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Aminoglycoside resistance

Yaru Hu, Tianyang Zhang, Lei Jiang, Yi Luo, Shijie Yao, Dong Zhang, Kuangfei Lin, Changzheng Cui
Antibiotic resistance is extensively detected in drinking water sources, threatening its safety and human health, which deserves further attention to the removal of antibiotic resistance genes (ARGs) in the drinking water system. In this study, the occurrence and reduction of integrase gene intI1 and forty-one ARG subtypes, which confers resistance to six antibiotic classes (β-lactam, aminoglycoside, macrolide, tetracycline, sulfonamide, and quinolone), were investigated in a drinking water treatment plant (DWTP)...
March 11, 2019: Science of the Total Environment
Mariana Castanheira, Lalitagauri M Deshpande, Rodrigo E Mendes, Rafael Canton, Helio S Sader, Ronald N Jones
Background: A total of 178 825 Enterobacteriaceae isolates collected in 199 hospitals from 42 countries worldwide over 20 years (1997 to 2016) of the SENTRY Program were susceptibility tested by reference broth microdilution methods. Methods: Trends in percentages over time were analyzed by the χ2 test. Results were reported as the percentage difference between the first (1997-2000) and the last (2013-2016) time period. Results: Enterobacteriaceae exhibiting resistance to cephalosporins (extended-spectrum β-lactamase [ESBL] phenotype) and carbapenem resistance (CRE) significantly increased ( P < 0...
March 2019: Open Forum Infectious Diseases
Derek R MacFadden, Roberto G Melano, Bryan Coburn, Nathalie Tijet, William P Hanage, Nick Daneman
Rapid diagnostic tests of antibiotic resistance are increasingly being developed that identify the presence or absence of antibiotic resistance genes/loci. However, these approaches usually neglect other sources of predictive information, which could be identified in shorter time periods, including patient epidemiologic risk factors for antibiotic resistance and markers of lineage. Using a dataset of 414 Escherichia coli isolated from separate episodes of bacteremia at a single academic institution in Toronto, Canada between 2010-2015, we compared the potential predictive ability of three approaches (epidemiologic, pathogen sequence type, resistance gene identification) for classifying phenotypic antibiotic resistance to three antibiotics representing classes of broad spectrum antimicrobial therapy (ceftriaxone - 3rd generation cephalosporins, ciprofloxacin - fluoroquinolones, and gentamicin - aminoglycosides)...
March 20, 2019: Journal of Clinical Microbiology
Bradd J Haley, Seon Woo Kim, Julie Haendiges, Eric Keller, David Torpey, Alexander Kim, Kia Crocker, Robert A Myers, Jo Ann S Van Kessel
Salmonella Kentucky is among the most frequently isolated S. enterica serovars from food animals in the United States. Recent research on isolates recovered from these animals suggests there may be geographic and host specificity signatures associated with S. Kentucky strains. However, the sources and genomic features of human clinical S. Kentucky isolated in the United States remain poorly described. To investigate the characteristics of clinical S. Kentucky and the possible sources of these infections, the genomes of all S...
March 19, 2019: Zoonoses and Public Health
Arif Istiaq, Md Sadikur Rahman Shuvo, Khondaker Md Jaminur Rahman, Mohammad Anwar Siddique, M Anwar Hossain, Munawar Sultana
Chromosomal co-existence of metal and antibiotic resistance genes in bacteria offers a new perspective to the bacterial resistance proliferation in contaminated environment. In this study, an arsenotrophic bacterium Achromobacter xylosoxidans BHW-15, isolated from Arsenic (As) contaminated tubewell water in the Bogra district of Bangladesh, was analyzed using high throughput Ion Torrent Personal Genome Machine (PGM) complete genome sequencing scheme to reveal its adaptive potentiality. The assembled draft genome of A...
2019: PeerJ
Jean Ferreira Machado, Maria do Socorro Costa, Saulo Relison Tintino, Fábio Fernandes Galvão Rodrigues, Camila Bezerra Nobre, Henrique Douglas Melo Coutinho, José Galberto Martins da Costa, Irwin Rose Alencar de Menezes, Erlânio Oliveira de Sousa
Orbignya speciosa (babassu) is an important palm tree in Brazil whose fixed almond oil is used in popular medicine and especially in food, in addition to being a research target for the manufacture of biofuels. The aim of this study was to evaluate the fixed almond oil physicochemical characterization and its antibacterial activity in isolation and in association with aminoglycosides against standard and multidrug-resistant bacteria. Analyses such as water content, pH, acidity, peroxide index, relative density, and refractive index indicate the stability and chemical quality of the oil...
