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Jin Song, Shannath L Merbs, Lori J Sokoll, Daniel W Chan, Zhen Zhang
Background: Approximately 50% of uveal melanoma (UM) patients develop metastases preferentially in the liver leading to death within 15 months. Currently, there is no effective treatment for metastatic UM, in part because the tumor burden is typically high when liver metastases are detected through abnormal liver function tests (LFTs) or imaging studies. The use of LFTs results followed by diagnostic tests has high specificity and predictive values but low sensitivity, and better tests are needed for early diagnosis of the primary tumor as well as its metastatic spread...
2019: Clinical Proteomics
M A A Vogel, P Wang, F G Bouwman, N Hoebers, E E Blaak, J Renes, E C Mariman, G H Goossens
Body fat distribution is an important determinant of cardiometabolic health. Lower-body adipose tissue (AT) has protective characteristics as compared to upper-body fat, but the underlying depot-differences remain to be elucidated. Here, we compared the proteome and morphology of abdominal and femoral AT. Paired biopsies from abdominal and femoral subcutaneous AT were taken from eight overweight/obese (BMI ≥ 28 kg/m2 ) women with impaired glucose metabolism after an overnight fast. Proteins were isolated and quantified using liquid chromatography-mass spectrometry, and protein expression in abdominal and femoral subcutaneous AT was compared...
March 12, 2019: Scientific Reports
Nicola Chiarelli, Giulia Carini, Nicoletta Zoppi, Marco Ritelli, Marina Colombi
Classical Ehlers-Danlos syndrome (cEDS) is a dominant inherited connective tissue disorder mainly caused by mutations in the COL5A1 and COL5A2 genes encoding type V collagen (COLLV), which is a fibrillar COLL widely distributed in a variety of connective tissues. cEDS patients suffer from skin hyperextensibility, abnormal wound healing/atrophic scars, and joint hypermobility. Most of the causative variants result in a non-functional COL5A1 allele and COLLV haploinsufficiency, whilst COL5A2 mutations affect its structural integrity...
2019: PloS One
Hai Zhong, Xiang Li, Junhua Zhang, Xu Wu
BACKGROUND: Gastric tumors generally have a poor prognosis and molecular markers to improve early detection and predict outcomes are greatly needed. The present study reports that periostin (POSTN), a secretory protein that can alter the remodeling of the extracellular matrix, is highly expressed in gastric tumors. MATERIALS AND METHODS: Gastric tissues were collected from patients at the Department of Thoracic Surgery/Huiqiao Medical Center, Nanfang Hospital, Southern Medical University...
January 13, 2019: Journal of Cellular Biochemistry
Hideki Kanamaru, Fumihiro Kawakita, Fumi Nakano, Yoichi Miura, Masato Shiba, Ryuta Yasuda, Naoki Toma, Hidenori Suzuki
Delayed cerebral ischemia (DCI) is a serious complication of aneurysmal subarachnoid hemorrhage (SAH). Matricellular protein periostin (POSTN) has been found to be upregulated and linked with early brain injury after experimental SAH. The aim of the present study was to investigate the relationship between plasma POSTN levels and various clinical factors including serum levels of C-reactive protein (CRP), an inflammatory marker, in 109 consecutive SAH patients whose POSTN levels were measured at days 1-12 after aneurysmal obliteration...
January 11, 2019: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
Fazal Ilahi Jamesha, Arun Parappa Maradi, Koshy Chithresan, Srihari Janakiram, Praveen Krishna Maddur, Rajesh Rangaraju
Background: In both states of health and disease, the integrity of connective tissue along with regulation in formation of bones are well maintained by periostin (POSTN) which is a matricellular protein secreted by fibroblasts. The present study aimed to assess the gingival crevicular fluid (GCF) POSTN levels in patients with chronic periodontitis (CP) and aggressive periodontitis and to compare them with that of healthy controls. Materials and Methods: A total of 39 individuals were recruited and allocated into the healthy group, and two periodontitis groups (the chronic and the aggressive types) (13 in each group)...
November 2018: Journal of Indian Society of Periodontology
C Alfieri, A Regalia, G Moroni, D Cresseri, F Zanoni, M Ikehata, P Simonini, M P Rastaldi, G Tripepi, C Zoccali, C Chatziantoniou, Piergiorgio Messa
Renal biopsy (RBx) informs about kidney transplantation (KTx) prognosis. In our observational study the prevalence of histological anomalies and the prognostic role of CD45, vimentin (VIM) and periostin (POSTN) in KTx-RBx have been evaluated. One hundred forty-six KTx-RBx (2009-2012) were analysed for general histology and in immunohistochemistry for CD45, VIM and POSTN. Clinical data of the 146-KTx patients were collected at the RBx time (T0), 6 and 12 months before and after RBx. Follow-up time was 21 ± 14 months...
