keyword
https://read.qxmd.com/read/38288089/swim-up-method-is-superior-to-density-gradient-centrifugation-for-preserving-sperm-dna-integrity-during-sperm-processing
#21
JOURNAL ARTICLE
Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao
PURPOSE: This study aimed to evaluate the effects of swim-up and density gradient centrifugation methods on sperm DNA fragmentation. METHODS: Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study. Sperm DNA fragmentation, progressive motility, and progressive motile sperm number were measured before and after the swim-up method or density gradient centrifugation. RESULTS: Sperm DNA fragmentation was not statistically different between swim-up-(14...
2024: Reproductive Medicine and Biology
https://read.qxmd.com/read/38282317/proteomic-analysis-of-boar-seminal-plasma-putative-markers-for-fertility-based-on-capacity-of-semen-preservation-at-17%C3%A2-c
#22
JOURNAL ARTICLE
Matheus S Lucca, Ivan C Bustamante-Filho, Rafael R Ulguim, Rafael D F Gianluppi, Joseph A M Evaristo, Fábio C S Nogueira, Luís F S M Timmers, Ana P G Mellagi, Ivo Wentz, Fernando P Bortolozzo
Boar seminal plasma (SP) proteins were associated with differences on sperm resistance to cooling at 17°C. However, information about seminal plasma proteins in boars classified by capacity of semen preservation and in vivo fertility remains lacking. Thus, the objective was to evaluate the SP proteome in boars classified by capacity of semen preservation and putative biomarkers for fertility. The ejaculates from high-preservation (HP) showed higher progressive motility during all 5 days than the low-preservation (LP) boars...
January 2024: Molecular Reproduction and Development
https://read.qxmd.com/read/38280300/vibration-emissions-affect-the-quality-of-liquid-preserved-ai-doses-in-stallions
#23
JOURNAL ARTICLE
Hannah Dierberger, Laura Pieper, Markus Jung, Martin Schulze
Artificial insemination (AI) with liquid-preserved stallion semen is a widely used reproductive technology. As the demand for AI doses of high-class stallions is transnational, they are frequently exposed to long-distance transport. Since recent studies in boars indicated that vibration emissions caused by transport negatively affected sperm quality in vitro, this study questioned whether sperm quality in stallions is similarly impaired. Furthermore, we investigated stallion and extender-related differences in the spermatozoa's resistance to transport-related quality loss...
January 17, 2024: Theriogenology
https://read.qxmd.com/read/38275779/astaxanthin-improved-the-quality-of-hu-ram-semen-by-increasing-the-antioxidant-capacity-and-mitochondrial-potential-and-mitigating-free-radicals-induced-oxidative-damage
#24
JOURNAL ARTICLE
Tariq Sohail, Liuming Zhang, Xuyang Wang, Caiyu Jiang, Jian Wang, Xiaomei Sun, Yongjun Li
The objective of this research was to investigate the effect of astaxanthin supplementations of semen extender on the quality of Hu ram semen after up to five days of preservation at 4 °C. Semen samples were collected from five healthy Hu rams using an artificial vagina during breeding season (April to August 2023) and diluted with a basic extender supplemented with control (0), 1 µM, 2 µM, 3.5 µM, or 4.5 µM of AXT. Overall, 170 semen ejaculate samples (34 repetitions) from five healthy Hu rams were used in our research study...
January 19, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38275778/punicalagin-protects-ram-sperm-from-oxidative-stress-by-enhancing-antioxidant-capacity-and-mitochondrial-potential-during-liquid-storage-at-4-%C3%A2-c
#25
JOURNAL ARTICLE
Liuming Zhang, Xuyang Wang, Tariq Sohail, Caiyu Jiang, Yuxuan Sun, Jian Wang, Xiaomei Sun, Yongjun Li
The aim of this study was to investigate the effect of punicalagin, an antioxidant, on ram sperm quality. Semen samples were collected and pooled from five rams, then diluted using a Tris-based diluent containing various concentrations (0, 5, 15, 30 and 45 μM) of punicalagin. Sperm motility, plasma membrane integrity, acrosome integrity, total antioxidant capacity (TAC), reactive oxygen species (ROS), malondialdehyde (MDA), mitochondrial membrane potential (MMP), superoxide dismutase (SOD) and catalase (CAT) were measured and analyzed during liquid storage at 4 °C...
