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Fertilization in vitro

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https://read.qxmd.com/read/30762725/effect-of-hysteroscopy-before-starting-in-vitro-fertilization-for-women-with-recurrent-implantation-failure-a-meta-analysis-and-systematic-review
#1
Xiaoyan Mao, Ling Wu, Qiuju Chen, Yanping Kuang, Shaozhen Zhang
OBJECTIVE: To study if hysteroscopy (HSC) before starting an in-vitro fertilization (IVF) cycle improves IVF outcomes in women with recurrent implantation failure (RIF). METHODS: The Medline, Cochrane, EMBASE, and Google Scholar databases were searched using the following keywords until March 31, 2017: in-vitro fertilization; infertility; hysteroscopy; recurrence; embryo implantation; and pregnancy. Randomized controlled trials (RCTs), two-arm prospective studies, and retrospective studies were included...
February 2019: Medicine (Baltimore)
https://read.qxmd.com/read/30761454/expanded-genetic-carrier-screening-in-clinical-practice-a-current-survey-of-patient-impressions-and-attitudes
#2
Nigel Pereira, Michelle Wood, Emerly Luong, Allison Briggs, Michael Galloway, Rose A Maxwell, Steven R Lindheim
PURPOSE: Expanded genetic carrier screening (ECS) is an important part of gynecological practice and preconception planning. We evaluated the awareness and attitudes among women regarding ECS and factors that may influence decision-making in a family planning context. METHODS: A 32-question survey in an academic university practice was given to 521 women who were either currently pregnant (n = 108), undergoing gynecologic care who were considering future fertility (n = 308), and considering or receiving fertility treatment (n = 105)...
February 13, 2019: Journal of Assisted Reproduction and Genetics
https://read.qxmd.com/read/30760649/effects-of-all-trans-retinoic-acid-on-the-in-vitro-maturation-of-camel-camelus-dromedarius-cumulus-oocyte-complexes
#3
Islam M Saadeldin, Ayman Abdel-Aziz Swelum, Mona Elsafadi, Amer Mahmood, Syed Hilal Yaqoob, Musaad Alfayez, Abdullah N Alowaimer
All-trans retinoic acid (RA) is a metabolite of vitamin A and has pleiotropic actions on many different biological processes, including cell growth and differentiation, and is involved in different aspects of fertility and developmental biology. In the current study, we investigated the effects of RA on camel (Camelus dromedarius) cumulus-oocyte complex in vitro maturation (IVM). IVM medium was supplemented with 0, 10, 20, and 40 µM RA. Application of 20 µM RA significantly reduced the proportion of degenerated oocytes and significantly improved oocyte meiosis and first polar body extrusion compared to the control and other experimental groups...
February 14, 2019: Journal of Reproduction and Development
https://read.qxmd.com/read/30760574/genome-characterization-prevalence-and-transmission-mode-of-a-novel-picornavirus-associated-with-the-threespine-stickleback-fish-gasterosteus-aculeatus
#4
Megan A Hahn, Nolwenn M Dheilly
The complete genome sequence of an RNA virus was assembled from RNA sequencing of virus particles purified from threespine stickleback intestine tissue samples. This new virus is most closely related to the Eel Picornavirus and can be assigned to the genus Potamipivirus in the family Picornaviridae Its unique genetic properties are enough to establish a new species, dubbed the Threespine Stickleback Picornavirus (TSPV). Due to their broad geographic distribution throughout the northern hemisphere and parallel adaptation to freshwater, threespine sticklebacks have become a model in evolutionary ecology...
February 13, 2019: Journal of Virology
https://read.qxmd.com/read/30753900/-assisted-reproductive-technologies-in-hiv-patients-which-results-and-which-strategy-in-france-in-2019
#5
Clémentine Ninive, Xavier Ferrareto, Solenne Gricourt, Marie-Astrid Llabadorde Royer, Julien Lepage, Claire Gauché-Cazalis, Sylvie Epelboin, Maëliss Peigné
With the use of antiretroviral therapy, HIV transmission from mother to fetus and between sexual partners has been significantly reduced, successively allowing pregnancy, then assisted reproductive technologies, and targeted unprotected sex among couples living with HIV. Since first french Morlat report in 2013, natural procreation is now possible under certain conditions for these couples living with HIV and the use of assisted reproductive technologies is increasingly limited to the treatment of infertility...
