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Stress enduring cells

Siyao Wang, Qingyuan Tian, Shiqi Zhou, Dandan Mao, Liangbi Chen
BACKGROUND: Thermo-sensitive genetic male sterile (TGMS) lines have been widely used in two-line hybrid rice breeding. The two-line hybrids have increased rice yields substantially. However, the effect of environmental temperatures on the fertility conversion is still not fully clear. In this study, we performed a tandem mass tag (TMT)-based proteomic analysis on the anthers of the TGMS line AnnongS-1 grown under permissive (low) temperature (21 °C) and restrictive (high) temperature (> 26 °C) conditions in an attempt to explore the effect of temperature on the fertility of the male sterile line...
February 11, 2019: BMC Plant Biology
Almas Ashraf, Asghari Bano, Syed Abid Ali
RESEARCH RATIONALE: High temperature induces several proteins in plants to endure tolerance to high temperature shock. The fate of proteins synthesized in microbial cell or secreted in the culture media by the interacting microbe has not been fully elucidated. Present investigation aimed to characterize the PGPR isolated from the rhizosphere of wheat genotypes (differing in tolerance to high temperature stress) and to evaluate their performance as bioinoculant on wheat. METHODS APPLIED: Four bacterial strains viz...
February 7, 2019: Plant Biology
Ali M Fouad, Mahmoud M Gabr, Elsayed K Abdelhady, Mahmoud M Zakaria, Sherry M Khater, Amani M Ismail, Ayman F Refaie
Mesenchymal stem cells (MSCs) is a heterogeneous population. Muse cells is a rare pluripotent subpopulation within MSCs. This study aims to evaluate the pulirpotency and the ability of Muse cells to generate insulin producing cells (IPCs) after in vitro differentiation protocol compared to the non-Muse cells. Muse cells were isolated by FACSAria III cell sorter from adipose-derived MSCs and were evaluated for its pluripotency. Following in vitro differentiation, IPCs derived from Muse and non-Muse cells were evaluated for insulin production...
December 2018: Journal, Genetic Engineering & Biotechnology
Yuxiao Tang, Ruirui Qi, Huiwen Wu, Wencai Shi, Yan Xu, Min Li
The existence of sports anemia, induced by strenuous or long-term exercise and characterized by decreases of red blood cells (RBCs), hemoglobin and iron content, remains to be doubtful. To observe the effects of endurance exercise on RBCs and explain the underlying reason, we designed this study by observing RBCs parameters and iron metabolism in 8-weeks training rats and effects of iron supplement or protein supplement on RBCs. Results showed that erythrocyte counts, hematocrit, mean corpuscular volume and hemoglobin content decreased while RBC distribution width increased in exercised rats at later stage during 8 weeks training...
March 2019: Journal of Trace Elements in Medicine and Biology
Christine L Childers, Kenneth B Storey
The African clawed frog, Xenopus laevis endures whole body dehydration which can increase its reliance on anaerobic glycolysis for energy production. This makes the regulation of the terminal enzyme of glycolysis, lactate dehydrogenase (LDH), crucial to stress survival. We investigated the enzymatic properties and posttranslational modification state of purified LDH from the skeletal muscle of control and dehydrated (30% total body water loss) X. laevis. LDH from the muscle of dehydrated frogs showed a 93% reduction in phosphorylation on threonine residues and an 80% reduction of protein nitrosylation...
January 10, 2019: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Divya Rathi, Saurabh Gayali, Akanksha Pareek, Subhra Chakraborty, Niranjan Chakraborty
This study highlights dehydration-mediated temporal changes in physicochemical, transcriptome and metabolome profiles indicating altered gene expression and metabolic shifts, underlying endurance and adaptation to stress tolerance in the marginalized crop, grasspea. Grasspea, often regarded as an orphan legume, is recognized to be fairly tolerant to water-deficit stress. In the present study, 3-week-old grasspea seedlings were subjected to dehydration by withholding water over a period of 144 h. While there were no detectable phenotypic changes in the seedlings till 48 h, the symptoms appeared during 72 h and aggravated upon prolonged dehydration...
January 10, 2019: Planta
F B Litvin, T M Bruk, S V Klochkova, A I Kalosha, D B Nikityuk
Specialized sports nutrition is one of the most important factors in the extension of the functional potential of athletes, providing adaptive resistance to physical stress, which determines the high physical performance and prolongs athletic longevity of the athletes. The study involved 30 skiers-racers (the average age of 19.5±1.8 years). 12 skiers of the main group within 21 days consumed a specialized food product, obtained on the basis of fermented milk whey containing amino acids, several vitamins, minerals and trace elements, live culture of lactic acid bacteria: L...