January 19, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38271972/effect-of-codonopsis-pilosula-polysaccharide-on-the-quality-of-sheep-semen-preservation-at-4%C3%A2-c
#26
JOURNAL ARTICLE
Yuqin Wang, Yanhong Zhao, Huan Chen, Tingting Lu, Rujie Yang, Xiuxiu Weng, Wanhong Li
OBJECTIVE: This study aimed to investigate the effect of Codonopsis pilosula polysaccharide (CPP) on the motility, mitochondrial integrity, acrosome integrity rate, and antioxidant ability of sheep sperm after preservation at 4 °C. METHODS: Semen from healthy adult rams were collected and divided into four groups with separate addition of 0, 200, 400, and 1000 mg/L CPP. Sperm motility was analyzed using the Computer-Assisted Semen Analysis software after preservation at 4 °C for 24, 72, 120, and 168 h...
January 20, 2024: Animal bioscience
https://read.qxmd.com/read/38271968/antioxidant-activity-and-metabolic-regulation-of-sodium-salicylate-on-goat-sperm-at-low-temperature
#27
JOURNAL ARTICLE
Wenzheng Shen, Yu Fu, Haiyu Bai, Zhiyu Zhang, Zhikun Cao, Zibo Liu, Chao Yang, Shixin Sun, Lei Wang, Chunhuan Ren, Yinghui Ling, Zijun Zhang, Hongguo Cao
OBJECTIVE: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats. METHODS: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics. RESULTS: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 µM SS group was 66...
January 20, 2024: Animal bioscience
https://read.qxmd.com/read/38268212/quality-and-redox-state-of-bovine-sperm-cryopreserved-with-resveratrol-use-of-resveratrol-in-bovine-semen
#28
JOURNAL ARTICLE
Felipe Correa, Esteban Ceballos, Benjamín Rojano, Giovanni Restrepo, Alexandra Usuga
The use of antioxidants for semen preservation prevents oxidative damage caused by reactive oxygen species (ROS). One of the most promising natural antioxidants is resveratrol, a phytoalexin derived from plants, grapes, berries, peanuts and red wine. To evaluate the effect of resveratrol on the quality and redox status of cryopreserved bovine semen. Five bulls were subjected to electroejaculation to obtain 15 ejaculates. Each ejaculate was extended with a tris-egg yolk-glycerol-based medium and divided into six aliquots supplemented with 0 (control), 10, 20, 30, 40 and 50 μM of resveratrol...
January 2024: Reproduction in Domestic Animals
https://read.qxmd.com/read/38267808/effect-of-five-different-antioxidants-on-the-effectiveness-of-goat-semen-cryopreservation
#29
JOURNAL ARTICLE
Xiaohua Yi, Yanbo Qiu, Xiaoqin Tang, Yichen Lei, Yun Pan, Sayed Haidar Abbas Raza, Norah A Althobaiti, Aishah E Albalawi, Waleed Al Abdulmonem, Raafat T M Makhlof, Mohammad A Alsaad, Yu Zhang, Xiuzhu Sun
The effective combination of semen cryopreservation and artificial insemination has a positive effect on the conservation of germplasm resources, production and breeding, etc. However, during the process of semen cryopreservation, the sperm cells are very susceptible to different degrees of physical, chemical, and oxidative stress damage. Oxidative damage is the most important factor that reduces semen quality, which is affected by factors such as dilution equilibrium, change of osmotic pressure, cold shock, and enzyme action during the freezing-thawing process, which results in the aggregation of a large amount of reactive oxygen species (ROS) in sperm cells and affects the quality of semen after thawing...
January 24, 2024: Reproductive Sciences
https://read.qxmd.com/read/38265832/evaluation-of-cryopreserved-ram-sperm-with-nano-ozone-solution-and-post-thaw-life-span-by-flow-cytometric-analysis
#30
JOURNAL ARTICLE
Mehmed Berk Toker, Ahmet Ümit Sabancı, Gülcan Avcı, Ahmet Aktar, Barış Denk, Özge Bari, Gözde Rabia Özalp
Ozone has been used as a therapy tool in medical science for conditions such as ulcers, peritonitis, wounds, and mostly joint problems. Ozone therapy strengthens the resistance to infections by kick-starting antioxidant, anti-inflammatory, and immune modulation systems. Ozone creates a defensive response against oxidative stress in membranes and protects metabolism against reactive oxygen species (ROS). Sperm membranes are one of ROS's main targets; therefore, the cells' cryopreservation process requires more defensive elements for better results...
