Read by QxMD icon Read

Amniotic tissue

Saba Aslani, Mahboubeh Kabiri, Mousa Kehtari, Hana Hanaee-Ahvaz
As the incidence of small-diameter vascular graft (SDVG) occlusion is considerably high, a great amount of research is focused on constructing a more biocompatible graft. The absence of a biocompatible surface in the lumen of the engineered grafts that can support confluent lining with endothelial cells (ECs) can cause thrombosis and graft failure. Blood clot formation is mainly because of the lack of an integrated endothelium. The most effective approach to combat this problem would be using natural extracellular matrix constituents as a mimic of endothelial basement membrane along with applying anticoagulant agents to provide local antithrombotic effects...
February 18, 2019: Journal of Cellular Physiology
Carlota Rodó, Anna Suy, Elena Sulleiro, Antoni Soriano-Arandes, Nerea Maiz, Itziar García-Ruiz, Silvia Arévalo, Ariadna Rando, Andrés Anton, Élida Vázquez Méndez, Marta Garrido, Antoinette Frick, Carlos Rodrigo, Tomás Pumarola, Elena Carreras
OBJECTIVES: to describe pregnancy outcomes after Zika virus (ZIKV) infection in a non-endemic region. METHODS: according to the Spanish protocol issued after the ZIKV outbreak in Brazil in 2015, all pregnant women who had travelled to high-burden countries were screened for ZIKV. Serological and molecular tests were used to identify ZIKV-infected pregnant women. They were classified as confirmed ZIKV infection when reverse-transcriptase polymerase chain reaction (RT-PCR) tested positive, or probable ZIKV infection when ZIKV-immunoglobulin M and/or immunoglobulin G and ZIKV plaque reduction neutralization tests were positive...
February 13, 2019: Clinical Microbiology and Infection
Sally L Baxter, Brian J Nguyen, Michael Kinori, Don O Kikkawa, Shira L Robbins, David B Granet
PURPOSE: To report the characteristics of patients with restrictive diplopia following pterygium excision and a successful treatment approach for the strabismus. DESIGN: Retrospective interventional case series. METHODS SETTING: Single academic institution. PATIENT POPULATION: Fifteen patients with restrictive diplopia after pterygium excision. EXCLUSION CRITERIA: any other reason for strabismus...
February 13, 2019: American Journal of Ophthalmology
David Fernandez
An 87-year old male received surgical excision of scalp melanoma and subsequent radiotherapy due to metastasis to the skull. A radiation-induced wound developed with osteoradionecrosis that required necrotic bone excision resulting in a 7.5x8.5cm wound over the exposed dura, which remained non-healing despite many attempts by local wound care management. Due to the refractory nature of the wound, strips of cryopreserved umbilical cord (cUC) allograft were applied over the exposed dura resulting in significant vascular granulation tissue formation in the central wound bed within four weeks...
February 1, 2019: Journal of Wound Care
Samuel John, Marco Rainer Kesting, Philipp Paulitschke, Mechthild Stöckelhuber, Achim von Bomhard
Allogenic graft material and tissue engineering have recently shown promising results for the improvement of both esthetic and functional outcomes in the treatment of large skin defects. We chose human amniotic membrane as a cellular scaffold in order to develop a skin substitute for later in vivo uses. Various methods of de-epithelialization of the human amniotic membrane were evaluated by histological analysis including hematoxylin-eosin and laminin staining, optic coherence tomography, and scanning electron microscopy with 0...
January 2019: Journal of Tissue Engineering
Reza Khorramirouz, Seyedeh M Kameli, Kiarad Fendereski, Seyedeh S Daryabari, Abdol-Mohammad Kajbafzadeh
AIM: The aim of this study was to evaluate the efficacy of tissue-engineered amniotic membrane (AM) in the treatment of myocardial infarction lesions. MATERIALS & METHODS: 20 rats were subjected to coronary arterial ligation in order to induce myocardial infarction injury. Decellularized human AMs were seeded with 2 × 105 adipose-derived mesenchymal stem cells and were implanted in the infarcted hearts. RESULTS & CONCLUSION: Histological and immunohistochemical evaluations indicated the regeneration of cardiomyocytes and reduction of inflammation and fibrosis in the patch-implanted group compared with a control group, 14 days after the surgery...
February 11, 2019: Regenerative Medicine
Sota Iwatani, Makiko Yoshida, Keiji Yamana, Daisuke Kurokawa, Jumpei Kuroda, Khin Kyae Mon Thwin, Suguru Uemura, Satoru Takafuji, Nanako Nino, Tsubasa Koda, Masami Mizobuchi, Masahiro Nishiyama, Kazumichi Fujioka, Hiroaki Nagase, Ichiro Morioka, Kazumoto Iijima, Noriyuki Nishimura
Mesenchymal stem cells (MSCs) have considerable therapeutic potential and attract increasing interest in the biomedical field. MSCs are originally isolated and characterized from bone marrow (BM), then acquired from tissues including adipose tissue, synovium, skin, dental pulp, and fetal appendages such as placenta, umbilical cord blood (UCB), and umbilical cord (UC). MSCs are a heterogeneous cell population with the capacity for (1) adherence to plastic in standard culture conditions, (2) surface marker expression of CD73+ /CD90+ /CD105+ /CD45- /CD34- /CD14- /CD19- /HLA-DR- phenotypes, and (3) trilineage differentiation into adipocytes, osteocytes, and chondrocytes, as currently defined by the International Society for Cellular Therapy (ISCT)...
