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microvascular density

L Hu, X P Shu, Y Y Xu, J Cheng, Z Q Xu, J H Wang, F Lyu
Objective: To explore the changes of microcirculation of the bulbar conjunctiva after wearing the contact lens and the relationship between contact lens discomfort and bulbar conjunctiva microcirculation. Methods: Prospective cohort study. The postgraduate students from School of Ophthalmology & Optometry and School of Biomedical Engineering of Wenzhou Medical University were included in this prospective cohort study. The subjects wore soft contact lenses, and the right eye was selected as the experimental eye...
February 11, 2019: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Roberto Tamma, Tiziana Annese, Simona Ruggieri, Oronzo Brunetti, Vito Longo, Eliano Cascardi, Mauro Giuseppe Mastropasqua, Eugenio Maiorano, Nicola Silvestris, Domenico Ribatti
BACKGROUND: Cholangiocarcinoma (CCA) is the second most common subtype of primary hepatobiliary cancer and one of the most aggressive characterized by an extremely poor prognosis with limited treatment options. Inflammatory cells in tumor microenvironment support tumor growth in term of progression, angiogenesis and metastatic capacity. A link between inflammation and biliary carcinogenesis has been previously observed but the mechanisms involved remain to be determined. METHODS: We investigated the microvascular density (MVD) and inflammatory cells in tissue samples from 40 patients with CCA with locally advanced CCA and metastatic CCA by means of immunohistochemical analysis of macrophages, mast cells, B and T lymphocytes and we correlated inflammatory infiltrate with MVD...
February 14, 2019: European Journal of Clinical Investigation
Zhiping Liu, Hua Wang, Hong Jiang, Giovana Rosa Gameiro, Jianhua Wang
Background: The goal was to quantitatively analyze the bulbar conjunctival microvascular density using optical coherence tomography angiography (OCTA) and compare it to the vessel density using functional slit-lamp biomicroscopy (FSLB). Methods: Temporal bulbar conjunctiva of 20 eyes (10 healthy subjects) was imaged using both OCTA and FSLB. Image processing was performed including equalization, de-noising, thresholding, and skeletonization. The vessel density was measured by fractal analysis (box counting, Dbox) and pixel counting (%)...
2019: Eye and Vision (London, England)
Christa F Gaskill, Jonathan A Kropski, Bradley W Richmond, Robert F Foronjy, M Mark Taketo, Susan M Majka
RATIONALE: Chronic obstructive pulmonary disease (COPD) is the third leading cause of mortality in the United States. COPD and associated emphysematous loss of tissue structure often is exacerbated by vasculopathy, which substantially worsens prognosis and limits survival. Vasculopathy is characterized by remodeling and loss of microvessels. Evidence has also highlighted a role for the alterations to the microvasculature during the early pathogenesis and heterogeneity of disease, although the underlying mechanisms are not defined...
December 2018: Annals of the American Thoracic Society
Olivia Kérourédan, Jean-Michel Bourget, Murielle Rémy, Sylvie Crauste-Manciet, Jérôme Kalisky, Sylvain Catros, Noëlie B Thébaud, Raphaël Devillard
Development of a microvasculature into tissue-engineered bone substitutes represents a current challenge. Seeding of endothelial cells in an appropriate environment can give rise to a capillary-like network to enhance prevascularization of bone substitutes. Advances in biofabrication techniques, such as bioprinting, could allow to precisely define a pattern of endothelial cells onto a biomaterial suitable for in vivo applications. The aim of this study was to produce a microvascular network following a defined pattern and preserve it while preparing the surface to print another layer of endothelial cells...
