keyword
https://read.qxmd.com/read/38526880/dominance-complementation-of-parental-heading-date-alleles-of-hd1-ghd7-dth8-and-prr37-confers-transgressive-late-maturation-in-hybrid-rice
#1
JOURNAL ARTICLE
Wubei Zong, Yingang Song, Dongdong Xiao, Xiaotong Guo, Fuquan Li, Kangli Sun, Wenjing Tang, Wenhao Xie, Yanqiu Luo, Shan Liang, Jingyao Zhou, Xianrong Xie, Dilin Liu, Letian Chen, Haiyang Wang, Yao-Guang Liu, Jingxin Guo
Rice (Oryza sativa L.) is a short-day plant whose heading date is largely determined by photoperiod sensitivity (PS). Many parental lines used in hybrid rice breeding have weak PS, but their F1 progenies have strong PS and exhibit an undesirable transgressive late-maturing phenotype. However, the genetic basis for this phenomenon is unclear. Therefore, effective methods are needed for selecting parents to create F1 hybrid varieties with the desired PS. In this study, we used bulked segregant analysis with F1 Ningyou 1179 (strong PS) and its F2 population, and through analyzing both parental haplotypes and PS data for 918 hybrid rice varieties, to identify the genetic basis of transgressive late maturation which is dependent on dominance complementation effects of Hd1, Ghd7, DTH8, and PRR37 from both parents rather than from a single parental genotype...
March 25, 2024: Plant Journal
https://read.qxmd.com/read/38523559/diverse-inositol-phosphorylceramide-synthase-mutant-alleles-of-physcomitrium-patens-offer-new-insight-into-complex-sphingolipid-metabolism
#2
JOURNAL ARTICLE
Tegan M Haslam, Cornelia Herrfurth, Ivo Feussner
Sphingolipids are widespread, abundant, and essential lipids in plants and in other eukaryotes. Glycosyl inositol phosphorylceramides (GIPCs) are the most abundant class of plant sphingolipids, and are enriched in the plasma membrane of plant cells. They have been difficult to study due to lethal or pleiotropic mutant phenotypes. To overcome this, we developed a CRISPR/Cas9-based method for generating multiple and varied knockdown and knockout populations of mutants in a given gene of interest in the model moss Physcomitrium patens...
March 25, 2024: New Phytologist
https://read.qxmd.com/read/38520529/impact-of-biotic-stresses-on-the-brassicaceae-family-and-opportunities-for-crop-improvement-by-exploiting-genotyping-traits
#3
REVIEW
Shayani Das Laha, Avijit Kundu, Soumita Podder
Utilizing RNAi, miRNA, siRNA, lncRNA and exploiting genotyping traits can help safeguard the food supply from illnesses and pest damage to Brassicas, as well as reduce yield losses caused by plant pathogens and insect pests. In the natural environment, plants face significant challenges in the form of biotic stress, due to various living organisms, leading to biological stress and a sharp decline in crop yields. To cope with these effects, plants have evolved specialized mechanisms to mitigate these challenges...
March 23, 2024: Planta
https://read.qxmd.com/read/38520090/efficient-scar-free-knock-ins-of-several-kilobases-in-plants-by-engineered-crispr-cas-endonucleases
#4
JOURNAL ARTICLE
Tom Schreiber, Anja Prange, Petra Schäfer, Thomas Iwen, Ramona Grützner, Sylvestre Marillonnet, Aurélie Lepage, Maire Javelle, Wyatt Paul, Alain Tissier
In plants and mammals, non-homologous end-joining is the dominant pathway to repair DNA double strand breaks, making it challenging to generate knock-in events. We identified two groups of exonucleases from the Herpes Virus and the bacteriophage T7 families that conferred an up to 38-fold increase in HDR frequencies when fused to Cas9/Cas12a in a Tobacco mosaic virus-based transient assay in Nicotiana benthamiana. We achieved precise and scar-free insertion of several kilobases of DNA both in transient and stable transformation systems...
March 22, 2024: Molecular Plant
https://read.qxmd.com/read/38518280/crispring-edr1-harmonizing-grapevine-defense-and-growth-dynamics
#5
JOURNAL ARTICLE
Ritu Singh
No abstract text is available yet for this article.
