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Gian Carlo Tenore, Ester Pagano, Stefania Lama, Daniela Vanacore, Salvatore Di Maro, Maria Maisto, Raffaele Capasso, Francesco Merlino, Francesca Borrelli, Paola Stiuso, Ettore Novellino
Under physiological conditions, the small intestine represents a barrier against harmful antigens and pathogens. Maintaining of the intestinal barrier depends largely on cell⁻cell interactions (adherent-junctions) and cell⁻matrix interactions (tight-junctions). Inflammatory bowel disease is characterized by chronic inflammation, which induces a destructuring of the architecture junctional epithelial proteins with consequent rupture of the intestinal barrier. Recently, a peptide identified by Bubalus bubalis milk-derived products (MBCP) has been able to reduce oxidative stress in intestinal epithelial cells and erythrocytes...
March 13, 2019: Nutrients
Maria J Mosqueira, Ramona Marasco, Marco Fusi, Grégoire Michoud, Giuseppe Merlino, Ameur Cherif, Daniele Daffonchio
Highly productive conventional agroecosystems are spatially embedded in resource-homogeneous systems and count on generally nutrient-rich soils. On the contrary, desert oases are isolated, the soil is relatively poor, but yet productivity is similar to conventional agroecosystems. Soil dominates over plant as the main factor shaping root-associated microbiomes in conventional agroecosystems. We hypothesize that in desert oasis, the environmental discontinuity, the resource paucity and limited microbial diversity of the soil make the plant a prevailing factor...
March 11, 2019: Scientific Reports
Nicholas Castaneda, Myeongsang Lee, Héctor J Rivera-Jacquez, Ryan R Marracino, Theresa R Merlino, Hyeran Kang
The cellular environment is crowded with high concentrations of macromolecules that significantly reduce accessible volume for biomolecular interactions. Reductions in cellular volume can generate depletion forces that affect protein assembly and stability. The mechanical and structural properties of actin filaments play critical roles in various cellular functions including structural support, cell movement, division, and intracellular transport. Although the effects of molecular crowding on actin polymerization have been shown, how crowded environments affect filament mechanics and structure is unknown...
February 28, 2019: Journal of Physical Chemistry. B
Giampiero Ferraguti, Lucia Merlino, Gemma Battagliese, Maria Grazia Piccioni, Greta Barbaro, Valentina Carito, Marisa Patrizia Messina, Bruna Scalese, Giovanna Coriale, Marco Fiore, Mauro Ceccanti
Fetal alcohol spectrum disorders (FASDs) are a group of negative conditions occurring in children exposed to alcohol during gestation. The early discovery of FASD is crucial for mother and infant follow-ups. In this study, we investigated in pregnant women the association between urine ethylglucuronide (EtG-a biomarker of alcohol drinking) and indicators of the physical characteristics of FASD by prenatal ultrasound in the second trimester of gestation. We also correlated these data with the AUDIT-C, T-ACE/TACER-3, TWEAK, and food habit diary, screening questionnaires used to disclose alcohol drinking during pregnancy...
February 27, 2019: Addiction Biology
M Locritani, S Merlino, M Abbate
This paper provides a quantitative assessment of students' attitude and behaviors towards marine litter before and after their participation to SEACleaner, an educational and citizen science project devoted to monitor macro- and micro-litter in an Area belonging to "Pelagos Sanctuary" (Mediterranean Sea). This approach produced interesting outcomes both for the research sector of marine pollution and environmental monitoring, as well for the scientific and environmental education. Here we focus on citizen science as an effective vector for raising young people awareness of marine litter and fostering sound behaviors...
March 2019: Marine Pollution Bulletin
Daniele Florio, Anna Maria Malfitano, Sarah Di Somma, Carolin Mügge, Wolfgang Weigand, Giarita Ferraro, Ilaria Iacobucci, Maria Monti, Giancarlo Morelli, Antonello Merlino, Daniela Marasco
Platinum(II) complexes with different cinnamic acid derivatives as ligands were investigated for their ability to inhibit the aggregation process of amyloid systems derived from Aβ, Yeast Prion Protein Sup35p and the C-terminal domain of nucleophosmin 1. Thioflavin T binding assays and circular dichroism data indicate that these compounds strongly inhibit the aggregation of investigated peptides exhibiting IC50 values in the micromolar range. MS analysis confirms the formation of adducts between peptides and Pt(II) complexes that are also able to reduce amyloid cytotoxicity in human SH-SY5Y neuroblastoma cells...
February 14, 2019: International Journal of Molecular Sciences
Babar Bashir, Dante J Merlino, Jeffrey A Rappaport, Esteban Gnass, Juan P Palazzo, Ying Feng, Eric R Fearon, Adam E Snook, Scott A Waldman
Colorectal cancers (CRCs) initiate through distinct mutations, including in APC pathway components leading to tubular adenomas (TAs); in BRAF, with epigenetic silencing of CDX2, leading to serrated adenomas (SAs); and in the DNA mismatch repair machinery driving microsatellite instability (MSI). Transformation through the APC pathway involves loss of the hormone GUCA2A that silences the tumor suppressing receptor GUCY2C. Indeed, oral hormone replacement is an emerging strategy to reactivate GUCY2C and prevent CRC initiation and progression...
