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Paul D Gamlin, John J Alexander, Sanford L Boye, C Douglas Witherspoon, Shannon E Boye
Adeno-associated virus (AAV) has emerged as the vector of choice for delivering genes to the retina. Indeed, the first gene therapy to receive FDA approval in the United States is an AAV-based treatment for the inherited retinal disease, Leber congenital amaurosis-2. Voretigene neparvovec (Luxturna™) is delivered to patients via subretinal (SR) injection, an invasive surgical procedure that requires detachment of photoreceptors (PRs) from the retinal pigment epithelium (RPE). It has been reported that subretinal administration of vector under the cone-exclusive fovea leads to a loss of central retinal structure and visual acuity in some patients...
2019: Methods in Molecular Biology
Daniel Su, Anthony Obeid, Jason Hsu
Hydration of retinal tissue has been proposed as a potential model of macular hole (MH) formation in addition to tractional forces of the vitreous and internal limiting membrane (ILM). Carbonic anhydrase inhibitors have previously been utilized in the treatment of cystoid macular edema in outer retinal diseases. There has been recent interest in the use of topical aqueous suppression as a potential medical therapy for MHs. In this case series, four eyes with small (< 300 μm) full-thickness MHs were treated with topical dorzolamide-timolol for 1 month...
February 1, 2019: Ophthalmic Surgery, Lasers & Imaging Retina
Ogugua N Okonkwo, Michael E Gyasi, Adekunle O Hassan, Olufemi Oderinlo
Recent advances in macular hole surgery have significantly improved the rates of anatomical success. One important advance is the dye-assisted peeling of the internal limiting membrane (ILM) around the hole. In large holes (<400 μ), sometimes, ILM peeling alone is not enough to achieve closure, and this may be improved by inverting part of the peeled membrane and using it to cover the macular hole or inverting and tucking the ILM flap into the hole. In this case report, a patient with large full-thickness macular hole underwent a hole repair with an inverted ILM flap technique...
July 2018: Middle East African Journal of Ophthalmology
Justis P Ehlers, Atsuro Uchida, Sunil K Srivastava, Ming Hu
Purpose: To establish a predictive model of macular hole (MH) closure speed. Methods: This study was a post hoc analysis of eyes that underwent full-thickness MH repair in the prospective PIONEER intraoperative optical coherence tomography (iOCT) study. The Bioptigen SDOIS system was used for iOCT imaging. All patients underwent standard small-gauge vitrectomy with internal limiting membrane (ILM) peeling, gas tamponade, and postoperative facedown positioning. Before vitrectomy and after ILM peeling, various quantitative OCT measures related to MH were obtained, including MH geometry alterations and outer retinal features...
January 2019: Translational Vision Science & Technology
Rino Frisina, Raffaele Parrozzani, Elisabetta Pilotto, Edoardo Midena
OBJECTIVES: To propose a new technique of a double inverted epiretinal membrane (ERM) and internal limiting membrane (ILM) flap (double flap group) for the treatment of idiopathic lamellar macular hole (LMH) with atypical ERM (AERM) and to compare surgical outcomes to those of the standard technique of ERM and ILM peeling of the fovea including LMH (control group). METHODS: AERM was tomographically defined as a medium reflective thickened membrane above the retinal fiber layers without retinal traction signs...
February 8, 2019: Ophthalmologica. Journal International D'ophtalmologie
Emrah Özcan, S Selvin Selvi, Emrah Nikerel, Bas Teusink, Ebru Toksoy Öner, Tunahan Çakır
Leuconostoc mesenteroides subsp. cremoris is an obligate heterolactic fermentative lactic acid bacterium that is mostly used in industrial dairy fermentations. The phosphoketolase pathway (PKP) is a unique feature of the obligate heterolactic fermentation, which leads to the production of lactate, ethanol, and/or acetate, and the final product profile of PKP highly depends on the energetics and redox state of the organism. Another characteristic of the L. mesenteroides subsp. cremoris is the production of aroma compounds in dairy fermentation, such as in cheese production, through the utilization of citrate...
February 2, 2019: Applied Microbiology and Biotechnology
Christian Zoller, Alwin Kienle
A software for fast rendering the visual appearance of a blood vessel located in human skin was developed based on a numerical solution of the radiative transfer equation. The user can specify geometrical properties, such as the depth and the diameter of the vessel, and physiological properties, such as the oxygen saturation of the vessel or the blood concentration in the skin. From these data, the spatially and spectrally resolved reflectance from the skin containing the blood vessel is calculated via Monte Carlo simulations, by which a two-dimensional image is generated...
