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Nano Biosensor

Ruchita Shandilya, Arpit Bhargava, Neha Bunkar, Rajnarayan Tiwari, Irina Yu Goryacheva, Pradyumna Kumar Mishra
Early cancer diagnosis is of prime importance as it paves the way for effective treatment and possible patient survival. The recent advancements in the field of biosensorics have facilitated the development of functionalized nanobiosensors which have the potential to provide a cost-effective, reliable and rapid diagnostic strategy for cancers. These nanoscaled sensing systems utilize electrochemical, optical, mass and calorimetric sensing mechanisms to specifically identify the disease-specific biomarkers. Because of clinical translational utility, the present review aims to describe the recent developments and status of the nanobiosensors as a point-of-care approach for cancer diagnosis...
January 22, 2019: Biosensors & Bioelectronics
Massimiliano Magro, Davide Baratella, Giovanni Miotto, Jan Frömmel, Marek Šebela, Martina Kopečná, Enzo Agostinelli, Fabio Vianello
The preservation of enzymatic activity is a fundamental requirement for exploiting hybrid nano-bio-conjugates, and the control over protein-nanoparticle interactions, leading to stable and catalytically active hybrids, represents the key for designing new biosensing platforms. In this scenario, surface active maghemite nanoparticles (SAMNs) represent a new class of naked magnetic nanoparticles, displaying peculiar electrocatalytic features and the ability to selectively bind proteins. Recombinant aminoaldehyde dehydrogenase from tomato (SlAMADH1) was used as a model protein, and successfully immobilized by self-assembly on the surface of naked SAMNs, where its enzymatic activity resulted preserved for more than 6 months...
February 6, 2019: Amino Acids
Razieh Salahandish, Ali Ghaffarinejad, Seyed Morteza Naghib, Asghar Niyazi, Keivan Majidzadeh-A, Mohsen Janmaleki, Amir Sanati-Nezhad
We present a highly sensitive and selective nano-biosensor for rapid, stable and highly reproducible detection of ascorbic acid (AA) in the presence of dopamine, uric acid and other interferences by a three-layer sandwich arrangement of nitrogen-doped functionalized graphene (NFG), silver nanoparticles (AgNPs) and nanostructured polyaniline (PANI) nanocomposite. The enhanced AA electrochemical properties of the NFG/AgNPs/PANI electrode is attributed to the superior conductivity of the NFG-PANI and the excellent catalytic activity of AgNPs...
February 4, 2019: Scientific Reports
Chih-Yuan Lin, Cody Combs, Yen-Shao Su, Li-Hsien Yeh, Zuzanna S Siwy
Nanopores exhibit a set of interesting transport properties that stem from interactions of the passing ions and molecules with the pore walls. Nanopores are used e.g. as ionic diodes and transistors, biosensors, and osmotic power generators. Using nanopores is however disadvantaged by their high resistance, small switching currents in nA range, low power generated, and signals that can be difficult to distinguish from the background. Here, we present a mesopore with ionic conductance reaching S that rectifies ion current in salt concentrations as high as 1 M...
February 3, 2019: Journal of the American Chemical Society
Rozita Abolhasan, Amir Mehdizadeh, Mohammad Reza Rashidi, Leili Aghebati-Maleki, Mehdi Yousefi
Biosensors have been commonly used in biomedical diagnostic tools in recent years, because of a wide range of application, such as point-of-care monitoring of treatment and disease progression, drug discovery, commonly use food control, environmental monitoring and biomedical research. Additionally, development of DNA biosensors has been increased enormously over the past few years as confirmed by the large number of scientific publications in this field. A wide range of techniques can be used for the development of DNA biosensors, such as DNA nano-machines and various signal amplification strategies...
January 15, 2019: Biosensors & Bioelectronics
Rui Zhang, Xia Zhong, An-Yi Chen, Jia-Li Liu, Sheng-Kai Li, Yaqin Chai, Ying Zhuo, Ruo Yuan
A sensitive electrochemiluminescence (ECL) biosensor was developed for glutathione (GSH) detection based on a novel Ru(bpy)2(cpaphen)2+/TPrA/TiO2 ternary ECL system with Mn2+ as substitute target for signal amplification. Specifically, the TiO2 nano-needles (TiO2 NNs) were used as the co-reaction accelerator for the first time to promote the oxidation process of co-reactant tripropylamine (TPrA) in the anode and significantly increase the ECL signal of Ru(bpy)2(cpaphen)2+ for an amplified initial signal. Meanwhile, a novel target conversion strategy for GSH was developed by reducing MnO2 nanosheets to Mn2+ as a substitute target, which played the role of a co-enzyme factor for cleaving DNA double strands intercalated with Ru(bpy)2(cpaphen)2+ to markedly weaken initial signal...
January 30, 2019: Analytical Chemistry
Aihua Jing, Gaofeng Liang, Yixin Yuan, Wenpo Feng
The quantification of ascorbic acid (AA), dopamine (DA), and uric acid (UA) has been an important area of research, as these molecules' determination directly corresponds to the diagnosis and control of diseases of nerve and brain physiology. In our research, graphene oxide (GO) with nano pores deposited with gold nanoparticles were self-assembled to form three-dimensional (3D) Au/holey-graphene oxide (Au/HGO) composite structures. The as-prepared 3DAu/HGO composite structures were characterized for their structures by X-ray diffraction, Raman spectrum, scanning electron microscopy, and transmission electron microscopy coupled with cyclic voltammograms...
