Ameliya Dickson, Elise Cooper, Lenu B Fakae, Bo Wang, Ka Lung Andrew Chan, Hany M Elsheikha
We examined the inhibitory effect of matcha green tea ( Camellia sinensis ) and epigallocatechin gallate (EGCg; the most abundant catechin in tea) on the vegetative growth and encystation of Acanthamoeba castellanii T4 genotype. The sulforhodamine B (SRB) stain-based colorimetric assay and hemocytometer counting were used to determine the reduction in A. castellanii trophozoite proliferation and encystation, in response to treatment with C. sinensis or EGCg. Fourier transform infrared (FTIR) microscopy was used to analyze chemical changes in the trophozoites and cysts due to C...
September 17, 2020: Pathogens
Milan C Samarakoon, Sajeewa S N Maharachchikumbura, Jian-Kui Jack Liu, Kevin D Hyde, Itthayakorn Promputtha, Marc Stadler
Amphisphaeriaceous taxa (fungi) are saprobes on decaying wood in terrestrial, mangrove, and freshwater habitats. The generic boundaries of the family have traditionally been based on morphology, and the delimitation of genera has always been challenging. Amphisphaeria species have clypeate ascomata and 1-septate ascospores and a coelomycetous asexual morph. Lepteutypa is different from Amphisphaeria in having eutypoid stromata and more than 1-septate ascospores. These main characters have been used for segregation of Lepteutypa from Amphisphaeria for a long time...
September 17, 2020: Journal of Fungi (Basel, Switzerland)
Xinwan Zhang, Hongling Liu, Elizabeth Pilon-Smits, Wei Huang, Pu Wang, Mingle Wang, Fei Guo, Yu Wang, Ruiyuan Li, Hua Zhao, Dejiang Ni
The vigor of tea plants ( Camellia sinensis ) and tea quality are strongly influenced by the abundance and forms of nitrogen, principally NO3 - , NH4 + , and amino acids. Mechanisms to access different nitrogen sources and the regulatory cues remain largely elusive in tea plants. A transcriptome analysis was performed to categorize differentially expressed genes (DEGs) in roots and young leaves during the early response to four nitrogen treatments. Relative to the continuously nitrogen-replete control, the three nitrogen-deprived and resupplied treatments shared 237 DEGs in the shoots and 21 DEGs in the root...
September 17, 2020: Plants (Basel, Switzerland)
Meifeng Li, Jianjun Liu, Yuping Zhou, Siqin Zhou, Shuai Zhang, Huarong Tong, Aichun Zhao
Conventional wisdom holds that tea (Camellia sinensis) quality can be improved by drought. To clarify the underlying mechanism, a conjoint analysis of transcriptome and metabolome profiling was carried out in tea shoots harvested under different soil water contents (SWCs). Drought had little impact on theanine, catechins and caffeine in field conditions. Besides the flavor contributions of amino acid and their derivatives, organic acids, and nucleotides and their derivatives, the obviously increased isoflavonoids and glycosylflavonoids and the sharply decreased lipids are suggested to play key roles, which is mainly due to substantial increases of type III polyketide synthase B (PKSB), flavonol synthase/flavanone 3-hydroxylase (FLS), and UDP-glycosyltransferases (UGTs), as well as the significant repression of anthocyanidin synthase (ANS) and R2R3MYBs, and downregulated lipid metabolisms...
September 21, 2020: Phytochemistry
Cristian Baicus, Larisa Pinte, Laura E Stoichitoiu, Camelia Badea
INTRODUCTION: There was no evidence concerning the prophylaxis with hydroxychloroquine, and only low-grade evidence regarding the use of hydroxychloroquine as a treatment for COVID-19 patients. We performed a survey among Romanian physicians in order to see how many of them would administer prophylactically hydroxychloroquine to themselves or to people close to them, and if they would participate to a randomized controlled trial. METHODS: Between March 30 and April 02, 2020, a 16-item questionnaire was shared in a Romanian Facebook group of 2645 physicians dedicated to COVID-19 information, asking to be completed by physicians who could be directly involved in the care of these patients...
September 21, 2020: Journal of Evaluation in Clinical Practice
Sayed Sartaj Sohrab, Mohd Suhail, Mohammad Amjad Kamal, Fahim Ahmad, Esam Ibraheem Azhar
BACKGROUND: Human coronaviruses (HCoV) are common viruses and known to be associated with respiratory diseases, including pneumonia. Currently, there are seven human coronaviruses have been identified and known to cause upper and lower respiratory infections as well as nosocomial viral infections in human. The bats, palm civets and camels are identified as the reservoir of human coronaviruses. In 2002-2003, the emergence of SARS-CoV resulted in an outbreak and led towards the more awareness and importance of scientific research and medical urgency...