January 26, 2019: Journal of Visualized Experiments: JoVE
Laura S Gammill, Bridget Jacques-Fricke, Julaine Roffers-Agarwal
The ability to combine embryological manipulations with gene function analysis in an amniote embryo makes the chick a valuable system for the vertebrate developmental biologist. This chapter describes methods for those unfamiliar with the chick system wishing to initiate experiments in their lab. After outlining methods to prepare chick embryos, protocols are provided for introducing beads or cells expressing secreted factors, and for culturing tissue explants as a means of assessing development in vitro. Approaches to achieve gain of function and loss of function (morpholino oligonucleotides) in chick are outlined, and methods for introducing these reagents by electroporation are detailed...
2019: Methods in Molecular Biology
Xin Che, Han Wu, Changkai Jia, Huimin Sun, Shangkun Ou, Junqi Wang, M Vimalin Jeyalatha, Xin He, Jingwen Yu, Chengyou Zuo, Zuguo Liu, Wei Li
Purpose: To investigate a novel strategy in constructing tissue-engineered corneal stromal equivalent based on amniotic membrane and keratocytes. Methods: The ultrathin amniotic membrane (UAM) was laminated, with corneal stromal cells (CSCs) distributed between the space of the layered UAMs. Calcein AM staining was used to evaluate cellular viability, morphology, and arrangement. Immunostaining, qRT-PCR, and Western blot were performed to detect gene and protein expression in keratocytes...
February 1, 2019: Investigative Ophthalmology & Visual Science
Baozhi Ding, Xin Wang, Min Yao
PURPOSE: We utilized a novel approach of combined photochemical tissue bonding (PTB) and human amniotic membrane (HAM) to improve hand tendon repair and also evaluated its efficacy. METHODS: Subei chickens underwent surgical transection of the flexor digitorum profundus tendons and repair by (1) SR (standard Kessler suture; n = 24; 6-0 prolene) and (2) HAM/PTB (n = 24), where a section of HAM was stained with 0.1% Rose Bengal, wrapped around the ruptured tendon and bonded with 532 nm light (0...
January 30, 2019: Surgical Innovation
François Friocourt, Peter Kozulin, Morgane Belle, Rodrigo Suárez, Nicolas Di-Poï, Linda J Richards, Paolo Giacobini, Alain Chédotal
In Bilaterians, commissural neurons project their axons across the midline of the nervous system to target neurons on the opposite side. In mammals, midline crossing at the level of the hindbrain and spinal cord requires the Robo3 receptor which is transiently expressed by all commissural neurons. Unlike other Robo receptors, mammalian Robo3 receptors do not bind Slit ligands and promote midline crossing. Surprisingly, not much is known about Robo3 distribution and mechanism of action in other vertebrate species...
January 29, 2019: Journal of Comparative Neurology
James Bardill, Sarah M Williams, Uladzimir Shabeka, Lee Niswander, Daewon Park, Ahmed I Marwan
BACKGROUND: Myelomeningocele (MMC) results in lifelong neurologic and functional deficits. Currently, prenatal repair of MMC closes the defect, resulting in a 50% reduction in postnatal ventriculoperitoneal shunting. However, this invasive fetal surgery is associated with significant morbidities to mother and baby. We have pioneered a novel reverse thermal gel (RTG) to cover MMC defects in a minimally invasive manner. Here, we test in-vitro RTG long-term stability in amniotic fluid and in vivo application in the Grainy head-like 3 (Grhl3) mouse MMC model...
March 2019: Journal of Surgical Research
John D M Campbell, Sajjad Ahmad, Ashish Agrawal, Carol Bienek, Anne Atkinson, Neil W A Mcgowan, Stephen Kaye, Sanjay Mantry, Kanna Ramaesh, Alison Glover, Jane Pelly, Coral MacRury, Margaret MacDonald, Emily Hargreaves, Jacqueline Barry, John Drain, Bruce Cuthbertson, Louis Nerurkar, Ian Downing, Alasdair R Fraser, Marc L Turner, Baljean Dhillon
Limbal stem cell deficiency (LSCD) is a disease resulting from the loss or dysfunction of epithelial stem cells, which seriously impairs sight. Autologous limbal stem cell transplantation is effective in unilateral or partial bilateral disease but not applicable in total bilateral disease. An allogeneic source of transplantable cells for use in total bilateral disease can be obtained from culture of donated cadaveric corneal tissue. We performed a controlled multicenter study to examine the feasibility, safety, and efficacy of allogeneic corneal epithelial stem cells in the treatment of bilateral LSCD...