January 2019: Journal of Biomedical Optics
Song Xia, Xin-Yu Zhao, Er-Qian Wang, You-Xin Chen
BACKGROUND: A few randomized controlled trials (RCTs) have evaluated face-down posturing (FDP) with the far less physically challenging nonsupine posturing (NSP) in the treatment of idiopathic full-thickness macular holes (MHs). The objective of our study was to evaluate the efficacy of postoperative posturing on the anatomical and functional outcomes of MH surgery. METHODS: The PubMed, Embase, and Cochrane Central Register of Controlled Trials databases were searched from their earliest entries through December 2016 to identify the studies that had evaluated the effects of postoperative posturing with FDP or NSP for patients with MH surgery...
January 28, 2019: BMC Ophthalmology
Renato Menezes Palácios, Andre Maia, Michel Eid Farah, Mauricio Maia
PURPOSES: To compare surgeons' opinions regarding idiopathic full-thickness macular hole (MH) surgery by using traditional microscopy and three-dimensional (3-D) visualization system. To analyze the required time for pars plana vitrectomy (PPV) and for internal limiting membrane (ILM) rhexis by using both visualization methods. To evaluate anatomical surgical results. METHODS: Four surgeons (surgeon 1, fellows 1, 2, 3) performed the total of 40 surgeries for treating MHs...
January 23, 2019: International Ophthalmology
Y Y Chen, N Lu, J P Li, J Yu, Y J Yu, X Y Shi
Objective: To evaluate the results of pars plana vitrectomy (PPV) with peeling of the inner limiting membrane (ILM) in patients with laser-induced macular hole. Methods: The clinical data of 11 patients (12 eyes) with decreased visual acuity due to laser-induced macular hole from January 2014 to December 2017 were retrospectively studied.All patients underwent routine examination, fundus photography and optical coherence tomography (OCT). Patients were observed every two weeks. PPV with ILM peeling was performed when macular hole was enlarged...
December 25, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Renato Menezes Palácios, André Corrêa Maia de Carvalho, Maurício Maia, Rafael Ramos Caiado, Danilo Aedo Gardim Camilo, Michel Eid Farah
PURPOSE: To evaluate the initial experiences of several vitreoretinal surgeons in Brazil, both experienced and beginners, with a three-dimensional (3D) system, and to report the advantages and disadvantages of this technology. We also report surgical manipulations performed using the heads-up method in porcine eyes. For full-thickness idiopathic macular holes (MHs), we analyzed the times required for pars plana vitrectomy (PPV) and internal limiting membrane (ILM) rhexis by using traditional microscopy and 3D system, and to evaluate anatomical surgical results...
January 15, 2019: Graefe's Archive for Clinical and Experimental Ophthalmology
Karen Peynshaert, Joke Devoldere, An-Katrien Minnaert, Stefaan C De Smedt, Katrien Remaut
The inner limiting membrane (ILM) represents the structural boundary between the vitreous and the retina, and is suggested to act as a barrier for a wide range of retinal therapies. While it is widely acknowledged that the morphology of the human ILM exhibits regional variations and undergoes age-related changes, insight into its structure in laboratory animals is very limited. Besides presenting a detailed overview of the morphology and composition of the human ILM, this review specifically reflects on the species-specific differences in ILM structure...
January 14, 2019: Current Eye Research
Rafael R Caiado, Cristiane Peris, Eduardo B Rodrigues, Michel Eid Farah, André Maia, Octaviano Magalhães, Eduardo Novais, Acácio Souza Lima-Filho, Mauricio Maia
PURPOSE: To test the applicability of the acai dye at a 25% concentration for identifying the posterior hyaloids and internal limiting membranes (ILMs) during pars plana vitrectomy (PPV) in human eyes with macular holes (MHs). METHODS: This study included 25 patients with chronic idiopathic MHs. The exclusion criteria included glaucoma, previous significant ocular conditions, and previous ocular surgeries except uncomplicated cataract. Ten surgeons performed 23-gauge four-port PPV, phacoemulsification, posterior hyaloid detachment, ILM peeling guided by dye staining, and perfluoropropane injection...
January 3, 2019: Graefe's Archive for Clinical and Experimental Ophthalmology
Yuichi Ozaki, Hector M Garcia-Garcia, Alexandre Hideo-Kajita, Kayode O Kuku, Michael Haude, Hüseyin Ince, Alexandre Abizaid, Ralph Tölg, Pedro Alves Lemos, Clemens von Birgelen, Evald Høj Christiansen, William Wijns, Javier Escaned, Jouke Dijkstra, Ron Waksman
Aims: Second-generation drug-eluting absorbable metal scaffold (DREAMS 2G) is an alternative novel device for treating coronary lesions. However, the relationship between in-scaffold dimensions after implantation of DREAMS 2G and vessel healing and luminal results at follow-up is unknown. The aim of this study is, therefore, to investigate whether the expansion index after implantation of DREAMS 2G as assessed by optical coherence tomography (OCT) impacts late luminal status and healing of the vessel wall...