March 17, 2019: Antibiotics
João Pedro Rueda Furlan, Quézia Moura, Irys Hany Lima Gonzalez, Patrícia Locosque Ramos, Nilton Lincopan, Eliana Guedes Stehling
OBJECTIVES: This study aimed to report the draft genome sequence of a multidrug-resistant (MDR) Escherichia coli colonizing a giant anteater (Myrmecophaga tridactyla) in a Brazilian Zoo. METHODS: The genome was sequenced using the Illumina MiSeq Platform and de novo genome assembly was performed using SPAdes v. 3.9. The draft genome sequence was annotated using NCBI Prokaryotic Genome Annotation Pipeline. Antibiotic resistance genes, virulence genes, sequence type, serotype and plasmid incompatibility groups were identified using tools from the Center for Genomic Epidemiology...
March 12, 2019: Journal of Global Antimicrobial Resistance
Ana Beatriz Linard de Carvalho, Cleciana Alves Cruz, Cicero Lucas Almeida de Freitas, José Junior Dos Santos Aguiar, Paula Leticia Wendy de Souza Nunes, Valéria Maria da Silva Lima, Edinardo Fagner Ferreira Matias, Débora Feitosa Muniz, Henrique Douglas Melo Coutinho
The present study aimed to determine the chemical profile and to evaluate the antibacterial activity and antibiotic-modulating action of the hexanic Zea mays silk extract in association with aminoglycosides. Standard Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 strains, as well as multi-resistant Escherichia coli 27, Staphylococcus aureus 35, and Pseudomonas aeruginosa 31 strains, were used in this study. Phytochemical prospection revealed the presence of the following secondary metabolites: tannins, flavones, flavonoids, and xanthones, with the main chemical constituents being identified in plant extracts obtained with apolar organic solvents such as hexane...
March 12, 2019: Antibiotics
Andrew J Nunn, Patrick P J Phillips, Sarah K Meredith, Chen-Yuan Chiang, Francesca Conradie, Doljinsuren Dalai, Armand van Deun, Phan-Thuong Dat, Ngoc Lan, Iqbal Master, Tesfamarium Mebrahtu, Daniel Meressa, Ronelle Moodliar, Nosipho Ngubane, Karen Sanders, Stephen Bertel Squire, Gabriela Torrea, Bazarragchaa Tsogt, I D Rusen
BACKGROUND: Cohort studies in Bangladesh showed promising cure rates among patients with multidrug-resistant tuberculosis who received existing drugs in regimens shorter than that recommended by the World Health Organization (WHO) in 2011. METHODS: We conducted a phase 3 noninferiority trial in participants with rifampin-resistant tuberculosis that was susceptible to fluoroquinolones and aminoglycosides. Participants were randomly assigned, in a 2:1 ratio, to receive a short regimen (9 to 11 months) that included high-dose moxifloxacin or a long regimen (20 months) that followed the 2011 WHO guidelines...
March 13, 2019: New England Journal of Medicine
Rakesh Yadav, Aastha Saini, Jureka Mankotia, Rajiv Khaneja, Priyanka Agarwal, Sunil Sethi
Rapid detection of drug resistance in Mycobacterium tuberculosis is important for the successful treatment of tuberculosis. Fluoroquinolone and aminoglycoside resistance detection by molecular methods becomes more complex due to cross resistance among them. Thus, we aimed to determine cross-resistance and mutations in resistance genes for these drugs. A total of 336 multidrug-resistant tuberculosis (MDR-TB) cases received in Mycobacteriology laboratory were screened for phenotypic drug sensitivity testing for second-line drugs, i...
December 2018: Journal of Epidemiology and Global Health
Rachel F Eyler, Kristina Shvets
Antimicrobial pharmacology and its effect on prescribing is quite complex. Selecting an antibiotic that will optimally treat an infection while minimizing adverse effects and the development of resistance is only the first step, as one must also consider the patient's individual pharmacokinetic alterations and the pharmacodynamic properties of the drug when prescribing it as well. Patients with CKD may have alterations in their protein binding, volumes of distribution, kidney clearance, and nonrenal clearance that necessitates antibiotic dose adjustments to prevent the development of toxicity...
March 12, 2019: Clinical Journal of the American Society of Nephrology: CJASN
Zeyou Chen, Wei Zhang, Luxi Yang, Robert D Stedtfeld, Anping Peng, Cheng Gu, Stephen A Boyd, Hui Li
Land application of animal manure could change the profiles of antibiotic resistant bacteria (ARB), antibiotic resistance genes (ARGs) and bacterial communities in receiving soils. Using high-throughput real-time quantitative PCR and 16S rRNA amplicon sequencing techniques, this study investigated the ARGs and bacterial communities in field soils under various crop (corn and pasture) and manure (swine and dairy) managements, which were compared with those of two non-manured reference soils from adjacent golf course and grassland...
March 1, 2019: Environmental Pollution
Fernando Sanz-García, Carolina Alvarez-Ortega, Jorge Olivares-Pacheco, Paula Blanco, José Luis Martínez, Sara Hernando-Amado
High-throughput screening of transposon insertion libraries is a useful strategy for unveiling bacterial genes whose inactivation results in an altered susceptibility to antibiotics. A potential drawback of these studies is they are usually based on just one model antibiotic for each structural family, under the assumption that the results can be extrapolated to all members of said family. To determine if this simplification is appropriate, we have analysed the susceptibility of mutants of P. aeruginosa to four aminoglycosides...
