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P2X7 and cardiomyocytes

Lu Ding, Chengxin Gong, Jiani Zhao, Xingzi Liu, Tao Li, Shenqiang Rao, Shuo Wang, Yuanyuan Liu, Shanping Peng, Wen Xiao, Chaopeng Xiong, Rumeng Wang, Shangdong Liang, Hong Xu
Increasing evidence has suggested high-fat diet (HFD) is an independent risk factor for myocardial ischemia/reperfusion (MI/R) injury. Long noncoding RNAs (lncRNAs) recently attracted much attraction in the study of MI/R injury. However, the functional questions of specific lncRNAs in HFD-induced MI/R injury have not been well elucidated. Uc.48+ is a lncRNA from a transcribed ultraconserved region (T-UCR) of human, mouse, and rat genomes. Here, we explored the aggravating role of uc.48+and identified purinergic P2X7 receptor (P2X7R) as a downstream regulator of uc...
November 11, 2018: Journal of Cellular Physiology
Safoura Mazrouei, Fatemeh Sharifpanah, Mohamed M Bekhite, Hans-Reiner Figulla, Heinrich Sauer, Maria Wartenberg
Purinergic signaling may be involved in embryonic development of the heart. In the present study, the effects of purinergic receptor stimulation on cardiomyogenesis of mouse embryonic stem (ES) cells were investigated. ADP or ATP increased the number of cardiac clusters and cardiac cells, as well as beating frequency. Cardiac-specific genes showed enhanced expression of α-MHC, MLC2v, α-actinin, connexin 45 (Cx45), and HCN4, on both gene and protein levels upon ADP/ATP treatment, indicating increased cardiomyogenesis and pacemaker cell differentiation...
December 2015: Purinergic Signalling
Yaoping Tang, Yongchao Wang, Kyoung-Mi Park, Qiuping Hu, Jian-Peng Teoh, Zuzana Broskova, Punithavathi Ranganathan, Calpurnia Jayakumar, Jie Li, Huabo Su, Yaoliang Tang, Ganesan Ramesh, Il-Man Kim
AIMS: Cardiac injury is accompanied by dynamic changes in the expression of microRNAs (miRs). For example, miR-150 is down-regulated in patients with acute myocardial infarction, atrial fibrillation, dilated and ischaemic cardiomyopathy as well as in various mouse heart failure (HF) models. Circulating miR-150 has been recently proposed as a better biomarker of HF than traditional clinical markers such as brain natriuretic peptide. We recently showed using the β-arrestin-biased β-blocker, carvedilol that β-arrestin1-biased β1-adrenergic receptor cardioprotective signalling stimulates the processing of miR-150 in the heart...
June 1, 2015: Cardiovascular Research
Kwok-Kuen Cheung, Camila Marques-da-Silva, Leandro Vairo, Danúbia Silva dos Santos, Regina Goldenberg, Robson Coutinho-Silva, Geoffrey Burnstock
Purinergic receptors activated by extracellular nucleotides (adenosine 5'-triphosphate (ATP) and uridine 5'-triphosphate (UTP)) are well known to exert physiological effects on the cardiovascular system, whether nucleotides participate functionally in embryonic heart development is not clear. The responsiveness of embryonic cardiomyocytes (E) 12 to P2 receptor agonists by measuring Ca(2+) influx did not present response to ATP, but responses to P2 agonists were detected in cardiomyocytes taken from E14 and E18 rats...
March 2015: Purinergic Signalling
Shohei Kumagai, Kazuki Matsui, Haruyo Kawaguchi, Tomomi Yamashita, Tomomi Mohri, Yasushi Fujio, Hiroyuki Nakayama
Fibrosis is one of the most common pathological alterations in heart failure, and fibroblast migration is an essential process in the development of cardiac fibrosis. Experimental autoimmune myocarditis (EAM) is a model of inflammatory heart disease characterized by inflammatory cell infiltration followed by healing without residual fibrosis. However, the precise mechanisms mediating termination of inflammation and nonfibrotic healing remain to be elucidated. Microarray analysis of hearts from model mice at multiple time points after EAM induction identified several secreted proteins upregulated during nonfibrotic healing, including the anti-inflammatory cathelicidin antimicrobial peptide (CAMP)...
August 9, 2013: Biochemical and Biophysical Research Communications
Claudia Pfleger, Georg Ebeling, Robert Bläsche, Miranda Patton, Hemal H Patel, Michael Kasper, Kathrin Barth
Caveolae and caveolins, structural components of caveolae, are associated with specific ion channels in cardiac myocytes. We have previously shown that P2X purinoceptor 7 (P2X7R), a ligand-gated ion channel, is increased in atrial cardiomyocytes of caveolin-1 knockout mice; however, the specific biochemical relationship of P2X7R with caveolins in the heart is not clear. The aim of this work was to study the presence of the P2X7R in atrial cardiomyocytes and its biochemical relationship to caveolin-1 and caveolin-3...
August 2012: Histochemistry and Cell Biology
Eleonora Mezzaroma, Stefano Toldo, Daniela Farkas, Ignacio M Seropian, Benjamin W Van Tassell, Fadi N Salloum, Harsha R Kannan, Angela C Menna, Norbert F Voelkel, Antonio Abbate
Acute myocardial infarction (AMI) initiates an intense inflammatory response that promotes cardiac dysfunction, cell death, and ventricular remodeling. The molecular events underlying this inflammatory response, however, are incompletely understood. In experimental models of sterile inflammation, ATP released from dying cells triggers, through activation of the purinergic P2X7 receptor, the formation of the inflammasome, a multiprotein complex necessary for caspase-1 activation and amplification of the inflammatory response...
December 6, 2011: Proceedings of the National Academy of Sciences of the United States of America
K Barth, C Pfleger, A Linge, J A Sim, A Surprenant, N Steinbronn, R H Strasser, M Kasper
It has recently been shown in epithelial cells that the ATP-gated ion channel P2X7R is in part, associated with caveolae and colocalized with caveolin-1. In the present study of the mouse heart, we show for the first time, using immunohistochemistry and cryoimmunoelectron microscopy, that P2X7R is expressed in atrial cardiomyocytes and in cardiac microvascular endothelial cells, but not in the ventricle cardiomyocytes. Furthermore, biochemical data indicate the presence of two forms of P2X7R, the classical glycosylated 80 kDa isoform and a protein with the molecular weight of 56 kDa, in both cardiomyocytes and endothelial cells of the mouse heart...
July 2010: Histochemistry and Cell Biology
S K Khaira, C W Pouton, J M Haynes
BACKGROUND AND PURPOSE: Neurons derived from mouse embryonic stem cells (mESCs) are a valuable resource for basic pharmacological research. With the exception of cardiomyocytes, there is relatively little understanding of the pharmacology of stem cell-derived differentiated cells. In this study we investigate P2 receptor agonist effects on GABAergic neurons derived from mESCs. EXPERIMENTAL APPROACH: mESCs were differentiated into GABAergic neurons in the presence of N2B27 culture medium...
December 2009: British Journal of Pharmacology
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