February 1, 2019: Human Pathology
Giarita Ferraro, Tiziano Marzo, Maria Elena Cucciolito, Francesco Ruffo, Luigi Messori, Antonello Merlino
Stable five-coordinate Pt(II) complexes have been highlighted as a promising and original platform for the development of new cytotoxic drugs. Their interaction with proteins has been scarcely studied. Here, the reactivity of the five-coordinate Pt(II) compound [Pt(I)(Me) (dmphen)(olefin)] (Me = methyl, dmphen = 2,9-dimethyl-1,10-phenanthroline, olefin = dimethylfumarate) with the model proteins hen egg white lysozyme (HEWL) and bovine pancreatic ribonuclease (RNase A) has been investigated by X-ray crystallography and electrospray ionization mass spectrometry...
January 26, 2019: International Journal of Molecular Sciences
Stephanie Merlino-Barr, Sharon Groh-Wargo
Expressed breast milk (EBM) is the gold standard of infant nutrition, but is not always available for use for preterm infants in the NICU setting. Donor breast milk (DBM) is often a preferred alternative for preterm and very low birth weight (VLBW) infants when maternal milk is not available. This article discusses the composition of DBM, reviews its advantages compared to formula, discusses challenges related to its long-term use, and identifies strategies to utilize DBM in the context of total nutritional management of preterm and VLBW infants...
January 2019: Neonatal Network: NN
Valentina Maria Merlino, Danielle Borra, Tibor Verduna, Stefano Massaglia
Due to an error during production, the order of the first and last names of the authors are incorrect in the published paper [...].
January 22, 2019: Veterinary Sciences
Ganna Petruk, Daria Maria Monti, Giarita Ferraro, Andrea Pica, Luigi D'Elia, Francesca Pane, Angela Amoresano, Julien Furrer, Konrad Kowalski, Antonello Merlino
The effects of the encapsulation of the cytotoxic dinuclear trithiolato-bridged arene Ru complex [(η6-p-MeC6H4Pri)2Ru2(μ2-S-p-C6H4But)3]Cl (DiRu-1) within the apoferritin (AFt) nanocage were investigated. The DiRu-1-AFt nanocarrier was characterized by UV-Vis spectroscopy, ICP MS, CD and X-ray crystallography. In contrast to previously reported Au- and Pt- based drug-loaded AFt carriers, no direct interactions between DiRu-1 and AFt were evidenced. DiRu-1-AFt is cytotoxic towards immortalized murine fibroblast BALB/c-3T3 transformed with SV40 virus (SVT2) and human epidermoid carcinoma A431 malignant cells and exhibits a moderate selectivity for these cancer cells over the normal BALB/c-3T3 cells...
January 23, 2019: ChemMedChem
Amedeo Minichino, Grazia Rutigliano, Sergio Merlino, Cathy Davies, Dominic Oliver, Andrea De Micheli, Rashmi Patel, Philip McGuire, Paolo Fusar-Poli
BACKGROUND: Patients with acute and transient psychotic disorders (ATPDs) are by definition remitting, but have a high risk of developing persistent psychoses, resembling a subgroup of individuals at Clinical High Risk for Psychosis (CHR-P). Their pathways to care, treatment offered and long-term clinical outcomes beyond risk to psychosis are unexplored. We conducted an electronic health record-based retrospective cohort study including patients with ATPDs within the SLaM NHS Trust and followed-up to 8 years...
January 15, 2019: European Psychiatry: the Journal of the Association of European Psychiatrists
Raffaele Addeo, Massimo Ghiani, Francesco Merlino, Filippo Ricciardiello, Michele Caraglia
No abstract text is available yet for this article.
January 17, 2019: Expert Opinion on Biological Therapy
Kerrie L Marie, Glenn Merlino
In this issue, Weeraratna and colleagues demonstrate that observed differences in melanoma aggressiveness in younger versus older patients can be explained not just by cell-intrinsic alterations over time, but by age-dependent changes in fibroblasts and the extracellular matrix they help create. Their findings identify novel cellular targets for melanoma therapy, as well as candidate prognostic biomarkers to better inform clinical decisions for patients with melanoma. See related article by Kaur et al., p. 64 ...