January 24, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38265831/cryopreservation-induced-morphological-changes-in-freshwater-fish-sperm-a-systematic-review
#31
JOURNAL ARTICLE
Bruna Bitencourt da Costa, Paula Graziela Lassen, Danilo Pedro Streit
A systematic review was performed to summarize the scientific evidence and critically evaluate the effects of cryopreservation on sperm morphology in freshwater fish, and to assess the methodologies for sperm morphology classification. The search strategy was applied to four electronic databases (CAB Direct, Pub Med, Scopus, and ISI Web of Science). The main inclusion criteria involved studies on semen from freshwater fish subjected to the cryopreservation process and evaluation of sperm quality through morphology...
January 24, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38263469/roles-of-y-27632-on-sheep-sperm-metabolism
#32
JOURNAL ARTICLE
Yu Fu, Wenzheng Shen, Haiyu Bai, Zhiyu Zhang, Zhikun Cao, Zibo Liu, Chao Yang, Shixin Sun, Lei Wang, Yinghui Ling, Zijun Zhang, Hongguo Cao
To investigate the effect of Y-27632 on low-temperature metabolism of sheep sperm, different concentrations of Y-27632 were added to sheep semen at 4 ℃ in this experiment to detect indicators such as sperm motility, plasma membrane, acrosome, antioxidant performance, mitochondrial membrane potential (MMP), and metabolomics. The results showed that the addition of 20 µM Y-27632 significantly increased sperm motility, plasma membrane integrity rate, acrosome integrity rate, antioxidant capacity, MMP level, significantly increased sperm ATP and total cholesterol (TC) content, and significantly reduced sperm Ca2+ content...
January 23, 2024: Journal of Animal Science
https://read.qxmd.com/read/38247528/pyrroloquinoline-quinone-improves-ram-sperm-quality-through-its-antioxidative-ability-during-storage-at-4-%C3%A2-c
#33
JOURNAL ARTICLE
Zhendong Zhu, Wenjia Li, Qitai Yang, Haolong Zhao, Weijing Zhang, Adedeji O Adetunji, S A Masudul Hoque, Xin Kou, Lingjiang Min
Sperm motility is an important factor in the migration of sperm from the uterus to the oviduct. During sperm preservation in vitro, sperm generates excessive ROS that damages its function. This study aims to investigate whether the addition of pyrroloquinoline quinone (PQQ) to the diluted medium could improve chilled ram sperm quality, and then elucidates the mechanism. Ram semen was diluted with Tris-citric acid-glucose (TCG) medium containing different doses of PQQ (0 nM, 10 nM, 100 nM, 1000 nM, 10,000 nM), and stored at 4 °C...
January 15, 2024: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38242354/pubertal-status-at-time-of-fertility-preservation-in-transgender-girls
#34
JOURNAL ARTICLE
Mabel Yau, Neha R Malhotra, John Steever, Eva Baldisserotto, Cathy Lin, Ralph Fader, Matthew Oransky, Joshua D Safer
BACKGROUND: Puberty blockade and gender affirming hormone therapy can impair fertility. Counseling on fertility preservation is important prior to initiation of therapy. The aim of our study was to assess tanner staging and the presence of virilizing secondary sex characteristics at the time of sperm collection and to correlate the viability of sperm with the tanner staging and degree of virilization. METHODS: A retrospective chart review of 23 transgender girls referred to pediatric urology at our academic pediatric transgender clinic for fertility preservation counseling was performed...
January 17, 2024: Endocrine Practice
https://read.qxmd.com/read/38242276/effects-of-alpha-lipoic-acid-supplementation-on-post-thaw-quality-of-drone-semen
#35
JOURNAL ARTICLE
Ahmet Aktar, Selvinar Çakmak, Mehmed Berk Toker, İbrahim Çakmak, Kemal Bağci, Ümit Can Uzun, Selim Alçay
This study aimed to determine the effect of alpha-lipoic acid (ALA) on post-thaw quality of bee semen. In the study, semen from sexually mature drone were collected. A series of experiments were carried out in which the retrieved semen was diluted with diluents containing different ALA concentrations or without ALA supplement (control). Cryopreserved sperm were thawed, and evaluated for motility (phase-contrast microscope), plasma and acrosomal membrane integrity, mitochondrial membrane potential, and DNA fregmantation...