February 9, 2019: Gynecologie, Obstetrique, Fertilite & Senologie
https://read.qxmd.com/read/30745523/improvement-of-fertility-in-repeat-breeder-dairy-cattle-by-embryo-transfer-following-artificial-insemination-possibility-of-interferon-tau-replenishment-effect
#6
Hikari Yaginuma, Natsumi Funeshima, Nao Tanikawa, Motoharu Miyamura, Hideki Tsuchiya, Tatsuo Noguchi, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna, Seizo Hamano
Repeat breeder cattle do not become pregnant until after three or more breeding attempts; this represents a critical reproductive disorder. Embryo transfer (ET) following artificial insemination (AI) in repeat breeder cattle reportedly improves pregnancy rate, leading to speculation that interferon tau (IFNT) is associated with this phenomenon. However, the reason why the conception rate improves remains unknown. We investigated the effect of ET following AI on repeat breeder cattle in field tests, and determined whether adding an embryo affects the maternal immune cells detected by interferon-stimulated genes (ISGs), marker genes of IFN response...
February 8, 2019: Journal of Reproduction and Development
https://read.qxmd.com/read/30745236/assessment-of-the-calcium-releasing-machinery-in-oocytes-that-failed-to-fertilize-after-conventional-icsi-and-assisted-oocyte-activation
#7
Minerva Ferrer-Buitrago, Davina Bonte, Lien Dhaenens, Sanne Vermorgen, Yuechao Lu, Petra De Sutter, Björn Heindryckx
RESEARCH QUESTION: Can oocyte-related activation deficiencies be evaluated in oocytes that failed to fertilize after intracytoplasmic sperm injection (ICSI) combined with assisted oocyte activation (AOA)? DESIGN: Evaluation of the spindle-chromosome complexes and intracellular distribution of inositol trisphosphate type 1 receptors (IP3R1) in in-vitro matured (IVM) and failed-to-fertilize oocytes from patients undergoing AOA. Assessment of the oocyte-related Ca2+ releasing capacity in response to Ca2+ ionophores and sperm microinjection in oocytes that failed to fertilize after ICSI or ICSI-AOA...
December 23, 2018: Reproductive Biomedicine Online
https://read.qxmd.com/read/30743159/impact-of-in-vitro-fertilization-preimplantation-genetic-testing-ivf-pgt-funding-policy-on-clinical-outcome-an-issue-that-stems-beyond-effectiveness-of-treatment
#8
Yi-Hong Shao, Xiao Yun Zhang, William Buckett, Asangla Ao
OBJECTIVE(S): The aim of this study was to compare the patient characteristics, type of genetic disease and inheritance, volume of activity, practice patterns and pregnancy outcomes, in private versus publically funded IVF pre-implantation genetic testing (PGT) for translocation (IVF-PGT-SR) and aneuploidy (PGT-A) periods. STUDY DESIGN: This study retrospectively analyzed data during both privately funded period (PRP) and publically funded period (PUP) of assisted reproductive technology (ART) for a total of 275 patients...
February 8, 2019: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://read.qxmd.com/read/30742944/knock-down-of-oncohistone-h3f3a-g34w-counteracts-the-neoplastic-phenotype-of-giant-cell-tumor-of-bone-derived-stromal-cells
#9
J Fellenberg, H Sähr, D Mancarella, C Plass, A M Lindroth, F Westhauser, B Lehner, V Ewerbeck
Giant cell tumors of bone (GCTB) are semi-malignant tumors associated with extensive osteolytic defects and massive bone destructions. They display a locally aggressive behavior and a very high recurrence rate. Recently, a single mutation has been identified in GCTB affecting the H3F3A gene coding for the histone variant H3.3 (H3.3-G34W). The aim of this study was to investigate whether H3.3-G34W is sufficient to drive tumorigenesis in GCTB. Initially, we confirmed the high frequency of this mutation in 94 % of 84 analyzed tissue samples...
