Yu Fu, Wenzheng Shen, Haiyu Bai, Zhiyu Zhang, Zhikun Cao, Zibo Liu, Chao Yang, Shixin Sun, Lei Wang, Yinghui Ling, Zijun Zhang, Hongguo Cao
To investigate the effect of Y-27632 on low-temperature metabolism of sheep sperm, different concentrations of Y-27632 were added to sheep semen at 4 ℃ in this experiment to detect indicators such as sperm motility, plasma membrane, acrosome, antioxidant performance, mitochondrial membrane potential (MMP), and metabolomics. The results showed that the addition of 20 µM Y-27632 significantly increased sperm motility, plasma membrane integrity rate, acrosome integrity rate, antioxidant capacity, MMP level, significantly increased sperm ATP and total cholesterol (TC) content, and significantly reduced sperm Ca2+ content...
January 23, 2024: Journal of Animal Science