March 22, 2024: Plant Physiology
https://read.qxmd.com/read/38515251/the-mir6445-nac029-module-regulates-drought-tolerance-by-regulating-the-expression-of-glutathione-s-transferase-u23-and-reactive-oxygen-species-scavenging-in-populus
#6
JOURNAL ARTICLE
Meng-Xue Niu, Cong-Hua Feng, Fang He, Han Zhang, Yu Bao, Shu-Jing Liu, Xiao Liu, Yanyan Su, Chao Liu, Hou-Ling Wang, Weilun Yin, Xinli Xia
MicroRNAs are essential in plant development and stress resistance, but their specific roles in drought stress require further investigation. Here, we have uncovered that a Populus-specific microRNAs (miRNA), miR6445, targeting NAC (NAM, ATAF, and CUC) family genes, is involved in regulating drought tolerance of poplar. The expression level of miR6445 was significantly upregulated under drought stress; concomitantly, seven targeted NAC genes showed significant downregulation. Silencing the expression of miR6445 by short tandem target mimic technology significantly decreased the drought tolerance in poplar...
March 21, 2024: New Phytologist
https://read.qxmd.com/read/38513731/a-duf966-gene-family-member-osdsr3-positively-regulates-alkali-stress-tolerance-in-rice
#7
JOURNAL ARTICLE
Xuping Lu, Yan Li, Maryam Akhtar, Caixia Liu, Tianli Ma, Weifang Min, Xiaorong Bai, Yangmengfei She, Liang Chen, Lei Tian, Peifu Li, Chengke Luo
Rice growth and production are severely constrained by alkali stress. However, the mechanism underlying the rice tolerance to alkali stress is unclear. OsDSR3, a novel gene from the domains of unknown function 966 (DUF966) family, was identified and characterized for its function in the response of rice to alkali stress. The result of this study clearly showed that alkali stress significantly induced OsDSR3 expression level. Moreover, the expression of OsDSR3 was up-regulated by drought, salt, cold, H2 O2 and abscisic acid (ABA), and down-regulated by gibberellic acid (GA3 ), and 2,4-Dichlorophenoxyacetic acid (2,4-D) treatments...
March 19, 2024: Plant Science: An International Journal of Experimental Plant Biology
https://read.qxmd.com/read/38511622/editing-the-nuclear-localization-signals-of-e1-and-e1lb-enables-the-production-of-tropical-soybean-in-temperate-growing-regions
#8
JOURNAL ARTICLE
Yang Gao, Yuguo Zhang, Chuanyu Ma, Yanhui Chen, Chunxia Liu, Yanli Wang, Songyuan Wang, Xi Chen
Soybean is a typical short-day crop, and most commercial soybean cultivars are restricted to a relatively narrow range of latitudes due to photoperiod sensitivity. Photoperiod sensitivity hinders the utilization of soybean germplasms across geographical regions. When grown in temperate regions, tropical soybean responds to prolonged day length by increasing the vegetative growth phase and delaying flowering and maturity, which often pushes the harvest window past the first frost date. In this study, we used CRISPR/LbCas12a to edit a North American subtropical soybean cultivar named 06KG218440 that belongs to maturity group 5...
March 21, 2024: Plant Biotechnology Journal
https://read.qxmd.com/read/38507576/chimeric-mutations-in-grapevine-enhanced-disease-resistance1-improve-resistance-to-powdery-mildew-without-growth-penalty
#9
JOURNAL ARTICLE
Xue-Na Yu, Ye Guo, Qianling Yang, Haiyan Yu, Meng-Jiao Lu, Liang Zhao, Zhuo-Shuai Jin, Xiang-Nan Xu, Jia-Yue Feng, Ying-Qiang Wen
Grapevine (Vitis vinifera L.) incurs severequality degradation and yield loss from powdery mildew, a major fungal disease caused by Erysiphe necator. ENHANCED DISEASE RESISTANCE1 (EDR1), a Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK), negatively regulates defense responses against powdery mildew in Arabidopsis (Arabidopsis thaliana). However, little is known about the role of the putatively orthologous EDR1 gene in grapevine. In this study, we obtained grapevine VviEDR1-edited lines using CRISPR/Cas9...
March 20, 2024: Plant Physiology
https://read.qxmd.com/read/38504900/mutating-alfalfa-coumarate-3-hydroxylase-using-multiplex-crispr-cas9-leads-to-reduced-lignin-deposition-and-improved-forage-quality
#10
JOURNAL ARTICLE
Tezera W Wolabu, Kashif Mahmood, Fang Chen, Ivone Torres-Jerez, Michael Udvardi, Million Tadege, Lili Cong, Zengyu Wang, Jiangqi Wen
Alfalfa ( Medicago sativa L.) forage quality is adversely affected by lignin deposition in cell walls at advanced maturity stages. Reducing lignin content through RNA interference or antisense approaches has been shown to improve alfalfa forage quality and digestibility. We employed a multiplex CRISPR/Cas9-mediated gene-editing system to reduce lignin content and alter lignin composition in alfalfa by targeting the COUMARATE 3-HYDROXYLASE (MsC3H) gene, which encodes a key enzyme in lignin biosynthesis. Four guide RNAs (gRNAs) targeting the first exon of MsC3H were designed and clustered into a tRNA-gRNA polycistronic system and introduced into tetraploid alfalfa via Agrobacterium -mediated transformation...
