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Semen preservation

James Logue, Walter Vargas Licona, Timothy K Cooper, Becky Reeder, Russel Byrum, Jing Qin, Nicole Deiuliis Murphy, Yu Cong, Amanda Bonilla, Jennifer Sword, Wade Weaver, Gregory Kocher, Gene G Olinger, Peter B Jahrling, Lisa E Hensley, Richard S Bennett
Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectious virus. Virus isolation, typically using cell culture or animal models, can serve as a tool to determine the infectivity of patient samples. However, the sensitivity of these methods has not been assessed for the Ebola virus isolate, Makona...
February 16, 2019: Viruses
Ruhollah Rahimi, Hamid Farahmand, Alireza Mirvaghefi, Gholamreza Rafiee, Amirreza Abed-Elmdoust
Persian sturgeon (Acipenser persicus) is an endangered species and genetic resource banking such as gametes and embryo preservation could be one of the most pursued conservation approaches. In this study, deleterious effects of the traditional cryopreservation technique and the effect of different doses of 2-hydroxypropyl-beta-cyclodextrin (HβCD) on thawed spermatozoa quality (motility duration and percentage) of Persian sturgeon (Acipenser persicus) were investigated from metabolic aspects of view. For cryopreserving, semen was diluted with Tris-HCl (100 mM) extenders containing 0, 5, 10, and 15 mM of HβCD in a ratio of 1:1 (semen/extenders)...
February 18, 2019: Fish Physiology and Biochemistry
Joshua A Halpern, Nannan Thirumavalavan, Taylor P Kohn, Amir S Patel, Joon Yau Leong, Raimondo M Cervellione, David J B Keene, Emad Ibrahim, Nancy L Brackett, Dolores J Lamb, Ranjith Ramasamy
OBJECTIVES: To determine the distribution of semen parameters among adolescent and adult males presenting for fertility preservation. METHODS: A retrospective, cross-sectional cohort study of adolescent males age 11-19 who underwent semen analysis (SA) for fertility preservation at three centers in two countries with a comparison cohort of adult men presenting for fertility preservation. Prevalence of azoospermia and distribution of semen parameters was compared across groups...
February 13, 2019: Urology
Senay Cankut, Turgay Dinc, Mehmet Cincik, Guler Ozturk, Belgin Selam
AIM: Human sperm DNA fragmentation is one of the factors suggested for male infertility. The ratio of sperm DNA damage in semen may adversely affect both the fertilization rate and the embryo development of in vitro fertilization/ intracytoplasmic sperm injection cycles. Sperm cryopreservation both increases the success rates in assisted reproductive techniques (ARTs) and contributes to the preservation of fertility before testis surgery, chemotherapy, and radiotherapy. The aim of the current study is to determine sperm DNA fragmentation, following cryopreservation...
February 4, 2019: Reproductive Sciences
Luisa Zupin, Vania Polesello, Monica Martinelli, Stefania Luppi, Elena Giolo, Gabriella Zito, Federico Romano, Ludovica Segat, Sergio Crovella, Giuseppe Ricci
PURPOSE: β-defensins are antimicrobial peptides expressed at mucosal level of male and female genito-urinary tract, where they exert protective functions against infections, possibly preserving human health and fertility. In our study, we investigated the possible involvement of β-defensins in female and male infertility in Italian infertile couples (i) evaluating the presence of human β-defensin 1 (hBD-1) in follicular fluid (FF) and its correlation with in vitro fertilization (IVF) outcomes; (ii) investigating the relationship between hBD-1 levels in semen and IVF outcomes (comprising correlation with sperm parameters); and (iii) exploring the effect of hBD-1 peptide on spermatozoa motility in vitro...
February 2, 2019: Journal of Assisted Reproduction and Genetics
Gui-Chao Dai, Yu Meng, Li-Kun Zhang, Ye-Qing Du, Fei Wen, Tian-Yu Feng, Jian-Hong Hu
Adding a certain amount of antioxidants to semen extender has been shown to improve semen quality. The aim of present study was to elucidate whether the supplementation of melatonin to the Tris-based extender (CTR) could enhance the quality of ram spermatozoa during storage at 4°C. Ram semen samples were collected and diluted with CTR extender containing different concentrations (0, 0.05 (M 0.05), 0.1 (M 0.1), 0.2 (M 0.2) or 0.4 (M 0.4) mM) of melatonin. Sperm routine indicators, mitochondrial activity, total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content were analysed in control and melatonin treatment groups...
January 29, 2019: Andrologia
Tessa Brik, Lieke J J J Vrouenraets, Sebastian E E Schagen, Andreas Meissner, Martine C de Vries, Sabine E Hannema
PURPOSE: The primary aims of the study are to examine the rate of attempted fertility preservation (FP) among a Dutch cohort of transgirls who started gonadotropin-releasing hormone analog treatment and the reasons why adolescents did or did not choose to attempt FP. METHODS: The study was a single-center retrospective review of medical records of 35 transgirls who started gonadotropin-releasing hormone analog treatment between 2011 and 2017. RESULTS: Ninety-one percent of adolescents were counseled on the option of FP...
