 14, 2020: Veterinary Research Communications
Lakshmi Vasudevan, Christophe P Stove
The Mu opioid receptor (MOR) has been the subject of intense research over the past decades, especially in the field of analgesic therapeutics. It is the primary target for both clinical and recreational opioids. Recently, camelid-derived nanobodies have received significant attention due to their applicability in stabilizing the crystal structure of activated MOR, via specific recognition of and binding to the active receptor conformation. In the present study, we developed and applied a novel bio-assay to monitor MOR activation, utilizing intracellular expression of one such nanobody, Nb39...
September 14, 2020: Analytical and Bioanalytical Chemistry
Stacy Chapman, Karen Ballinger, Ming-Ka Chan, Camelia Stefanovici, Katya Rozovsky, April Boyd, Magimairajan I Vanan
Arterial occlusive disease of the limb is very rare in children. Buerger's disease (BD) is a nonatherosclerotic, segmental inflammatory arteritis affecting the small and medium-sized vessels of the extremities. We report BD in a 16-year-old male presenting with arterial insufficiency of left foot and history of smoking cigarettes and cannabis for 2 years. BD was diagnosed based on history of smoking in combination with clinical, laboratory, and radiologic findings. Pediatric hemato-oncologists should consider BD in the differential diagnosis in adolescents who smoke cigarettes and/or cannabis and present with vascular insufficiency of the hands and/or feet...
September 9, 2020: Journal of Pediatric Hematology/oncology
John D Gilbert, Roger W Byard
Camels are ungulates of the genus Camelus and have been used for centuries in parts of Asia and Africa for transport and sustenance. Handling of camels is not without its dangers, and 4 cases from South Australia are reported with a review of lethal camel-related issues. Case 1 is a 56-year-old man who died of multiple blunt force injuries after he had attempted to move a 7-year-old female dromedary (Camelus dromedarius). Case 2 is a 65-year-old woman who was crushed by 1 or more camels that she had been training...
September 8, 2020: American Journal of Forensic Medicine and Pathology
Richard Broughton, Douglas R Tocher, Mónica B Betancor
Lipidomics methodologies traditionally utilize either reverse phase- or hydrophilic interaction liquid chromatography-type separations; however, supercritical fluid chromatography can offer a rapid normal phase type separation while reducing the dependence on organic solvents. However, normal phase type lipid separations typically lack pronounced intraclass separation, which is problematic for complex lipidomes containing very-long-chain polyunsaturated fatty acids, especially those from genetically modified organisms...
September 8, 2020: ACS Omega
Lidiia S Samarina, Lyudmila S Malyukova, Alexander M Efremov, Taisiya A Simonyan, Alexandra O Matskiv, Natalia G Koninskaya, Ruslan S Rakhmangulov, Maya V Gvasaliya, Valentina I Malyarovskaya, Alexey V Ryndin, Yuriy L Orlov, Wei Tong, Magda-Viola Hanke
Background: Cold and frost are two serious factors limiting the yield of many crops worldwide, including the tea plant (Camellia sinensis (L.) Kuntze). The acclimatization of tea plant from tropical to temperate climate regions resulted in unique germplasm in the North-Western Caucasus with extremely frost-tolerant genotypes. Methods: The aim of the current research was to evaluate the physiological, biochemical and genetic responses of tolerant and sensitive tea cultivars exposed to cold (0 to +2 °C for 7 days) and frost (-6 to -8 °C for 5 days)...
2020: PeerJ
Roua A Iorga, Nicolae Bacalbasa, Ovidiu G Bratu, Florentina Ionita Radu, Camelia C Diaconu
Chronic hepatitis C virus (HCV) infection represents a systemic disease, with a natural progression to hepatic steatosis, fibrosis, and finally, cirrhosis, with an increased risk of hepatocellular carcinoma. Besides the hepatic alterations, the systemic manifestations of chronic HCV infection, such as endothelial dysfunction and atherosclerosis, oxidative stress, insulin resistance, immunological alterations, are nowadays recognized as cardiovascular risk factors. Hepatitis C is associated with insulin resistance and increased risk for type 2 diabetes mellitus, carotid atherosclerosis and stroke, coronary artery disease and chronic heart failure, with a significant impact on the mortality and morbidity...
2020: American Journal of Cardiovascular Disease
Ruokun Yi, Yuxuan Wei, Fang Tan, Jianfei Mu, Xingyao Long, Yanni Pan, Weiwei Liu, Xin Zhao
Shoumei is a kind of white tea (slightly fermented Camellia sinensis ) that is rich in polyphenols. In this study, polyphenols were extracted from Shoumei. High-performance liquid chromatography (HPLC) showed that the polyphenols included mainly gallic acid, catechin, hyperoside, and sulfuretin. In an in vitro experiment, H2 O2 was used to induce oxidative damage in human normal hepatic L-02 cells. In an animal experiment, CCl4 was used to induce liver injury. The in vitro results showed that Shoumei polyphenols inhibited oxidative damage in normal hepatic L-02 cells, and the in vivo results showed that the polyphenols effectively reduced liver index values in mice with liver injury...