February 12, 2019: Journal of Materials Science. Materials in Medicine
Roberto Tamma, Monica Rutigliano, Giuseppe Lucarelli, Tiziana Annese, Simona Ruggieri, Eliano Cascardi, Anna Napoli, Michele Battaglia, Domenico Ribatti
BACKGROUND: Clear cell renal cell carcinoma (ccRCC) represents a highly vascularized aggressive kidney cancer. Due to ccRCC chemotherapy resistance, antiangiogenesis is one of the most innovative targeted therapies for this tumor. The tumor microenvironment exerts important roles in tumor growth, angiogenesis, and metastatic escape. MATERIALS AND METHODS: In this study, we investigated the composition of tumor cell microenvironment including mast cells, macrophages, and microvascular density in ccRCC tumor tissues collected from patients who underwent nephrectomy treated or not with bevacizumab as neoadjuvant therapy before surgery...
February 6, 2019: Urologic Oncology
Roberto André Torres de Vasconcelos, Pedro Guimarães Coscarelli, Thyago Marsicano Vieira, Washington Silva Noguera, Davy Carlos Mendes Rapozo, Marcus André Acioly
Malignant peripheral nerve sheath tumors (MPNSTs) are rare and aggressive soft tissue sarcomas with a significant susceptibility to metastasize early in their course. Pathogenesis is yet to be fully elucidated. Recently, the essential role of mast cells in the tumor onset of neurofibromatosis type 1 (NF1)-associated neurofibromas and MPNSTs was confirmed in both experimental and human studies. In this study, we investigate mast cell density (MCD), microvascular density (MVD), and proliferation index (Ki-67) in MPNST...
February 8, 2019: Cancer Medicine
Yoshiki Hase, Ren Ding, Gina Harrison, Emily Hawthorne, Amilia King, Sean Gettings, Charlotte Platten, William Stevenson, Lucinda J L Craggs, Raj N Kalaria
Previous studies suggest white matter (WM) integrity is vulnerable to chronic hypoperfusion during brain ageing. We assessed ~ 0.7 million capillary profiles in the frontal lobe WM across several dementias comprising Alzheimer's disease, dementia with Lewy bodies, Parkinson's disease with dementia, vascular dementia, mixed dementias, post-stroke dementia as well as post-stroke no dementia and similar age ageing and young controls without significant brain pathology. Standard histopathological methods were used to determine microvascular pathology and capillary width and densities in 153 subjects using markers of the basement membrane (collagen IV; COL4) and endothelium (glucose transporter-1; GLUT-1)...
February 7, 2019: Acta Neuropathologica Communications
Andreas Stadlbauer, Stefan Oberndorfer, Max Zimmermann, Bertold Renner, Michael Buchfelder, Gertraud Heinz, Arnd Doerfler, Andrea Kleindienst, Karl Roessler
Treating recurrent glioblastoma (GB) is one of the challenges in modern neurooncology. Hypoxia, neovascularization, and energy metabolism are of crucial importance for therapy failure and recurrence. Twenty-one patients with initially untreated GB who developed recurrence were examined with a novel MRI approach for noninvasive visualization of the tumor microenvironment (TME). Imaging biomarker information about oxygen metabolism (mitochondrial oxygen tension) and neovascularization (microvascular density and type) were fused for classification of five different TME compartments: necrosis, hypoxia with/without neovascularization, oxidative phosphorylation, and glycolysis...
February 7, 2019: Journal of Cerebral Blood Flow and Metabolism
Eliana Costanzo, Mariacristina Parravano, Marta Gilardi, Michele Cavalleri, Riccardo Sacconi, Emanuela Aragona, Monica Varano, Francesco Bandello, Giuseppe Querques
PURPOSE: To investigate retinal and choroidal microvascular changes and structural choroidal involvement in retinal vein occlusion (RVO). METHODS: Retrospective analysis of treatment-naïve macular edema secondary to RVO, studied by optical coherence tomography (OCT) and OCT angiography (OCTA), before and after the loading phase of intravitreal injections of ranibizumab (IVR-LP). OCTA was performed using two different devices: AngioVue RTVue XR Avanti (spectral-domain OCTA) and Zeiss PLEX® Elite 9000 (swept-source OCTA)...
