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Lymphatic endothelial cell

Gaetano Santulli
Lymphangiogenesis modulates inflammation by clearing immune cells in the infarcted heart in a lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1)-dependent process to ameliorate cardiac dysfunction.
July 25, 2018: Science Translational Medicine
Papa Diogop Ndiaye, Gilles Pagès
The lymphatic system is made up of vessels that drain interstitial fluids throughout the body. The circulation of the lymph (liquid in the lymphatic system) in the lymphatic vessels is unidirectional: tissues to the lymph nodes and then to the veins. Ganglia are mechanical filters but also immune barriers that can block the progression of certain pathogens as well as cancer cells. However, most studies on the lymphatic system and cancer highlight the role of the lymphatic network in metastatic dissemination as tumor cells use this network to reach other organs...
February 2019: Médecine Sciences: M/S
Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Miyuki Yanaka, Masato Sano, Hiroyuki Harada, Masato Fukui, Mika K Kaneko, Yukinari Kato
Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. One of the mAbs, PMab-219 (IgG2a , kappa), specifically detected CHO/horPDPN cells via flow cytometry and recognized horPDPN protein using Western blotting...
July 2019: Biochemistry and Biophysics Reports
Fatma Jendoubi, Manfred Rohde, Jörg Christoph Prinz
Erysipelas is a severe streptococcal infection of the skin primarily spreading through the lymphatic vessels. Penicillin is the treatment of choice. The most common complication consists in relapses which occur in up to 40% or more of patients despite appropriate antibiotic treatment. They cause lymphatic damage resulting in irreversible lymphedema and ultimately elephantiasis nostras and lead to major health restrictions and high socio-medical costs. Prevention of relapses is an unmet need, because even long-term prophylactic penicillin application does eventually not reduce the risk of recurrence...
2019: Frontiers in Medicine
Yinan Yuan, Valeria Arcucci, Sidney M Levy, Marc G Achen
The debilitating condition known as secondary lymphedema frequently occurs after lymphadenectomy and/or radiotherapy for the treatment of cancer. These therapies can damage lymphatic vessels leading to edema, fibrosis, inflammation and dysregulated adipogenesis, which result in profound swelling of an affected limb. Importantly, lymphedema patients often exhibit impaired immune function which predisposes them to a variety of infections. It is known that lymphadenectomy can compromise the acquisition of adaptive immune responses and antibody production; however the cellular mechanisms involved are poorly understood...
2019: Frontiers in Immunology
Yaeni Kim, Seun Deuk Hwang, Ji Hee Lim, Min Young Kim, Eun Nim Kim, Bum Soon Choi, Yong-Soo Kim, Hye Won Kim, Cheol Whee Park
Lymphangiogenesis occurs in response to renal injury and is correlated with interstitial fibrosis. Diabetes- and high-fat diet (HFD)-induced intrarenal lipotoxicity and their relationships with lymphangiogenesis are not established. We used PPARα agonist, fenofibrate, to unravel the linkage between lipotoxicity and lymphangiogenesis. Eight-week-old male C57BLKS/J db/db mice and HFD Spontaneously hypertensive rats (SHRs) were fed fenofibrate for 12 weeks. HK-2 and RAW264.7 cells were used to investigate their lymphangiogenic capacity in relation to lipotoxicity...
February 13, 2019: Scientific Reports
Erin D Lucas, Beth A J Tamburini
Lymphatic endothelial cells (LECs) form the structure of the lymphatic vessels and the sinuses of the lymph nodes, positioning them to be key players in many different aspects of the immune response. Following an inflammatory stimulus, LECs produce chemokines that recruit immune cells to the lymph nodes. The recruitment of immune cells aids in the coordination of both LEC and lymph node expansion and contraction. More recent data has demonstrated that to coordinate LEC division and death, cell surface molecules, such as PD-L1 and interferon receptors, are required...
2019: Frontiers in Immunology
Bo Sun, Yiming Zhou, Yantian Fang, Zhenyang Li, Xiaodong Gu, Jianbin Xiang
The lymphatic network remodeling may guide tumor metastasis in a sentinel lymph node (SLN). Although tumor-derived exosomes have been demonstrated to modify the microenvironment in adjacent organs and initiate a pre-metastatic niche, their influence on the lymphatic network in SLNs has not been explained. Here, we show that CT26 cell exosomes (Exo) promote the proliferation of lymphatic endothelial cells and the formation of lymphatic network in SLN, facilitating the SLN metastasis of colorectal cancer (CRC)...
