keyword
https://read.qxmd.com/read/10896718/p2y-purinoceptor-activation-mobilizes-intracellular-ca2-and-induces-a-membrane-current-in-rat-intracardiac-neurones
#21
JOURNAL ARTICLE
D M Liu, C Katnik, M Stafford, D J Adams
1. The mobilization of Ca2+ by purinoceptor activation and the relative contributions of intra- and extracellular sources of Ca2+ were investigated using microfluorimetric measurements of fura-2 loaded in cultured neurones from rat intracardiac ganglia. 2. Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed expression of mRNA for the G protein-coupled P2Y2 and P2Y4 receptors. 3. Brief application of either 300 microM ATP or 300 microM UTP caused transient increases in [Ca2+]i of 277 +/- 22 nM and 267 +/- 39 nM, respectively...
July 15, 2000: Journal of Physiology
https://read.qxmd.com/read/10503935/increase-in-cardiac-p2x1-and-p2y2-receptor-mrna-levels-in-congestive-heart-failure
#22
JOURNAL ARTICLE
M Hou, M Malmsjö, S Möller, E Pantev, A Bergdahl, X H Zhao, X Y Sun, T Hedner, L Edvinsson, D Erlinge
We wanted to study the expression of P2-receptors at the mRNA-level in the heart and if it is affected by congestive heart failure (CHF). To quantify the P2 receptor mRNA-expression we used a competitive RT-PCR protocol which is based on an internal RNA standard. The P2 receptor mRNA-expression was quantified in hearts from CHF rats and compared to sham-operated rats. Furthermore, the presence of receptor mRNA was studied in the myocardium from patients with heart failure. In the sham operated rats the G-protein coupled P2Y-receptors were expressed at a higher level than the ligand gated ion-channel receptor (P2X1)...
1999: Life Sciences
https://read.qxmd.com/read/9647463/molecular-cloning-and-characterization-of-rat-p2y4-nucleotide-receptor
#23
JOURNAL ARTICLE
Y D Bogdanov, S S Wildman, M P Clements, B F King, G Burnstock
An intronless open reading frame encoding a protein (361aa in length) was isolated from a rat genomic library probed with a DNA fragment from rat heart. This protein showed 83% sequence identity with the human P2Y4 (hP2Y4) receptor and represents a homologue of the human pyrimidinoceptor. However, the rP2Y4 receptor is not selective for uridine nucleotides and, instead, shows an agonist potency order of ITP = ATP = ADP(pure) = UTP = ATPgammaS = 2-MeSATP = Ap4A > UDP(pure). ADP, ATPgammaS, 2-MeSATP and UDP are partial agonists...
June 1998: British Journal of Pharmacology
https://read.qxmd.com/read/9489506/characterisation-of-subtypes-of-the-p2x-and-p2y-families-of-atp-receptors-in-the-foetal-human-heart
#24
JOURNAL ARTICLE
Y Bogdanov, A Rubino, G Burnstock
ATP exerts a variety of actions within the myocardium, including the regulation of coronary vascular tone and modulation of the autonomic control of the heart. In order to characterise the ATP receptor subtypes involved in these effects, degenerate oligonucleotides were used to clone receptors of both P2X and P2Y families from the human foetal heart. About 1 ng of "Quick-Clone cDNA" from foetal human heart was subjected to amplification with two pairs of degenerate oligonucleotides designed to amplify subtypes of the P2X and P2Y receptor families by means of PCR reactions...
1998: Life Sciences
https://read.qxmd.com/read/9131403/molecular-biology-of-p2y-purinoceptors-expression-in-rat-heart
#25
JOURNAL ARTICLE
T E Webb, M O Boluyt, E A Barnard
1. Application of molecular biology to the study of P2Y purinoceptors has led to the identification of seven such receptors. Here we briefly review their properties and investigate qualitatively the expression of four rat receptor transcripts in heart. 2. The reverse transcriptase-polymerase chain reaction was used to ascertain whether the rat P2Y1, P2Y2, P2Y4 and P2Y6 receptor transcripts were expressed in whole heart, neonatal cardiac fibroblasts, neonatal cardiac myocytes and adult cardiac myocytes. 3. All receptor sequences could be amplified from neonatal rat whole heart, with P2Y6 appearing the most abundant transcript of the four...
December 1996: Journal of Autonomic Pharmacology
https://read.qxmd.com/read/7724657/purinoceptors-are-there-families-of-p2x-and-p2y-purinoceptors
#26
REVIEW
M P Abbracchio, G Burnstock
There has been an exponential growth in interest in purinoceptors since the potent effects of purines were first reported in 1929 and purinoceptors defined in 1978. A distinction between P1 (adenosine) and P2 (ATP/ADP) purinoceptors was recognized at that time and later, A1 and A2, as well as P2x and P2y subclasses of P1 and P2 purinoceptors were also defined. However, in recent years, many new subclasses have been claimed, particularly for the receptors to nucleotides, including P2t, P2z, P2u(n) and P2D, and there is some confusion now about how to incorporate additional discoveries concerning the responses of different tissues to purines...
1994: Pharmacology & Therapeutics
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