February 5, 2019: Ophthalmologica. Journal International D'ophtalmologie
Sasan Moghimi, Mona SafiZadeh, Masoud Aghsaei Fard, Nazgol Motamed-Gorji, Nassim Khatibi, Rebecca Chen, Robert N Weinreb
Purpose: To evaluate the changes in circumpapillary vessel density (cpVD) and retinal nerve fiber layer (RNFL) thickness after an acute primary angle closure (APAC) episode. Methods: Twenty-eight patients (28 pair of eyes) with unilateral APAC and 39 normal subjects (64 eyes) were included in this prospective, observational study. cpVD as measured by optical coherence tomography angiography and RNFL thickness as measured by spectral-domain optical coherence tomography were compared at 6 weeks after an APAC episode between affected, unaffected, and normal eyes...
February 1, 2019: Investigative Ophthalmology & Visual Science
K Sugimoto, H Murakami, T Deguchi, A Arimura, M Daimon, S Suzuki, T Shimbo, S Yagihashi
AIM: To examine the three-dimensional morphology and vascular endothelial growth factor (VEGF) expression of skin microvasculature in patients with type 2 diabetes in relation to neuropathy, retinopathy and nephropathy. METHODS: This study enrolled 17 individuals with type 2 diabetes and 16 without. Skin sections were double-immunostained for type IV collagen and VEGF-A or protein gene product (PGP)9.5. Projected images from confocal microscopy served for quantification of the occupancy rate of subepidermal type IV collagen-immunoreactive microvascular basement membrane area (OR-T4MBM), subepidermal VEGF-A-immunoreactive area, and the VEGF/T4MBM ratio as well as the PGP9...
February 4, 2019: Journal of Diabetes Investigation
Meredith A Redd, Nicole Zeinstra, Wan Qin, Wei Wei, Amy Martinson, Yuliang Wang, Ruikang K Wang, Charles E Murry, Ying Zheng
Vascularization and efficient perfusion are long-standing challenges in cardiac tissue engineering. Here we report engineered perfusable microvascular constructs, wherein human embryonic stem cell-derived endothelial cells (hESC-ECs) are seeded both into patterned microchannels and the surrounding collagen matrix. In vitro, the hESC-ECs lining the luminal walls readily sprout and anastomose with de novo-formed endothelial tubes in the matrix under flow. When implanted on infarcted rat hearts, the perfusable microvessel grafts integrate with coronary vasculature to a greater degree than non-perfusable self-assembled constructs at 5 days post-implantation...
February 4, 2019: Nature Communications
Anna Latysheva, Kyrre Eeg Emblem, Petter Brandal, Einar Osland Vik-Mo, Jens Pahnke, Kjetil Røysland, John K Hald, Andrés Server
PURPOSE: According to the revised World Health Organization (WHO) Classification of Tumors of the Central Nervous System (CNS) of 2016, oligodendrogliomas are now defined primarily by a specific molecular signature (presence of IDH mutation and 1p19q codeletion). The purpose of our study was to assess the value of dynamic susceptibility contrast MR imaging (DSC-MRI) and diffusion-weighted imaging (DWI) to characterize oligodendrogliomas and to distinguish them from astrocytomas. METHODS: Seventy-one adult patients with untreated WHO grade II and grade III diffuse infiltrating gliomas and known 1p/19q codeletion status were retrospectively identified and analyzed using relative cerebral blood volume (rCBV) and apparent diffusion coefficient (ADC) maps based on whole-tumor volume histograms...
February 2, 2019: Neuroradiology
Zhihua Si, Jinzhi Liu, Ke Hu, Yan Lin, Jie Liu, Aihua Wang
Recombinant tissue plasminogen activator (rt-PA) is the first-line drug for revascularization in acute cerebral infarction (ACI) treatment. In this study, an improved rat embolic middle cerebral artery occlusion model for ischaemic stroke was used and the rats were killed on the first, third and seventh day after model establishment. Increases in infarct volume were significantly less in the thrombolytic group than in the conventional group at every time-point. The microvascular density (MVD) in the thrombolytic group was significantly higher than that in the conventional group at every time-point, especially on the seventh day...
