Ruizhu Zhang, Shuxian Pan, Suya Zheng, Qingqing Liao, Zhaodi Jiang, Dixian Wang, Xuemei Li, Ao Hu, Xinran Li, Yezhang Zhu, Xiaoqi Shen, Jing Lei, Siming Zhong, Xiaomei Zhang, Lingyun Huang, Xiaorong Wang, Lan Huang, Li Shen, Bao-Liang Song, Jing-Wei Zhao, Zhiping Wang, Bing Yang, Xing Guo
Protein degradation in eukaryotic cells is mainly carried out by the 26 S proteasome, a macromolecular complex not only present in the cytosol and nucleus but also associated with various membranes. How proteasomes are anchored to the membrane and the biological meaning thereof have been largely unknown in higher organisms. Here, we show that N -myristoylation of the Rpt2 subunit is a general mechanism for proteasome-membrane interaction. Loss of this modification in the Rpt2-G2A mutant cells leads to profound changes in the membrane-associated proteome, perturbs the endomembrane system, and undermines critical cellular processes such as cell adhesion, endoplasmic reticulum-associated degradation and membrane protein trafficking...
December 2023: Science Advances