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ovarian granulosa cell

Dejun Xu, Huanshan He, Xiaohan Jiang, Lulu Yang, Dinbang Liu, Li Yang, Guoxia Geng, Jianyong Cheng, Huali Chen, Rongmao Hua, Jiaxin Duan, Xiaoya Li, Lin Wu, Yuan Li, Qingwang Li
Steroid hormones are required for normal reproductive function of female. The aim of this study was to investigate the role of Raf-ERK1/2 on steroid hormone synthesis in bovine ovarian granulosa cells. Immunohistochemistry assay showed that both B-Raf and C-Raf were expressed in granulosa cells, theca cells and sertoli cells. The protein expression of Raf or ERK1/2 was clearly decreased by Raf inhibitor GSK2118436 or ERK1/2 inhibitor SCH772984, respectively (P<0.05). In addition, western blotting was performed for investigating the cross-talk between Raf and ERK1/2, the data showed that Raf positively regulated ERK1/2, whereas, ERK1/2 played a negative feedback effect on Raf...
February 20, 2019: Reproduction in Domestic Animals, Zuchthygiene
Sandro Sacchi, Elena Tenedini, Debora Tondelli, Sandra Parenti, Daniela Tagliasacchi, Susanna Xella, Tiziana Marsella, Enrico Tagliafico, Antonio La Marca
Using recombinant DNA technologies, a chimeric gene containing the coding sequences of follicle stimulating hormone (FSH) β-subunit and C-terminal peptide of the human chorionic gonadotrophin (hCG) β-subunit have been designed to generate a new gonadotrophin named corifollitropin alfa (CFA). CFA has longer elimination half-life and slower rate of absorption compared with FSH, which makes CFA a long-acting hormone employed as a substitute of the recombinant FSH (recFSH) in the controlled ovarian stimulation (COS)...
February 20, 2019: Gynecological Endocrinology
Jinghe Cao, Gulijianati Maowulieti, Tao Yu
Expression of peroxisome proliferator-activated receptor γ (PPARγ) mRNA in ovarian granulosa cells of patients with polycystic ovary syndrome (PCOS) were explored. Ovarian granulosa cells were extracted from 5 patients with PCOS and 30 normal controls. Expression of PPARγ mRNA in granulosa cells of the 5 PCOS patients (observation group) and 5 normal controls (control group) was detected by RT-qPCR. The remaining 25 cases of normal human ovarian granulosa cells were cultured in vitro for 48 h, followed by cell culture for another 24 h with different concentrations of testosterone, insulin (INS), and rosiglitazone (RGZ)...
March 2019: Experimental and Therapeutic Medicine
Ping Yuan, Ruiqi Li, Di Li, Lingyan Zheng, Songbang Ou, Haijing Zhao, Qingxue Zhang, Wenjun Wang
PURPOSE: Empty follicle syndrome (EFS) is a complex reproductive disorder characterized by the repeated failure to aspirate oocytes from mature ovarian follicles during in vitro fertilization (IVF). In addition to some cases caused by iatrogenic problems and known genetic factors, there are still many unexplained aspects of EFS. Here, we aimed to assess the clinical and genetic characteristics of two EFS patients. METHODS: We have characterized two primary infertility patients with EFS in a nonconsanguineous family from China...
February 18, 2019: Journal of Assisted Reproduction and Genetics
Namya Mellouk, Christelle Ramé, Mélodie Diot, Eric Briant, Jean-Luc Touzé, Daniel Guillaume, Pascal Froment, Joëlle Dupont
BACKGROUND: In dairy cows, the energy cost of milk yield results in a negative energy balance (EB) and body fat mobilization that impairs reproductive efficiency. Emerging evidence suggests that the novel adipokines, Retinoic acid receptor responder protein 2 (RARRES2), and its main receptor, Chemokine-like receptor 1 (CMKLR1) are involved in the regulation of metabolic and ovarian functions. So, we investigated in a first experiment the plasma RARRES2, and RARRES2 and CMKLR1 mRNA expression levels in subcutaneous adipose tissue (SAT) and granulosa cells (GC) at different times of body fat mobilization in dairy cows (4, 8, 20 and 44 weeks postpartum, wk...