January 24, 2019: Micromachines
Lingying Xia, Yingning Zheng, Wenbin Liang, Mengjie Li, Tao Hu, Ruo Yuan, Yaqin Chai
Here, a novel co-sensitization photoelectrochemical (PEC) strategy was established using a donor-acceptor-type photoactive material poly{4,8-bis[5-(2-ethylhexyl)thiophen-2-yl]benzo[1,2-b:4,5-b']dithiophene-2,6-diyl-alt-3-fluoro-2-[(2-ethylhexyl)carbonyl] Thieno[3,4-b]thiophene-4,6-diyl} (PTB7-Th) as signal indicator, which was co-sensitized by bis(4,4'dicarboxyl-2,2'-bipyridyl)(4,5,9,14-tetraaza-benzo[b]triphenylene)ruthenium(II) ([Ru(dcbpy)2dppz]2+) embedded in the grooves of DNA duplex and fullerene (nano-C60) immobilized on the surface of DNA nanoflowers (NFs) for microRNA assay...
January 24, 2019: Chemistry: a European Journal
Amirhossein Ghaemi, Kaveh Abdi, Shohreh Javadi, Masoud Zare Shehneh, Fatemeh Yazdian, Meisam Omidi, Hamid Rashedi, Bibi Fatemeh Haghiralsadat, Omid Asayeshnaeini
Sulfur compounds are essential for many industries and organisms; however, they cause serious respiratory problems for human beings. Therefore, determination of sulfur concentration is of paramount importance. The research approach in the field of detecting contaminants has led to smaller systems that provide faster and more effective ways for diagnosis purposes. In this study, a novel portable amperometric graphene oxide-protein biosensor platform is investigated. The main characteristic of this structure is the implementation of a micro-fluidic configuration...
January 22, 2019: Biotechnology and Applied Biochemistry
Bharath Babu Nunna, Debdyuti Mandal, Joo Un Lee, Harsimranjit Singh, Shiqiang Zhuang, Durgamadhab Misra, Md Nasir Uddin Bhuyian, Eon Soo Lee
Integrating microfluidics with biosensors is of great research interest with the increasing trend of lab-on-the chip and point-of-care devices. Though there have been numerous studies performed relating microfluidics to the biosensing mechanisms, the study of the sensitivity variation due to microfluidic flow is very much limited. In this paper, the sensitivity of interdigitated electrodes was evaluated at the static drop condition and the microfluidic flow condition. In addition, this study demonstrates the use of gold nanoparticles to enhance the sensor signal response and provides experimental results of the capacitance difference during cancer antigen-125 (CA-125) antigen-antibody conjugation at multiple concentrations of CA-125 antigens...
January 17, 2019: Nano Convergence
Majid Arvand, Aazam A Mirroshandel
In this paper a new aptasensor with high-sensitivity, and high-specificity for detection of diazinon, was discovered, based on fluorescence resonance energy transfer (FRET) between quantum dot (QD) as a donor and graphene oxide (GO) as an acceptor. l-cysteine capped CdS QDs/DF20 aptamer bioconjugates were successfully synthesized. GO was also attached to aptamers and photoluminescence quenching was obtained through FRET. By adding target diazinon to the bioconjugates containing GO, photoluminescence recovery was detected due to detachment of GO from the aptamer as a result of the difference in affinity for the aptamer...
May 15, 2019: Food Chemistry
Fanmao Liu, Zhihong Lin, Quanchang Jin, Qianni Wu, Chengduan Yang, Hui-Jiuan Chen, Zihan Cao, Di-An Lin, Lingfei Zhou, Tian Hang, Gen He, Yonghang Xu, Wenhao Xia, Jun Tao, Xi Xie
Real-time transdermal biosensing provides a direct route to quantify biomarkers or physiological signals of local tissues. Although microneedles (MNs) present a mini-invasive transdermal technique, integration of MNs with advanced nanostructures to enhance sensing functionalities has rarely been achieved. This is largely due to the fact that nanostructures present on MNs surface could be easily destructed due to friction during skin insertion. In this work, we reported a dissolvable polymer-coating technique to protect nanostructures-integrated MNs from mechanical destruction during MNs insertion...
February 6, 2019: ACS Applied Materials & Interfaces
Ying Yu, Chunguang Li, Cailing Chen, He Huang, Chen Liang, Yue Lou, Xiao-Bo Chen, Zhan Shi, Shouhua Feng
Photoluminescence(PL) nano-biosensors that can be used for accurately and reliably monitoring pH and vitamin hold a great promise in biology and medicine. Herein, a high quantum yield of 16% saccharomyces-derived N-doped carbon dots (s-N-CDs) was synthesized through a simple and one-pot microwave-assisted hydrothermal approach. The produced s-N-CDs are an excellent multi-functional biosensor for the applications of pH sensing and vitamin probing. Fluorescence intensity and fluorescence lifetime dramatically increases with pH decreasing from 14 to 2...