January 10, 2019: Journal of Nephrology
Kelvin K Wong, Robert Rostomily, Stephen T C Wong
This study aims to discover genes with prognostic potential for glioblastoma (GBM) patients' survival in a patient group that has gone through standard of care treatments including surgeries and chemotherapies, using tumor gene expression at initial diagnosis before treatment. The Cancer Genome Atlas (TCGA) GBM gene expression data are used as inputs to build a deep multilayer perceptron network to predict patient survival risk using partial likelihood as loss function. Genes that are important to the model are identified by the input permutation method...
January 8, 2019: Cancers
Keisuke Imoto, Muneyoshi Okada, Hideyuki Yamawaki
Pulmonary arterial hypertension (PAH) leads to lethal right ventricular failure (RVF). Periostin ( POSTN ) mRNA expression is increased in right ventricles (RVs) of monocrotaline (MCT)-induced PAH model rats. However, the pathophysiological role of POSTN in RVF has not been clarified. We investigated the effects of POSTN on inducible nitric oxide (NO) synthase (iNOS) expression and NO production, which causes cardiac dysfunction, in right ventricular fibroblasts (RVFbs). Male Wistar rats were intraperitoneally injected with MCT (60 mg/kg) or saline...
December 24, 2018: International Journal of Molecular Sciences
Yu-Wen Huang, Ming-Fu Chiang, Che-Sheng Ho, Pi-Lien Hung, Mei-Hsin Hsu, Tsung-Han Lee, Lichieh Julie Chu, Hsuan Liu, Petrus Tang, Wailap Victor Ng, Dar-Shong Lin
Aging is a complex biological process. A study of pyrroline-5-carboxylate reductase 1 (PYCR1) deficiency, which causes a progeroid syndrome, may not only shed light on its genetic contribution to autosomal recessive cutis laxa (ARCL) but also help elucidate the functional mechanisms associated with aging. In this study, we used RNA-Seq technology to examine gene expression changes in primary skin fibroblasts from healthy controls and patients with PYCR1 mutations. Approximately 22 and 32 candidate genes were found to be up- and downregulated, respectively, in fibroblasts from patients...
December 2018: Aging and Disease
Qinghang Meng, Bidur Bhandary, Md Shenuarin Bhuiyan, Jeanne James, Hanna Osinska, Iñigo Valiente-Alandi, Kritton Shay-Winkler, James Gulick, Jeffery D Molkentin, Burns C Blaxall, Jeffrey Robbins
RATIONALE: Hypertrophic cardiomyopathy occurs with a frequency of about 1 in 500 people. Approximately 30% of those affected carry mutations within the gene encoding cMyBP-C (cardiac myosin binding protein C). Cardiac stress, as well as cMyBP-C mutations, can trigger production of a 40kDa truncated fragment derived from the amino terminus of cMyBP-C (Mybpc340kDa ). Expression of the 40kDa fragment in mouse cardiomyocytes leads to hypertrophy, fibrosis, and heart failure. Here we use genetic approaches to establish a causal role for excessive myofibroblast activation in a slow, progressive genetic cardiomyopathy-one that is driven by a cardiomyocyte-intrinsic genetic perturbation that models an important human disease...
December 7, 2018: Circulation Research
Maria Bogdanova, Aleksandra Kostina, Katarina Zihlavnikova Enayati, Arsenii Zabirnyk, Anna Malashicheva, Kåre-Olav Stensløkken, Gareth John Sullivan, Mari-Liis Kaljusto, John-Peder Escobar Kvitting, Anna Kostareva, Jarle Vaage, Arkady Rutkovskiy
Background: Aortic valve calcification is an active proliferative process, where interstitial cells of the valve transform into either myofibroblasts or osteoblast-like cells causing valve deformation, thickening of cusps and finally stenosis. This process may be triggered by several factors including inflammation, mechanical stress or interaction of cells with certain components of extracellular matrix. The matrix is different on the two sides of the valve leaflets. We hypothesize that inflammation and mechanical stress stimulate osteogenic differentiation of human aortic valve interstitial cells (VICs) and this may depend on the side of the leaflet...
2018: Frontiers in Physiology
Valentine N Nfonsam, Landry E Nfonsam, Debbie Chen, Pamela N Omesiete, Alejandro Cruz, Raymond B Runyan, Jana Jandova
BACKGROUND: About 1.2 million new cases of colon cancer (CC) and 0.6 million deaths are reported every year, establishing CC as an important contributor to worldwide cancer morbidity and mortality. Although the overall incidence and mortality of CC have declined over the past 3 decades, the number of early-onset colon cancer ([EOCC], patients <50 y old) continues to rise alarmingly. These young patients are often diagnosed at a more advanced stage and tend to have poor survival. Our recently published data showed that the cartilage oligomeric matrix protein (COMP) is overexpressed in early-onset colon cancer patients...
January 2019: Journal of Surgical Research
Qing Zhang, Chen Wang, William A Cliby
OBJECTIVE: Mesenchymal (MES) subtype of high-grade serous ovarian cancer (HGSOC) is associated with worse outcomes including survival and resectability compared with other molecular subtypes. Molecular subtypes have historically been derived from 'tumor', consisting of both cancer and stromal cells. We sought to determine the origins of multiple MES subtype gene signatures in HGSOC. METHODS: Fifteen patients with MES subtype of HGSOC diagnosed between 2010 and 2013 were identified...