August 21, 2020: Current Pharmaceutical Design
Camelia Qian Ying Tang, Sean Han Sheng Lai
No abstract text is available yet for this article.
September 11, 2020: Journal of hand surgery global online
Radu Dragos Marcu, Dan Liviu Dorel Mischianu, Lucian Iorga, Camelia Cristina Diaconu, Mihaela Surcel, Adriana Narcisa Munteanu, Carolina Constantin, Gheorghita Isvoranu, Ovidiu Gabriel Bratu
Pelvic organ prolapse is a frequent health problem in women, encountered worldwide, its physiopathology being still incompletely understood. The integrity of the pelvic-supportive structures is a key element that prevents the prolapse of the pelvic organs. Numerous researchers have underlined the role of connective tissue molecular changes in the pathogenesis of pelvic organ prolapse and have raised the attention upon oxidative stress as an important element involved in its appearance. The advancements made over the years in terms of molecular biology have allowed researchers to investigate how the constituent elements of the pelvic-supportive structures react in conditions of oxidative stress...
2020: Journal of Immunology Research
Shaofeng Peng, Jia Lu, Zhen Zhang, Li Ma, Caixia Liu, Yongzhong Chen
Background: Oil-tea Camellia is a very important edible oil plant widely distributed in southern China. Tea oil extracted from the oil-tea Camellia seeds is beneficial to health and is considered as a health edible oil. We attempt to identify genes related to fatty acid biosynthesis in an oil-tea Camellia seed kernel, generated a comprehensive transcriptome analysis of the seed kernel at different developmental stages, and explore optimal picking time of fruit. Material and Methods . A gas chromatography-mass spectrometer was used to detect the content of various fatty acids in samples...
2020: International Journal of Genomics
Fatemeh Kalalinia, Nafise Amiri, Niloufar Mehrvarzian, Bibi Sedigheh Fazly Bazzaz, Mehrdad Iranshahi, Azadeh Shahroodi, Sepideh Arabzadeh, Mohammadreza Abbaspour, Shapour Badiee Aaval, Jebrail Movaffagh
Objectives: Potentially preventable death from uncontrolled hemorrhage clearly indicates the importance of simple, fast and efficient ways to achieving hemostasis. The aim of this study was to develop a topical formulation of green tea extract for reducing bleeding that can be helpful in hemorrhage control. Materials and Methods: Hydroalcoholic extract of green tea was isolated from Camellia sinensis and formulated in polyvinyl alcohol (PVA) to achieve two concentrations of 2% and 4% v/v...
August 2020: Iranian Journal of Basic Medical Sciences
Mostafa Salari, R John Milne, Camelia Delcea, Lina Kattan, Liviu-Adrian Cotfas
This paper addresses the airplane passengers' seat assignment problem while practicing social distancing among passengers. We proposed a mixed integer programming model to assign passengers to seats on an airplane in a manner that will respect two types of social distancing. One type of social distancing refers to passengers being seated far enough away from each other. The metric for this type of social distancing is how many passengers are seated so close to each other as to increase the risk of infection...
October 2020: Journal of air transport management
Thamer Aljutaily, Eduardo Huarte, Sergio Martinez-Monteagudo, Jose L Gonzalez-Hernandez, Maristela Rovai, Igor N Sergeev
Targeting gut microbiota with probiotics has emerged as a promising nutritional approach for the prevention of obesity and metabolic syndrome. Cultured dairy products can be effectively employed for the delivery of probiotics to the gut as well as for the support of growth and survival of probiotic bacteria. The purpose of this study was to characterize the effects of probiotic-enriched pasteurized milk and dairy products (Greek-style yogurt and cottage cheese) of different origins (cow, goat, and camel) on taxonomic composition of the mouse gut microbiota...
July 6, 2020: Nutrition Research
Ehsan H Abu Zeid, Nabela I El Sharkawy, Gihan G Moustafa, Abeer M Anwer, Ahmed G Al Nady
The present study investigated the alleviating role of camel milk (CM) in the mitigation of fenpropathrin (FNP) type II pyrethroid induced oxidative stress, alterations of hepatic (CYP1A1) mRNA expression pattern, and DNA damage using the alkaline comet assay (SCGE) in male rats. Sixty male Sprague-Dawley rats were separated into six groups (n = 10): 1st control (C), 2nd corn oil (CO), 3rd (CM): gavaged CM 2ml/rat, 4th (FNP): gavaged FNP 7.09 mg/kg body weight (BW), 5th (FNP pro/co-treated): gavaged CM firstly for 15 days, then CM + FNP by the same mentioned doses and route, 6th (FNP + CM co-treated): gavaged FNP firstly followed by CM by the same mentioned doses and route...