2024: Frontiers in Plant Science
https://read.qxmd.com/read/38501140/establishment-and-application-of-agrobacterium-delivered-crispr-cas9-system-for-wild-tobacco-nicotiana-alata-genome-editing
#11
JOURNAL ARTICLE
Cheng Yuan, Jianmin Zeng, Yong Liu, Haiqin Yu, Zhijun Tong, Jianduo Zhang, Qian Gao, Zhong Wang, Xueyi Sui, Bingguang Xiao, Changjun Huang
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR-Cas9) system has been widely applied in cultivated crops, but limited in their wild relatives. Nicotiana alata is a typical wild species of genus Nicotiana that is globally distributed as a horticultural plant and well-studied as a self-incompatibility model. It also has valuable genes for disease resistance and ornamental traits. However, it lacks an efficient genetic transformation and genome editing system, which hampers its gene function and breeding research...
2024: Frontiers in Plant Science
https://read.qxmd.com/read/38500114/new-improvements-in-grapevine-genome-editing-high-efficiency-biallelic-homozygous-knock-out-from-regenerated-plantlets-by-using-an-optimized-zcas9i
#12
JOURNAL ARTICLE
Jérémy Villette, Fatma Lecourieux, Eliot Bastiancig, Marie-Claire Héloir, Benoit Poinssot
BACKGROUND: For ten years, CRISPR/cas9 system has become a very useful tool for obtaining site-specific mutations on targeted genes in many plant organisms. This technology opens up a wide range of possibilities for improved plant breeding in the future. In plants, the CRISPR/Cas9 system is mostly used through stable transformation with constructs that allow for the expression of the Cas9 gene and sgRNA. Numerous studies have shown that site-specific mutation efficiency can vary greatly between different plant species due to factors such as plant transformation efficiency, Cas9 expression, Cas9 nucleotide sequence, the addition of intronic sequences, and many other parameters...
March 18, 2024: Plant Methods
https://read.qxmd.com/read/38499939/a-critical-review-of-conventional-and-modern-approaches-to-develop-herbicide-resistance-in-rice
#13
REVIEW
Shifei Sang, Yanan Wang, Guoqin Yao, Tengyun Ma, Xiaohan Sun, Yijing Zhang, Nan Su, Xiaoyu Tan, Hafiz Muhammad Khalid Abbas, Shengdong Ji, Qamar U Zaman
Together with rice, weeds strive for nutrients and space in farmland, resulting in reduced rice yield and quality. Planting herbicide-resistant rice varieties is one of the effective ways to control weeds. In recent years, a series of breakthroughs have been made to generate herbicide-resistant germplasm, especially the emergence of biotechnological tools such as gene editing, which provides an inherent advantage for the knock-out or knock-in of the desired genes. In order to develop herbicide-resistant rice germplasm resources, gene manipulation has been conducted to enhance the herbicide tolerance of rice varieties through the utilization of techniques such as physical and chemical mutagenesis, as well as genome editing...
2024: Physiologia Plantarum
https://read.qxmd.com/read/38499776/one-step-creation-of-cms-lines-using-a-bocenh3-based-haploid-induction-system-in-brassica-crop
#14
JOURNAL ARTICLE
Fengqing Han, Xiaoli Zhang, Yuxiang Liu, Yumei Liu, Hong Zhao, Zhansheng Li
Heterosis utilization in a large proportion of crops depends on the use of cytoplasmic male sterility (CMS) tools, requiring the development of homozygous fertile lines and CMS lines1 . Although doubled haploid (DH) technology has been developed for several crops to rapidly generate fertile lines2,3 , CMS lines are generally created by multiple rounds of backcrossing, which is time consuming and expensive4 . Here we describe a method for generating both homozygous fertile and CMS lines through in vivo paternal haploid induction (HI)...