January 25, 2019: Journal of Adolescent Health: Official Publication of the Society for Adolescent Medicine
Walid S El-Nattat, Reda I El-Sheshtawy
Strawberry fruit is rich in natural antioxidants supplemented as a preservative in semen extenders. There is little literature regarding its application in cattle bulls. So, the aim of the present study was to explore the impact of strawberry juice beside LDL derived from egg yolk on buffalo spermatozoa during cooling and cryopreservation. Collected semen was pooled to be divided in aliquots. Semen was diluted in tris-Citrate-Fructose-yolk low-density lipoprotein (TLCF) (control, 0% strawberry juice (SB)) and in six different concentrations (1-6%) of tris-LDL-strawberry diluent (TLSB) to ensure 60 million motile spermatozoa/mL...
January 28, 2019: Tropical Animal Health and Production
M Skott, H Schrøder, J Hindkjaer, H J Kirkeby
BACKGROUND: This report presents a case where electroejaculation (EEJ) was used for semen cryopreservation (SCP), prior to gonadotoxic anti-cancer treatment in a 14-year old boy diagnosed with Hodgkins disease. METHOD: Two sessions of EEJ were performed with an interval of 48 hours. RESULTS: No complications were seen and the procedures resulted in nine frozen straws of motile spermatozoa. CONCLUSION: EEJ is a safe and feasible procedure for SCP in an adolescent cancer patient who is unable to masturbate or use penile vibratory stimulation (PVS)...
January 17, 2019: Scandinavian Journal of Urology
Minh Tam Le, Thi Thai Thanh Nguyen, Thanh Tung Nguyen, Van Trung Nguyen, Thi Tam An Nguyen, Vu Quoc Huy Nguyen, Ngoc Thanh Cao
OBJECTIVES: Sperm cryopreservation has great potential for male infertility treatment as used in assisted reproduction technology (ART). There are a variety of cryopreservation methods in order to preserve sperm in a long term. Although conventional freezing and vitrification now are used widely, they have damage on sperm parameters as well as sperm DNA integrity. It is necessary to answer which method is better and appropriate for sperm cryopreservation. The aim of this study was to compare the effect of conventional freezing and vitrification regarding to motility, vitality and morphology of sperm found in washed and unwashed samples...
January 5, 2019: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Tânia R Dias, Luna Samanta, Ashok Agarwal, Peter N Pushparaj, Manesh Kumar Panner Selvam, Rakesh Sharma
Elevated levels of reactive oxygen species (ROS) are a major cause of male infertility. However, some men with high seminal ROS levels are still fertile. The main objective of this study was to understand the molecular mechanism(s) responsible for the preservation of fertility in those men. Semen samples from fertile men were divided into two groups: control ( n = 10, ROS < 102.2 RLU/s/10⁶ sperm) and ROS+ ( n = 10, ROS > 102.2 RLU/s/10⁶ sperm). Proteomic analysis of seminal plasma and spermatozoa was used to identify the differentially expressed proteins (DEPs) between the experimental groups, from which some proteins were validated by Western blot (WB)...
January 8, 2019: International Journal of Molecular Sciences
K Almekaty, M H Zahran, A Zoeir, S Minhas, K Salem
BACKGROUND: There is wide agreement nowadays that a clinical varicocoele should be ligated to treat male factor infertility. However, the significance of testicular artery preservation in patients with severe oligozoospermia has not been addressed before. OBJECTIVES: To assess the outcome of varicocelectomy in infertile men with severe oligozoospermia and clinical varicocoeles and to compare internal spermatic artery preservation vs. artery ligation. MATERIALS AND METHODS: This prospective randomized study included 302 infertile patients with severe oligozoospermia and clinical (grade II/III) varicocoeles...
January 7, 2019: Andrology
Ehab Elkady, Tamer Dawod, Mohamed Teleb, Waleed Shabana
OBJECTIVE: To compare outcome of muscle and nerve-sparing bulbar urethroplasty with standard bulbar urethroplasty as regard ejaculatory dysfunction and post-void dribbling. METHODS: This prospective randomized study included 50 patients with bulbar urethral stricture underwent urethroplasty over a period of 5y. All patients were operated by ventral onlay buccal mucosal graft urethroplasty and randomly divided into two groups. Group I (n=25) was operated by standard bulbar urethroplasty...