February 18, 2019: Reproductive Biology and Endocrinology: RB&E
Mengshi Fang, Jiangning Dong, Qun Zhong, Xin Fang, Yulan Chen, Chuanbin Wang, Hong Yan
BACKGROUND: Magnetic resonance imaging (MRI) is a significant method of identifying ovarian tumors, when combined with certain functional imaging techniques that have been considered to be more accurate. PURPOSE: Our study aims to determine the value of diffusion-weighted imaging (DWI) combined with MRI in the diagnosis of thecomas and adult ovarian granulosa cell tumors (GCTs). MATERIAL AND METHODS: In total, this study included 10 patients with thecomas and nine with adult GCTs...
February 18, 2019: Acta Radiologica
Martha E Stewart, Anne T Knisely, Mackenzie W Sullivan, Kari L Ring, Susan C Modesitt
The objectives of this study were to determine both surgical and subsequent cancer outcomes for high-risk women from the University of Virginia's High-Risk Breast/Ovarian Cancer clinic undergoing ovarian cancer risk-reducing surgery. Retrospective review identified high risk women who had ovarian risk reducing surgery over the past decade and surgical outcomes, pathology, pre-operative screening results, and pre-/post-operative cancer diagnoses were evaluated. One hundred and eighty-three high-risk women had risk reducing surgery at a mean age of 50...
May 2019: Gynecologic Oncology Reports
D Lim, E Oliva
Approximately 5% of gynecological malignancies are associated with paraneoplastic hypercalcemia. Awareness of its association with certain tumor types allows for earlier disease detection and facilitates monitoring of treatment response and disease recurrence. We review the salient clinicopathological features, differential diagnosis and management issues in some of these gynecological tumors, namely: small cell carcinoma, hypercalcemic type; ovarian clear cell carcinoma, dysgerminoma and juvenile granulosa cell tumors...
February 1, 2019: Seminars in Diagnostic Pathology
Robert O Gilbert
Diseases of postpartum dairy cows impair reproductive processes, resulting in prolonged anestrus, reduced conception, and increased pregnancy attrition, regardless of whether the initial disease precedes insemination (even by many weeks), occurs close to insemination, or follows fertilization. Bacteria and their products activate pattern recognition receptors that respond to pathogen-associated molecular patterns (PAMP). These receptors include toll-like receptors (TLR), nucleotide-binding oligomerization domain (NOD)-like receptors and others, and their activation culminates in upregulation of proinflammatory cytokines such as IL-1β, IL-18, and tumor necrosis factor-α...
February 13, 2019: Journal of Dairy Science
Meijiao Wang, Dan Zhao, Liangzhi Xu, Wenjing Guo, Li Nie, Yi Lei, Yun Long, Min Liu, Yichen Wang, Xueqin Zhang, Li Zhang, Hanna Li, Jinhu Zhang, Dongzhi Yuan, Limin Yue
BACKGROUND: Proprotein convertase subtilisin / kexin type 9 (PCSK9) plays a critical role in the cholesterol metabolism by negatively regulating the low-density lipoprotein receptor (LDLR). Lipid metabolic and ovarian disorders are the common clinical manifestation of polycystic ovary syndrome (PCOS). Here, we intended to elucidate the role of PCSK9 in the pathogenesis of PCOS conducted on a human population in case-control design and animal part in an interventional study. METHODS: We firstly investigated the serum levels of PCSK9 in 46 PCOS patients compared with 49 healthy women as controls, and then developed a PCOS mouse model induced by dehydroepiandrosterone (DHEA) and a high-fat diet (HFD) to determine the role of PCSK9 in abnormal lipid metabolism and ovarian dysfunction of PCOS in four groups (n = 40 per group): control, PCOS mice, PCOS plus alirocumab group, and PCOS plus vehicle group...