March 18, 2024: Nature Plants
https://read.qxmd.com/read/38498220/crispr-cas9-mediated-knockout-of-bsr-d1-enhances-the-blast-resistance-of-rice-in-northeast-china
#15
JOURNAL ARTICLE
Ying Zhang, Xiu-Feng Lin, Li Li, Ri-Hua Piao, Songquan Wu, Anqi Song, Mengmeng Gao, Yong-Mei Jin
The blast resistance allele of OsBsr-d1 does not exist in most japonica rice varieties of Jilin Province in China. The development of Bsr-d1 knockout mutants via CRISPR/Cas9 enhances broad-spectrum resistance to rice blast in Northeast China. Rice blast is a global disease that has a significant negative impact on rice yield and quality. Due to the complexity and variability of the physiological races of rice blast, controlling rice blast is challenging in agricultural production. Bsr-d1, a negative transcription factor that confers broad-spectrum resistance to rice blast, was identified in the indica rice cultivar Digu; however, its biological function in japonica rice varieties is still unclear...
March 18, 2024: Plant Cell Reports
https://read.qxmd.com/read/38495029/crispr-cas9-revolutionizes-macleaya-cordata-breeding-a-leap-in-sanguinarine-biosynthesis
#16
JOURNAL ARTICLE
Mengshan Sun, Xiaohong Zhong, Li Zhou, Wei Liu, Rong Song, Peng Huang, Jianguo Zeng
No abstract text is available yet for this article.
March 2024: Horticulture Research
https://read.qxmd.com/read/38494539/crop-bioengineering-via-gene-editing-reshaping-the-future-of-agriculture
#17
REVIEW
Mohamed Atia, Wenjun Jiang, Khalid Sedeek, Haroon Butt, Magdy Mahfouz
Genome-editing technologies have revolutionized research in plant biology, with major implications for agriculture and worldwide food security, particularly in the face of challenges such as climate change and increasing human populations. Among these technologies, clustered regularly interspaced short palindromic repeats [CRISPR]-CRISPR-associated protein [Cas] systems are now widely used for editing crop plant genomes. In this review, we provide an overview of CRISPR-Cas technology and its most significant applications for improving crop sustainability...
March 18, 2024: Plant Cell Reports
https://read.qxmd.com/read/38493663/redundant-role-of-oscngc4-and-oscngc5-encoding-cyclic-nucleotide-gated-channels-in-rice-pollen-germination-and-tube-growth
#18
JOURNAL ARTICLE
Eun Young Kim, Myung-Hee Kim, Sang Dae Yun, Su-Kyoung Lee, Eui-Jung Kim, Ji-Hyun Kim, Sung-Aeong Oh, Yu-Jin Kim, Ki-Hong Jung, Soon Ki Park
In staple crops, such as rice (Oryza sativa L.), pollen plays a crucial role in seed production. However, the molecular mechanisms underlying rice pollen germination and tube growth remain underexplored. Notably, we recently uncovered the redundant expression and mutual interaction of two rice genes encoding cyclic nucleotide-gated channels (CNGCs), OsCNGC4 and OsCNGC5, in mature pollen. Building on these findings, the current study focused on clarifying the functional roles of these two genes in pollen germination and tube growth...
March 11, 2024: Plant Physiology and Biochemistry: PPB
https://read.qxmd.com/read/38492487/acyl-coa-synthetase-1-plays-an-important-role-on-pollen-development-and-male-fertility-in-tomato
#19
JOURNAL ARTICLE
Yin-Ge Xie, Yao Xiao, Meng-Yi Yu, Wen-Cai Yang
The development of pollen is critical to male reproduction in flowering plants. Acyl-CoA synthetase (ACOS) genes play conserved functions in regulating pollen development in various plants. Our previous work found that knockout of the SlACOS1 gene in tomato might decrease fruit setting. The current study further revealed that SlACOS1 was important to pollen development and male fertility. The SlACOS1 gene was preferentially expressed in the stamen of the flower with the highest expression at the tetrad stage of anther development...
March 11, 2024: Plant Physiology and Biochemistry: PPB
https://read.qxmd.com/read/38492234/mutl-homolog-1-participates-in-interference-sensitive-meiotic-crossover-formation-in-soybean
#20
JOURNAL ARTICLE
Tao Wu, Suxin Yang, Junling Fang, Yongheng Ye, Yaohua Zhang, Jinshan Gao, Jiantian Leng, Zhirui Zhang, Kuanqiang Tang, Javaid Akhter Bhat, Xianzhong Feng
MutL homolog 1 (MLH1), a member of the MutL-homolog family, is required for normal recombination in most organisms. However, its role in soybean (Glycine max) remains unclear to date. Here, we characterized the Glycine max female and male sterility 1 (Gmfms1) mutation that reduces pollen grain viability and increases embryo sac abortion in soybean. Map-based cloning revealed that the causal gene of Gmfms1 is Glycine max MutL homolog 1 (GmMLH1), and CRISPR/Cas9 knockout approach further validated that disruption of GmMLH1 confers the female-male sterility phenotype in soybean...
March 16, 2024: Plant Physiology
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