2020: Oxidative Medicine and Cellular Longevity
Hemn Othman, Heshu Rahman, Syam Mohan, Sadat Aziz, Hardi Marif, Dianne Ford, Nozlena Abdulsamad, Kawa Amin, Rasedee Abdullah
This study investigated the in vivo antileukemic activity of palladium nanoparticles (Pd@W.tea-NPs) mediated by white tea extract in a murine model. The cell viability effect of Pd@W.tea-NPs, "blank" Pd nanoparticles, and white tea extract alone was determined in murine leukemia WEHI-3B cells and normal mouse fibroblasts (3T3 cells). Apoptotic and cell cycle arrest effects of Pd@W.tea-NPs in WEHI-3B cells were evaluated. The effects of Pd@W.tea-NPs administered orally to leukemic mice at 50 and 100 mg/kg daily over 28 days were evaluated...
2020: Evidence-based Complementary and Alternative Medicine: ECAM
Ida Lager, Simon Jeppson, Anna-Lena Gippert, Ivo Feussner, Sten Stymne, Sofia Marmon
Camelina sativa is an emerging biotechnology oil crop. However, more information is needed regarding its innate lipid enzyme specificities. We have therefore characterized several triacylglycerol (TAG) producing enzymes by measuring in vitro substrate specificities using different combinations of acyl-acceptors (diacylglycerol, DAG) and donors. Specifically, C. sativa acyl-CoA:diacylglycerol acyltransferase (DGAT) 1 and 2 (which both use acyl-CoA as acyl donor) and phospholipid:diacylglycerol acyltransferase (PDAT, with phosphatidylcoline as acyl donor) were studied...
2020: Frontiers in Plant Science
Xiao Hai, Guo-Qiang Liu, Jian-Xing Luo, Yuan-Sheng Guo, Jun-Ping Qian, Mei Ya, Liang Guo
Authentication of dairy and meat products is important to ensure fair competition, consumer benefit, and food safety. The large difference in price between camel and cow milk may be an incentive to adulterate camel dairy products with cow-derived foodstuffs. However, no studies so far have used triplex real-time PCR with an endogenous control to identify camel and cow origins in dairy and meat products. In this study, we developed a triplex real-time PCR assay based on amplification of mitochondrial 12S ribosomal DNA for the authentication of camel-derived dairy and meat products...
September 10, 2020: Journal of Dairy Science
E Hysa, A Sobrero, D Camellino, F Rumi, G Carrara, M Cutolo, C A Scirè, M A Cimmino
OBJECTIVES: Studies on the seasonality of onset of polymyalgia rheumatica (PMR) and giant cell arteritis (GCA) have shown conflicting results. The aim of this systematic literature review and meta-analysis is to determine from aggregated data whether there is a seasonal distribution for these diseases. METHODS: A literature search was performed using Pubmed Central and Embase scientific databases. The incidences per 6-month periods, season or month of onset, that were reported in the studies were summarised in tables considering the two diseases as separate conditions or together...
June 17, 2020: Seminars in Arthritis and Rheumatism
Giulia Elena Mandoli, Maria Concetta Pastore, Giovanni Benfari, Gianluigi Bisleri, Massimo Maccherini, Gianfranco Lisi, Paolo Cameli, Matteo Lisi, Aleksandr Dokollari, Chiara Carrucola, Mariangela Vigna, Gianfranco Montesi, Serafina Valente, Sergio Mondillo, Matteo Cameli
BACKGROUND: In patients with severe mitral regurgitation (MR), additional echocardiographic indices could be helpful to optimize surgical timing before irreversible left heart myocardial dysfunction has occurred. We investigated the correlation of left atrial (LA) strain by speckle tracking echocardiography with prognosis after mitral surgery for severe MR, and its association with LA fibrosis. METHOD: 71 patients with primary severe MR undergoing pre-operative echocardiographic assessment were initially enrolled...
September 10, 2020: International Journal of Cardiology
Soolmaz Moosavi, Camelia Rohani, Fariba Borhani, Mohammad Esmaeel Akbari
PURPOSE: Integrating spirituality into the patient care within a healthcare team, increases the ability to provide "holistic care" for cancer patients. The spiritual care experiences of different involved persons can be a guide for future planning. Therefore, this study aimed to explore the spiritual care experiences of hospitalized cancer patients, their family caregivers and healthcare team members in oncology settings. METHODS: This is a descriptive qualitative study which was conducted with 21 participants, who were selected by purposive sampling...