2018: Voprosy Pitaniia
Leydi Natalia Vittori, Andrea Tarozzi, Pasqualino Maietta Latessa
The interest about circulating cell-free DNA (cfDNA) concentration increased from several years because of its correlation with various conditions like osteoarthritis, cancers, stroke, and sepsis; recently it has become an important marker for overtraining syndrome or performance diagnostics.Several studies have demonstrated that cfDNA increases in vigorous and exhausting exercise but also endurance exercise. Acute effect of exercise on cfDNA concentration seems to be correlated to stress factor, while chronic effect is associated with necrosis and apoptosis...
2019: Methods in Molecular Biology
Benjamin Chatel, Laurent A Messonnier, Christophe Vilmen, Monique Bernard, Vincent Pialoux, David Bendahan
NEW FINDINGS: The aim of this study was to evaluate the potential beneficial effects of endurance training during an ischemia/reperfusion paradigm in sickle cell disease mice. Endurance training would not reverse the metabolic defects induced by a simulated vaso-occlusive crisis in sickle cell mice, whether it is with regard to intramuscular acidosis, mitochondrial dysfunction or anatomical properties. Our results suggest that endurance training would reduce vaso-occlusive crisis number rather than the complications related to vaso-occlusive crisis...
December 22, 2018: Experimental Physiology
Roy G Cutler, Simonetta Camandola, Neil H Feldman, Jeong Seon Yoon, James B Haran, Sandro Arguelles, Mark P Mattson
Among mammals, there is a positive correlation between serum uric acid (UA) levels and life span. Humans have high levels of UA because they lack a functional urate oxidase (UOX) enzyme that is present in shorter lived mammals. Here, we show that male and female mice with UOX haploinsufficiency exhibit an age-related elevation of UA levels, and that the life span of female but not male UOX+/- mice is significantly increased compared to wild-type mice. Serum UA levels are elevated in response to treadmill exercise in UOX+/- mice, but not wild-type mice, and the endurance of the UOX+/- mice is significantly greater than wild-type mice...
December 12, 2018: Neurobiology of Aging
Alba Signes, Raffaele Cerutti, Anna S Dickson, Cristiane Benincá, Elizabeth C Hinchy, Daniele Ghezzi, Rosalba Carrozzo, Enrico Bertini, Michael P Murphy, James A Nathan, Carlo Viscomi, Erika Fernandez-Vizarra, Massimo Zeviani
Loss-of-function mutations in APOPT1 , a gene exclusively found in higher eukaryotes, cause a characteristic type of cavitating leukoencephalopathy associated with mitochondrial cytochrome c oxidase (COX) deficiency. Although the genetic association of APOPT1 pathogenic variants with isolated COX defects is now clear, the biochemical link between APOPT1 function and COX has remained elusive. We investigated the molecular role of APOPT1 using different approaches. First, we generated an Apopt1 knockout mouse model which shows impaired motor skills, e...
December 14, 2018: EMBO Molecular Medicine
David Montero, Carsten Lundby
Hypervolemia is a hallmark of endurance training (ET) and manifests by similar elevations in plasma (PV) and red blood cell volume (RBCV) so that hematocrit largely remains unaltered following weeks/months of training. While the mechanisms facilitating PV expansion with ET have been previously reviewed extensively this is not the case for RBCV. Endurance champions may have 40% more RBCV than controls and RBCV may increase up to 10% following months of regular exercise training in healthy individuals. Such adaptations are the main factor leading to concomitant changes in maximal oxygen uptake...
December 13, 2018: Comprehensive Physiology
Won Ho Kim, Chi-Ho Lee, Jung-Hwa Han, Sujin Kim, Seong Yong Kim, Jae Hyang Lim, Kwon Moo Park, Duk Seop Shin, Chang-Hoon Woo
It has been well established that HMG-CoA reductase inhibitors (statins) cause adverse side effects in skeletal muscle ranging from mild to fatal myotoxicity upon dose, drug interaction, and exercise. However, the underlying mechanisms by which statins induce myotoxicity have not been fully addressed. Recent reports showed that statins induce endoplasmic reticulum (ER) stress and cell death in immune cells and myoblasts in vitro. Therefore, the goal of study is to investigate the molecular mechanism by which statins induce skeletal muscle cell death and myopathy via the regulation of ER stress...