February 8, 2019: International Journal of Cancer. Journal International du Cancer
Yoko Tsukita, Tatsuma Okazaki, Satoru Ebihara, Riyo Komatsu, Mayumi Nihei, Makoto Kobayashi, Taizou Hirano, Hisatoshi Sugiura, Tsutomu Tamada, Nobuyuki Tanaka, Yasufumi Sato, Hideo Yagita, Masakazu Ichinose
Tumor-associated blood vessels and lymphatics are abnormal and dysfunctional. These are hallmarks of the tumor microenvironment, which has an immunosuppressive nature, such as through hypoxia. Treatment with anti-death receptor5 (DR5) monoclonal antibody MD5-1, which induces tumor cell death, is a potent anti-tumor immunotherapy. Generally, MD5-1 induces cell death mainly via antigen presenting cells (APCs) and generates tumor-specific effector T cells. To date, the effects of a simultaneous functional improvement of abnormal blood vessels and lymphatics on the immune microenvironment are largely unknown...
2019: Oncoimmunology
Tara Karnezis, Rae Farnsworth, Nicole C Harris, Steven P Williams, Carol Caesar, David J Byrne, Prad Herle, Maria L Macheda, Ramin Shayan, You-Fang Zhang, Sezer Yazar, Simon J Takouridis, Craig Gerard, Stephen Fox, Marc G Achen, Steven A Stacker
The formation of new lymphatic vessels (lymphangiogenesis) and remodeling of existing lymphatics are thought to facilitate the entry and transport of tumor cells into lymphatic vessels and on to distant organs. The migration of lymphatic endothelial cells (LEC) toward guidance cues is critical for lymphangiogenesis. While chemokines are known to provide directional navigation for migrating immune cells, their role in mediating LEC migration during tumor-associated lymphangiogenesis is not well defined. Here we undertook gene profiling studies to identify chemokine-chemokine receptor pairs that are involved in tumor lymphangiogenesis associated with lymph node metastasis...
February 1, 2019: Cancer Research
Marius Otto, Sebastian Blatt, Andreas Pabst, Robert Mandic, Johanna Schwarz, Andreas Neff, Thomas Ziebart
OBJECTIVES: The aim of this murine in vivo study was to investigate whether buffy coat-derived putative endothelial progenitor cells (BCEPC ) alter tumor growth and neovascularization in oral squamous cell carcinomas (OSCC). MATERIALS AND METHODS: A murine xenograft model using the PCI-13 oral cancer cell line was deployed of which n = 24 animals received 2 × 106 BCEPC by transfusion whereas the control group (n = 24) received NaCl (0.9%) instead. Tumor size, volume, and capillary density were determined by sonography and measurement with a caliper...
January 28, 2019: Clinical Oral Investigations
Yiyu Sun, Bolun Lu, Jingcheng Deng, Zhaohua Jiang, Weigang Cao, Tingting Dai, Shengli Li
Our study was designed to investigate the effects of IL-7 during the differentiation process of adipose-derived stem cells (ADSCs) toward lymphatic endothelial cells (LECs). IL-7 was added to the traditional induced medium, which was called the IL-7 (+) group, while the group that used traditional induced medium was called the IL-7 (-) group. After 7 days of induction of ADSCs, a comprehensive analysis was conducted between these two groups. We examined the changes in Prox1, LYVE-1, Podoplanin and VEGFR-3 on the RNA and protein level and found that the expression of LEC markers in the IL-7 (+) group was higher than in the IL-7 (-) group...
January 28, 2019: Cell Biology International
Dongwon Choi, Eunkyung Park, Eunson Jung, Boksik Cha, Somin Lee, James Yu, Paul M Kim, Sunju Lee, Yeo Jin Hong, Chester J Koh, Chang-Won Cho, Yifan Wu, Noo Li Jeon, Alex K Wong, Laura Shin, S Ram Kumar, Ivan Bermejo-Moreno, R Sathish Srinivasan, Il-Taeg Cho, Young-Kwon Hong
The lymphatic system plays crucial roles in tissue homeostasis, lipid absorption and immune cell trafficking. While lymphatic valves ensure unidirectional lymph flows, the flow itself controls lymphatic valve formation. Here, we demonstrate that a mechanically activated ion channel Piezo1 senses oscillating shear stress (OSS), and incorporates the signal into the genetic program controlling lymphatic valve development and maintenance. Time-controlled deletion of Piezo1 using a pan-endothelial Cre driver (Cdh5(PAC)-CreERT2) or lymphatic-specific Cre driver (Prox1-CreERT2) equally inhibited lymphatic valve formation in newborn mouse...
January 24, 2019: JCI Insight
Jeffrey M Finlon, Matthew A Burchill, Beth A Jirón Tamburini
Within the liver, lymphatic vessels are found within the portal triad, and their described function is to remove interstitial fluid from the liver to the lymph nodes where cellular debris and antigens can be surveyed. We are very interested in understanding how the lymphatic vasculature might be involved in inflammation and immune cell function within the liver. However, very little has been published establishing digestion protocols for the isolation of lymphatic endothelial cells (LECs) from the liver or specific markers that can be used to evaluate liver LECs on a per cell basis...