February 8, 2019: Cancer Letters
https://read.qxmd.com/read/30740547/autonomy-in-altruistic-surrogacy-conflicting-kinship-grammars-and-intentional-multilineal-kinship
#10
Jenny Gunnarsson Payne
Since 1982, when the first baby conceived by in-vitro fertilization (IVF) in Sweden was born, Swedish legislation on assisted reproduction has gradually become more liberal and inclusive. Today, gamete donation and IVF are permitted not only for heterosexual couples, but also for lesbian couples and single women, and embryo donation is expected to become legalized shortly which will further increase the chances for involuntarily childless people to become parents. In recent years, the possibility of allowing surrogacy has been debated increasingly, with strongly polarized arguments both for and against it...
November 2018: Reproductive Biomedicine & Society Online
https://read.qxmd.com/read/30740498/pseudoangiomatous-stromal-hyperplasia-presenting-as-an-axillary-mass-in-a-postmenopausal-woman-undergoing-in-vitro-fertilization
#11
Jesse Liu, Megan Casady, Kristen Paral, Jennifer Powers
No abstract text is available yet for this article.
February 2019: JAAD Case Reports
https://read.qxmd.com/read/30740489/data-on-knowledge-and-beliefs-of-students-physicians-and-other-health-professionals-about-ethical-issues-in-cyprus
#12
Rafaella Christou, Niki Michael, Eirini Stylianou, Anneza I Yiallourou, Katerina Pantavou, Georgios K Nikolopoulos
This article presents data collected in a field, questionnaire-based survey about ethical issues in the Republic of Cyprus. The participants were students of the University of Cyprus, and physicians and other health professionals of the Medical School, University of Cyprus and of the Archbishop Makarios III Hospital. The questionnaire included items on sociodemographic characteristics of the participants, and on their knowledge and beliefs about three different ethical issues. Beliefs on the same ethical issues but under specific, hypothetical scenarios were also reported by the participants...
February 2019: Data in Brief
https://read.qxmd.com/read/30737682/culture-and-host-transfer-of-xenopus-oocytes-for-maternal-mrna-depletion-and-genome-editing-experiments
#13
Douglas W Houston
The early development of Xenopus critically depends on maternal components stored in the egg. Because important events such as axis formation are triggered immediately after fertilization, it is often desirable to perturb gene function before this occurs. Oocytes can be experimentally manipulated in vitro, prior to maturation, and subsequently fertilized or otherwise activated to develop, and then observed for any embryological defects. Available methods for fertilizing cultured oocytes include in vitro fertilization following oocyte vitelline envelope removal, nuclear transplantation, intracytoplasmic sperm injection, and transferring oocytes to the body cavity of ovulating host females (host transfer)...
2019: Methods in Molecular Biology
https://read.qxmd.com/read/30737004/in-vitro-fertilization-and-infertility-do-not-cause-a-significant-alteration-in-placental-gene-expression-at-the-end-of-the-first-trimester
#14
EDITORIAL
Diego Marin, Emre Seli
No abstract text is available yet for this article.
February 5, 2019: Fertility and Sterility
https://read.qxmd.com/read/30737003/30-years-of-data-impact-of-the-united-states-in-vitro-fertilization-data-registry-on-advancing-fertility-care
#15
Tarun Jain, David A Grainger, G David Ball, William E Gibbons, Robert W Rebar, Jared C Robins, Richard E Leach
OBJECTIVE: To summarize and assess the impact of key research generated through the Society of Assisted Reproductive Technology (SART)-initiated United States IVF registry and annual reporting system. DESIGN: Review. SETTING: Eligible studies included those that analyzed data generated by the National IVF data collection program (through SART or Centers for Disease Control and Prevention). PATIENT(S): Not applicable...