April 1, 2019: Talanta
Aryane Tofanello, Juscemácia N Araujo, Iseli L Nantes-Cardoso, Fabio F Ferreira, José A Souza, Dae-Woon Lim, Hiroshi Kitagawa, Wanius Garcia
Solid-state ionic conductor is an essential and critical part of electrochemical devices such as batteries and sensors. Nano-sized silver iodide (AgI) is the most promising ionic conductor due to its superionic conductivity at room temperature. In recent years, proteins have been used as organic templates to obtain high-performance solid-state ionic conductors as well as to extend their applications in a biosensor. Here, we report the unprecedented ultrafast synthesis of thermally stable protein-coated AgI nanoparticles (NPs) through the photo-irradiation method for solid-state electrolyte...
December 21, 2018: Colloids and Surfaces. B, Biointerfaces
Radha D Singh, Ruchita Shandilya, Arpit Bhargava, Rajat Kumar, Rajnarayan Tiwari, Koel Chaudhury, Rupesh K Srivastava, Irina Y Goryacheva, Pradyumna K Mishra
Lung cancer is the most frequently occurring malignancy and the leading cause of cancer-related death for men in our country. The only recommended screening method is clinic based low-dose computed tomography (also called a low-dose CT scan, or LDCT). However, the effect of LDCT on overall mortality observed in lung cancer patients is not statistically significant. Over-diagnosis, excessive cost, risks associated with radiation exposure, false positive results and delay in the commencement of the treatment procedure questions the use of LDCT as a reliable technique for population-based screening...
2018: Frontiers in Genetics
Chiara Cozzi, Giovanni Polito, Lucanos M Strambini, Giuseppe Barillaro
The anodic dissolution of silicon in acidic electrolytes is a well-known technology enabling the silicon machining to be accurately controlled down to the micrometer scale in low-doped n -type silicon electrodes. Attempts to scale down this technology to the submicrometer scale has shown to be challenging, though it premises to enable the fabrication of meso and nano structures/systems that would greatly impact the fields of biosensors and nanomedicine. In this work, we report on the electrochemical etching at high anodic voltages (up to 40 V) of two-dimensional regular arrays of millions pores per square centimeter (up to 30 × 106 cm-2 ) with sub-micrometric diameter (down to ~860 nm), high depth (up to ~40 μm), and high aspect-ratio (up to ~45) using low-doped n -type silicon electrodes (resistivity 3-8 Ω cm)...
2018: Frontiers in Chemistry
Shuo Wang, Zhaofu Zhang, Linfeng Dong, Geoffrey I N Waterhouse, Qihai Zhang, Lifang Li
Herein, we report the successful synthesis of a series of poly (N-isopropylacrylamide) (PNIPA)/layered double hydroxides (LDHs)/nano-hydroxyapatite (nano-HA) hydrogels via in-situ radical polymerization. The internal morphology, thermo sensitivity, rheological properties, swelling behavior and hemocompatibility of the PNIPA/LDHs/HA composite hydrogels were systematically investigated. Results show that the hydrogels had a reversible sol-gel transformation around 33 °C. Interactions between the positively charged LDHs and negatively charged nano-HA particles created a highly porous hydrogel network...
December 4, 2018: Journal of Colloid and Interface Science
Sang-Hyun Oh, Hatice Altug
Nanoplasmonic structures can tightly confine light onto a material's surface to probe biomolecular interactions not easily accessed by other sensing techniques. New and exciting developments in nanofabrication processes, nano-optical trapping, graphene devices, mid-infrared spectroscopy, and metasurfaces will greatly empower the performance and functionalities of nanoplasmonic sensors.
December 10, 2018: Nature Communications
Stefano Mariani, Valentina Robbiano, Lucanos M Strambini, Aline Debrassi, Gabriela Egri, Lars Dähne, Giuseppe Barillaro
Nanostructured materials premise to revolutionize the label-free biosensing of analytes for clinical applications, leveraging the deeper interaction between materials and analytes with comparable size. However, when the characteristic dimension of the materials reduces to the nanoscale, the surface functionalization for the binding of bioreceptors becomes a complex issue that can affect the performance of label-free biosensors. Here we report on an effective and robust route for surface biofunctionalization of nanostructured materials based on the layer-by-layer (LbL) electrostatic nano-assembly of oppositely-charged polyelectrolytes, which are engineered with bioreceptors to enable label-free detection of target analytes...
December 10, 2018: Nature Communications
Kai U Cormann, Meike Baumgart, Michael Bott
The development of biosensors for in vitro quantification of small molecules such as metabolites or man-made chemicals is still a major challenge. Here we show that engineered variants of the sensory PAS domain of the histidine kinase CitA of the thermophilic bacterium Geobacillus thermoleovorans represent promising alternatives to established biorecognition elements. By combining binding site grafting and rational design we constructed protein variants binding l-malate, ethylmalonate, or the aromatic compound phthalate instead of the native ligand citrate...
December 10, 2018: ACS Synthetic Biology
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