January 17, 2024: Cryobiology
https://read.qxmd.com/read/38237325/exploring-how-sucrose-colloid-selection-improves-the-fertilizing-ability-of-chicken-sperm-after-cryopreservation-with-glycerol
#36
JOURNAL ARTICLE
Hsiu-Lien Herbie Lin, Pascal Mermillod, Isabelle Grasseau, Elisabeth Blesbois, Anaïs Vitorino Carvalho
Currently, glycerol is the most effective cryoprotectant when combined with straw packaging for preserving chicken sperm. Glycerol, however, has toxic effects on sperm cells, which can reduce fertility when present in inseminated semen. Historically, the serial dilution (SD) method was developed to eliminate glycerol and mitigate its adverse effects. We have recently developed a new method for removing glycerol called sucrose-Percoll (SP), that can be performed at either 4°C (4°C-SP) or 20°C (20°C-SP)...
January 10, 2024: Poultry Science
https://read.qxmd.com/read/38220340/investigating-the-cryoprotective-efficacy-of-fructans-in-mammalian-cell-systems-via-a-structure-functional-perspective
#37
JOURNAL ARTICLE
Selay Tornacı, Merve Erginer, Barış Gökalsın, Arzu Aysan, Metin Çetin, Mubarak Sadauki, Necati Fındıklı, Seval Genç, Cenk Sesal, Ebru Toksoy Öner
Fructans have long been known with their role in protecting organisms against various stress factors due to their ability to induce controlled dehydration and support membrane stability. Considering the vital importance of such features in cryo-technologies, this study aimed to explore the cryoprotective efficacy of fructans in mammalian cell systems where structurally different fructan polymers were examined on in vitro cell models derived from organs such as the liver, frequently used in transplantation, osteoblast, and cord cells, commonly employed in cell banking, as well as human seminal fluids that are of vital importance in assisted reproductive technology...
March 15, 2024: Carbohydrate Polymers
https://read.qxmd.com/read/38207248/oncofertility-and-pregnancy-in-adolescent-and-young-adult-cancers-physicians-knowledge-and-preferences-in-india
#38
JOURNAL ARTICLE
Sharada Mailankody, Jyoti Bajpai, Puneet R Arora, Rinoy Sreedharan, Prakash Chitalkar, Purna Kurkure, Hemant Malhotra, Firuza R Parikh, Sudeep Gupta, Shripad D Banavali
PURPOSE: The treatment outcomes of adolescent and young adult (AYA) cancers have improved with advanced oncology care. Hence, fertility preservation (FP) and post-therapy pregnancies (PTPs) become vital issues. MATERIALS AND METHODS: An online survey link with 17 questions regarding oncofertility and PTPs was circulated among oncologists to assess the knowledge, understand the oncofertility care patterns, and seek suggestions to improve oncofertility services. RESULTS: The median age of 179 respondents, predominantly medical oncologists (68...
January 2024: JCO global oncology
https://read.qxmd.com/read/38203631/the-role-of-microrna-in-spermatogenesis-is-there-a-place-for-fertility-preservation-innovation
#39
REVIEW
Charlotte Klees, Chrysanthi Alexandri, Isabelle Demeestere, Pascale Lybaert
Oncological treatments have dramatically improved over the last decade, and as a result, survival rates for cancer patients have also improved. Quality of life, including concerns about fertility, has become a major focus for both oncologists and patients. While oncologic treatments are often highly effective at suppressing neoplastic growth, they are frequently associated with severe gonadotoxicity, leading to infertility. For male patients, the therapeutic option to preserve fertility is semen cryopreservation...
December 29, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38202772/metabolomics-analysis-of-sodium-salicylate-improving-the-preservation-quality-of-ram-sperm
#40
JOURNAL ARTICLE
Haiyu Bai, Zhiyu Zhang, Wenzheng Shen, Yu Fu, Zhikun Cao, Zibo Liu, Chao Yang, Shixin Sun, Lei Wang, Yinghui Ling, Zijun Zhang, Hongguo Cao
The aim of this study was to investigate the effects of sodium salicylate (SS) on the preservation and metabolic regulation of sheep sperm. Under 4 °C low-temperature conditions, SS (at 10 µM, 20 µM, 30 µM, and 50 µM) was added to the semen diluent to detect sperm motility, plasma membrane, and acrosome integrity. Based on the selected optimal concentration of SS (20 µM), the effects of 20 µM of SS on sperms' antioxidant capacity and mitochondrial membrane potential (MMP) were evaluated, and metabolomics analysis was conducted...
December 28, 2023: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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