August 28, 2020: Explore: the Journal of Science and Healing
Bithika Mandal, Paromita Roy, Partha Mitra
The present work highlights biosynthesis of nano-sized heterometalic spinel ZnCo2 O4 particles using different green extracts as capping agent. In this work we have fabricated polygonal ZnCo2 O4 with Punica granatum peel extract, Camellia sinensis extract, Moringa oleifera leaf extract and green coffee beans extract in an effortless green pathway. Phase pure material synthesis was confirmed using XRD. Microstructural, morphological, compositional and optical characterisations has been carried out using TEM, FESEM, EDX, FTIR, photoluminescence and UV-Vis spectroscopy...
December 2020: Materials Science & Engineering. C, Materials for Biological Applications
Adrian Borhan, Dumitru-Daniel Herea, Daniel Gherca, Cristina Stavila, Anca-Emanuela Minuti, Marian Grigoras, Camelia Mihaela Danceanu, Luminita Labusca, George Stoian, Gabriel Ababei, Corneliu Stan, Nicoleta Lupu, Horia Chiriac
This work addresses current direction of the nanoparticles-based systems intended for cancer therapy by developing a newly-formulated innovative chemically-engineered anti-tumor composite consisting in a magnetic, fluorescent, lipophilic, and biologically-active carbon heterostructure capable by itself or through coupling with a chemotherapeutic agent to selectively induce tumor cell death. The anti-tumor compound was synthesized through a modified sol-gel method by addition of a low-cost molecule with recently proven anti-tumor properties which was combusted and flash-cooled along with magnetic iron oxides precursors at 250 °C...
December 2020: Materials Science & Engineering. C, Materials for Biological Applications
Sai Myo Thu Rein, Wai Wai Lwin, Sarun Tuntarawongsa, Thawatchai Phaechamud
The in situ forming system has attracted attention for periodontitis treatment owing to its sustainable drug release localisation at a periodontal pocket. Given its low aqueous solubility, beta-cyclodextrin (β-CD) may serve as a matrix former of solvent exchange-induced in situ forming gel (ISG) and microparticle (ISM). Meloxicam (Mex)-loaded-β-CD ISG and ISM were prepared using β-CD in dimethyl sulphoxide (ISG) as the internal phase and camellia oil comprising 5% glyceryl monostearate as the external phase (ISM)...
December 2020: Materials Science & Engineering. C, Materials for Biological Applications
Adrien Lemaire, Catalina Duran Garzon, Aurore Perrin, Olivier Habrylo, Pauline Trezel, Solène Bassard, Valérie Lefebvre, Olivier Van Wuytswinkel, Anaïs Guillaume, Corinne Pau-Roblot, Jérôme Pelloux
Rhamnogalaturonans I (RGI) pectins, which are a major component of the plant primary cell wall, can be recalcitrant to digestion by commercial enzymatic cocktails, in particular during fruit juice clarification process. To overcome these problems and get better insights into RGI degradation, three RGI degrading enzymes (RHG: Endo-rhamnogalacturonase; ABF: α-Arabinofuranosidases; GAN: Endo-β-1,4-galactanase) from Aspergillus aculeatinus were expressed in Pichia pastoris, purified and fully biochemically characterized...
November 15, 2020: Carbohydrate Polymers
Najeeb S Al-Zoreky, Faisal S Almathen
Bovine derived chymosin in rennet cannot coagulate camel milk (CAM). The study aimed at producing cheese curd from CAM using a recombinant camel chymosin. Pasteurized CAM was prepared for curdling using a recombinant camel chymosin (50 IMCU/ kg). CAM (pH 6.65) contained 2.83% Fat, 3.34% proteins and 9.11% non-fat solids. Physicochemical properties of soft cheese from cultured CAM were 51.89%, 15.62%, 20.21% and 2.47% for moisture, protein, fat and ash, respectively. Cultured CAM afforded higher cheese yield (8...
September 5, 2020: Food Chemistry
Asim Faraz, Carlos Iglesias Pastrana, Annamaria Passantino, Ayman Balla Mustafa, Abdul Waheed, Nasir Ali Tauqir, Muhammad Shahid Nabeel
Pakistani Marecha dromedaries, a distinctive breed not covered in the empirical data of the literature reviewed existing for thyroidal physiology in camels, are subjected to investigate thyroid function in pregnant multiparous (n = 22) and non-pregnant nor lactating (n = 22) females. The function of the thyroid gland in pubescent she-camels has evoked discrepancies among researchers exploring this topic in different breeds evolving in divergent environments. Despite season, age, sex, reproductive status, health disorders, and stress are reported as potential modulating factors, these research studies failed to find statistically significant results supporting their hypothesis...
September 11, 2020: Tropical Animal Health and Production
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