January 7, 2019: Journal of Visualized Experiments: JoVE
Nicolae Ghinea, Blaise Robin, Christophe Pichon, Renaud Leclere, André Nicolas, Caroline Chnecker, Jean-François Côté, Bertrand Guillonneau, Aurelian Radu
BACKGROUND: Perineural invasion (PNI) is generally accepted as a major route of cancer dissemination in malignancies associated with highly enervated organs. However, the effect of cancer cells on vasa nervorum remains unknown. We studied this effect in locally advanced prostate cancer, a high-risk feature associated with approximately 20% of prostate cancer specific mortality. METHODS: We used immunohistochemistry for CD34, fibroblast growth factor-2 (FGF-2), FSHR, podoplanin, vascular endothelial growth factor (VEGF), and VEGFR-2 as well as histochemical methods to examine the vasa nervorum of nerves invaded by cancer cells in tissue samples from 85 patients...
January 20, 2019: Prostate
Kyuho Jeong, James M Murphy, Yelitza A R Rodriguez, Jun-Sub Kim, Eun-Young Erin Ahn, Ssang-Taek Steve Lim
Malignant melanoma typically metastasizes to lymph nodes (LNs) as a primary or in-transit lesion before secondary metastasis occurs, and LN biopsy is a common procedure to diagnose melanoma progression. Since cancer metastasis is a complex process where various interactions between tumor cells and the stroma play key roles in establishing metastatic lesions, the exact mechanisms underlying melanoma metastasis to LNs remains unknown. It has been known that focal adhesion kinase (FAK) activity promotes the expression of proinflammatory vascular cell adhesion molecule-1 (VCAM-1)...
January 16, 2019: Biochemical and Biophysical Research Communications
David P Perrault, Gene K Lee, Sun Young Park, Sunju Lee, Dongwon Choi, Eunson Jung, Young Jin Seong, Eun Kyung Park, Cynthia Sung, Roy Yu, Antoun Bouz, Austin Pourmoussa, Soo Jung Kim, Young-Kwon Hong, Alex K Wong
BACKGROUND: The fibroblast growth factor receptor (FGFR) family includes transmembrane receptors involved in a wide range of developmental and postdevelopmental biologic processes as well as a wide range of human diseases. In particular, FGFR3 has been implicated in the mechanism by which 9-cis retinoic acid (9-cisRA) induces lymphangiogenesis and improves lymphedema. The purpose of this study was to validate the efficacy of a novel small peptide FGFR3 inhibitor, peptide P3 (VSPPLTLGQLLS), and to elucidate the role of FGFR3 in 9-cisRA-induced lymphangiogenesis using this peptide...
January 16, 2019: Lymphatic Research and Biology
Jia Wang, Yanhong Ma, Jingshi Yang, Lu Jin, Zixiang Gao, Lingyun Xue, Lin Hou, Linlin Sui, Jing Liu, Xiangyang Zou
Tumour lymphangiogenesis plays an important role in promoting the growth and lymphatic metastasis of tumours. The process is associated with cell proliferation, migration and tube-like structure formation in lymphatic endothelial cells (LEC), but no antilymphangiogenic agent is currently used in clinical practice. Fucoxanthin is a material found in brown algae that holds promise in the context of drug development. Fucoxanthin is a carotenoid with variety of pharmacological functions, including antitumour and anti-inflammatory effects...
January 16, 2019: Journal of Cellular and Molecular Medicine
Yan-Li Chen, Yu Jiang, Tian-Wu Chen, Rui Li, Xiao-Ming Zhang, Fan Chen, Lan Wu, Jing Ou, Jian-Qiong Yang
This study aimed to determine whether dynamic contrast-enhanced MRI (DCE-MRI) derived parameters can identify oesophageal squamous cell carcinoma (SCC) and lymphatic metastasis. Thirty-nine oesophageal SCC patients underwent DCE-MRI. Quantitative parameters including endothelial transfer constant (Ktrans ), reflux rate (Kep ), fractional extravascular extracellular space volume and fractional plasma volume, and semi-quantitative parameters including time to peak (TTP), max concentration, Max Slope and area under concentration-time curve of both oesophageal SCC and normal oesophagus were measured...
January 15, 2019: Scientific Reports
Enxue Liu, Xuejing Sun, Xindong Wang, Taozhi Wang, Wenqian Li, Imran Tarique, Ping Yang, Qiusheng Chen
Exosomes are vesicles secreted by the multivesicular bodies (MVBs), which have been shown to mediate immunity regulation and virus transmission. In this study, the dynamic distribution and function of the MVBs and their exosomes was investigated through morphological characterization and molecular analyses in duck spleens infected with duck Tembusu virus (DTMUV) at different times post infection (1hpi, 2hpi, 12hpi, 24hpi). CD63, the marker of MVBs and exosomes, was distributed in the sheathed capillaries and the periellipsoidal lymphatic sheaths (PELS) of the white pulp...
February 2019: Veterinary Microbiology
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