January 28, 2019: Stem Cells Translational Medicine
Emrah Erdoğan, Merve Yürük, Eda Sivcan, Serkan Karaca, Hanife Temel, Tuba Şabanoğlu, Aslıhan Gültekin, İzzet Şahin
Toxoplasma gondii is a compulsory intracellular protozoan parasite with a wide range of host in warm-blooded vertebrates and has importance in terms of health and economy. Toxoplasmosis is very common because it can infect people with a variety of ways; ingestion of contaminated water and nutrients; raw or undercooked meats containing tissue cysts, blood transfusions, organ transplantantation and transplacental transfer. The aim of this study was to evaluate serologic and molecular test results of toxoplasmosis pre-diagnosed patients...
January 2019: Mikrobiyoloji Bülteni
Lorenzo Alibardi, Victor Benno Meyer-Rochow
The process of tail regeneration in the tuatara (Sphenodon punctatus) is not entirely known. Similarity to and differences from lizard tail regenerations are indicated in the present histological and ultrastructural study. Regeneration is influenced by the animal's age and ambient temperature, but in comparison to that of lizards it is very slow and tends to produce outgrowths that do not reach the length of the original tail. Although microscopically similar to lizard blastemas, the mesenchyme rapidly gives rise to a dense connective tissue that contains few muscle bundles, nerves, and fat cells...
January 22, 2019: Journal of Morphology
Daniel A Montenegro, Luis F Borda, Yineth Neuta, Luz A Gómez, Diana M Castillo, Daniela Loyo, Gloria I Lafaurie
AIM: The aim of the present study was to establish the association between the presence of oral and uro-vaginal microorganisms in the placental membrane and preterm delivery (PTD), the premature rupture of membranes (PRM), and the clinical signs of intra-amniotic infection. METHODS: Eighty-four women with PTD and 127 women with delivery at term were assessed for the PRM, clinical signs of intra-amniotic infection, and the presence of periodontitis. Twenty-seven microorganisms were identified in the placental tissue using nested polymerase chain reaction (PCR)...
January 20, 2019: Journal of Investigative and Clinical Dentistry
Babyla Geraldes Monteiro, Renata Ruoco Loureiro, Priscila Cardoso Cristovam, Joyce Luciana Covre, José Álvaro Pereira Gomes, Irina Kerkis
PURPOSE: To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes. METHODS: We compared the immunophenotypes (ABCG2, K3/12, and vimentin) of cells grown on amniotic membranes or plastic surfaces under serum-free conditions or in culture media containing serum or serum replacement components...
January 2019: Arquivos Brasileiros de Oftalmologia
Yifeng Bian, Xiaojie Ma, Ruixia Wang, Hua Yuan, Ning Chen, Yifei Du
Epidemiological evidence suggests that diabetes mellitus (DM) is an important factor in promoting periodontitis. It not only affects the attachment of connective tissue but also causes loss of alveolar bone. Hence, there is an urgent need to find an effective treatment for DM-induced bone deficiency. This study aimed to investigate the effects of human amniotic mesenchymal stem cells (HAMSCs) on the proliferation and osteogenic differentiation of DM-induced human bone marrow mesenchymal stem cells (HBMSCs)...
January 2019: FEBS Open Bio
I A Marino-Martínez, A G Martínez-Castro, V M Peña-Martínez, C A Acosta-Olivo, F Vílchez-Cavazos, A Guzmán-López, Edelmiro Pérez Rodríguez, V J Romero-Díaz, J A Ortega-Blanco, J Lara-Arias
Osteoarthritis (OA) is a degenerative joint disease that affects the soft tissues and bones of involved articulations as a result of deregulation between synthesis and extracellular matrix degradation in articular cartilage. The present study evaluated the effect of intra-articular injection of human amniotic membrane (AM) as a treatment in an OA animal model in the knee. Chemical OA was developed in the knees of New Zealand rabbits. Once OA was established, the right knees only were treated with an intra-articular injection of human AM, with the left knees considered as a negative control group...
January 2019: Experimental and Therapeutic Medicine
Monika Gasiūnienė, Aistė Zentelytė, Gražina Treigytė, Sandra Baronaitė, Jūratė Savickienė, Algirdas Utkus, Rūta Navakauskienė
Amniotic fluid-derived mesenchymal stem cells (AF-MSCs) are autologous to the fetus and represent a potential alternative source for the regenerative medicine and treatment of perinatal disorders. To date, AF-MSCs differentiation capacity to non-mesodermal lineages and epigenetic regulation are still poorly characterized. The present study investigated the differentiation potential of AF-MSCs towards neural-like cells in comparison to the mesodermal myogenic lineage and assessed epigenetic factors involved in tissue-specific differentiation...
January 11, 2019: Cell Biology International
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"