January 29, 2019: Journal of Cellular and Molecular Medicine
Kurt J Smith, Raden Argarini, Howard H Carter, Bryden C Quirk, Andrew Haynes, Louise H Naylor, Hamish McKirdy, Rodney W Kirk, Robert A McLaughlin, Daniel J Green
INTRODUCTION: Optical coherence tomography (OCT) is a novel high-resolution imaging technique capable of visualising in vivo structures at a resolution of ~10 μm. We have developed specialised OCT-based approaches that quantify diameter, speed and flow rate in human cutaneous microvessels. In this study we hypothesized that OCT-based microvascular assessments would possess comparable levels of reliability when compared to those derived using conventional laser Doppler flowmetry (LDF)...
January 24, 2019: Medicine and Science in Sports and Exercise
George A Parker, Na Li, Kyle Takayama, Ann M Farese, Thomas J MacVittie
Male rhesus macaques were subjected to partial-body irradiation at 10, 11, or 12 Gy with 5% bone marrow protection. Animals were euthanized when dictated by prospectively determined clinical parameters or at approximately 180 d following irradiation. Histological sections of lung and heart were stained with hematoxylin and eosin as well as a battery of histochemical and immunohistochemical stains. Histopathological alterations in the lung were centered on fibrosis, inflammation, and reactive/proliferative changes in pneumocytes...
March 2019: Health Physics
Yunkao Zeng, Dan Cao, Honghua Yu, Dawei Yang, Xuenan Zhuang, Yunyan Hu, Juan Li, Jing Yang, Qiaowei Wu, Baoyi Liu, Liang Zhang
AIMS: To investigate the function and the corresponding neurovascular structures in patients with diabetes without clinically detectable retinopathy. METHODS : Sixty-six patients with type 2 diabetes without retinopathy (NDR) and 62 healthy controls were recruited. The 16 and 32 Tds flicker electroretinography (ERG) was performed using a mydriasis-free, full-field flicker ERG recording device (RETeval). The vessel density (VD) of superficial capillary plexus (SCP) and deep capillary plexus (DCP), FD300 and ganglion cell complex (GCC) thickness in the macula were quantified using optical coherence tomography angiography (OCTA)...
January 23, 2019: British Journal of Ophthalmology
Jianping Song, Hongchun Shu, Ling Zhang, Jianping Xiong
OBJECTIVE: Angiogenesis plays a key role in the tumorigenesis and progression of colorectal cancer (CRC). In this study, we investigated the effect of long noncoding RNA (lncRNA) GAS5 on the angiogenesis, invasion, and metastasis of CRC, and the involvement of the Wnt/β-catenin signaling pathway. METHODS: CRC tissues and adjacent normal tissues were collected from 52 patients with CRC. GAS5 expression was determined in vivo and in vitro by real-time quantitative polymerase chain reaction (RT-qPCR)...
January 22, 2019: Journal of Cellular Biochemistry
Eduardo V Navajas, Nathan Schuck, Andrea Govetto, Handan Akil, Gavin Docherty, Morgan Heisler, Marinko V Sarunic, David Sarraf
PURPOSE: To quantitatively and qualitatively evaluate the microvascular and structural abnormalities associated with inner retinal dimpling after internal limiting membrane peeling for full-thickness macular holes using sequential en face optical coherence tomography (OCT) and OCT angiography. METHODS: Thirteen eyes of 13 patients with idiopathic full-thickness macular holes were enrolled in the study. Patients were treated with pars plana vitrectomy, internal limiting membrane peeling, and gas tamponade...
January 3, 2019: Retina
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