February 5, 2019: Fertility and Sterility
https://read.qxmd.com/read/30734403/enu-induced-mutant-allele-of-dnah1-ferf1-causes-abnormal-sperm-behavior-and-fertilization-failure-in-mice
#16
Jianjun Hu, Carl Lessard, Charles Longstaff, Marilyn O'Brien, Kristina Palmer, Laura Reinholdt, John Eppig, John Schimenti, Mary Ann Handel
Given attention to both contraception and treatment of infertility, there is a need to identify genes and sequence variants required for mammalian fertility. Recent unbiased mutagenesis strategies have expanded horizons of genetic control of reproduction. Here we show that male mice homozygous for the ethyl-nitroso-urea-induced ferf1 (fertilization failure 1) mutation are infertile, producing apparently normal sperm that does not fertilize oocytes in standard fertilization in vitro fertilization assays. The ferf1 mutation is a single-base change in the Dnah1 gene, encoding an axoneme-associated dynein heavy chain, and previously associated with male infertility in both mice and humans...
February 8, 2019: Molecular Reproduction and Development
https://read.qxmd.com/read/30733926/vaginal-culture-for-ivf-allows-two-mothers-to-carry-the-same-pregnancy-is-more-always-better
#17
EDITORIAL
Sara Babcock Gilbert, Alex J Polotsky
•Intravaginal culture (IVC) is not a new technology but is gaining in popularity•The INVOcell device is marketed as a lower cost treatment option to in-vitro fertilization (IVF)•Previous studies done by the INVOcell owned company have shown similar live birth rates between INVOcell IVC and IVF•It is unclear how much of a cost savings is present with use of IVC compared to traditional IVF•IVC may represent a viable option for fertility treatment in select patients.
January 2019: Case Reports in Women's Health
https://read.qxmd.com/read/30732658/association-of-four-imprinting-disorders-and-art
#18
Hiromitsu Hattori, Hitoshi Hiura, Akane Kitamura, Naoko Miyauchi, Norio Kobayashi, Souta Takahashi, Hiroaki Okae, Koichi Kyono, Masayo Kagami, Tsutomu Ogata, Takahiro Arima
BACKGROUND: Human-assisted reproductive technologies (ART) are a widely accepted treatment for infertile couples. At the same time, many studies have suggested the correlation between ART and increased incidences of normally rare imprinting disorders such as Beckwith-Wiedemann syndrome (BWS), Angelman syndrome (AS), Prader-Willi syndrome (PWS), and Silver-Russell syndrome (SRS). Major methylation dynamics take place during cell development and the preimplantation stages of embryonic development...
February 7, 2019: Clinical Epigenetics
https://read.qxmd.com/read/30725347/a-report-on-three-live-births-in-women-with-poor-ovarian-response-following-intra-ovarian-injection-of-platelet-rich-plasma-prp
#19
Marzie Farimani, Safoura Heshmati, Jalal Poorolajal, Maryam Bahmanzadeh
The prevalence of poor response to gonadotropin stimulation is approximately 9-24% in women undergoing in vitro fertilization. Interestingly, due to containing a variety of growth factors, platelet-rich plasma (PRP) can play an important role in tissue regeneration and healing. Thus, in this research, we aimed to investigate the intra-ovarian injection of PRP in women with poor ovarian response. To this goal, 23 poor responders constituted the study population, from among whom 19 women were enrolled. These patients underwent ovarian stimulation according to the Shanghai protocol...
February 5, 2019: Molecular Biology Reports
https://read.qxmd.com/read/30725184/intrafollicular-melatonin-concentration-is-elevated-in-patients-with-ovarian-hyperstimulation-syndrome-ohss-and-can-serve-as-an-important-predictor-of-ohss
#20
Min Zheng, Gaoge Zuo, Jing Tong, Zi-Jiang Chen, Wei-Ping Li, Cong Zhang
PURPOSE: Melatonin is an important factor in regulating numerous processes in human female reproduction. The aim of the present study was to compare melatonin levels in the follicular fluid (FF) of ovarian hyperstimulation syndrome (OHSS) women with those of non-OHSS women undergoing in vitro fertilization (IVF)-embryo transfer and to evaluate the relationship between FF melatonin levels and IVF outcomes in these women. METHODS: We determined FF melatonin levels in 20 OHSS women and 23 non-OHSS women on oocyte retrieval day...
February 6, 2019: Archives of Gynecology and Obstetrics
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