November 15, 2018: Gynecologic Oncology
Rui Zhang, Xia-Hui Lin, Min Ma, Jie Chen, Jun Chen, Dong-Mei Gao, Jie-Feng Cui, Rong-Xin Chen
BACKGROUND: Incomplete thermal ablation may induce invasiveness of hepatocellular carcinoma (HCC). Here, we investigated whether activated hepatic stellate cells (HSCs) would accelerate the progression of residual HCC after sublethal heat treatment, and thus sought to identify the potential targets. METHODS: Hepatocellular carcinoma cells were exposed to sublethal heat treatment and then cultured with the conditioned medium from activated HSCs (HSC-CM). The cell proliferation, migration, invasion and parameters of epithelial-mesenchymal transition (EMT) were analyzed...
November 6, 2018: Journal of Translational Medicine
Pornrat Kongkavitoon, Patcharavadee Butta, Anapat Sanpavat, Pattarasinee Bhattarakosol, Pattarin Tangtanatakul, Benjawan Wongprom, Pisit Tangkijvanich, Nattiya Hirankarn, Tanapat Palaga
Notch signaling is involved in both differentiation of hepatocyte progenitors and hepatocellular carcinoma (HCC). The mechanism whereby Notch signaling regulates cellular transformation in hepatocytes is still controversial. This study investigated the impact of overexpressing truncated intracellular Notch1 (NICD1) on transcriptomic profiles of immortalized human hepatocytes. RNA sequencing and gene ontology enrichment analysis revealed that extracellular matrix organization and hyaluronan biosynthesis process gene sets are among those affected by Notch hyperactivation...
November 30, 2018: Biochemical and Biophysical Research Communications
Yuqing Mo, Kan Zhang, Yuchen Feng, Lingling Yi, Yuxia Liang, Wenliang Wu, Jian-Ping Zhao, Zhenxiang Zhang, Yongjian Xu, Qinghua Hu, Jianguo He, Guohua Zhen
Serine peptidase inhibitor, clade B, member 10 (SERPINB10) expression is increased in IL-13-stimulated human bronchial epithelial cells and in a murine model of allergic airway inflammation. However, the role of SERPINB10 in asthma remains unknown. We examined the association between epithelial SERPINB10 expression and airway eosinophilia in subjects with asthma and the role of Serpinb10 in allergic airway inflammation in animal model. Epithelial SERPINB10 mRNA and protein expression were markedly increased in subjects with asthma ( n = 60) compared with healthy controls ( n = 25)...
November 1, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
Pooja A Sankardas, Vamsi Lavu, Bhaskar V K S Lakakula, Suresh R Rao
AIM: The aim of the present study was to determine and compare the expression profile of periostin (POSTN), sclerostin (SOST), receptor activator nuclear factor-κB (RANK), and RANK ligand (RANKL) genes in gingival tissue samples collected from healthy gingiva (control) and severe chronic periodontitis sites. METHODS: Fifty systemically-healthy individuals was enrolled in the present case-control study. Gingival tissue samples were obtained from healthy gingiva (N = 25) and sites with severe chronic periodontitis (N = 25)...
October 29, 2018: Journal of Investigative and Clinical Dentistry
Bidur Bhandary, Qinghang Meng, Jeanne James, Hanna Osinska, James Gulick, Iñigo Valiente-Alandi, Michelle A Sargent, Md Shenuarin Bhuiyan, Burns C Blaxall, Jeffery D Molkentin, Jeffrey Robbins
Background Transforming growth factor beta ( TGF -β) is an important cytokine in mediating the cardiac fibrosis that often accompanies pathogenic cardiac remodeling. Cardiomyocyte-specific expression of a mutant αB-crystallin (Cry ABR 120G ), which causes human desmin-related cardiomyopathy, results in significant cardiac fibrosis. During onset of fibrosis, fibroblasts are activated to the so-called myofibroblast state and TGF -β binding mediates an essential signaling pathway underlying this process. Here, we test the hypothesis that fibroblast-based TGF -β signaling can result in significant cardiac fibrosis in a disease model of cardiac proteotoxicity that has an exclusive cardiomyocyte-based etiology...
October 16, 2018: Journal of the American Heart Association
Yanhua Lei, Ping Guo, Jun An, Chao Guo, Fengxiang Lu, Minglei Liu
BACKGROUND: Allergic rhinitis (AR) is the main cause of irreversible blindness in older individuals. Our study aims to identify the key genes and upstream regulators in AR. METHODS: To screen pathogenic genes of AR, an integrated analysis was performed by using the microarray datasets in AR derived from the Gene Expression Omnibus (GEO) database. The functional annotation and potential pathways of differentially expressed genes (DEGs) were further discovered by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis...
December 2018: International Journal of Pediatric Otorhinolaryngology
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