December 31, 2018: Urology
Luana G P Bezerra, Ana L P Souza, Arthur E A Lago, Livia B Campos, Talita L Nunes, Valéria V Paula, Moacir F Oliveira, Alexandre R Silva
The effect of Equex STM® paste supplementation on the Tris-extender for collared peccaries' semen cryopreservation was assessed. Semen from 12 mature individuals was obtained by electroejaculation and evaluated for morphology, membrane integrity, osmotic response, and sperm kinetic metrics. Samples were diluted in Tris plus 20% egg yolk and divided into three aliquots. The first aliquot was without any supplementation, the second and third contained 0.5 and 1.0% Equex STM, respectively. The samples were added with 3% glycerol, frozen in liquid nitrogen, thawed, and assessed for the same parameters after a thermal resistance test (TRT) for 120 minutes...
January 2, 2019: Biopreservation and Biobanking
Ryan Dosselli, Julia Grassl, Susanne P A den Boer, Madlen Kratz, Jessica M Moran, Jacobus J Boomsma, Boris Baer
All social insects with obligate reproductive division of labour evolved from strictly monogamous ancestors, but multiple queen-mating (polyandry) arose de novo in several evolutionarily derived lineages. Polyandrous ant queens are inseminated soon after hatching and store sperm mixtures for a potential reproductive life of decades. However, they cannot re-mate later in life and are thus expected to control the loss of viable sperm because their life-time reproductive success is ultimately sperm-limited. In the leaf-cutting ant Atta colombica, the survival of newly inseminated sperm is known to be compromised by seminal fluid of rival males and to be protected by secretions of the queen sperm storage organ (spermatheca)...
December 31, 2018: Molecular & Cellular Proteomics: MCP
Favián Treulen, Luis Aguila, María Elena Arias, Ignacio Jofré, Ricardo Felmer
During cryopreservation procedures, the spermatozoa are exposed to physical and chemical stressors that generate an increase in the intracellular concentration of reactive oxygen species (ROS). If ROS concentrations are too great, this can lead to a state of oxidative stress that are detrimental to sperm quality. The aim of this study was to ascertain the profile the ROS production and assess the effects of post-thaw supplementation of a semen extender with different antioxidant compounds on the quality and function variables of frozen-thawed stallion spermatozoa incubated in vitro...
December 20, 2018: Animal Reproduction Science
Seung Hun Song, Dae Keun Kim, Su Ye Sung, Young Sun Her, Ok Hee Lee, Myoung Hwa Choi, Hae Kyung Kim, Sang Woo Lyu, Dong Suk Kim
PURPOSE: Sperm cryopreservation before cancer treatment is the most effective method to preserve the fertility of male patients. We present our 21 years experience with sperm cryopreservation for cancer patients, including an examination of semen quality, the current status of cryopreserved sperm, and the rate of sperm use for assisted reproductive technology (ART). MATERIALS AND METHODS: A total of 721 cancer patients at Fertility Center of CHA Gangnam Medical Center successfully performed sperm cryopreservation for fertility preservation from January 1996 to December 2016...
December 26, 2018: World Journal of Men's Health
Yogesh Soni, T R Talluri, Ashok Kumar, S K Ravi, J S Mehta, B N Tripathi
Traditionally Glycerol (Gly) is being used as major cryoprotectant and its toxicity could be a reason for the variation on stallion sperm freezability and fertility. In an effort to minimize Gly toxicity alternative cryoprotective agents like Di-methyl Formamide (DMF) have been investigated. The effect of the cryoprotectant and dose of cryoprotective agent varies from breed to breed and also from stallion to stallion within the same breed. Considering these factors a study was designed to study the effects of Gly and DMF at different concentrations and combinations on the plasma membrane, acrosome and DNA integrity as well as other post thaw seminal characteristics of semen of three Indigenous stallion breeds...
December 18, 2018: Cryobiology
F Pezo, F Romero, F Zambrano, R Sánchez
In the pork industry, artificial insemination and the storage of boar semen in liquid at 17°C are routinely applied to optimize the ejaculate and bring about rapid genetic changes that are reflected in the animal protein. Although the results are satisfactory, they are below what occurs with natural mating. It is currently possible to preserve boar semen with storage at 17°C and slow freezing, since to date there is only one study on vitrification, with negative results applicable only in the case of implementing an intracytoplasmic sperm injection...
December 8, 2018: Reproduction in Domestic Animals, Zuchthygiene
Karen M Wheeler, Devang Sharma, Parviz K Kavoussi, Ryan P Smith, Raymond Costabile
INTRODUCTION: Clomiphene citrate (CC) is a selective estrogen receptor modulator that has been used for the treatment of hypogonadism in men since the 1970s. It acts centrally to increase secretion of luteinizing hormone and follicle-stimulating hormone, thereby increasing testosterone production and serum levels. Unlike testosterone replacement therapy, CC does not suppress the hypothalamic-pituitary-gonadal axis, preserving intratesticular testosterone production and spermatogenesis...
December 3, 2018: Sexual Medicine Reviews
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