March 11, 2019: Antimicrobial Agents and Chemotherapy
Jacinda C Abdul-Mutakabbir, Razieh Kebriaei, Sarah C J Jorgensen, Michael J Rybak
The emergence of multi-drug resistant (MDR) Gram-negative pathogens has become a serious worldwide health concern. Gram-negative bacteria such as Enterobacteriaceae (Klebsiella pneumoniae, Escherichia coli, Enterobacter spp.,) Acinetobacter spp., and Pseudomonas aeruginosa have rendered most antibiotics inactive, leaving aminoglycosides and polymyxins. Plazomicin (formerly ACHN-490), is a neoglycoside with unique structural modifications to the aminoglycoside pharmacophore that impart activity against many MDR Gram-negative organisms...
March 9, 2019: Infectious Diseases and Therapy
Alexander Lim, Bryan Naidenov, Haley Bates, Karyn Willyerd, Timothy Snider, Matthew Brian Couger, Charles Chen, Akhilesh Ramachandran
Disruptive innovations in long-range, cost-effective direct template nucleic acid sequencing are transforming clinical and diagnostic medicine. A multidrug resistant strain and a pan-susceptible strain of Mannheimia haemolytica, isolated from pneumonic bovine lung samples, were sequenced at 146× and 111× coverage, respectively with Oxford Nanopore Technologies MinION. De novo assembly produced a complete genome for the non-resistant strain and a nearly complete assembly for the drug resistant strain. Functional annotation using RAST (Rapid Annotations using Subsystems Technology), CARD (Comprehensive Antibiotic Resistance Database) and ResFinder databases identified genes conferring resistance to different classes of antibiotics including β-lactams, tetracyclines, lincosamides, phenicols, aminoglycosides, sulfonamides and macrolides...
March 5, 2019: Journal of Microbiological Methods
Irene Galani, Konstantina Nafplioti, Panagiota Adamou, Ilias Karaiskos, Helen Giamarellou, Maria Souli
Following publication of the original article [1].
March 7, 2019: BMC Infectious Diseases
Song Zhe, Zhao Qianru, Zhu Liying, Zhang Zhidong, Jiang Ling, Huang He
OBJECTIVES: Bacillus cereus is associated with a variety of diseases in the clinic and may cause human diseases such as hematological malignancies, tetanus, gas gangrene, food poisoning and pseudomembranous colitis. Our team found and isolated a strain called B. cereus S66 from radiation-contaminated soil in Xinjiang Uygur Autonomous Region. Here, we systematically studied and analyzed its drug resistance and toxicity. METHODS: Genomic DNA ofB. cereus S66 was sequenced using the Illumina HiSeq 2000...
March 4, 2019: Journal of Global Antimicrobial Resistance
Lebogang Ramma, Primrose T Nhokwara, Christine Rogers
BACKGROUND:  South Africa is a high-burden multi-drug-resistant tuberculosis (MDR-TB) country. Previously, standard MDR-TB treatment regimen in South Africa included kanamycin, an aminoglycoside, which can cause permanent hearing loss in patients. South African National Tuberculosis Control programme's guidelines for the management of patients with MDR-TB were revised in 2011 to support outpatient-based models. This, in turn, required reorganisation of ototoxicity monitoring services to support these new models of service delivery...
January 30, 2019: South African Journal of Communication Disorders. die Suid-Afrikaanse Tydskrif Vir Kommunikasieafwykings
Patrick K Taylor, Li Zhang, Thien-Fah Mah
The two-component system TctD-TctE is important for regulating the uptake of tricarboxylic acids in Pseudomonas aeruginosa TctD-TctE accomplishes this through derepression of the gene opdH , which encodes a tricarboxylic acid-specific porin. Previous work from our lab revealed that TctD-TctE in P. aeruginosa also has a role in resistance to aminoglycoside antibiotics. The aim of this study was to further characterize the role of TctD-TctE in P. aeruginosa in the presence of citric acid. Here it was found that deletion of P...
March 6, 2019: MSphere
M Brilhante, V Perreten, V Donà
Enterovirulent Escherichia coli infections cause significant losses in the pig industry. However, information about the structures of the virulence and multidrug resistance (MDR) plasmids harboured by these strains is sparse. In this study, we used whole-genome sequencing with PacBio and Illumina platforms to analyse the molecular features of the multidrug-resistant enterotoxigenic E. coli (ETEC) strain 14OD0056 and the multidrug-resistant hybrid Shiga toxin-producing/enterotoxigenic E. coli (STEC/ETEC) strain 15OD0495 isolated from diarrheic pigs in Switzerland...
February 2019: Veterinary Journal
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