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Mélanie J Domingues, Juan Martinez-Sanz, Laura Papon, Lionel Larue, Liliane Mouawad, Jacky Bonaventure
ICAT (Inhibitor of β-CAtenin and TCF) is a small acidic protein that negatively regulates β-catenin co-transcriptional activity by competing with TCF/LEF factors in their binding to β-catenin superhelical core. In melanoma cells, ICAT competes with LEF1 to negatively regulate the M-MITF and NEDD9 target genes. The structure of ICAT consists of two domains: the 3-helix bundle N-terminal domain binds to β-catenin Armadillo (Arm) repeats 10-12 and the C-terminal tail binds to Arm repeats 5-9. To elucidate the structural mechanisms governing ICAT/β-catenin interactions in melanoma cells, three ICAT residues Y15, K19 and V22 in the N-terminal domain, contacting hydrophobic β-catenin residue F660, were mutated and interaction was assessed by immunoprecipitation...
2017: PloS One
Maarten Gees, Yeranddy A Alpizar, Brett Boonen, Alicia Sanchez, Wouter Everaerts, Andrei Segal, Fenqin Xue, Annelies Janssens, Grzegorz Owsianik, Bernd Nilius, Thomas Voets, Karel Talavera
Allyl isothiocyanate (AITC; aka, mustard oil) is a powerful irritant produced by Brassica plants as a defensive trait against herbivores and confers pungency to mustard and wasabi. AITC is widely used experimentally as an inducer of acute pain and neurogenic inflammation, which are largely mediated by the activation of nociceptive cation channels transient receptor potential ankyrin 1 and transient receptor potential vanilloid 1 (TRPV1). Although it is generally accepted that electrophilic agents activate these channels through covalent modification of cytosolic cysteine residues, the mechanism underlying TRPV1 activation by AITC remains unknown...
September 2013: Molecular Pharmacology
F I Kuzminov, N V Karapetyan, M G Rakhimberdieva, I V Elanskaya, M Y Gorbunov, V V Fadeev
In order to prevent photodestruction by high light, photosynthetic organisms have evolved a number of mechanisms, known as non-photochemical quenching (NPQ), that deactivate the excited states of light harvesting pigments. Here we investigate the NPQ mechanism in the cyanobacterium Synechocystis sp. PCC 6803 mutant deficient in both photosystems. Using non-linear laser fluorimetry, we have determined molecular photophysical characteristics of phycocyanin and spectrally distinct forms of allophycocyanin for the cells in non-quenched and quenched states...
July 2012: Biochimica et Biophysica Acta
Eduardo Aneiros, Lishuang Cao, Marianthi Papakosta, Edward B Stevens, Stephen Phillips, Christian Grimm
The capsaicin receptor TRPV1, a member of the transient receptor potential family of non-selective cation channels is a polymodal nociceptor. Noxious thermal stimuli, protons, and the alkaloid irritant capsaicin open the channel. The mechanisms of heat and capsaicin activation have been linked to voltage-dependent gating in TRPV1. However, until now it was unclear whether proton activation or potentiation or both are linked to a similar voltage-dependent mechanism and which molecular determinants underlie the proton gating...
March 16, 2011: EMBO Journal
S M Wu, C A Rebeiz
Nuclear magnetic resonance analysis established the presence of two vinyl groups/macrocycle of chlorophyll b (E489 F666). The latter is the only chlorophyll b that accumulates in a corn mutant (ON 8147). On the other hand the presence of only one vinyl group/macrocycle of authentic monovinyl chlorophyll b (E475 F660) was confirmed.
March 25, 1985: Journal of Biological Chemistry
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