December 26, 2018: European Heart Journal Cardiovascular Imaging
Filomena Napolitano, Valentina Di Iorio, Giuseppe Di Iorio, Mariarosa Anna Beatrice Melone, Fernando Gianfrancesco, Francesca Simonelli, Teresa Esposito, Francesco Testa, Simone Sampaolo
BACKGROUND: Extracellular matrix molecular components, previously linked to multisystem syndromes include collagens, fibrillins and laminins. Recently, we described a novel multisystem syndrome caused by the c.9418G>A p.(V3140M) mutation in the laminin alpha-5 (LAMA5) gene, which affects connective tissues of all organs and apparatus in a three generation family. In the same family, we have also reported a myopic trait, which, however, was linked to the Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) gene...
December 27, 2018: Ophthalmic Genetics
Wenyi Liu, Mingming Liu, Yong Liu, Shiying Li, Chuanhuang Weng, Yan Fu, Juncai He, Yu Gong, Weiping Liu, CongJian Zhao, ZhengQin Yin
To study whether ectopic human melanopsin (hMel) in retinal ganglion cells (RGCs) could restore the visual function in end-stage retinal degeneration, AAV2/8-CMV-hMel/FYP was injected into the intravitreal space of Royal College of Surgeons (RCS) rats. We observed that ectopic hMel/YFP was dominantly expressed in the RGCs of the RCS rat retinae. At 30-45 d after administration of AAV2/8-CMV-hMel/FYP in RCS rats, the FVEP and behavioral results demonstrated that visual function was significantly improved compared with that in the control group, while no improvement in FERG was observed at this time point...
December 22, 2018: Human Gene Therapy
Luyi Zhang, Xiaoxia Li, Yu Shen, Xiaoqin Lin, Miaoqin Wu
In this study, we investigated the pro-proliferative, pro-transdifferentiation effects and mechanisms of nerve growth factor (NGF), internal limiting membrane (ILM), and NGF+ILM on Müller cells. The Müller cells cultured with NGF, ILM or both were mediated with tyrosine kinase A (TrkA) (a high affinity receptor for NGF) inhibitor, phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) (an intracellular signaling pathway important in regulating the cell cycle) inhibitor, or LIN28 (a RNA-binding protein and a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells) siRNA...
December 19, 2018: Experimental Eye Research
Nicolas A Yannuzzi, Xiao Yi Zhou, Pedro Monsalve, Sander R Dubovy, William E Smiddy
This is a report of a 31-year-old woman with unilateral vision loss due to serous retinal detachment and schisis-like changes associated with an optic disc pit. The patient was initially observed but worsened clinically and underwent pars plana vitrectomy (PPV) with internal limiting membrane (ILM) peeling, laser photocoagulation, and removal of an optic pit plug. Histopathological analysis of the specimen disclosed that the plug consisted of fibrovascular neural tissue. [Ophthalmic Surg Lasers Imaging Retina...
December 1, 2018: Ophthalmic Surgery, Lasers & Imaging Retina
David R P Almeida, Eric K Chin, Parnian Arjmand, Gabriel Velez, Lucy P Evans, Vinit B Mahajan
BACKGROUND AND OBJECTIVE: To describe a novel surgical technique using pars plana vitrectomy (PPV), internal limiting membrane (ILM) abrasion, and intravitreal fibrin glue for the treatment of optic disc pit maculopathy. PATIENTS AND METHODS: Surgical case series technique with scanning electron microscopy (SEM) of human post-mortem eyes. RESULTS: Using SEM, the authors demonstrate the persistent adherence of vitreous fragments to the optic disc following induction of posterior vitreous detachment in human postmortem eyes...
December 1, 2018: Ophthalmic Surgery, Lasers & Imaging Retina
Raghav Ravani, Atul Kumar, R Karthikeya, Pradeep Kumar, Yogita Gupta, Vinit Mutha, Shilky Singh
BACKGROUND AND OBJECTIVE: To evaluate long-term outcomes of inverted internal limiting membrane (ILM) stuffing into the optic disc pit for optic disc pit maculopathy (ODP-M) compared with vitrectomy with ILM peeling alone. PATIENTS AND METHODS: Twelve eyes of 12 patients who underwent vitrectomy for ODP-M and followed up more than 12 months were included. We retrospectively analyzed outcomes of inverted ILM flap stuffing into ODP (group 1, n = 6) with ILM peeling alone (group 2, n = 6)...
December 1, 2018: Ophthalmic Surgery, Lasers & Imaging Retina
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