September 16, 2020: Ecotoxicology and Environmental Safety
Martin Wainaina, Gabriel O Aboge, Isaac Omwenga, Catherine Ngaywa, Nicholas Ngwili, Henry Kiara, George Wamwere-Njoroge, Bernard Bett
INTRODUCTION: Brucellosis is an important zoonotic disease in Kenya, and identifying the bacteria in milk is important in assessing the risk of exposure in people. METHODS: A cross-sectional study that involved 175 households was implemented in the pastoral counties of Marsabit and Isiolo in Kenya. Pooled milk samples (n = 164) were collected at the household level, and another 372 were collected from domesticated lactating animals (312 goats, 7 sheep, 50 cattle and 3 camels)...
September 18, 2020: Tropical Animal Health and Production
Stéphanie Mali Jost, Andrea Knoll, Gesine Lühken, Cord Drögemüller, Patrik Zanolari
BACKGROUND: The increasing popularity of alpacas and llamas outside of South America is undeniable. The associated limited genetic diversity raises questions about health and other genetically determined traits like coat colour. Therefore, a survey studying the prevalence of congenital disorders and coat colours and patterns in South American camelids was performed in Austria, Germany and Switzerland. Moreover, the motivation for keeping these animals, the herd size and breeds was assessed...
September 18, 2020: Acta Veterinaria Scandinavica
Max R Paquette, Kris Camelio, Allison H Gruber, Douglas W Powell
CONTEXT: Changes in lower limb loading and movement quality after prolonged running and training periods might influence injury risks in runners. OBJECTIVES: To assess (1) the effects of a single prolonged run and a 3-week running training program on peak tibial acceleration (PTA) during running and Functional Movement Screen (FMS) criterion tests, and (2) the relationship between running volume during the 3-week training program and changes in PTA and FMS scores after training...
September 18, 2020: Journal of Athletic Training
S E Grayzel, G R Thompson, B Martínez-López, J E Dechant, I McHardy, J E Sykes
Given the predisposition of South American camelids to coccidioidomycosis, we sought to describe the disease presentation in alpacas and llamas and identify potential risk factors for these species. The records of 224 llamas and alpacas that were tested for Coccidioides infection using immunodiffusion serology at the Coccidioidomycosis Serology Laboratory of the University of California, Davis, between 1990 and 2016 were examined; of those, 46 alpacas and 42 llamas had positive test results. The remaining 99 alpacas and 37 llamas were used as control groups...
September 17, 2020: Medical Mycology
Mostafa Meshref, Yara Amro, Mahmoud Ahmed Ebada
BACKGROUND: Brucellosis is a highly infectious multi-systemic zoonosis, and it is caused by Gram-negative bacteria, Brucella. Despite the low incidence of neurobrucellosis, it is the most dangerous consequence of brucellosis. CASE REPORT: A 30-year-old Sudanese male patient presented to our hospital with a complaint of fever associated with confusion for three days. He had signs of meningeal irritation in the form of neck stiffness, positive Kernig's, and Lesage's sign...
September 17, 2020: Recent Patents on Anti-infective Drug Discovery
Ștefan Cristian Gherghina, Daniel Ștefan Armeanu, Camelia Cătălina Joldeș
This paper examines the linkages in financial markets during coronavirus disease 2019 (COVID-19) pandemic outbreak. For this purpose, daily stock market returns were used over the period of December 31, 2019-April 20, 2020 for the following economies: USA, Spain, Italy, France, Germany, UK, China, and Romania. The study applied the autoregressive distributed lag (ARDL) model to explore whether the Romanian stock market is impacted by the crisis generated by novel coronavirus. Granger causality was employed to investigate the causalities among COVID-19 and stock market returns, as well as between pandemic measures and several commodities...
September 15, 2020: International Journal of Environmental Research and Public Health
Thierry Prazuck, Mathilda Colin, Susanna Giachè, Camélia Gubavu, Aymeric Seve, Vincent Rzepecki, Marie Chevereau-Choquet, Catherine Kiani, Victor Rodot, Elsa Lionnet, Laura Courtellemont, Jérôme Guinard, Gilles Pialoux, Laurent Hocqueloux
BACKGROUND: The SARS-CoV-2 (Severe Acute Respiratory Syndrome CoronaVirus 2) is responsible for the infectious respiratory disease called COVID-19 (COronaVIrus Disease 2019). In response to the growing COVID-19 pandemic, point-of-care (POC) tests have been developed to detect specific antibodies, IgG and IgM, to SARS-CoV-2 virus in human whole blood. We conducted a prospective observational study to evaluate the performance of two POC tests, COVID-PRESTO® and COVID-DUO®, compared to the gold standard, RT-PCR (real-time reverse transcriptase polymerase chain reaction)...
2020: PloS One
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