February 12, 2019: Metabolism: Clinical and Experimental
Leila Mirzaeian, Poopak Eftekhari-Yazdi, Fereshteh Esfandiari, Farideh Eivazkhani, Mojtaba Rezazadeh Valojerdi, Ashraf Moini, Rouhollah Fathi
The peritoneum mesothelium lines body cavities and has the same origin as ovarian surface epithelium with probable existence of peritoneum mesenchymal stem cells (PMSCs). In the present research, PMSCs were isolated from peritoneum and differentiated into ovarian cell like cells using human follicular fluid (HFF) and human cumulus conditioned medium (HCCM). Anterior abdominal wall and intestinal peritoneum explants were used for cells isolation and cultured in DMEM. After passage 3, purified PMSCs were assessed for morphology, proliferation rate and cell viability...
February 15, 2019: Stem Cells and Development
Daniela Figueroa, Mohammad Asaduzzaman, Fiona Young
The combination of doxorubicin and cyclophosphamide commonly used to treat breast cancer can cause premature ovarian failure and infertility. α -Tocopherol is a potent antioxidant whereas γ -tocopherol causes apoptosis in a variety of cancer models in vitro including breast cancer. We hypothesised that the combination of doxorubicin (Dox) and 4-hydroperoxycyclophosphamide (4-Cyc) would be more cytotoxic in vitro than each agent alone, and that α -tocopherol would reduce and γ -tocopherol would augment the cytotoxicity of the combined chemotherapeutics...
2019: BioMed Research International
Priyadarshini Singh Nee Priyadarshini, Bechan Lal
Authors have recently reported a gradual increase in neuropeptide Y expression in the ovarian follicles of Clarias batrachus with the progression of oogenesis, coinciding with increasing photoperiod and temperature. This indicates the involvement of photoperiod and temperature in controlling NPY expression. Therefore, a study was designed to investigate the role of photoperiod and temperature in regulation of NPY expression in ovarian follicles. The catfish were exposed to different photo-thermal regimes during the late-quiescence and late-recrudescence phases for one month, and the expression of NPY was analyzed along with other ovarian activities...
February 11, 2019: General and Comparative Endocrinology
M Brązert, D Iżycki, W Kranc, B Borowiec, M Popis, P Celichowski, K Ożegowska, M Jankowski, M Jeseta, L Pawelczyk, A Bręborowicz, D Rachoń, M Nowicki, B Kempisty
The ovarian granulosa cells (GCs) that form the structure of follicle undergo substantial modification during the various stages of human folliculogenesis. These modifications include morphological changes, accompanied by differential expression of genes, encoding proteins which are mainly involved in cell growth, proliferation and differentiation. Recent data bring a new insight into the aspects of GCs' stem-like specificity and plasticity, enabling their prolonged proliferation and differentiation into other cell types...
February 14, 2019: Journal of Biological Regulators and Homeostatic Agents
Xiaoping Xin, Zhonghui Li, Yuyi Zhong, Qingqing Li, Jiaying Wang, Hao Zhang, Xiaolong Yuan, Jiaqi Li, Zhe Zhang
Previous studies have strongly recommended that KISS-1 metastasis suppressor ( KISS1 ) plays an essential gatekeeper of the initiation of reproductive maturation in mammals. However, KISS1 has been recently reported to highly express in ovarian granulosa cells (GCs). But the biological functionalities of KISS1 on cell apoptosis, cell cycle, and synthesis of estradiol-17β (E2) have not been explored in GCs. In this study, using porcine GCs as a cellular model, the overexpression plasmid of KISS1 was built to explore the biological effects of KISS1 on the PI3K signaling pathway, estrogen signaling pathway, cell apoptosis, cell cycle, and E2 secretion...
