Jess D Hebert, Haiqing Xu, Yuning J Tang, Paloma A Ruiz, Colin Detrick, Jing Wang, Nicholas W Hughes, Oscar Donosa, Laura Andrejka, Saswati Karmakar, Irenosen Aboiralor, Le Cong, Julien Sage, Dmitri A Petrov, Monte M Winslow
Somatic genome editing in mouse models has increased our understanding of the in vivo effects of genetic alterations in areas ranging from neuroscience to cancer biology and beyond. However, existing models have been restricted in their ability to create multiple targeted edits, which has limited investigations into complex genetic interactions that underlie development, homeostasis, and disease. To accelerate and expand the generation of complex genotypes in somatic cells, we generated transgenic mice with Cre-regulated and constitutive expression of enhanced Acidaminococcus sp...
March 8, 2024: bioRxiv