January 2019: Cancer Discovery
Francesco Merlino, Etienne Billard, Ali Munaim Yousif, Salvatore Di Maro, Diego Brancaccio, Luigi Abate, Alfonso Carotenuto, Rosa Bellavita, Roberta d'Emmanuele di Villa Bianca, Paolo Santicioli, Luciana Marinelli, Ettore Novellino, Terence Hébert, William D Lubell, David Chatenet, Paolo Grieco
In accordance with their common but also divergent physiological actions, human urotensin II ( hU-II, 1) and urotensin II-related peptide (URP, 2) could stabilize specific urotensin II receptor (UTR) conformations, thereby activating different signalling pathways, a feature referred to as biased agonism or functional selectivity. Sequential N-methylation of the amides in the conserved core sequence of 1, 2 and fragment U-II4-11 (3) shed light on structural requirements involved in their functional selectivity...
January 7, 2019: Journal of Medicinal Chemistry
Marco Caterino, Mona Herrmann, Antonello Merlino, Claudia Riccardi, Daniela Montesarchio, Maria A Mroginski, Domenica Musumeci, Francesco Ruffo, Luigi Paduano, Peter Hildebrandt, Jacek Kozuch, Alessandro Vergara
The RuIII -based prodrug AziRu efficiently binds to proteins, but the mechanism of its release is still disputed. Herein, in order to test the hypothesis of a reduction-mediated Ru release from proteins, a Raman-assisted crystallographic study on AziRu binding to a model protein (hen egg white lysozyme), in two different oxidation states, RuII and RuIII , was carried out. Our results indicate Ru reduction, but the Ru release upon reduction is dependent on the reducing agent. To better understand this process, a pH-dependent, spectroelectrochemical surface-enhanced Raman scattering (SERS) study was performed also on AziRu-functionalized Au electrodes as a surrogate and simplest model system of RuII - and RuIII -based drugs...
January 7, 2019: Inorganic Chemistry
Daria Putignano, Antonio Clavenna, Rita Campi, Maria Paola Canevini, Aglaia Vignoli, Dina Battino, Ettore Beghi, Emilio Perucca, Angela Bortolotti, Ida Fortino, Luca Merlino, Maurizio Bonati
Healthcare administrative databases of Italy's Lombardy Region were analyzed with the aim to assess perinatal outcomes and healthcare resource utilization during the first year of life in infants exposed to antiepileptic drugs (AEDs) during pregnancy. Drug prescriptions dispensed in the 12 months before delivery to women, who delivered between 2005 and 2011, were analyzed. Neonates were classified as cases if exposed to AEDs, and each case was randomly matched to seven controls. No significant differences were observed in the risk of congenital malformations between 526 cases and 3682 controls except for valproic acid (odds ratio (OR): 2...
December 29, 2018: Epilepsy & Behavior: E&B
Helen T Michael, Cari Graff-Cherry, Sung Chin, Corinne Rauck, Amelework D Habtemichael, Patricia Bunda, Tunde Smith, Maria M Campos, Kapil Bharti, Heinz Arnheiter, Glenn Merlino, Chi-Ping Day
Purpose: Complete deficiency of microphthalmia transcription factor (MITF) in Mitfmi-vga9/mi-vga9 mice is associated with microphthalmia, retinal dysplasia, and albinism. We investigated the ability of dopachrome tautomerase (DCT) promoter-mediated inducible ectopic expression of Mitf-M to rescue these phenotypic abnormalities. Methods: A new mouse line was created with doxycycline-inducible ectopic Mitf-M expression on an Mitf-deficient Mitfmi-vga9 background (DMV mouse)...
December 3, 2018: Investigative Ophthalmology & Visual Science
Helen T Michael, Cari Graff-Cherry, Sung Chin, Corinne Rauck, Amelework D Habtemichael, Patricia Bunda, Tunde Smith, Maria M Campos, Kapil Bharti, Heinz Arnheiter, Glenn Merlino, Chi-Ping Day
Purpose: Complete deficiency of microphthalmia transcription factor (MITF) in Mitfmi-vga9/mi-vga9 mice is associated with microphthalmia, retinal dysplasia, and albinism. We investigated the ability of dopachrome tautomerase (DCT) promoter-mediated inducible ectopic expression of Mitf-M to rescue these phenotypic abnormalities. Methods: A new mouse line was created with doxycycline-inducible ectopic Mitf-M expression on an Mitf-deficient Mitfmi-vga9 background (DMV mouse)...
December 3, 2018: Investigative Ophthalmology & Visual Science
Giovanni Merlino, Massimo Sponza, Gian Luigi Gigli, Simone Lorenzut, Alessandro Vit, Vladimir Gavrilovic, Andrea Pellegrin, Daniela Cargnelutti, Mariarosaria Valente
Endovascular therapy (EVT) represents the gold standard treatment in patients affected by acute ischemic stroke (AIS) due to large vessel occlusion (LVO). Prior antiplatelet (APT) therapy might increase the risk of bleeding and modify the clinical outcome of AIS patients treated with EVT. Thus, we compared effectiveness and safety of EVT in Caucasian patients with and without previous use of APT agents. We recruited consecutive patients admitted to Udine University Hospital with AIS undergoing EVT from January 2015 to December 2017...
December 5, 2018: Journal of Clinical Medicine
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