December 7, 2018: Biochemical and Biophysical Research Communications
Eriko Toyoda, Masato Sato, Takumi Takahashi, Miki Maehara, Yoshihiko Nakamura, Genya Mitani, Tomonori Takagaki, Kosuke Hamahashi, Masahiko Watanabe
Introduction: Cartilage regeneration is a promising therapy for restoring joint function in patients with cartilage defects. The limited availability of autologous chondrocytes or chondrogenic progenitor cells is an obstacle to its clinical application. We investigated the existence and chondrogenic potential of synovial membrane-derived multilineage-differentiating stress-enduring (Muse)-like cells as an alternative cell source for cartilage regeneration. Methods: Cells positive for stage-specific embryonic antigen-3 (SSEA-3), a marker of Muse cells, were isolated from the synovial membranes of 6 of 8 patients (median age, 53...
June 2019: Regenerative therapy
Weixiu Ji, Linjia Wang, Shiyi He, Lu Yan, Tieying Li, Jianxiong Wang, Ah-Ng Tony Kong, Siwang Yu, Ying Zhang
BACKGROUND: Hypoxia training enhances the endurance capacity of athletes. This response may in part be attributed to the hypoxia-induced increase in antioxidant capacity in skeletal muscles. Nuclear factor erythroid 2-related factor 2 (Nrf2), a key transcription factor which regulates the expression of genes via binding to the antioxidant-response element (ARE) of these genes, plays a crucial role in stimulating the body's defense system and potentially responds to hypoxia. Meanwhile, hypoxia-inducible factor-1α (HIF-1α) is an important player in protecting cells from hypoxic stress...
2018: PloS One
Katsuhiro Hosoyama, Yoshikatsu Saiki
The aorta is a well-organized, multilayered structure comprising several cell types, namely, endothelial cells (ECs), vascular smooth muscle cells (VSMCs), and fibroblasts, as well as an extracellular matrix (ECM), which includes elastic and collagen fibers. Aortic aneurysms (AAs) are defined as progressive enlargements of the aorta that carries an incremental risk of rupture as the diameter increases over time. The destruction of the aortic wall tissue is triggered by atherosclerosis, inflammation, and oxidative stress, leading to the activation of matrix metalloproteinases (MMPs), and inflammatory cytokines and chemokines, resulting in the loss of the structural back bone of VSMCs, ECM, and ECs...
2018: Advances in Experimental Medicine and Biology
Takeshi Yamauchi, Kenshi Yamasaki, Kenichiro Tsuchiyama, Setsuya Aiba
The skin composes physiological and chemical barrier and renews skin component cells throughout the human life. Melanocytes locate in the basal layer of the epidermis and produce melanin to protect the skin from ultraviolet. Melanin plays key roles in determining human skin and hair color. Melanocyte dysfunction observed in albinism and vitiligo not only causes cosmetic problems but also increases risk of skin cancer. As rejuvenate therapy, embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have been reported to generate melanocytes...
2018: Advances in Experimental Medicine and Biology
Satoshi S Nishizuka, Yuji Suzuki, Hirokatsu Katagiri, Yasuhiro Takikawa
Cellular compensation from extrahepatic resources is expected to improve the prognosis of liver diseases. Currently, liver dysfunction is treated by a variety of modalities including drugs, cytokines, vascular interventions, energy devices, surgery, and liver transplantation; however, in recent years there have been few significant advancements in treatment efficacy. A next-generation therapeutic strategy for liver disease, cellular compensatory therapy (i.e., cell therapy), is now being considered for clinical practice...
2018: Advances in Experimental Medicine and Biology
Satoshi Kuroda, Masaki Koh, Emiko Hori, Yumiko Hayakawa, Takuya Akai
Multilineage-differentiating stress enduring (Muse) cells are one of the most promising donor cells for cell therapy against ischemic stroke, because they can differentiate into any type of cells constructing the central nervous system (CNS), including the neurons. They can easily be isolated from the bone marrow stromal cells (BMSCs), which may also contribute to functional recovery after ischemic stroke as donor cells. In this chapter, we concisely review their biological features and then future perspective of Muse cell transplantation for ischemic stroke...
2018: Advances in Experimental Medicine and Biology
Kuniyasu Niizuma, Cesar V Borlongan, Teiji Tominaga
Stroke is defined as a sudden onset of neurologic deficits arising from cerebrovascular complications. It is the second common cause of death around the world and the major cause of disability. Because brain is an organ with complicated neural networks and neurons are highly differentiated, it has been traditionally considered to possess a limited potential for regeneration. The number of stroke patients is increasing, and stroke represents a serious problem from the viewpoint of the national medical economy...
2018: Advances in Experimental Medicine and Biology
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