February 12, 2019: Animals: An Open Access Journal From MDPI
Prapti Singh, Ariela Agress, Vanessa Madrigal, Clara Magyar, Nora Ostrzega, Gregorio Daniel Chazenbalk, Daniel Anthony Dumesic
Context: Ovarian hyperandrogenism from polycystic ovarian syndrome (PCOS) and hyperinsulinemia from insulin resistance are modulators of ovarian follicle development. We report a PCOS woman with hyperandrogenism and severe insulin resistance from metabolic syndrome who received long-term GnRH analog therapy preceding bilateral salpingo-oophorectomy for massive ovarian enlargement. Ovarian histological examination showed proliferating granulosa cells within antral follicles coexistent with serous cystadenofibromas, demonstrating a unique link between hyperinsulinemia and granulosa cell mitogenesis...
February 12, 2019: Journal of Clinical Endocrinology and Metabolism
Hu Tao, Qi Xiong, Ziyun Ji, Feng Zhang, Yang Liu, Mingxin Chen
MicroRNAs (miRNAs) play key roles in mammalian folliculogenesis (a complex process in which primordial follicles develop into mature oocytes) by inhibiting mRNA translation or by inducing its degradation, while the role of miRNA in folliculogenesis and regulation mechanism remain unclear. In this study, we explored the role of the p53/miR-27a/nuclear factor of activated T-cells 5 (NFAT5) signaling axis in mouse ovarian granulosa cell proliferation. Luciferase reporter assay, overexpression, site-directed mutagenesis, and chromatin immunoprecipitation (ChIP) assay results showed that the transcription factor p53 significantly decreased the expression level of miR-27a by binding to sites 4 (-646 to -637 bp) and 10 (-50 to -41 bp) of the miR-27a promoter...
2019: International Journal of Biological Sciences
Dimitrios Nasioudis, Theofano Orfanelli, Melissa K Frey, Eloise Chapman-Davis, Thomas A Caputo, Steven S Witkin, Kevin Holcomb
OBJECTIVE: To investigate the role of adjuvant chemotherapy (CT) in the management of ovarian non-granulosa cell (GC) sex cord-stromal tumors (SCSTs). METHODS: The National Cancer Database was accessed and patients diagnosed between 2004 and 2013 with a malignant non-GC SCST were selected. Overall survival (OS) was evaluated with Kaplan-Meier curves and compared with the log-rank test. Multivariate survival analysis was performed with Cox regression. Factors associated with the administration of CT were evaluated with the chi-square test and binary logistic regression...
November 12, 2018: Journal of Gynecologic Oncology
Andrea Poch, Felipe Villanelo, Soledad Henriquez, Paulina Kohen, Alex Muñoz, Jerome F Strauss, Luigi Devoto
An estradiol metabolite, 2-methoxyestradiol (2ME), has emerged as an important regulator of ovarian physiology. 2ME is recognized as a potent anti-angiogenic agent in clinical trials and laboratory studies. However, little is known about its molecular actions and its endogenous targets. 2ME is produced by human ovarian cells during the normal menstrual cycle, being higher during regression of the corpus luteum, and is postulated to be involved in the anti-angiogenic process that plays out during luteolysis...
February 6, 2019: Steroids
Junning Yao, Zhan Li, Yeqing Fu, Ruxing Wu, Yixuan Wang, Chang Liu, Le Yang, Hanwang Zhang
In this study, we evaluated the expression and function of chemerin and CMKLR1 in the ovaries and granulosa cells of high-fat diet-induced obese (OB) mice. In vivo, chemerin/CMKLR1 system was upregulated in the serum, ovaries, and granulosa cells of OB mice compared with those in control mice. Apoptotic ovarian follicles, oxidative stress, and apoptosis biomarkers were also increased in the ovaries of OB mice. In vitro, mouse granulosa cells (mGCs) were cultured and treated with different concentrations of chemerin to investigate the effects of chemerin on viability, reactive oxygen species (ROS), and apoptosis and on the phosphorylation of AKT, AMP-activated protein kinase α (AMPKα), and nuclear factor-κB p65...
February 2, 2019: Biochemical and Biophysical Research Communications
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